Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24740 and EX31_RS24745 are separated by 161 nucleotides EX31_RS24745 and EX31_RS24750 are separated by 131 nucleotides EX31_RS24750 and EX31_RS24755 are separated by 495 nucleotides
EX31_RS24740: EX31_RS24740 - ATP-dependent RNA helicase DbpA, at 718,120 to 719,502
_RS24740
EX31_RS24745: EX31_RS24745 - hypothetical protein, at 719,664 to 720,029
_RS24745
EX31_RS24750: EX31_RS24750 - prolyl oligopeptidase family serine peptidase, at 720,161 to 722,200
_RS24750
EX31_RS24755: EX31_RS24755 - prolyl aminopeptidase, at 722,696 to 723,652
_RS24755
Position (kb) 720
721
722
723 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 719.197 kb on + strand, within EX31_RS24740 at 719.198 kb on - strand, within EX31_RS24740 at 719.198 kb on - strand, within EX31_RS24740 at 719.214 kb on + strand, within EX31_RS24740 at 719.283 kb on - strand, within EX31_RS24740 at 719.385 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.405 kb on - strand at 719.405 kb on - strand at 719.467 kb on - strand at 719.491 kb on - strand at 719.572 kb on + strand at 719.576 kb on - strand at 719.602 kb on + strand at 719.602 kb on + strand at 719.603 kb on - strand at 719.603 kb on - strand at 719.606 kb on + strand at 719.613 kb on - strand at 719.855 kb on + strand, within EX31_RS24745 at 719.855 kb on + strand, within EX31_RS24745 at 719.855 kb on + strand, within EX31_RS24745 at 719.933 kb on + strand, within EX31_RS24745 at 719.966 kb on + strand, within EX31_RS24745 at 719.966 kb on + strand, within EX31_RS24745 at 719.967 kb on - strand, within EX31_RS24745 at 720.012 kb on + strand at 720.012 kb on + strand at 720.013 kb on - strand at 720.025 kb on + strand at 720.077 kb on - strand at 720.208 kb on - strand at 720.208 kb on - strand at 720.208 kb on - strand at 720.229 kb on + strand at 720.230 kb on - strand at 720.233 kb on + strand at 720.233 kb on + strand at 720.234 kb on - strand at 720.281 kb on + strand at 720.302 kb on + strand at 720.303 kb on - strand at 720.304 kb on + strand at 720.305 kb on - strand at 720.305 kb on - strand at 720.350 kb on + strand at 720.370 kb on + strand, within EX31_RS24750 at 720.398 kb on + strand, within EX31_RS24750 at 720.399 kb on - strand, within EX31_RS24750 at 720.458 kb on + strand, within EX31_RS24750 at 720.459 kb on - strand, within EX31_RS24750 at 720.617 kb on + strand, within EX31_RS24750 at 720.618 kb on - strand, within EX31_RS24750 at 720.719 kb on + strand, within EX31_RS24750 at 720.719 kb on + strand, within EX31_RS24750 at 720.719 kb on + strand, within EX31_RS24750 at 720.719 kb on + strand, within EX31_RS24750 at 720.720 kb on - strand, within EX31_RS24750 at 720.720 kb on - strand, within EX31_RS24750 at 720.720 kb on - strand, within EX31_RS24750 at 720.720 kb on - strand, within EX31_RS24750 at 720.722 kb on + strand, within EX31_RS24750 at 720.723 kb on - strand, within EX31_RS24750 at 720.723 kb on - strand, within EX31_RS24750 at 720.809 kb on + strand, within EX31_RS24750 at 720.809 kb on + strand, within EX31_RS24750 at 720.810 kb on - strand, within EX31_RS24750 at 720.867 kb on - strand, within EX31_RS24750 at 720.978 kb on - strand, within EX31_RS24750 at 721.007 kb on + strand, within EX31_RS24750 at 721.046 kb on + strand, within EX31_RS24750 at 721.046 kb on + strand, within EX31_RS24750 at 721.046 kb on + strand, within EX31_RS24750 at 721.047 kb on - strand, within EX31_RS24750 at 721.047 kb on - strand, within EX31_RS24750 at 721.048 kb on + strand, within EX31_RS24750 at 721.048 kb on + strand, within EX31_RS24750 at 721.048 kb on + strand, within EX31_RS24750 at 721.049 kb on - strand, within EX31_RS24750 at 721.238 kb on + strand, within EX31_RS24750 at 721.238 kb on + strand, within EX31_RS24750 at 721.238 kb on + strand, within EX31_RS24750 at 721.239 kb on - strand, within EX31_RS24750 at 721.239 kb on - strand, within EX31_RS24750 at 721.239 kb on - strand, within EX31_RS24750 at 721.239 kb on - strand, within EX31_RS24750 at 721.239 kb on - strand, within EX31_RS24750 at 721.241 kb on - strand, within EX31_RS24750 at 721.256 kb on + strand, within EX31_RS24750 at 721.463 kb on + strand, within EX31_RS24750 at 721.535 kb on - strand, within EX31_RS24750 at 721.739 kb on + strand, within EX31_RS24750 at 721.922 kb on + strand, within EX31_RS24750 at 721.923 kb on - strand, within EX31_RS24750 at 721.926 kb on - strand, within EX31_RS24750 at 721.931 kb on + strand, within EX31_RS24750 at 721.931 kb on + strand, within EX31_RS24750 at 721.931 kb on + strand, within EX31_RS24750 at 721.931 kb on + strand, within EX31_RS24750 at 721.931 kb on + strand, within EX31_RS24750 at 721.932 kb on - strand, within EX31_RS24750 at 721.932 kb on - strand, within EX31_RS24750 at 721.932 kb on - strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.933 kb on + strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.934 kb on - strand, within EX31_RS24750 at 721.943 kb on + strand, within EX31_RS24750 at 721.953 kb on - strand, within EX31_RS24750 at 721.985 kb on - strand, within EX31_RS24750 at 722.065 kb on + strand at 722.095 kb on + strand at 722.096 kb on - strand at 722.096 kb on - strand at 722.096 kb on - strand at 722.165 kb on + strand at 722.165 kb on + strand at 722.166 kb on - strand at 722.166 kb on - strand at 722.198 kb on + strand at 722.199 kb on - strand at 722.202 kb on + strand at 722.202 kb on + strand at 722.203 kb on - strand at 722.222 kb on + strand at 722.223 kb on - strand at 722.223 kb on - strand at 722.298 kb on + strand at 722.299 kb on - strand at 722.299 kb on - strand at 722.299 kb on - strand at 722.304 kb on - strand at 722.413 kb on - strand at 722.418 kb on + strand at 722.419 kb on - strand at 722.419 kb on - strand at 722.419 kb on - strand at 722.419 kb on - strand at 722.419 kb on - strand at 722.432 kb on - strand at 722.458 kb on + strand at 722.458 kb on + strand at 722.458 kb on + strand at 722.462 kb on + strand at 722.463 kb on - strand at 722.470 kb on - strand at 722.470 kb on - strand at 722.470 kb on - strand at 722.568 kb on + strand at 722.572 kb on + strand at 722.606 kb on + strand at 722.608 kb on + strand at 722.608 kb on + strand at 722.609 kb on - strand at 722.609 kb on - strand at 722.642 kb on - strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.651 kb on + strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.652 kb on - strand at 722.667 kb on + strand at 722.681 kb on - strand at 722.715 kb on + strand at 722.716 kb on - strand at 722.716 kb on - strand at 722.717 kb on + strand at 722.717 kb on + strand at 722.735 kb on + strand at 722.736 kb on - strand at 722.817 kb on + strand, within EX31_RS24755 at 722.817 kb on + strand, within EX31_RS24755 at 722.818 kb on - strand, within EX31_RS24755 at 722.818 kb on - strand, within EX31_RS24755 at 722.885 kb on + strand, within EX31_RS24755 at 722.886 kb on - strand, within EX31_RS24755 at 722.953 kb on + strand, within EX31_RS24755 at 722.953 kb on + strand, within EX31_RS24755 at 722.953 kb on + strand, within EX31_RS24755 at 722.953 kb on + strand, within EX31_RS24755 at 723.143 kb on + strand, within EX31_RS24755 at 723.187 kb on + strand, within EX31_RS24755 at 723.187 kb on + strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755 at 723.188 kb on - strand, within EX31_RS24755
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 719,197 + EX31_RS24740 0.78 -0.5 719,198 - EX31_RS24740 0.78 -0.2 719,198 - EX31_RS24740 0.78 +0.6 719,214 + EX31_RS24740 0.79 -1.3 719,283 - EX31_RS24740 0.84 +1.0 719,385 + +0.3 719,404 + -1.4 719,404 + +0.8 719,404 + +0.7 719,404 + +1.3 719,404 + -0.2 719,404 + +0.1 719,404 + +1.3 719,405 - +1.8 719,405 - -0.5 719,467 - -0.6 719,491 - -1.2 719,572 + -0.4 719,576 - +0.7 719,602 + -1.1 719,602 + +1.7 719,603 - -0.1 719,603 - +1.0 719,606 + +0.7 719,613 - +0.8 719,855 + EX31_RS24745 0.52 -0.2 719,855 + EX31_RS24745 0.52 -2.2 719,855 + EX31_RS24745 0.52 +1.4 719,933 + EX31_RS24745 0.73 +0.7 719,966 + EX31_RS24745 0.83 +0.3 719,966 + EX31_RS24745 0.83 +1.2 719,967 - EX31_RS24745 0.83 -0.6 720,012 + +0.8 720,012 + -1.9 720,013 - -1.0 720,025 + +0.9 720,077 - +0.2 720,208 - +0.2 720,208 - +0.4 720,208 - +2.9 720,229 + -1.1 720,230 - +3.0 720,233 + +0.0 720,233 + -0.4 720,234 - +1.0 720,281 + +0.5 720,302 + +0.8 720,303 - +0.0 720,304 + +0.2 720,305 - +1.4 720,305 - +0.7 720,350 + +1.0 720,370 + EX31_RS24750 0.10 +1.3 720,398 + EX31_RS24750 0.12 +1.2 720,399 - EX31_RS24750 0.12 +0.1 720,458 + EX31_RS24750 0.15 +1.0 720,459 - EX31_RS24750 0.15 +1.3 720,617 + EX31_RS24750 0.22 -1.1 720,618 - EX31_RS24750 0.22 +2.5 720,719 + EX31_RS24750 0.27 +1.0 720,719 + EX31_RS24750 0.27 +0.1 720,719 + EX31_RS24750 0.27 -2.1 720,719 + EX31_RS24750 0.27 +1.2 720,720 - EX31_RS24750 0.27 +0.3 720,720 - EX31_RS24750 0.27 +0.0 720,720 - EX31_RS24750 0.27 -0.6 720,720 - EX31_RS24750 0.27 -0.1 720,722 + EX31_RS24750 0.28 +1.1 720,723 - EX31_RS24750 0.28 +0.2 720,723 - EX31_RS24750 0.28 -0.4 720,809 + EX31_RS24750 0.32 +0.8 720,809 + EX31_RS24750 0.32 +0.6 720,810 - EX31_RS24750 0.32 -0.1 720,867 - EX31_RS24750 0.35 +0.9 720,978 - EX31_RS24750 0.40 +0.2 721,007 + EX31_RS24750 0.41 -0.4 721,046 + EX31_RS24750 0.43 -1.7 721,046 + EX31_RS24750 0.43 +0.4 721,046 + EX31_RS24750 0.43 +0.3 721,047 - EX31_RS24750 0.43 +1.0 721,047 - EX31_RS24750 0.43 +0.3 721,048 + EX31_RS24750 0.43 -0.2 721,048 + EX31_RS24750 0.43 -0.8 721,048 + EX31_RS24750 