Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21140 and EX31_RS21145 are separated by 54 nucleotides EX31_RS21145 and EX31_RS21150 are separated by 650 nucleotides
EX31_RS21140: EX31_RS21140 - AtzE family amidohydrolase, at 871,204 to 872,607
_RS21140
EX31_RS21145: EX31_RS21145 - oxalurate catabolism protein HpxZ, at 872,662 to 873,045
_RS21145
EX31_RS21150: EX31_RS21150 - allantoinase PuuE, at 873,696 to 874,634
_RS21150
Position (kb)
872
873
874 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 871.732 kb on - strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.763 kb on + strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.764 kb on - strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.888 kb on + strand, within EX31_RS21140 at 871.889 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.899 kb on - strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140 at 871.965 kb on + strand, within EX31_RS21140 at 872.009 kb on - strand, within EX31_RS21140 at 872.048 kb on + strand, within EX31_RS21140 at 872.048 kb on + strand, within EX31_RS21140 at 872.049 kb on - strand, within EX31_RS21140 at 872.128 kb on - strand, within EX31_RS21140 at 872.158 kb on + strand, within EX31_RS21140 at 872.158 kb on + strand, within EX31_RS21140 at 872.159 kb on - strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.248 kb on + strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.249 kb on - strand, within EX31_RS21140 at 872.409 kb on + strand, within EX31_RS21140 at 872.410 kb on - strand, within EX31_RS21140 at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.498 kb on + strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.499 kb on - strand at 872.662 kb on - strand at 872.662 kb on - strand at 872.676 kb on + strand at 872.677 kb on - strand at 872.719 kb on + strand, within EX31_RS21145 at 872.719 kb on + strand, within EX31_RS21145 at 872.720 kb on - strand, within EX31_RS21145 at 872.822 kb on - strand, within EX31_RS21145 at 872.873 kb on + strand, within EX31_RS21145 at 872.874 kb on - strand, within EX31_RS21145 at 872.898 kb on + strand, within EX31_RS21145 at 873.168 kb on + strand at 873.182 kb on + strand at 873.182 kb on + strand at 873.249 kb on + strand at 873.249 kb on + strand at 873.250 kb on - strand at 873.288 kb on + strand at 873.289 kb on - strand at 873.289 kb on - strand at 873.436 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.489 kb on + strand at 873.551 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.562 kb on + strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.563 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.566 kb on - strand at 873.570 kb on - strand at 873.744 kb on - strand at 873.775 kb on + strand at 873.776 kb on - strand at 873.877 kb on + strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.878 kb on - strand, within EX31_RS21150 at 873.904 kb on + strand, within EX31_RS21150 at 873.904 kb on + strand, within EX31_RS21150 at 873.905 kb on - strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.908 kb on + strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.909 kb on - strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.910 kb on + strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.911 kb on - strand, within EX31_RS21150 at 873.977 kb on - strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150 at 874.020 kb on + strand, within EX31_RS21150
