Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04410 and EX31_RS04415 are separated by 75 nucleotides EX31_RS04415 and EX31_RS04420 are separated by 116 nucleotides EX31_RS04420 and EX31_RS04425 are separated by 38 nucleotides
EX31_RS04410: EX31_RS04410 - type 1 fimbrial protein, at 413,488 to 414,015
_RS04410
EX31_RS04415: EX31_RS04415 - type 1 fimbrial protein, at 414,091 to 414,624
_RS04415
EX31_RS04420: EX31_RS04420 - molecular chaperone, at 414,741 to 415,415
_RS04420
EX31_RS04425: EX31_RS04425 - fimbrial biogenesis outer membrane usher protein, at 415,454 to 418,108
_RS04425
Position (kb) 414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 413.176 kb on + strand at 413.177 kb on - strand at 413.177 kb on - strand at 413.177 kb on - strand at 413.178 kb on + strand at 413.178 kb on + strand at 413.178 kb on + strand at 413.178 kb on + strand at 413.179 kb on - strand at 413.187 kb on - strand at 413.208 kb on - strand at 413.231 kb on - strand at 413.231 kb on - strand at 413.233 kb on - strand at 413.233 kb on - strand at 413.248 kb on - strand at 413.309 kb on + strand at 413.313 kb on + strand at 413.313 kb on + strand at 413.313 kb on + strand at 413.313 kb on + strand at 413.313 kb on + strand at 413.313 kb on + strand at 413.314 kb on - strand at 413.314 kb on - strand at 413.314 kb on - strand at 413.314 kb on - strand at 413.314 kb on - strand at 413.314 kb on - strand at 413.315 kb on + strand at 413.315 kb on + strand at 413.315 kb on + strand at 413.315 kb on + strand at 413.316 kb on - strand at 413.316 kb on - strand at 413.316 kb on - strand at 413.316 kb on - strand at 413.316 kb on - strand at 413.317 kb on + strand at 413.345 kb on + strand at 413.346 kb on - strand at 413.354 kb on + strand at 413.380 kb on + strand at 413.381 kb on - strand at 413.387 kb on - strand at 413.451 kb on - strand at 413.451 kb on - strand at 413.471 kb on - strand at 413.475 kb on + strand at 413.476 kb on - strand at 413.483 kb on + strand at 413.487 kb on - strand at 413.519 kb on + strand at 413.520 kb on - strand at 413.565 kb on + strand, within EX31_RS04410 at 413.571 kb on + strand, within EX31_RS04410 at 413.572 kb on - strand, within EX31_RS04410 at 413.583 kb on + strand, within EX31_RS04410 at 413.583 kb on + strand, within EX31_RS04410 at 413.589 kb on + strand, within EX31_RS04410 at 413.589 kb on + strand, within EX31_RS04410 at 413.589 kb on + strand, within EX31_RS04410 at 413.596 kb on - strand, within EX31_RS04410 at 413.596 kb on - strand, within EX31_RS04410 at 413.596 kb on - strand, within EX31_RS04410 at 413.747 kb on + strand, within EX31_RS04410 at 413.748 kb on - strand, within EX31_RS04410 at 413.782 kb on - strand, within EX31_RS04410 at 413.782 kb on - strand, within EX31_RS04410 at 413.805 kb on + strand, within EX31_RS04410 at 413.805 kb on + strand, within EX31_RS04410 at 413.805 kb on + strand, within EX31_RS04410 at 413.838 kb on + strand, within EX31_RS04410 at 413.838 kb on + strand, within EX31_RS04410 at 413.838 kb on + strand, within EX31_RS04410 at 413.838 kb on + strand, within EX31_RS04410 at 413.838 kb on + strand, within EX31_RS04410 