Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20545 and EX31_RS20550 are separated by 273 nucleotides EX31_RS20550 and EX31_RS20555 are separated by 215 nucleotides EX31_RS20555 and EX31_RS20560 are separated by 127 nucleotides
EX31_RS20545: EX31_RS20545 - IscS subfamily cysteine desulfurase, at 746,761 to 747,930
_RS20545
EX31_RS20550: EX31_RS20550 - Fe-S cluster assembly transcriptional regulator IscR, at 748,204 to 748,698
_RS20550
EX31_RS20555: EX31_RS20555 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 748,914 to 749,651
_RS20555
EX31_RS20560: EX31_RS20560 - inositol-1-monophosphatase, at 749,779 to 750,582
_RS20560
Position (kb) 748
749
750 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 747.938 kb on - strand at 747.938 kb on - strand at 747.938 kb on - strand at 748.015 kb on + strand at 748.016 kb on - strand at 748.016 kb on - strand at 748.016 kb on - strand at 748.043 kb on + strand at 748.043 kb on + strand at 748.116 kb on - strand at 748.116 kb on - strand at 748.116 kb on - strand at 748.116 kb on - strand at 748.206 kb on - strand at 748.227 kb on + strand at 748.227 kb on + strand at 748.227 kb on + strand at 748.228 kb on - strand at 748.228 kb on - strand at 748.228 kb on - strand at 748.228 kb on - strand at 748.305 kb on + strand, within EX31_RS20550 at 748.327 kb on - strand, within EX31_RS20550 at 748.327 kb on - strand, within EX31_RS20550 at 748.327 kb on - strand, within EX31_RS20550 at 748.327 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.330 kb on - strand, within EX31_RS20550 at 748.373 kb on - strand, within EX31_RS20550 at 748.373 kb on - strand, within EX31_RS20550 at 748.378 kb on - strand, within EX31_RS20550 at 748.378 kb on - strand, within EX31_RS20550 at 748.378 kb on - strand, within EX31_RS20550 at 748.378 kb on - strand, within EX31_RS20550 at 748.417 kb on - strand, within EX31_RS20550 at 748.578 kb on - strand, within EX31_RS20550 at 748.591 kb on - strand, within EX31_RS20550 at 748.591 kb on - strand, within EX31_RS20550 at 748.660 kb on - strand at 748.699 kb on - strand at 748.699 kb on - strand at 748.707 kb on + strand at 748.708 kb on - strand at 748.708 kb on - strand at 748.708 kb on - strand at 748.708 kb on - strand at 748.716 kb on - strand at 748.716 kb on - strand at 748.725 kb on - strand at 748.725 kb on - strand at 748.848 kb on + strand at 748.915 kb on + strand at 748.915 kb on + strand at 748.915 kb on + strand at 748.915 kb on + strand at 748.915 kb on + strand at 748.915 kb on + strand at 748.916 kb on - strand at 748.916 kb on - strand at 748.916 kb on - strand at 748.916 kb on - strand at 748.916 kb on - strand at 748.916 kb on - strand at 748.928 kb on + strand at 748.929 kb on - strand at 748.929 kb on - strand at 748.929 kb on - strand at 748.929 kb on - strand at 748.971 kb on + strand at 748.972 kb on - strand at 749.119 kb on + strand, within EX31_RS20555 at 749.134 kb on + strand, within EX31_RS20555 at 749.135 kb on - strand, within EX31_RS20555 at 749.184 kb on + strand, within EX31_RS20555 at 749.184 kb on + strand, within EX31_RS20555 at 749.184 kb on + strand, within EX31_RS20555 at 749.184 kb on + strand, within EX31_RS20555 at 749.185 kb on - strand, within EX31_RS20555 at 