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 871,732 - EX31_RS21140 0.38 +0.9 871,763 + EX31_RS21140 0.40 +0.4 871,763 + EX31_RS21140 0.40 -0.2 871,763 + EX31_RS21140 0.40 +0.5 871,763 + EX31_RS21140 0.40 +0.3 871,764 - EX31_RS21140 0.40 +0.1 871,764 - EX31_RS21140 0.40 +2.0 871,764 - EX31_RS21140 0.40 +0.8 871,764 - EX31_RS21140 0.40 +0.5 871,888 + EX31_RS21140 0.49 +2.7 871,888 + EX31_RS21140 0.49 -1.2 871,888 + EX31_RS21140 0.49 -0.8 871,889 - EX31_RS21140 0.49 -0.2 871,899 - EX31_RS21140 0.50 +1.0 871,899 - EX31_RS21140 0.50 -0.2 871,899 - EX31_RS21140 0.50 -0.4 871,965 + EX31_RS21140 0.54 -0.1 871,965 + EX31_RS21140 0.54 +0.0 872,009 - EX31_RS21140 0.57 +0.2 872,048 + EX31_RS21140 0.60 +1.5 872,048 + EX31_RS21140 0.60 +1.4 872,049 - EX31_RS21140 0.60 -0.6 872,128 - EX31_RS21140 0.66 -0.2 872,158 + EX31_RS21140 0.68 -0.3 872,158 + EX31_RS21140 0.68 -0.6 872,159 - EX31_RS21140 0.68 +0.3 872,248 + EX31_RS21140 0.74 +1.6 872,248 + EX31_RS21140 0.74 +0.8 872,248 + EX31_RS21140 0.74 -1.4 872,248 + EX31_RS21140 0.74 -0.3 872,248 + EX31_RS21140 0.74 +0.0 872,248 + EX31_RS21140 0.74 -0.3 872,248 + EX31_RS21140 0.74 +0.8 872,249 - EX31_RS21140 0.74 -0.0 872,249 - EX31_RS21140 0.74 -0.2 872,249 - EX31_RS21140 0.74 -0.1 872,249 - EX31_RS21140 0.74 -0.3 872,249 - EX31_RS21140 0.74 +0.5 872,249 - EX31_RS21140 0.74 +0.8 872,249 - EX31_RS21140 0.74 -0.5 872,409 + EX31_RS21140 0.86 -0.2 872,410 - EX31_RS21140 0.86 +1.0 872,498 + -0.5 872,498 + +1.1 872,498 + +0.4 872,498 + -0.9 872,498 + +0.0 872,498 + -0.4 872,498 + -0.4 872,499 - -0.1 872,499 - +0.9 872,499 - +0.7 872,499 - +0.1 872,499 - +1.4 872,499 - -0.1 872,499 - -0.9 872,662 - +0.6 872,662 - -0.0 872,676 + -0.3 872,677 - +0.8 872,719 + EX31_RS21145 0.15 +0.1 872,719 + EX31_RS21145 0.15 +1.4 872,720 - EX31_RS21145 0.15 +0.6 872,822 - EX31_RS21145 0.42 -0.1 872,873 + EX31_RS21145 0.55 +0.2 872,874 - EX31_RS21145 0.55 +1.8 872,898 + EX31_RS21145 0.61 +0.0 873,168 + -0.7 873,182 + -0.0 873,182 + +1.7 873,249 + -0.1 873,249 + -0.9 873,250 - +0.7 873,288 + +0.9 873,289 - -0.4 873,289 - -0.0 873,436 + +0.8 873,489 + -0.5 873,489 + -0.6 873,489 + +2.4 873,489 + -0.0 873,551 + -0.0 873,562 + +0.6 873,562 + -1.6 873,562 + -0.4 873,562 + -0.3 873,562 + +0.5 873,562 + +0.1 873,562 + -0.2 873,563 - -1.0 873,563 - +0.1 873,563 - +0.2 873,563 - -0.0 873,563 - +1.2 873,563 - -0.3 873,563 - +2.4 873,563 - +0.7 873,563 - -0.6 873,563 - -0.2 873,563 - -0.2 873,566 - -2.2 873,566 - -1.3 873,566 - +1.2 873,566 - +0.2 873,566 - -1.7 873,566 - -1.0 873,566 - -0.7 873,566 - +0.2 873,566 - -2.1 873,570 - -1.5 873,744 - -0.3 873,775 + -1.1 873,776 - -0.9 873,877 + EX31_RS21150 0.19 -0.1 873,878 - EX31_RS21150 0.19 -0.1 873,878 - EX31_RS21150 0.19 -1.3 873,878 - EX31_RS21150 0.19 +0.1 873,904 + EX31_RS21150 0.22 -2.3 873,904 + EX31_RS21150 0.22 -0.9 873,905 - EX31_RS21150 0.22 -0.2 873,908 + EX31_RS21150 0.23 +0.5 873,908 + EX31_RS21150 0.23 -0.3 873,908 + EX31_RS21150 0.23 -0.1 873,908 + EX31_RS21150 0.23 -0.4 873,908 + EX31_RS21150 0.23 +0.8 873,908 + EX31_RS21150 0.23 -0.1 873,908 + EX31_RS21150 0.23 +0.8 873,909 - EX31_RS21150 0.23 -0.5 873,909 - EX31_RS21150 0.23 +0.7 873,909 - EX31_RS21150 0.23 -1.6 873,909 - EX31_RS21150 0.23 +0.4 873,909 - EX31_RS21150 0.23 -0.1 873,909 - EX31_RS21150 0.23 -0.7 873,909 - EX31_RS21150 0.23 -0.3 873,909 - EX31_RS21150 0.23 +0.1 873,909 - EX31_RS21150 0.23 +0.3 873,910 + EX31_RS21150 0.23 -0.1 873,910 + EX31_RS21150 0.23 -0.3 873,910 + EX31_RS21150 0.23 -1.1 873,910 + EX31_RS21150 0.23 +0.4 873,910 + EX31_RS21150 0.23 -2.0 873,910 + EX31_RS21150 0.23 +0.8 873,910 + EX31_RS21150 0.23 -0.3 873,911 - EX31_RS21150 0.23 +0.1 873,911 - EX31_RS21150 0.23 +2.4 873,911 - EX31_RS21150 0.23 +0.7 873,911 - EX31_RS21150 0.23 -0.8 873,977 - EX31_RS21150 0.30 +0.1 874,020 + EX31_RS21150 0.35 -0.2 874,020 + EX31_RS21150 0.35 -1.5 874,020 + EX31_RS21150 0.35 +0.8
Or see this region's nucleotide sequence