0.43 +0.3 721,049 - EX31_RS24750 0.44 +0.3 721,238 + EX31_RS24750 0.53 +0.9 721,238 + EX31_RS24750 0.53 +0.4 721,238 + EX31_RS24750 0.53 +2.7 721,239 - EX31_RS24750 0.53 +0.7 721,239 - EX31_RS24750 0.53 +0.2 721,239 - EX31_RS24750 0.53 +2.0 721,239 - EX31_RS24750 0.53 +1.2 721,239 - EX31_RS24750 0.53 +0.4 721,241 - EX31_RS24750 0.53 +1.0 721,256 + EX31_RS24750 0.54 -0.7 721,463 + EX31_RS24750 0.64 +0.1 721,535 - EX31_RS24750 0.67 -0.4 721,739 + EX31_RS24750 0.77 -0.6 721,922 + EX31_RS24750 0.86 -0.4 721,923 - EX31_RS24750 0.86 -1.3 721,926 - EX31_RS24750 0.87 +0.2 721,931 + EX31_RS24750 0.87 +1.2 721,931 + EX31_RS24750 0.87 +2.0 721,931 + EX31_RS24750 0.87 +0.0 721,931 + EX31_RS24750 0.87 -0.3 721,931 + EX31_RS24750 0.87 +2.6 721,932 - EX31_RS24750 0.87 +2.3 721,932 - EX31_RS24750 0.87 -0.2 721,932 - EX31_RS24750 0.87 +0.7 721,933 + EX31_RS24750 0.87 -1.7 721,933 + EX31_RS24750 0.87 +0.7 721,933 + EX31_RS24750 0.87 -0.4 721,933 + EX31_RS24750 0.87 +1.8 721,933 + EX31_RS24750 0.87 +1.0 721,933 + EX31_RS24750 0.87 +2.0 721,933 + EX31_RS24750 0.87 -1.0 721,933 + EX31_RS24750 0.87 -1.8 721,934 - EX31_RS24750 0.87 +0.4 721,934 - EX31_RS24750 0.87 +0.7 721,934 - EX31_RS24750 0.87 +0.9 721,934 - EX31_RS24750 0.87 +0.9 721,934 - EX31_RS24750 0.87 +0.3 721,934 - EX31_RS24750 0.87 +0.2 721,943 + EX31_RS24750 0.87 +3.0 721,953 - EX31_RS24750 0.88 +0.9 721,985 - EX31_RS24750 0.89 -1.0 722,065 + +1.0 722,095 + +1.0 722,096 - +0.0 722,096 - +0.4 722,096 - -0.1 722,165 + +1.0 722,165 + +0.0 722,166 - +1.0 722,166 - +0.4 722,198 + -1.1 722,199 - +0.2 722,202 + +0.1 722,202 + +2.3 722,203 - +0.3 722,222 + -1.2 722,223 - -1.8 722,223 - -0.2 722,298 + -0.1 722,299 - +1.9 722,299 - -0.4 722,299 - -0.6 722,304 - -1.8 722,413 - +0.1 722,418 + +2.0 722,419 - +0.2 722,419 - +0.2 722,419 - +0.4 722,419 - -0.1 722,419 - +0.1 722,432 - +1.2 722,458 + +1.0 722,458 + +0.2 722,458 + +1.5 722,462 + +0.0 722,463 - +1.0 722,470 - +0.6 722,470 - +0.6 722,470 - -0.2 722,568 + +1.0 722,572 + +0.0 722,606 + +0.5 722,608 + +1.4 722,608 + +2.0 722,609 - +1.1 722,609 - +2.0 722,642 - +1.0 722,651 + +1.0 722,651 + +0.8 722,651 + +0.7 722,651 + +0.5 722,651 + -2.3 722,651 + +0.3 722,651 + -0.9 722,651 + -0.4 722,651 + +0.4 722,652 - +1.0 722,652 - +0.9 722,652 - +0.8 722,652 - -1.2 722,652 - +0.8 722,652 - -0.3 722,652 - -0.2 722,652 - +0.3 722,667 + +0.4 722,681 - +2.3 722,715 + +0.0 722,716 - -2.7 722,716 - -1.1 722,717 + +0.7 722,717 + +0.7 722,735 + +0.3 722,736 - +2.6 722,817 + EX31_RS24755 0.13 +0.6 722,817 + EX31_RS24755 0.13 +1.3 722,818 - EX31_RS24755 0.13 +0.0 722,818 - EX31_RS24755 0.13 +0.4 722,885 + EX31_RS24755 0.20 +1.4 722,886 - EX31_RS24755 0.20 +0.3 722,953 + EX31_RS24755 0.27 +0.2 722,953 + EX31_RS24755 0.27 +2.0 722,953 + EX31_RS24755 0.27 -0.2 722,953 + EX31_RS24755 0.27 -1.0 723,143 + EX31_RS24755 0.47 -0.2 723,187 + EX31_RS24755 0.51 -1.4 723,187 + EX31_RS24755 0.51 +0.8 723,188 - EX31_RS24755 0.51 +1.3 723,188 - EX31_RS24755 0.51 +1.2 723,188 - EX31_RS24755 0.51 -0.3 723,188 - EX31_RS24755 0.51 -0.9 723,188 - EX31_RS24755 0.51 -0.2 723,188 - EX31_RS24755 0.51 +1.6
Or see this region's nucleotide sequence