749.191 kb on + strand, within EX31_RS20555 at 749.191 kb on + strand, within EX31_RS20555 at 749.191 kb on + strand, within EX31_RS20555 at 749.192 kb on - strand, within EX31_RS20555 at 749.198 kb on + strand, within EX31_RS20555 at 749.198 kb on + strand, within EX31_RS20555 at 749.233 kb on + strand, within EX31_RS20555 at 749.233 kb on + strand, within EX31_RS20555 at 749.234 kb on - strand, within EX31_RS20555 at 749.234 kb on - strand, within EX31_RS20555 at 749.234 kb on - strand, within EX31_RS20555 at 749.263 kb on + strand, within EX31_RS20555 at 749.263 kb on + strand, within EX31_RS20555 at 749.263 kb on + strand, within EX31_RS20555 at 749.263 kb on + strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.264 kb on - strand, within EX31_RS20555 at 749.267 kb on + strand, within EX31_RS20555 at 749.268 kb on - strand, within EX31_RS20555 at 749.268 kb on - strand, within EX31_RS20555 at 749.432 kb on + strand, within EX31_RS20555 at 749.433 kb on - strand, within EX31_RS20555 at 749.433 kb on - strand, within EX31_RS20555 at 749.433 kb on - strand, within EX31_RS20555 at 749.433 kb on - strand, within EX31_RS20555 at 749.459 kb on + strand, within EX31_RS20555 at 749.460 kb on - strand, within EX31_RS20555 at 749.460 kb on - strand, within EX31_RS20555 at 749.513 kb on + strand, within EX31_RS20555 at 749.552 kb on - strand, within EX31_RS20555 at 749.562 kb on + strand, within EX31_RS20555 at 749.622 kb on + strand at 749.622 kb on + strand at 749.622 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.628 kb on + strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.629 kb on - strand at 749.651 kb on + strand at 749.651 kb on + strand at 749.651 kb on + strand at 749.651 kb on + strand at 749.652 kb on - strand at 749.652 kb on - strand at 749.652 kb on - strand at 749.652 kb on - strand at 749.652 kb on - strand at 749.687 kb on + strand at 749.687 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.698 kb on + strand at 749.699 kb on - strand at 750.593 kb on + strand at 750.637 kb on + strand at 750.637 kb on + strand at 750.637 kb on + strand at 750.638 kb on - strand at 750.638 kb on - strand at 750.650 kb on + strand at 750.650 kb on + strand at 750.650 kb on + strand at 750.650 kb on + strand at 750.650 kb on + strand at 750.650 kb on + strand at 750.651 kb on - strand at 750.651 kb on - strand at 750.651 kb on - strand at 750.651 kb on - strand at 750.651 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 747,938 - -0.1 747,938 - -0.3 747,938 - +0.0 748,015 + +1.1 748,016 - +0.2 748,016 - +0.1 748,016 - +0.3 748,043 + +2.1 748,043 + +0.9 748,116 - +0.2 748,116 - -2.9 748,116 - +0.7 748,116 - +0.2 748,206 - +0.5 748,227 + -0.3 748,227 + +2.4 748,227 + -0.0 748,228 - -0.1 748,228 - +0.5 748,228 - +0.2 748,228 - +0.1 748,305 + EX31_RS20550 0.20 -0.6 748,327 - EX31_RS20550 0.25 +0.4 748,327 - EX31_RS20550 0.25 +0.4 748,327 - EX31_RS20550 0.25 -0.6 748,327 - EX31_RS20550 0.25 +0.8 748,330 - EX31_RS20550 0.25 -1.3 748,330 - EX31_RS20550 0.25 -1.7 748,330 - EX31_RS20550 0.25 -1.1 748,330 - EX31_RS20550 0.25 -0.3 748,330 - EX31_RS20550 0.25 -0.1 748,330 - EX31_RS20550 0.25 -0.3 748,330 - EX31_RS20550 0.25 +0.4 748,330 - EX31_RS20550 0.25 -1.0 748,330 - EX31_RS20550 