Experiment: reconstituted MRS with Amylopectin from maize 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14805 and BBR_RS14810 are separated by 55 nucleotides BBR_RS14810 and BBR_RS14815 are separated by 318 nucleotides
BBR_RS14805: BBR_RS14805 - NagC family transcriptional regulator, at 1,096,017 to 1,096,991
_RS14805
BBR_RS14810: BBR_RS14810 - ROK family protein, at 1,097,047 to 1,098,231
_RS14810
BBR_RS14815: BBR_RS14815 - D-hexose-6-phosphate mutarotase, at 1,098,550 to 1,099,389
_RS14815
Position (kb)
1097
1098
1099 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1096.067 kb on + strand at 1096.077 kb on + strand at 1096.085 kb on - strand at 1096.247 kb on - strand, within BBR_RS14805 at 1096.269 kb on + strand, within BBR_RS14805 at 1096.329 kb on - strand, within BBR_RS14805 at 1096.396 kb on + strand, within BBR_RS14805 at 1096.428 kb on + strand, within BBR_RS14805 at 1096.441 kb on + strand, within BBR_RS14805 at 1096.451 kb on + strand, within BBR_RS14805 at 1096.539 kb on - strand, within BBR_RS14805 at 1096.557 kb on + strand, within BBR_RS14805 at 1096.597 kb on + strand, within BBR_RS14805 at 1096.624 kb on - strand, within BBR_RS14805 at 1096.631 kb on + strand, within BBR_RS14805 at 1096.707 kb on + strand, within BBR_RS14805 at 1096.729 kb on + strand, within BBR_RS14805 at 1096.742 kb on + strand, within BBR_RS14805 at 1096.802 kb on - strand, within BBR_RS14805 at 1096.802 kb on - strand, within BBR_RS14805 at 1097.056 kb on + strand at 1097.056 kb on + strand at 1097.063 kb on - strand at 1097.064 kb on - strand at 1097.128 kb on - strand at 1097.128 kb on - strand at 1097.128 kb on - strand at 1097.128 kb on - strand at 1097.139 kb on + strand at 1097.145 kb on + strand at 1097.154 kb on - strand at 1097.155 kb on - strand at 1097.157 kb on + strand at 1097.159 kb on - strand at 1097.188 kb on - strand, within BBR_RS14810 at 1097.269 kb on - strand, within BBR_RS14810 at 1097.271 kb on - strand, within BBR_RS14810 at 1097.271 kb on - strand, within BBR_RS14810 at 1097.279 kb on - strand, within BBR_RS14810 at 1097.286 kb on - strand, within BBR_RS14810 at 1097.287 kb on + strand, within BBR_RS14810 at 1097.326 kb on + strand, within BBR_RS14810 at 1097.326 kb on + strand, within BBR_RS14810 at 1097.350 kb on - strand, within BBR_RS14810 at 1097.411 kb on + strand, within BBR_RS14810 at 1097.419 kb on - strand, within BBR_RS14810 at 1097.428 kb on - strand, within BBR_RS14810 at 1097.430 kb on - strand, within BBR_RS14810 at 1097.446 kb on - strand, within BBR_RS14810 at 1097.446 kb on - strand, within BBR_RS14810 at 1097.470 kb on - strand, within BBR_RS14810 at 1097.496 kb on - strand, within BBR_RS14810 at 1097.503 kb on + strand, within BBR_RS14810 at 1097.522 kb on + strand, within BBR_RS14810 at 1097.537 kb on + strand, within BBR_RS14810 at 1097.542 kb on + strand, within BBR_RS14810 