Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22575 and EX31_RS22580 are separated by 91 nucleotides EX31_RS22580 and EX31_RS22585 overlap by 4 nucleotides EX31_RS22585 and EX31_RS22590 overlap by 1 nucleotides
EX31_RS22575: EX31_RS22575 - copper-exporting P-type ATPase CopA, at 269,714 to 272,257
_RS22575
EX31_RS22580: EX31_RS22580 - Cu(I)-responsive transcriptional regulator, at 272,349 to 272,753
_RS22580
EX31_RS22585: EX31_RS22585 - NfeD family protein, at 272,750 to 273,205
_RS22585
EX31_RS22590: EX31_RS22590 - SPFH/Band 7/PHB domain protein, at 273,205 to 274,125
_RS22590
Position (kb) 272
273 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 271.352 kb on - strand, within EX31_RS22575 at 271.363 kb on + strand, within EX31_RS22575 at 271.364 kb on - strand, within EX31_RS22575 at 271.383 kb on + strand, within EX31_RS22575 at 271.384 kb on - strand, within EX31_RS22575 at 271.384 kb on - strand, within EX31_RS22575 at 271.386 kb on + strand, within EX31_RS22575 at 271.386 kb on + strand, within EX31_RS22575 at 271.387 kb on - strand, within EX31_RS22575 at 271.487 kb on - strand, within EX31_RS22575 at 271.552 kb on + strand, within EX31_RS22575 at 271.552 kb on + strand, within EX31_RS22575 at 271.552 kb on + strand, within EX31_RS22575 at 271.553 kb on - strand, within EX31_RS22575 at 271.553 kb on - strand, within EX31_RS22575 at 271.747 kb on + strand, within EX31_RS22575 at 271.751 kb on - strand, within EX31_RS22575 at 271.931 kb on + strand, within EX31_RS22575 at 271.932 kb on - strand, within EX31_RS22575 at 272.055 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.056 kb on + strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.057 kb on - strand at 272.143 kb on + strand at 272.159 kb on + strand at 272.159 kb on + strand at 272.160 kb on - strand at 272.240 kb on + strand at 272.240 kb on + strand at 272.241 kb on - strand at 272.243 kb on + strand at 272.243 kb on + strand at 272.244 kb on - strand at 272.249 kb on - strand at 272.300 kb on + strand at 272.304 kb on + strand at 272.304 kb on + strand at 272.304 kb on + strand at 272.304 kb on + strand at 272.304 kb on + strand at 272.304 kb on + strand at 272.305 kb on - strand at 272.305 kb on - strand at 272.305 kb on - strand at 272.305 kb on - strand at 272.305 kb on - strand at 272.305 kb on - strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.306 kb on + strand at 272.307 kb on - strand at 272.307 kb on - strand at 272.307 kb on - strand at 272.307 kb on - strand at 272.307 kb on - strand at 272.307 kb on - strand at 272.313 kb on + strand at 272.406 kb on + strand, within EX31_RS22580 at 272.463 kb on + strand, within EX31_RS22580 at 272.463 kb on + strand, within EX31_RS22580 at 272.464 kb on - strand, within EX31_RS22580 at 272.464 kb on - strand, within EX31_RS22580 at 272.464 kb on - strand, within EX31_RS22580 at 272.478 kb on - strand, within EX31_RS22580 at 272.478 kb on - strand, within EX31_RS22580 at 272.522 kb on + strand, within EX31_RS22580 at 272.522 kb on + strand, within EX31_RS22580 at 272.522 kb on + strand, within EX31_RS22580 at 272.522 kb on + strand, within EX31_RS22580 at 272.587 kb on + strand, within EX31_RS22580 at 272.602 kb on + strand, within EX31_RS22580 at 272.602 kb on + strand, within EX31_RS22580 at 272.741 kb on + strand at 272.742 kb on - strand at 272.884 kb on + strand, within EX31_RS22585 at 272.905 kb on + strand, within EX31_RS22585 at 272.910 kb on + strand, within EX31_RS22585 at 272.910 kb on + strand, within EX31_RS22585 at 272.910 kb on + strand, within EX31_RS22585 at 272.910 kb on + strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.911 kb on - strand, within EX31_RS22585 at 272.966 kb on - strand, within EX31_RS22585 at 272.966 kb on - strand, within EX31_RS22585 at 273.114 kb on + strand, within EX31_RS22585 at 273.114 kb on + strand, within EX31_RS22585 at 273.115 kb on - strand, within EX31_RS22585 at 273.173 kb on + strand at 273.173 kb on + strand at 273.173 kb on + strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.174 kb on - strand at 273.206 kb on + strand at 273.207 kb on - strand at 273.207 kb on - strand