Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15740 and EX31_RS15745 are separated by 27 nucleotides EX31_RS15745 and EX31_RS15750 overlap by 14 nucleotides EX31_RS15750 and EX31_RS15755 are separated by 199 nucleotides
EX31_RS15740: EX31_RS15740 - IS3 family transposase, at 74 to 1,248
_RS15740
EX31_RS15745: EX31_RS15745 - hypothetical protein, at 1,276 to 1,935
_RS15745
EX31_RS15750: EX31_RS15750 - site-specific DNA-methyltransferase, at 1,922 to 2,647
_RS15750
EX31_RS15755: EX31_RS15755 - hypothetical protein, at 2,847 to 3,674
_RS15755
Position (kb)
1
2 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1.253 kb on + strand at 1.253 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.258 kb on + strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.259 kb on - strand at 1.262 kb on + strand at 1.262 kb on + strand at 1.262 kb on + strand at 1.262 kb on + strand at 1.262 kb on + strand at 1.262 kb on + strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.263 kb on - strand at 1.270 kb on + strand at 1.270 kb on + strand at 1.270 kb on + strand at 1.271 kb on - strand at 1.271 kb on - strand at 1.271 kb on - strand at 1.272 kb on + strand at 1.272 kb on + strand at 1.272 kb on + strand at 1.272 kb on + strand at 1.273 kb on - strand at 1.273 kb on - strand at 1.273 kb on - strand at 1.273 kb on - strand at 1.273 kb on - strand at 1.273 kb on - strand at 1.290 kb on + strand at 1.319 kb on - strand at 1.319 kb on - strand at 1.319 kb on - strand at 1.319 kb on - strand at 1.336 kb on - strand at 1.344 kb on + strand, within EX31_RS15745 at 1.345 kb on - strand, within EX31_RS15745 at 1.355 kb on + strand, within EX31_RS15745 at 1.355 kb on + strand, within EX31_RS15745 at 1.355 kb on + strand, within EX31_RS15745 at 1.363 kb on - strand, within EX31_RS15745 at 1.423 kb on - strand, within EX31_RS15745 at 1.484 kb on - strand, within EX31_RS15745 at 1.509 kb on + strand, within EX31_RS15745 at 1.531 kb on + strand, within EX31_RS15745 at 1.532 kb on - strand, within EX31_RS15745 at 1.560 kb on - strand, within EX31_RS15745 at 1.560 kb on - strand, within EX31_RS15745 at 1.560 kb on - strand, within EX31_RS15745 at 1.560 kb on - strand, within EX31_RS15745 at 1.605 kb on - strand, within EX31_RS15745 at 1.605 kb on - strand, within EX31_RS15745 at 1.605 kb on - strand, within EX31_RS15745 at 1.605 kb on - strand, within EX31_RS15745 at 1.611 kb on - strand, within EX31_RS15745 at 1.624 kb on + strand, within EX31_RS15745 at 1.624 kb on + strand, within EX31_RS15745 at 1.625 kb on - strand, within EX31_RS15745 at 1.625 kb on - strand, within EX31_RS15745 at 1.625 kb on - strand, within EX31_RS15745 at 1.626 kb on + strand, within EX31_RS15745 at 1.626 kb on + strand, within EX31_RS15745 at 1.626 kb on + strand, within EX31_RS15745 at 1.626 kb on + strand, within EX31_RS15745 at 1.626 kb on + strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.627 kb on - strand, within EX31_RS15745 at 1.677 kb on + strand, within EX31_RS15745 at 1.722 kb on + strand, within EX31_RS15745 at 1.723 kb on - strand, within EX31_RS15745 at 1.723 kb on - strand, within EX31_RS15745 at 1.737 kb on + strand, within EX31_RS15745 at 1.738 kb on - strand, within EX31_RS15745 at 1.738 kb on - strand, within EX31_RS15745 at 1.758 kb on + strand, within EX31_RS15745 at 1.759 kb on - strand, within EX31_RS15745 at 1.759 kb on - strand, within EX31_RS15745 at 1.803 kb on + strand, within EX31_RS15745 at 1.803 kb on + strand, within EX31_RS15745 at 1.856 kb on + strand, within EX31_RS15745 at 1.857 kb on - strand, within EX31_RS15745 at 1.936 kb on + strand at 1.946 kb on + strand at 1.946 kb on + strand at 1.963 kb on - strand at 1.973 kb on - strand at 1.974 kb on + strand at 1.975 kb on - strand at 1.975 kb on - strand at 1.979 kb on - strand at 2.014 kb on + strand, within EX31_RS15750 at 2.015 kb on - strand, within EX31_RS15750 at 2.015 kb on - strand, within EX31_RS15750 at 2.015 kb on - strand, within EX31_RS15750 at 2.030 kb on + strand, within EX31_RS15750 at 2.030 kb on + strand, within EX31_RS15750 at 2.033 kb on - strand, within EX31_RS15750 at 2.068 kb on - strand, within EX31_RS15750 at 2.076 kb on + strand, within EX31_RS15750 at 2.080 kb on + strand, within EX31_RS15750 at 2.083 kb on + strand, within EX31_RS15750 at 2.106 kb on + strand, within EX31_RS15750 at 2.116 kb on + strand, within EX31_RS15750 at 2.116 kb on + strand, within EX31_RS15750 at 2.183 kb on + strand, within EX31_RS15750 at 2.208 kb on - strand, within EX31_RS15750 at 2.266 kb on + strand, within EX31_RS15750 at 2.266 kb on + strand, within EX31_RS15750 at 2.267 kb on - strand, within EX31_RS15750 at 2.268 kb on + strand, within EX31_RS15750 at 2.268 kb on + strand, within EX31_RS15750 at 2.268 kb on + strand, within EX31_RS15750 at 2.268 kb on + strand, within EX31_RS15750 at 2.269 kb on - strand, within EX31_RS15750 at 2.269 kb on - strand, within EX31_RS15750 at 2.269 kb on - strand, within EX31_RS15750 at 2.269 kb on - strand, within EX31_RS15750 at 2.269 kb on - strand, within EX31_RS15750 at 2.346 kb on + strand, within EX31_RS15750 at 2.351 kb on + strand, within EX31_RS15750 at 2.351 kb on + strand, within EX31_RS15750 at 2.351 kb on + strand, within EX31_RS15750 at 2.351 kb on + strand, within EX31_RS15750 at 2.351 kb on + strand, within EX31_RS15750 at 2.352 kb on - strand, within EX31_RS15750 at 2.353 kb on + strand, within EX31_RS15750 at 2.353 kb on + strand, within EX31_RS15750 at 2.354 kb on - strand, within EX31_RS15750 at 2.354 kb on - strand, within EX31_RS15750 at 2.354 kb on - strand, within EX31_RS15750 at 2.354 kb on - strand, within EX31_RS15750 at 2.453 kb on + strand, within EX31_RS15750 at 2.455 kb on + strand, within EX31_RS15750 at 2.458 kb on - strand, within EX31_RS15750 at 2.491 kb on + strand, within EX31_RS15750 at 2.493 kb on + strand, within EX31_RS15750 at 2.493 kb on + strand, within EX31_RS15750 at 2.493 kb on + strand, within EX31_RS15750 at 2.493 kb on + strand, within EX31_RS15750 at 2.494 kb on - strand, within EX31_RS15750 at 2.494 kb on - strand, within EX31_RS15750 at 2.494 kb on - strand, within EX31_RS15750 at 2.494 kb on - strand, within EX31_RS15750 at 2.496 kb on + strand, within EX31_RS15750 at 2.506 kb on + strand, within EX31_RS15750 at 2.507 kb on - strand, within EX31_RS15750 at 2.507 kb on - strand, within EX31_RS15750 at 2.535 kb on + strand, within EX31_RS15750 at 2.535 kb on + strand, within EX31_RS15750 at 2.535 kb on + strand, within EX31_RS15750 at 2.536 kb on - strand, within EX31_RS15750 at 2.536 kb on - strand, within EX31_RS15750 at 2.554 kb on + strand, within EX31_RS15750 at 2.572 kb on + strand, within EX31_RS15750 at 2.585 kb on + strand at 2.830 kb on + strand at 2.833 kb on - strand at 2.875 kb on + strand at 2.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 1,253 + -0.5 1,253 + +0.6 1,258 + +0.5 1,258 + +0.5 1,258 + -0.2 1,258 + +0.2 1,258 + -0.1 1,258 + +0.9 1,258 + +0.5 1,258 + +0.1 1,258 + +0.2 1,258 + +0.6 1,258 + -0.8 1,258 + +0.4 1,258 + -0.5 1,258 + +1.0 1,258 + -3.2 1,258 + +1.0 1,258 + +0.7 1,258 + -0.1 1,259 - +0.4 1,259 - +1.0 1,259 - +0.9 1,259 - +0.3 1,259 - -0.5 1,259 - +0.3 1,259 - +0.1 1,259 - -0.2 1,262 + -0.7 1,262 + -0.4 1,262 + +2.0 1,262 + +0.0 1,262 + +0.6 1,262 + -0.4 1,263 - +0.6 1,263 - -0.6 1,263 - -0.5 1,263 - -2.2 1,263 - -0.9 1,263 - -0.6 1,263 - -1.3 1,270 + +2.9 1,270 + +1.3 1,270 + -1.0 1,271 - -0.4 1,271 - -0.9 1,271 - +0.6 1,272 + +0.5 1,272 + +0.0 1,272 + -2.0 1,272 + +0.6 1,273 - +0.4 1,273 - +0.1 1,273 - +0.5 1,273 - +0.6 1,273 - +0.2 1,273 - -0.3 1,290 + +0.3 1,319 - +0.3 1,319 - -1.5 1,319 - -1.9 1,319 - -0.0 1,336 - +0.3 1,344 + EX31_RS15745 0.10 -0.7 1,345 - EX31_RS15745 0.10 +1.0 1,355 + EX31_RS15745 0.12 -0.1 1,355 + EX31_RS15745 0.12 -0.2 1,355 + EX31_RS15745 0.12 -0.5 1,363 - EX31_RS15745 0.13 +0.4 1,423 - EX31_RS15745 0.22 -1.2 1,484 - EX31_RS15745 0.32 +0.3 1,509 + EX31_RS15745 0.35 +0.7 1,531 + EX31_RS15745 0.39 +0.8 1,532 - EX31_RS15745 0.39 +0.3 1,560 - EX31_RS15745 0.43 +0.1 1,560 - EX31_RS15745 0.43 +0.4 1,560 - EX31_RS15745 0.43 -0.4 1,560 - EX31_RS15745 0.43 -1.1 1,605 - EX31_RS15745 0.50 -0.1 1,605 - EX31_RS15745 0.50 +0.3 1,605 - EX31_RS15745 0.50 -0.2 1,605 - EX31_RS15745 0.50 +0.6 1,611 - EX31_RS15745 0.51 +1.6 1,624 + EX31_RS15745 0.53 +0.7 1,624 + EX31_RS15745 0.53 +0.5 1,625 - EX31_RS15745 0.53 +0.7 1,625 - EX31_RS15745 0.53 +0.7 1,625 - EX31_RS15745 0.53 -0.0 1,626 + EX31_RS15745 0.53 -0.1 1,626 + EX31_RS15745 0.53 +2.9 1,626 + EX31_RS15745 0.53 +0.1 1,626 + EX31_RS15745 0.53 -0.3 1,626 + EX31_RS15745 0.53 -0.5 1,627 - EX31_RS15745 0.53 +0.7 1,627 - EX31_RS15745 0.53 -1.1 1,627 - EX31_RS15745 0.53 +1.3 1,627 - EX31_RS15745 0.53 -0.8 1,627 - EX31_RS15745 0.53 +1.0 1,627 - EX31_RS15745 0.53 +0.5 1,627 - EX31_RS15745 0.53 -1.0 1,627 - EX31_RS15745 0.53 +0.6 1,627 - EX31_RS15745 0.53 +0.8 1,677 + EX31_RS15745 0.61 -0.3 1,722 + EX31_RS15745 0.68 -0.2 1,723 - EX31_RS15745 0.68 +0.3 1,723 - EX31_RS15745 0.68 +0.5 1,737 + EX31_RS15745 0.70 +0.3 1,738 - EX31_RS15745 0.70 -0.2 1,738 - EX31_RS15745 0.70 -1.6 1,758 + EX31_RS15745 0.73 -0.2 1,759 - EX31_RS15745 0.73 -0.6 1,759 - EX31_RS15745 0.73 -0.4 1,803 + EX31_RS15745 0.80 -1.7 1,803 + EX31_RS15745 0.80 +0.7 1,856 + EX31_RS15745 0.88 +0.6 1,857 - EX31_RS15745 0.88 +0.5 1,936 + -1.2 1,946 + +0.4 1,946 + +0.3 1,963 - +0.4 1,973 - -1.1 1,974 + +0.2 1,975 - +0.1 1,975 - +0.5 1,979 - +0.9 2,014 + EX31_RS15750 0.13 +1.1 2,015 - EX31_RS15750 0.13 -0.2 2,015 - EX31_RS15750 0.13 -1.0 2,015 - EX31_RS15750 0.13 +0.6 2,030 + EX31_RS15750 0.15 +0.8 2,030 + EX31_RS15750 0.15 -0.2 2,033 - EX31_RS15750 0.15 -0.2 2,068 - EX31_RS15750 0.20 +1.4 2,076 + EX31_RS15750 0.21 +1.5 2,080 + EX31_RS15750 0.22 -0.1 2,083 + EX31_RS15750 0.22 -1.3 2,106 + EX31_RS15750 0.25 +0.7 2,116 + EX31_RS15750 0.27 +0.4 2,116 + EX31_RS15750 0.27 +0.3 2,183 + EX31_RS15750 0.36 -0.2 2,208 - EX31_RS15750 0.39 -2.1 2,266 + EX31_RS15750 0.47 +0.4 2,266 + EX31_RS15750 0.47 +0.2 2,267 - EX31_RS15750 0.48 +0.9 2,268 + EX31_RS15750 0.48 -1.4 2,268 + EX31_RS15750 0.48 -0.7 2,268 + EX31_RS15750 0.48 +0.4 2,268 + EX31_RS15750 0.48 +0.9 2,269 - EX31_RS15750 0.48 +0.1 2,269 - EX31_RS15750 0.48 -1.2 2,269 - EX31_RS15750 0.48 +0.5 2,269 - EX31_RS15750 0.48 +0.6 2,269 - EX31_RS15750 0.48 +0.4 2,346 + EX31_RS15750 0.58 +2.6 2,351 + EX31_RS15750 0.59 +0.3 2,351 + EX31_RS15750 0.59 -1.0 2,351 + EX31_RS15750 0.59 +1.1 2,351 + EX31_RS15750 0.59 -0.5 2,351 + EX31_RS15750 0.59 +1.1 2,352 - EX31_RS15750 0.59 +1.1 2,353 + EX31_RS15750 0.59 +0.6 2,353 + EX31_RS15750 0.59 +2.2 2,354 - EX31_RS15750 0.60 -0.6 2,354 - EX31_RS15750 0.60 -0.3 2,354 - EX31_RS15750 0.60 +0.2 2,354 - EX31_RS15750 0.60 -0.1 2,453 + EX31_RS15750 0.73 +0.9 2,455 + EX31_RS15750 0.73 +0.8 2,458 - EX31_RS15750 0.74 +1.0 2,491 + EX31_RS15750 0.78 -0.1 2,493 + EX31_RS15750 0.79 -0.2 2,493 + EX31_RS15750 0.79 +0.7 2,493 + EX31_RS15750 0.79 +0.8 2,493 + EX31_RS15750 0.79 -0.1 2,494 - EX31_RS15750 0.79 -1.5 2,494 - EX31_RS15750 0.79 -1.2 2,494 - EX31_RS15750 0.79 +0.3 2,494 - EX31_RS15750 0.79 +0.7 2,496 + EX31_RS15750 0.79 -0.2 2,506 + EX31_RS15750 0.80 -0.6 2,507 - EX31_RS15750 0.81 -0.1 2,507 - EX31_RS15750 0.81 +0.5 2,535 + EX31_RS15750 0.84 +0.4 2,535 + EX31_RS15750 0.84 -0.9 2,535 + EX31_RS15750 0.84 +1.0 2,536 - EX31_RS15750 0.85 -0.3 2,536 - EX31_RS15750 0.85 +0.6 2,554 + EX31_RS15750 0.87 +0.4 2,572 + EX31_RS15750 0.90 +0.2 2,585 + +0.9 2,830 + -1.1 2,833 - -0.2 2,875 + +1.3 2,905 - -1.0
Or see this region's nucleotide sequence