at 1097.550 kb on - strand, within BBR_RS14810 at 1097.555 kb on - strand, within BBR_RS14810 at 1097.555 kb on - strand, within BBR_RS14810 at 1097.568 kb on + strand, within BBR_RS14810 at 1097.633 kb on + strand, within BBR_RS14810 at 1097.651 kb on - strand, within BBR_RS14810 at 1097.664 kb on - strand, within BBR_RS14810 at 1097.702 kb on + strand, within BBR_RS14810 at 1097.702 kb on + strand, within BBR_RS14810 at 1097.702 kb on + strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.710 kb on - strand, within BBR_RS14810 at 1097.712 kb on - strand, within BBR_RS14810 at 1097.851 kb on - strand, within BBR_RS14810 at 1097.901 kb on - strand, within BBR_RS14810 at 1097.935 kb on + strand, within BBR_RS14810 at 1097.992 kb on - strand, within BBR_RS14810 at 1098.064 kb on - strand, within BBR_RS14810 at 1098.102 kb on - strand, within BBR_RS14810 at 1098.107 kb on + strand, within BBR_RS14810 at 1098.131 kb on + strand at 1098.139 kb on - strand at 1098.245 kb on - strand at 1098.281 kb on - strand at 1098.294 kb on - strand at 1098.294 kb on - strand at 1098.528 kb on + strand at 1098.619 kb on + strand at 1098.633 kb on - strand at 1098.646 kb on + strand, within BBR_RS14815 at 1098.730 kb on + strand, within BBR_RS14815 at 1098.738 kb on - strand, within BBR_RS14815 at 1098.744 kb on + strand, within BBR_RS14815 at 1098.748 kb on - strand, within BBR_RS14815 at 1098.857 kb on + strand, within BBR_RS14815 at 1098.885 kb on + strand, within BBR_RS14815 at 1098.885 kb on + strand, within BBR_RS14815 at 1098.895 kb on - strand, within BBR_RS14815 at 1098.918 kb on - strand, within BBR_RS14815 at 1098.969 kb on - strand, within BBR_RS14815 at 1099.019 kb on + strand, within BBR_RS14815 at 1099.029 kb on - strand, within BBR_RS14815 at 1099.037 kb on - strand, within BBR_RS14815 at 1099.039 kb on - strand, within BBR_RS14815 at 1099.107 kb on - strand, within BBR_RS14815 at 1099.139 kb on - strand, within BBR_RS14815 at 1099.190 kb on + strand, within BBR_RS14815 at 1099.214 kb on + strand, within BBR_RS14815 at 1099.214 kb on + strand, within BBR_RS14815 at 1099.222 kb on - strand, within BBR_RS14815 at 1099.222 kb on - strand, within BBR_RS14815 at 1099.231 kb on - strand, within BBR_RS14815
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Amylopectin from maize 10 mg/ml remove 1,096,067 + -0.5 1,096,077 + -0.6 1,096,085 - +0.1 1,096,247 - BBR_RS14805 0.24 -2.1 1,096,269 + BBR_RS14805 0.26 +0.2 1,096,329 - BBR_RS14805 0.32 +1.4 1,096,396 + BBR_RS14805 0.39 +0.4 1,096,428 + BBR_RS14805 0.42 +0.2 1,096,441 + BBR_RS14805 0.43 +0.7 1,096,451 + BBR_RS14805 0.45 -1.3 1,096,539 - BBR_RS14805 0.54 +0.1 1,096,557 + BBR_RS14805 0.55 +1.3 1,096,597 + BBR_RS14805 0.59 -0.1 1,096,624 - BBR_RS14805 0.62 -2.1 1,096,631 + BBR_RS14805 0.63 -0.2 1,096,707 + BBR_RS14805 0.71 -0.8 1,096,729 + BBR_RS14805 0.73 -0.6 1,096,742 + BBR_RS14805 0.74 +1.0 1,096,802 - BBR_RS14805 0.81 -0.1 1,096,802 - BBR_RS14805 0.81 -0.4 1,097,056 + -2.2 1,097,056 + -0.0 1,097,063 - +1.4 1,097,064 - -0.9 1,097,128 - -1.0 1,097,128 - +0.4 1,097,128 - -0.1 1,097,128 - -1.0 1,097,139 + -0.4 1,097,145 + -0.3 1,097,154 - -1.3 1,097,155 - -1.0 1,097,157 + -2.2 1,097,159 - -1.8 1,097,188 - BBR_RS14810 0.12 -0.4 1,097,269 - BBR_RS14810 0.19 -1.0 1,097,271 - BBR_RS14810 0.19 -0.8 1,097,271 - BBR_RS14810 0.19 -1.0 1,097,279 - BBR_RS14810 0.20 -1.3 1,097,286 - BBR_RS14810 0.20 -1.6 1,097,287 + BBR_RS14810 0.20 -2.0 1,097,326 + BBR_RS14810 0.24 -2.1 1,097,326 + BBR_RS14810 0.24 -0.8 1,097,350 - BBR_RS14810 0.26 +0.2 1,097,411 + BBR_RS14810 0.31 +0.1 1,097,419 - BBR_RS14810 0.31 -2.9 1,097,428 - BBR_RS14810 0.32 -1.4 1,097,430 - BBR_RS14810 0.32 -2.5 1,097,446 - BBR_RS14810 0.34 -1.0 1,097,446 - BBR_RS14810 0.34 -1.0 1,097,470 - BBR_RS14810 0.36 -1.1 1,097,496 - BBR_RS14810 0.38 -1.9 1,097,503 + BBR_RS14810 0.38 -1.7 1,097,522 + BBR_RS14810 0.40 -2.0 1,097,537 + BBR_RS14810 0.41 -0.5 1,097,542 + BBR_RS14810 0.42 +0.8 1,097,550 - BBR_RS14810 0.42 +0.1 1,097,555 - BBR_RS14810 0.43 -1.6 1,097,555 - BBR_RS14810 0.43 -1.6 1,097,568 + BBR_RS14810 0.44 -0.3 1,097,633 + BBR_RS14810 0.49 -0.9 1,097,651 - BBR_RS14810 0.51 -2.4 1,097,664 - BBR_RS14810 0.52 -1.3 1,097,702 + BBR_RS14810 0.55 -2.2 1,097,702 + BBR_RS14810 0.55 +0.3 1,097,702 + BBR_RS14810 0.55 -0.8 1,097,710 - BBR_RS14810 0.56 -2.5 1,097,710 - BBR_RS14810 0.56 -1.2 1,097,710 - BBR_RS14810 0.56 -2.2 1,097,710 - BBR_RS14810 0.56 -1.4 1,097,710 - BBR_RS14810 0.56 -2.5 1,097,710 - BBR_RS14810 0.56 -0.9 1,097,712 - BBR_RS14810 0.56 -2.1 1,097,851 - BBR_RS14810 0.68 -0.6 1,097,901 - BBR_RS14810 0.72 -0.8 1,097,935 + BBR_RS14810 0.75 -2.5 1,097,992 - BBR_RS14810 0.80 -0.2 1,098,064 - BBR_RS14810 0.86 -1.0 1,098,102 - BBR_RS14810 0.89 -2.1 1,098,107 + BBR_RS14810 0.89 -0.9 1,098,131 + -1.9 1,098,139 - -2.1 1,098,245 - -0.3 1,098,281 - -0.8 1,098,294 - +0.9 1,098,294 - +0.9 1,098,528 + -0.1 1,098,619 + +0.2 1,098,633 - -0.5 1,098,646 + BBR_RS14815 0.11 +0.0 1,098,730 + BBR_RS14815 0.21 -0.8 1,098,738 - BBR_RS14815 0.22 +1.1 1,098,744 + BBR_RS14815 0.23 -1.0 1,098,748 - BBR_RS14815 0.24 +0.5 1,098,857 + BBR_RS14815 0.37 -0.7 1,098,885 + BBR_RS14815 0.40 +0.0 1,098,885 + BBR_RS14815 0.40 +0.3 1,098,895 - BBR_RS14815 0.41 -0.6 1,098,918 - BBR_RS14815 0.44 -1.0 1,098,969 - BBR_RS14815 0.50 +0.4 1,099,019 + BBR_RS14815 0.56 -0.5 1,099,029 - BBR_RS14815 0.57 +0.1 1,099,037 - BBR_RS14815 0.58 -0.2 1,099,039 - BBR_RS14815 0.58 +0.3 1,099,107 - BBR_RS14815 0.66 -0.5 1,099,139 - BBR_RS14815 0.70 +1.0 1,099,190 + BBR_RS14815 0.76 +0.5 1,099,214 + BBR_RS14815 0.79 +0.3 1,099,214 + BBR_RS14815 0.79 +0.2 1,099,222 - BBR_RS14815 0.80 +0.1 1,099,222 - BBR_RS14815 0.80 +0.2 1,099,231 - BBR_RS14815 0.81 -0.2
Or see this region's nucleotide sequence