0.25 -2.9 748,373 - EX31_RS20550 0.34 +0.7 748,373 - EX31_RS20550 0.34 -1.7 748,378 - EX31_RS20550 0.35 -0.8 748,378 - EX31_RS20550 0.35 +0.2 748,378 - EX31_RS20550 0.35 -0.5 748,378 - EX31_RS20550 0.35 -1.9 748,417 - EX31_RS20550 0.43 +1.4 748,578 - EX31_RS20550 0.76 -0.3 748,591 - EX31_RS20550 0.78 -2.0 748,591 - EX31_RS20550 0.78 -0.1 748,660 - -0.1 748,699 - -0.4 748,699 - +0.4 748,707 + +1.4 748,708 - +1.3 748,708 - +0.4 748,708 - -0.5 748,708 - -0.4 748,716 - +0.1 748,716 - +1.1 748,725 - -0.3 748,725 - -0.3 748,848 + +0.2 748,915 + +0.4 748,915 + +1.5 748,915 + +0.5 748,915 + -1.0 748,915 + -0.7 748,915 + +0.5 748,916 - +0.4 748,916 - -0.5 748,916 - +0.9 748,916 - +2.4 748,916 - +0.5 748,916 - -0.9 748,928 + +1.8 748,929 - -0.3 748,929 - -0.1 748,929 - -0.6 748,929 - +2.2 748,971 + +2.2 748,972 - +0.6 749,119 + EX31_RS20555 0.28 +0.2 749,134 + EX31_RS20555 0.30 +0.7 749,135 - EX31_RS20555 0.30 +0.2 749,184 + EX31_RS20555 0.37 -0.2 749,184 + EX31_RS20555 0.37 +2.4 749,184 + EX31_RS20555 0.37 +0.6 749,184 + EX31_RS20555 0.37 -0.4 749,185 - EX31_RS20555 0.37 +0.0 749,191 + EX31_RS20555 0.38 +1.3 749,191 + EX31_RS20555 0.38 +0.1 749,191 + EX31_RS20555 0.38 +0.5 749,192 - EX31_RS20555 0.38 +0.7 749,198 + EX31_RS20555 0.38 -0.6 749,198 + EX31_RS20555 0.38 -1.3 749,233 + EX31_RS20555 0.43 +1.2 749,233 + EX31_RS20555 0.43 +0.4 749,234 - EX31_RS20555 0.43 +0.3 749,234 - EX31_RS20555 0.43 -2.0 749,234 - EX31_RS20555 0.43 +0.2 749,263 + EX31_RS20555 0.47 -0.5 749,263 + EX31_RS20555 0.47 -0.5 749,263 + EX31_RS20555 0.47 -0.3 749,263 + EX31_RS20555 0.47 -0.1 749,264 - EX31_RS20555 0.47 +0.0 749,264 - EX31_RS20555 0.47 +0.8 749,264 - EX31_RS20555 0.47 +1.7 749,264 - EX31_RS20555 0.47 +0.3 749,264 - EX31_RS20555 0.47 -1.7 749,264 - EX31_RS20555 0.47 +0.1 749,264 - EX31_RS20555 0.47 +0.5 749,264 - EX31_RS20555 0.47 +0.0 749,264 - EX31_RS20555 0.47 +0.8 749,264 - EX31_RS20555 0.47 +0.4 749,267 + EX31_RS20555 0.48 +0.7 749,268 - EX31_RS20555 0.48 -0.2 749,268 - EX31_RS20555 0.48 +1.1 749,432 + EX31_RS20555 0.70 -0.2 749,433 - EX31_RS20555 0.70 +0.7 749,433 - EX31_RS20555 0.70 -1.4 749,433 - EX31_RS20555 0.70 +0.5 749,433 - EX31_RS20555 0.70 +0.4 749,459 + EX31_RS20555 0.74 -0.5 749,460 - EX31_RS20555 0.74 -1.9 749,460 - EX31_RS20555 0.74 +1.0 749,513 + EX31_RS20555 0.81 +1.3 749,552 - EX31_RS20555 0.86 +0.4 749,562 + EX31_RS20555 0.88 +0.2 749,622 + +0.6 749,622 + +0.7 749,622 + +0.6 749,628 + -0.5 749,628 + -0.2 749,628 + -1.5 749,628 + +0.7 749,628 + +0.2 749,628 + +0.8 749,628 + +0.8 749,628 + +1.4 749,629 - -0.0 749,629 - +1.8 749,629 - -0.3 749,629 - +0.8 749,629 - -0.2 749,629 - -1.1 749,629 - +0.8 749,651 + +0.1 749,651 + +0.4 749,651 + -0.5 749,651 + +0.1 749,652 - +3.4 749,652 - +0.4 749,652 - +0.9 749,652 - -0.0 749,652 - -0.5 749,687 + -0.2 749,687 + -0.2 749,698 + -0.0 749,698 + -0.9 749,698 + -0.6 749,698 + +1.3 749,698 + +0.9 749,698 + -1.3 749,698 + -0.1 749,698 + -0.3 749,699 - +0.6 750,593 + +0.8 750,637 + +0.7 750,637 + -2.1 750,637 + +0.4 750,638 - +0.2 750,638 - +0.3 750,650 + +1.1 750,650 + -0.0 750,650 + +0.2 750,650 + -0.5 750,650 + -0.3 750,650 + +0.8 750,651 - +3.4 750,651 - +0.3 750,651 - +0.7 750,651 - -0.5 750,651 - +0.6
Or see this region's nucleotide sequence