at 413.839 kb on - strand, within EX31_RS04410 at 413.842 kb on + strand, within EX31_RS04410 at 413.842 kb on + strand, within EX31_RS04410 at 413.842 kb on + strand, within EX31_RS04410 at 413.843 kb on - strand, within EX31_RS04410 at 413.843 kb on - strand, within EX31_RS04410 at 413.844 kb on + strand, within EX31_RS04410 at 413.844 kb on + strand, within EX31_RS04410 at 413.991 kb on + strand at 413.992 kb on - strand at 413.992 kb on - strand at 413.992 kb on - strand at 414.007 kb on + strand at 414.007 kb on + strand at 414.007 kb on + strand at 414.007 kb on + strand at 414.007 kb on + strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.008 kb on - strand at 414.013 kb on + strand at 414.016 kb on + strand at 414.016 kb on + strand at 414.016 kb on + strand at 414.017 kb on - strand at 414.028 kb on + strand at 414.061 kb on + strand at 414.061 kb on + strand at 414.072 kb on + strand at 414.096 kb on + strand at 414.107 kb on + strand at 414.107 kb on + strand at 414.119 kb on + strand at 414.119 kb on + strand at 414.119 kb on + strand at 414.120 kb on - strand at 414.126 kb on + strand at 414.126 kb on + strand at 414.126 kb on + strand at 414.127 kb on - strand at 414.127 kb on - strand at 414.127 kb on - strand at 414.127 kb on - strand at 414.127 kb on - strand at 414.250 kb on - strand, within EX31_RS04415 at 414.250 kb on - strand, within EX31_RS04415 at 414.269 kb on + strand, within EX31_RS04415 at 414.269 kb on + strand, within EX31_RS04415 at 414.270 kb on - strand, within EX31_RS04415 at 414.276 kb on + strand, within EX31_RS04415 at 414.276 kb on + strand, within EX31_RS04415 at 414.277 kb on - strand, within EX31_RS04415 at 414.277 kb on - strand, within EX31_RS04415 at 414.277 kb on - strand, within EX31_RS04415 at 414.277 kb on - strand, within EX31_RS04415 at 414.318 kb on + strand, within EX31_RS04415 at 414.318 kb on + strand, within EX31_RS04415 at 414.318 kb on + strand, within EX31_RS04415 at 414.318 kb on + strand, within EX31_RS04415 at 414.318 kb on + strand, within EX31_RS04415 at 414.319 kb on - strand, within EX31_RS04415 at 414.319 kb on - strand, within EX31_RS04415 at 414.319 kb on - strand, within EX31_RS04415 at 414.319 kb on - strand, within EX31_RS04415 at 414.319 kb on - strand, within EX31_RS04415 at 414.430 kb on + strand, within EX31_RS04415 at 414.430 kb on + strand, within EX31_RS04415 at 414.430 kb on + strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.431 kb on - strand, within EX31_RS04415 at 414.439 kb on - strand, within EX31_RS04415 at 414.439 kb on - strand, within EX31_RS04415 at 414.471 kb on + strand, within EX31_RS04415 at 414.471 kb on + strand, within EX31_RS04415 at 414.472 kb on - strand, within EX31_RS04415 at 414.475 kb on - strand, within EX31_RS04415 at 414.475 kb on - strand, within EX31_RS04415 at 414.498 kb on + strand, within EX31_RS04415 at 414.498 kb on + strand, within EX31_RS04415 at 414.533 kb on - strand, within EX31_RS04415 at 414.533 kb on - strand, within EX31_RS04415 at 414.533 kb on - strand, within EX31_RS04415 at 414.582 kb on + strand at 414.582 kb on + strand at 414.582 kb on + strand at 414.582 kb on + strand at 414.582 kb on + strand at 414.583 kb on - strand at 414.583 kb on - strand at 414.585 kb on + strand at 414.585 kb on + strand at 414.585 kb on + strand at 414.586 kb on - strand at 414.586 kb on - strand at 414.586 kb on - strand at 414.599 kb on + strand at 414.600 kb on - strand at 414.609 kb on + strand at 414.609 kb on + strand at 414.609 kb on + strand at 414.609 kb on + strand at 414.609 kb on + strand at 414.609 kb on + strand at 414.610 kb on - strand at 414.610 kb on - strand at 414.610 kb on - strand at 414.610 kb on - strand at 414.610 kb on - strand at 414.626 kb on - strand at 414.635 kb on - strand at 414.635 kb on - strand at 414.638 kb on - strand at 414.638 kb on - strand at 414.655 kb on - strand at 414.688 kb on + strand at 414.704 kb on - strand at 414.775 kb on + strand at 414.898 kb on - strand, within EX31_RS04420 at 414.951 kb on - strand, within EX31_RS04420 at 415.000 kb on + strand, within EX31_RS04420 at 415.000 kb on + strand, within EX31_RS04420 at 415.001 kb on - strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.004 kb on + strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.005 kb on - strand, within EX31_RS04420 at 415.050 kb on + strand, within EX31_RS04420 at 415.050 kb on + strand, within EX31_RS04420 at 415.058 kb on + strand, within EX31_RS04420 at 415.059 kb on - strand, within EX31_RS04420 at 415.070 kb on + strand, within EX31_RS04420 at 415.112 kb on + strand, within EX31_RS04420 at 415.118 kb on + strand, within EX31_RS04420 at 415.118 kb on + strand, within EX31_RS04420 at 415.119 kb on - strand, within EX31_RS04420 at 415.124 kb on + strand, within EX31_RS04420 at 415.124 kb on + strand, within EX31_RS04420 at 415.124 kb on + strand, within EX31_RS04420 at 415.125 kb on - strand, within EX31_RS04420 at 415.125 kb on - strand, within EX31_RS04420 at 415.125 kb on - strand, within EX31_RS04420 at 415.138 kb on - strand, within EX31_RS04420 at 415.158 kb on - strand, within EX31_RS04420 at 415.242 kb on + strand, within EX31_RS04420 at 415.242 kb on + strand, within EX31_RS04420 at 415.242 kb on + strand, within EX31_RS04420 at 415.242 kb on + strand, within EX31_RS04420 at 415.242 kb on + strand, within EX31_RS04420 at 415.243 kb on - strand, within EX31_RS04420 at 415.243 kb on - strand, within EX31_RS04420 at 415.245 kb on + strand, within EX31_RS04420 at 415.245 kb on + strand, within EX31_RS04420 at 415.246 kb on - strand, within EX31_RS04420 at 415.246 kb on - strand, within EX31_RS04420 at 415.296 kb on + strand, within EX31_RS04420 at 415.296 kb on + strand, within EX31_RS04420 at 415.296 kb on + strand, within EX31_RS04420 at 415.296 kb on + strand, within EX31_RS04420 at 415.297 kb on - strand, within EX31_RS04420 at 415.297 kb on - strand, within EX31_RS04420 at 415.299 kb on - strand, within EX31_RS04420 at 415.299 kb on - strand, within EX31_RS04420 at 415.299 kb on - strand, within EX31_RS04420 at 415.371 kb on - strand at 415.417 kb on + strand at 415.472 kb on - strand at 415.546 kb on + strand at 415.