Experiment: reconstituted MRS with Amylopectin from maize 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14845 and BBR_RS14850 are separated by 139 nucleotides BBR_RS14850 and BBR_RS20870 are separated by 132 nucleotides BBR_RS20870 and BBR_RS14860 are separated by 148 nucleotides
BBR_RS14845: BBR_RS14845 - permease, at 1,105,442 to 1,106,051
_RS14845
BBR_RS14850: BBR_RS14850 - VOC family protein, at 1,106,191 to 1,106,553
_RS14850
BBR_RS20870: BBR_RS20870 - hypothetical protein, at 1,106,686 to 1,106,865
_RS20870
BBR_RS14860: BBR_RS14860 - MFS transporter, at 1,107,014 to 1,108,384
_RS14860
Position (kb)
1106
1107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1105.716 kb on - strand, within BBR_RS14845 at 1105.765 kb on + strand, within BBR_RS14845 at 1105.817 kb on - strand, within BBR_RS14845 at 1105.832 kb on - strand, within BBR_RS14845 at 1105.914 kb on - strand, within BBR_RS14845 at 1105.918 kb on + strand, within BBR_RS14845 at 1105.952 kb on + strand, within BBR_RS14845 at 1106.043 kb on + strand at 1106.046 kb on - strand at 1106.090 kb on - strand at 1106.152 kb on - strand at 1106.177 kb on - strand at 1106.189 kb on + strand at 1106.189 kb on + strand at 1106.194 kb on + strand at 1106.202 kb on - strand at 1106.204 kb on + strand at 1106.243 kb on + strand, within BBR_RS14850 at 1106.391 kb on - strand, within BBR_RS14850 at 1106.489 kb on - strand, within BBR_RS14850 at 1106.529 kb on - strand at 1106.537 kb on - strand at 1106.569 kb on - strand at 1106.685 kb on - strand at 1106.726 kb on + strand, within BBR_RS20870 at 1106.801 kb on + strand, within BBR_RS20870 at 1106.885 kb on + strand at 1106.888 kb on - strand at 1106.933 kb on + strand at 1106.933 kb on + strand at 1106.946 kb on - strand at 1106.968 kb on - strand at 1106.988 kb on + strand at 1107.000 kb on + strand at 1107.010 kb on + strand at 1107.050 kb on - strand at 1107.050 kb on - strand at 1107.079 kb on + strand at 1107.118 kb on - strand at 1107.244 kb on - strand, within BBR_RS14860 at 1107.253 kb on - strand, within BBR_RS14860 at 1107.267 kb on + strand, within BBR_RS14860 at 1107.270 kb on - strand, within BBR_RS14860 at 1107.291 kb on - strand, within BBR_RS14860 at 1107.296 kb on + strand, within BBR_RS14860 at 1107.296 kb on + strand, within BBR_RS14860 at 1107.296 kb on + strand, within BBR_RS14860 at 1107.355 kb on - strand, within BBR_RS14860 at 1107.377 kb on - strand, within BBR_RS14860 at 1107.388 kb on - strand, within BBR_RS14860 at 1107.406 kb on - strand, within BBR_RS14860 at 1107.451 kb on - strand, within BBR_RS14860 at 1107.531 kb on - strand, within BBR_RS14860 at 1107.559 kb on - strand, within BBR_RS14860 at 1107.564 kb on - strand, within BBR_RS14860 at 1107.576 kb on + strand, within BBR_RS14860 at 1107.675 kb on + strand, within BBR_RS14860 at 1107.779 kb on - strand, within BBR_RS14860 at 1107.791 kb on + strand, within BBR_RS14860 at 1107.791 kb on + strand, within BBR_RS14860 at 1107.803 kb on + strand, within BBR_RS14860 at 1107.803 kb on + strand, within BBR_RS14860 at 1107.811 kb on - strand, within BBR_RS14860
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Amylopectin from maize 10 mg/ml remove 1,105,716 - BBR_RS14845 0.45 +0.9 1,105,765 + BBR_RS14845 0.53 -1.3 1,105,817 - BBR_RS14845 0.61 -0.4 1,105,832 - BBR_RS14845 0.64 -1.1 1,105,914 - BBR_RS14845 0.77 -1.7 1,105,918 + BBR_RS14845 0.78 -1.5 1,105,952 + BBR_RS14845 0.84 -0.9 1,106,043 + +0.7 1,106,046 - -1.3 1,106,090 - +0.7 1,106,152 - -1.8 1,106,177 - +1.1 1,106,189 + +0.3 1,106,189 + -0.5 1,106,194 + +0.6 1,106,202 - -0.5 1,106,204 + -0.5 1,106,243 + BBR_RS14850 0.14 +0.6 1,106,391 - BBR_RS14850 0.55 +0.6 1,106,489 - BBR_RS14850 0.82 +0.1 1,106,529 - +0.3 1,106,537 - +1.1 1,106,569 - +2.1 1,106,685 - +0.1 1,106,726 + BBR_RS20870 0.22 -2.8 1,106,801 + BBR_RS20870 0.64 -1.5 1,106,885 + -0.4 1,106,888 - -0.1 1,106,933 + +0.3 1,106,933 + -0.3 1,106,946 - -2.4 1,106,968 - +0.0 1,106,988 + +1.7 1,107,000 + +0.7 1,107,010 + +0.6 1,107,050 - -1.2 1,107,050 - +0.2 1,107,079 + +0.2 1,107,118 - +0.2 1,107,244 - BBR_RS14860 0.17 -1.4 1,107,253 - BBR_RS14860 0.17 +0.9 1,107,267 + BBR_RS14860 0.18 +1.0 1,107,270 - BBR_RS14860 0.19 -0.4 1,107,291 - BBR_RS14860 0.20 +1.2 1,107,296 + BBR_RS14860 0.21 -0.1 1,107,296 + BBR_RS14860 0.21 +0.1 1,107,296 + BBR_RS14860 0.21 -0.4 1,107,355 - BBR_RS14860 0.25 -2.2 1,107,377 - BBR_RS14860 0.26 -0.3 1,107,388 - BBR_RS14860 0.27 -0.7 1,107,406 - BBR_RS14860 0.29 +0.4 1,107,451 - BBR_RS14860 0.32 -0.1 1,107,531 - BBR_RS14860 0.38 +0.7 1,107,559 - BBR_RS14860 0.40 -0.2 1,107,564 - BBR_RS14860 0.40 +0.4 1,107,576 + BBR_RS14860 0.41 +0.3 1,107,675 + BBR_RS14860 0.48 -0.6 1,107,779 - BBR_RS14860 0.56 -0.1 1,107,791 + BBR_RS14860 0.57 +0.7 1,107,791 + BBR_RS14860 0.57 +0.0 1,107,803 + BBR_RS14860 0.58 +1.0 1,107,803 + BBR_RS14860 0.58 +0.2 1,107,811 - BBR_RS14860 0.58 -0.8
Or see this region's nucleotide sequence