Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09575 and EX31_RS09580 overlap by 8 nucleotides EX31_RS09580 and EX31_RS09585 overlap by 1 nucleotides EX31_RS09585 and EX31_RS09590 overlap by 4 nucleotides EX31_RS09590 and EX31_RS09595 are separated by 167 nucleotides EX31_RS09595 and EX31_RS09600 are separated by 3 nucleotides
EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513
_RS09575
EX31_RS09580: EX31_RS09580 - flagellar motor switch protein FliN, at 120,506 to 120,922
_RS09580
EX31_RS09585: EX31_RS09585 - flagellar biosynthetic protein FliO, at 120,922 to 121,359
_RS09585
EX31_RS09590: EX31_RS09590 - flagellar type III secretion system pore protein FliP, at 121,356 to 122,138
_RS09590
EX31_RS09595: EX31_RS09595 - flagellar biosynthesis protein FliQ, at 122,306 to 122,575
_RS09595
EX31_RS09600: EX31_RS09600 - flagellar type III secretion system protein FliR, at 122,579 to 123,364
_RS09600
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-1
0
1
2 at 120.431 kb on + strand at 120.431 kb on + strand at 120.442 kb on + strand at 120.488 kb on + strand at 120.488 kb on + strand at 120.489 kb on - strand at 120.520 kb on + strand at 120.722 kb on - strand, within EX31_RS09580 at 120.860 kb on + strand, within EX31_RS09580 at 120.861 kb on - strand, within EX31_RS09580 at 120.901 kb on + strand at 120.901 kb on + strand at 120.901 kb on + strand at 120.902 kb on - strand at 120.952 kb on + strand at 120.952 kb on + strand at 121.035 kb on + strand, within EX31_RS09585 at 121.148 kb on + strand, within EX31_RS09585 at 121.243 kb on - strand, within EX31_RS09585 at 121.435 kb on + strand, within EX31_RS09590 at 121.618 kb on + strand, within EX31_RS09590 at 121.725 kb on + strand, within EX31_RS09590 at 121.737 kb on + strand, within EX31_RS09590 at 121.921 kb on - strand, within EX31_RS09590 at 122.008 kb on + strand, within EX31_RS09590 at 122.008 kb on + strand, within EX31_RS09590 at 122.305 kb on + strand at 122.532 kb on - strand, within EX31_RS09595 at 122.549 kb on - strand at 122.561 kb on + strand at 122.562 kb on - strand at 122.562 kb on - strand at 122.562 kb on - strand at 122.562 kb on - strand at 122.624 kb on + strand at 122.624 kb on + strand at 122.625 kb on - strand at 122.728 kb on + strand, within EX31_RS09600 at 122.812 kb on + strand, within EX31_RS09600 at 122.918 kb on + strand, within EX31_RS09600 at 122.919 kb on - strand, within EX31_RS09600 at 123.114 kb on + strand, within EX31_RS09600
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 120,431 + +0.9 120,431 + +1.0 120,442 + +0.4 120,488 + +0.7 120,488 + -1.4 120,489 - +2.3 120,520 + +1.7 120,722 - EX31_RS09580 0.52 -1.1 120,860 + EX31_RS09580 0.85 +0.5 120,861 - EX31_RS09580 0.85 -0.3 120,901 + +1.2 120,901 + +1.6 120,901 + +1.3 120,902 - -0.3 120,952 + -0.3 120,952 + +0.8 121,035 + EX31_RS09585 0.26 +0.4 121,148 + EX31_RS09585 0.52 +0.9 121,243 - EX31_RS09585 0.73 +0.5 121,435 + EX31_RS09590 0.10 +1.7 121,618 + EX31_RS09590 0.33 +1.2 121,725 + EX31_RS09590 0.47 +1.7 121,737 + EX31_RS09590 0.49 +0.7 121,921 - EX31_RS09590 0.72 -0.6 122,008 + EX31_RS09590 0.83 +0.2 122,008 + EX31_RS09590 0.83 +1.2 122,305 + -0.9 122,532 - EX31_RS09595 0.84 +0.7 122,549 - +2.2 122,561 + +1.1 122,562 - +1.5 122,562 - +1.9 122,562 - +0.4 122,562 - -0.6 122,624 + +0.8 122,624 + +1.1 122,625 - +2.4 122,728 + EX31_RS09600 0.19 +1.8 122,812 + EX31_RS09600 0.30 -0.3 122,918 + EX31_RS09600 0.43 +0.7 122,919 - EX31_RS09600 0.43 -0.4 123,114 + EX31_RS09600 0.68 +1.1
Or see this region's nucleotide sequence