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 413,176 + -0.5 413,177 - -0.1 413,177 - +0.1 413,177 - -0.5 413,178 + -1.6 413,178 + +1.3 413,178 + -0.7 413,178 + -1.8 413,179 - +0.3 413,187 - -1.2 413,208 - +0.1 413,231 - +0.0 413,231 - +0.3 413,233 - -1.7 413,233 - +1.1 413,248 - -0.0 413,309 + +0.4 413,313 + +0.0 413,313 + -0.9 413,313 + +0.8 413,313 + -1.1 413,313 + +0.7 413,313 + -1.7 413,314 - -0.2 413,314 - +1.8 413,314 - +1.3 413,314 - +1.0 413,314 - +0.1 413,314 - -1.4 413,315 + -1.1 413,315 + +0.4 413,315 + -2.0 413,315 + -1.7 413,316 - -0.0 413,316 - +0.5 413,316 - +1.2 413,316 - +1.1 413,316 - +0.2 413,317 + -1.0 413,345 + +1.0 413,346 - -0.2 413,354 + +0.4 413,380 + +1.1 413,381 - +0.9 413,387 - +0.1 413,451 - +1.2 413,451 - +0.2 413,471 - +0.5 413,475 + -1.0 413,476 - +0.3 413,483 + +0.0 413,487 - -1.7 413,519 + +2.2 413,520 - +2.1 413,565 + EX31_RS04410 0.15 +0.1 413,571 + EX31_RS04410 0.16 +0.7 413,572 - EX31_RS04410 0.16 -0.3 413,583 + EX31_RS04410 0.18 +0.1 413,583 + EX31_RS04410 0.18 -0.5 413,589 + EX31_RS04410 0.19 +0.1 413,589 + EX31_RS04410 0.19 -0.5 413,589 + EX31_RS04410 0.19 +1.3 413,596 - EX31_RS04410 0.20 +0.0 413,596 - EX31_RS04410 0.20 +0.1 413,596 - EX31_RS04410 0.20 -0.2 413,747 + EX31_RS04410 0.49 -0.4 413,748 - EX31_RS04410 0.49 -1.3 413,782 - EX31_RS04410 0.56 -0.4 413,782 - EX31_RS04410 0.56 -0.2 413,805 + EX31_RS04410 0.60 -0.1 413,805 + EX31_RS04410 0.60 -0.4 413,805 + EX31_RS04410 0.60 -0.7 413,838 + EX31_RS04410 0.66 -0.2 413,838 + EX31_RS04410 0.66 -0.7 413,838 + EX31_RS04410 0.66 +0.7 413,838 + EX31_RS04410 0.66 +0.4 413,838 + EX31_RS04410 0.66 -0.3 413,839 - EX31_RS04410 0.66 -0.7 413,842 + EX31_RS04410 0.67 +0.1 413,842 + EX31_RS04410 0.67 +0.5 413,842 + EX31_RS04410 0.67 +1.4 413,843 - EX31_RS04410 0.67 +1.0 413,843 - EX31_RS04410 0.67 +0.0 413,844 + EX31_RS04410 0.67 +2.1 413,844 + EX31_RS04410 0.67 +0.2 413,991 + +0.1 413,992 - -0.2 413,992 - +0.7 413,992 - +0.5 414,007 + +0.5 414,007 + -0.0 414,007 + +0.3 414,007 + -0.4 414,007 + +0.5 414,008 - -0.5 414,008 - +0.7 414,008 - +0.8 414,008 - +1.2 414,008 - -0.1 414,008 - -0.9 414,008 - -0.4 414,013 + -0.2 414,016 + -0.1 414,016 + -1.5 414,016 + -0.3 414,017 - +0.6 414,028 + -0.1 414,061 + -1.0 414,061 + -0.1 414,072 + -0.4 414,096 + +0.8 414,107 + +0.4 414,107 + -0.4 414,119 + -1.0 414,119 + -0.3 414,119 + +0.3 414,120 - -0.0 414,126 + -1.2 414,126 + +1.6 414,126 + +0.5 414,127 - -0.7 414,127 - +0.5 414,127 - +0.6 414,127 - -0.1 414,127 - +0.8 414,250 - EX31_RS04415 0.30 -0.7 414,250 - EX31_RS04415 0.30 -0.5 414,269 + EX31_RS04415 0.33 +0.7 414,269 + EX31_RS04415 0.33 -0.2 414,270 - EX31_RS04415 0.34 +0.6 414,276 + EX31_RS04415 0.35 +0.1 414,276 + EX31_RS04415 0.35 -1.1 414,277 - EX31_RS04415 0.35 -0.3 414,277 - EX31_RS04415 0.35 -0.9 414,277 - EX31_RS04415 0.35 -0.1 414,277 - EX31_RS04415 0.35 -1.5 414,318 + EX31_RS04415 0.43 -0.8 414,318 + EX31_RS04415 0.43 +0.6 414,318 + EX31_RS04415 0.43 -1.7 414,318 + EX31_RS04415 0.43 +0.7 414,318 + EX31_RS04415 0.43 -0.4 414,319 - EX31_RS04415 0.43 -0.4 414,319 - EX31_RS04415 0.43 +0.3 414,319 - EX31_RS04415 0.43 +1.8 414,319 - EX31_RS04415 0.43 +1.1 414,319 - EX31_RS04415 0.43 +2.4 414,430 + EX31_RS04415 0.63 -0.9 414,430 + EX31_RS04415 0.63 +0.8 414,430 + EX31_RS04415 0.63 +0.4 414,431 - EX31_RS04415 0.64 -1.2 414,431 - EX31_RS04415 0.64 +0.8 414,431 - EX31_RS04415 0.64 -2.2 414,431 - EX31_RS04415 0.64 -0.9 414,431 - EX31_RS04415 0.64 -0.0 414,431 - EX31_RS04415 0.64 -1.0 414,431 - EX31_RS04415 0.64 +1.5 414,439 - EX31_RS04415 0.65 +0.4 414,439 - EX31_RS04415 0.65 -0.3 414,471 + EX31_RS04415 0.71 -1.5 414,471 + EX31_RS04415 0.71 -1.1 414,472 - EX31_RS04415 0.71 -0.0 414,475 - EX31_RS04415 0.72 +2.6 414,475 - EX31_RS04415 0.72 -0.3 414,498 + EX31_RS04415 0.76 +0.7 414,498 + EX31_RS04415 0.76 +0.2 414,533 - EX31_RS04415 0.83 +0.1 414,533 - EX31_RS04415 0.83 -0.8 414,533 - EX31_RS04415 0.83 -1.0 414,582 + +0.7 414,582 + +0.4 414,582 + -0.3 414,582 + -1.0 414,582 + +2.1 414,583 - +0.3 414,583 - +1.2 414,585 + -0.6 414,585 + -0.1 414,585 + +1.3 414,586 - +0.8 414,586 - -0.9 414,586 - -0.3 414,599 + +0.7 414,600 - -0.2 414,609 + -0.2 414,609 + -0.2 414,609 + +0.1 414,609 + +0.3 414,609 + +0.3 414,609 + -0.1 414,610 - -0.6 414,610 - +1.6 414,610 - -1.3 414,610 - -1.1 414,610 - -0.9 414,626 - -0.5 414,635 - -0.1 414,635 - +1.3 414,638 - +1.0 414,638 - +0.4 414,655 - -2.1 414,688 + -0.2 414,704 - -3.4 414,775 + +1.4 414,898 - EX31_RS04420 0.23 +0.3 414,951 - EX31_RS04420 0.31 -1.0 415,000 + EX31_RS04420 0.38 -0.8 415,000 + EX31_RS04420 0.38 +0.5 415,001 - EX31_RS04420 0.39 +0.6 415,004 + EX31_RS04420 0.39 +0.4 415,004 + EX31_RS04420 0.39 +1.4 415,004 + EX31_RS04420 0.39 -1.7 415,004 + EX31_RS04420 0.39 +2.4 415,004 + EX31_RS04420 0.39 +0.3 415,004 + EX31_RS04420 0.39 -1.0 415,005 - EX31_RS04420 0.39 +1.1 415,005 - EX31_RS04420 0.39 +0.4 415,005 - EX31_RS04420 0.39 +1.6 415,005 - EX31_RS04420 0.39 +1.0 415,005 - EX31_RS04420 0.39 -0.3 415,005 - EX31_RS04420 0.39 -1.3 415,005 - EX31_RS04420 0.39 -1.2 415,050 + EX31_RS04420 0.46 -0.1 415,050 + EX31_RS04420 0.46 -0.6 415,058 + EX31_RS04420 0.47 -0.4 415,059 - EX31_RS04420 0.47 +0.6 415,070 + EX31_RS04420 0.49 +0.7 415,112 + EX31_RS04420 0.55 +1.3 415,118 + EX31_RS04420 0.56 +0.3 415,118 + EX31_RS04420 0.56 +0.2 415,119 - EX31_RS04420 0.56 -1.5 415,124 + EX31_RS04420 0.57 +1.1 415,124 + EX31_RS04420 0.57 +0.2 415,124 + EX31_RS04420 0.57 +1.4 415,125 - EX31_RS04420 0.57 -0.7 415,125 - EX31_RS04420 0.57 +2.4 415,125 - EX31_RS04420 0.57 +0.0 415,138 - EX31_RS04420 0.59 +0.5 415,158 - EX31_RS04420 0.62 +1.4 415,242 + EX31_RS04420 0.74 +0.8 415,242 + EX31_RS04420 0.74 -0.0 415,242 + EX31_RS04420 0.74 -0.0 415,242 + EX31_RS04420 0.74 +0.7 415,242 + EX31_RS04420 0.74 -0.1 415,243 - EX31_RS04420 0.74 -0.1 415,243 - EX31_RS04420 0.74 -1.2 415,245 + EX31_RS04420 0.75 -1.2 415,245 + EX31_RS04420 0.75 +1.2 415,246 - EX31_RS04420 0.75 +0.9 415,246 - EX31_RS04420 0.75 -2.1 415,296 + EX31_RS04420 0.82 -0.0 415,296 + EX31_RS04420 0.82 -0.4 415,296 + EX31_RS04420 0.82 -1.5 415,296 + EX31_RS04420 0.82 -1.5 415,297 - EX31_RS04420 0.82 -0.9 415,297 - EX31_RS04420 0.82 -1.3 415,299 - EX31_RS04420 0.83 -0.6 415,299 - EX31_RS04420 0.83 -1.0 415,299 - EX31_RS04420 0.83 -0.5 415,371 - -1.0 415,417 + +0.4 415,472 - +0.0 415,546 + -0.1 415,594 - +0.4
Or see this region's nucleotide sequence