at 273.292 kb on - strand at 273.292 kb on - strand at 273.304 kb on - strand, within EX31_RS22590 at 273.304 kb on - strand, within EX31_RS22590 at 273.356 kb on + strand, within EX31_RS22590 at 273.357 kb on - strand, within EX31_RS22590 at 273.360 kb on + strand, within EX31_RS22590 at 273.361 kb on - strand, within EX31_RS22590 at 273.538 kb on + strand, within EX31_RS22590 at 273.538 kb on + strand, within EX31_RS22590 at 273.539 kb on - strand, within EX31_RS22590 at 273.539 kb on - strand, within EX31_RS22590 at 273.573 kb on + strand, within EX31_RS22590 at 273.574 kb on - strand, within EX31_RS22590 at 273.691 kb on - strand, within EX31_RS22590
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 271,352 - EX31_RS22575 0.64 +0.8 271,363 + EX31_RS22575 0.65 +1.2 271,364 - EX31_RS22575 0.65 -0.9 271,383 + EX31_RS22575 0.66 -1.0 271,384 - EX31_RS22575 0.66 +0.3 271,384 - EX31_RS22575 0.66 +0.2 271,386 + EX31_RS22575 0.66 -0.3 271,386 + EX31_RS22575 0.66 +0.5 271,387 - EX31_RS22575 0.66 +0.6 271,487 - EX31_RS22575 0.70 -2.5 271,552 + EX31_RS22575 0.72 +1.0 271,552 + EX31_RS22575 0.72 -1.4 271,552 + EX31_RS22575 0.72 +0.3 271,553 - EX31_RS22575 0.72 +2.8 271,553 - EX31_RS22575 0.72 -0.4 271,747 + EX31_RS22575 0.80 +1.2 271,751 - EX31_RS22575 0.80 +0.9 271,931 + EX31_RS22575 0.87 -2.4 271,932 - EX31_RS22575 0.87 +0.9 272,055 + +0.7 272,056 + +0.3 272,056 + -0.1 272,056 + -1.0 272,056 + +2.8 272,056 + +1.4 272,056 + -0.1 272,056 + +1.2 272,056 + -0.4 272,056 + -0.1 272,056 + +0.5 272,056 + -0.1 272,057 - -0.4 272,057 - +1.0 272,057 - -0.6 272,057 - -2.0 272,057 - +1.8 272,057 - -0.3 272,057 - +0.6 272,057 - -0.2 272,057 - +0.6 272,143 + +1.7 272,159 + +0.1 272,159 + -0.7 272,160 - -1.7 272,240 + -0.3 272,240 + +0.4 272,241 - -0.0 272,243 + +0.8 272,243 + +0.8 272,244 - +2.3 272,249 - -1.9 272,300 + +1.1 272,304 + -0.3 272,304 + +0.0 272,304 + -0.2 272,304 + +1.0 272,304 + -0.9 272,304 + -2.2 272,305 - -0.2 272,305 - -1.1 272,305 - -0.1 272,305 - +0.8 272,305 - +0.8 272,305 - -0.6 272,306 + +0.6 272,306 + +1.2 272,306 + +0.1 272,306 + +0.1 272,306 + +0.6 272,306 + +0.3 272,306 + +0.9 272,306 + -0.9 272,306 + +0.5 272,306 + +1.0 272,306 + +0.3 272,307 - -0.2 272,307 - -0.4 272,307 - -0.0 272,307 - +1.2 272,307 - -0.1 272,307 - -1.1 272,313 + -0.2 272,406 + EX31_RS22580 0.14 +0.7 272,463 + EX31_RS22580 0.28 +0.8 272,463 + EX31_RS22580 0.28 +0.7 272,464 - EX31_RS22580 0.28 -0.9 272,464 - EX31_RS22580 0.28 +0.4 272,464 - EX31_RS22580 0.28 -1.2 272,478 - EX31_RS22580 0.32 +0.2 272,478 - EX31_RS22580 0.32 +0.9 272,522 + EX31_RS22580 0.43 +0.6 272,522 + EX31_RS22580 0.43 -0.0 272,522 + EX31_RS22580 0.43 -0.7 272,522 + EX31_RS22580 0.43 +0.6 272,587 + EX31_RS22580 0.59 -0.0 272,602 + EX31_RS22580 0.62 +1.5 272,602 + EX31_RS22580 0.62 +0.4 272,741 + -0.5 272,742 - -0.9 272,884 + EX31_RS22585 0.29 +0.8 272,905 + EX31_RS22585 0.34 +2.1 272,910 + EX31_RS22585 0.35 +1.5 272,910 + EX31_RS22585 0.35 -0.6 272,910 + EX31_RS22585 0.35 +0.0 272,910 + EX31_RS22585 0.35 -0.4 272,911 - EX31_RS22585 0.35 +0.0 272,911 - EX31_RS22585 0.35 +0.4 272,911 - EX31_RS22585 0.35 -0.6 272,911 - EX31_RS22585 0.35 +0.1 272,911 - EX31_RS22585 0.35 -0.7 272,911 - EX31_RS22585 0.35 +0.7 272,911 - EX31_RS22585 0.35 -2.6 272,911 - EX31_RS22585 0.35 +0.3 272,966 - EX31_RS22585 0.47 -0.6 272,966 - EX31_RS22585 0.47 +0.6 273,114 + EX31_RS22585 0.80 -0.9 273,114 + EX31_RS22585 0.80 -0.1 273,115 - EX31_RS22585 0.80 +0.1 273,173 + -0.4 273,173 + +0.0 273,173 + +0.3 273,174 - -0.7 273,174 - +0.6 273,174 - +0.0 273,174 - +0.0 273,174 - -2.2 273,174 - -0.6 273,174 - -1.3 273,174 - +0.6 273,206 + +3.5 273,207 - -1.2 273,207 - +0.0 273,292 - -0.8 273,292 - +0.2 273,304 - EX31_RS22590 0.11 +0.1 273,304 - EX31_RS22590 0.11 +0.4 273,356 + EX31_RS22590 0.16 -0.8 273,357 - EX31_RS22590 0.17 -1.1 273,360 + EX31_RS22590 0.17 -1.7 273,361 - EX31_RS22590 0.17 +0.3 273,538 + EX31_RS22590 0.36 +0.1 273,538 + EX31_RS22590 0.36 -0.8 273,539 - EX31_RS22590 0.36 -0.7 273,539 - EX31_RS22590 0.36 -0.2 273,573 + EX31_RS22590 0.40 +0.3 273,574 - EX31_RS22590 0.40 -0.4 273,691 - EX31_RS22590 0.53 +0.8
Or see this region's nucleotide sequence