Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04000 and EX31_RS04005 are separated by 64 nucleotides EX31_RS04005 and EX31_RS04010 are separated by 100 nucleotides
EX31_RS04000: EX31_RS04000 - energy-dependent translational throttle protein EttA, at 319,864 to 321,531
_RS04000
EX31_RS04005: EX31_RS04005 - LysR family transcriptional regulator, at 321,596 to 322,504
_RS04005
EX31_RS04010: EX31_RS04010 - zinc-binding alcohol dehydrogenase family protein, at 322,605 to 323,606
_RS04010
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.661 kb on + strand, within EX31_RS04000 at 320.661 kb on + strand, within EX31_RS04000 at 320.661 kb on + strand, within EX31_RS04000 at 320.662 kb on - strand, within EX31_RS04000 at 320.668 kb on - strand, within EX31_RS04000 at 320.668 kb on - strand, within EX31_RS04000 at 320.668 kb on - strand, within EX31_RS04000 at 320.718 kb on + strand, within EX31_RS04000 at 320.860 kb on + strand, within EX31_RS04000 at 320.860 kb on + strand, within EX31_RS04000 at 320.861 kb on - strand, within EX31_RS04000 at 320.879 kb on + strand, within EX31_RS04000 at 320.965 kb on - strand, within EX31_RS04000 at 321.142 kb on + strand, within EX31_RS04000 at 321.142 kb on + strand, within EX31_RS04000 at 321.142 kb on + strand, within EX31_RS04000 at 321.142 kb on + strand, within EX31_RS04000 at 321.143 kb on - strand, within EX31_RS04000 at 321.404 kb on - strand at 321.404 kb on - strand at 321.417 kb on + strand at 321.438 kb on + strand at 321.438 kb on + strand at 321.438 kb on + strand at 321.438 kb on + strand at 321.439 kb on - strand at 321.439 kb on - strand at 321.439 kb on - strand at 321.461 kb on - strand at 321.461 kb on - strand at 321.461 kb on - strand at 321.471 kb on + strand at 321.471 kb on + strand at 321.472 kb on - strand at 321.476 kb on + strand at 321.476 kb on + strand at 321.477 kb on - strand at 321.477 kb on - strand at 321.529 kb on + strand at 321.529 kb on + strand at 321.530 kb on - strand at 321.530 kb on - strand at 321.548 kb on + strand at 321.548 kb on + strand at 321.548 kb on + strand at 321.548 kb on + strand at 321.549 kb on - strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.550 kb on + strand at 321.551 kb on - strand at 321.551 kb on - strand at 321.551 kb on - strand at 321.551 kb on - strand at 321.551 kb on - strand at 321.551 kb on - strand at 321.574 kb on + strand at 321.618 kb on + strand at 321.618 kb on + strand at 321.650 kb on - strand at 321.650 kb on - strand at 321.659 kb on - strand at 321.708 kb on + strand, within EX31_RS04005 at 321.709 kb on - strand, within EX31_RS04005 at 321.709 kb on - strand, within EX31_RS04005 at 321.806 kb on - strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.837 kb on + strand, within EX31_RS04005 at 321.838 kb on - strand, within EX31_RS04005 at 321.838 kb on - strand, within EX31_RS04005 at 321.838 kb on - strand, within EX31_RS04005 at 321.838 kb on - strand, within EX31_RS04005 at 321.856 kb on + strand, within EX31_RS04005 at 321.857 kb on - strand, within EX31_RS04005 at 321.857 kb on - strand, within EX31_RS04005 at 321.902 kb on + strand, within EX31_RS04005 at 321.902 kb on + strand, within EX31_RS04005 at 321.903 kb on - strand, within EX31_RS04005 at 321.903 kb on - strand, within EX31_RS04005 at 321.903 kb on - strand, within EX31_RS04005 at 321.903 kb on - strand, within EX31_RS04005 at 321.934 kb on + strand, within EX31_RS04005 at 321.990 kb on + strand, within EX31_RS04005 at 322.064 kb on - strand, within EX31_RS04005 at 322.245 kb on - strand, within EX31_RS04005 at 322.245 kb on - strand, within EX31_RS04005 at 322.308 kb on + strand, within EX31_RS04005 at 322.309 kb on - strand, within EX31_RS04005 at 322.333 kb on + strand, within EX31_RS04005 at 322.333 kb on + strand, within EX31_RS04005 at 322.333 kb on + strand, within EX31_RS04005 at 322.334 kb on - strand, within EX31_RS04005 at 322.334 kb on - strand, within EX31_RS04005 at 322.334 kb on - strand, within EX31_RS04005 at 322.334 kb on - strand, within EX31_RS04005 at 322.498 kb on + strand at 322.499 kb on - strand at 322.499 kb on - strand at 322.537 kb on + strand at 322.537 kb on + strand at 322.538 kb on - strand at 322.550 kb on - strand at 322.571 kb on + strand at 322.571 kb on + strand at 322.571 kb on + strand at 322.572 kb on - strand at 322.572 kb on - strand at 322.572 kb on - strand at 322.572 kb on - strand at 322.572 kb on - strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.582 kb on + strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.583 kb on - strand at 322.584 kb on + strand at 322.584 kb on + strand at 322.584 kb on + strand at 322.584 kb on + strand at 322.584 kb on + strand at 322.584 kb on + strand at 322.585 kb on - strand at 322.619 kb on + strand at 322.619 kb on + strand at 322.620 kb on - strand at 322.620 kb on - strand at 322.652 kb on + strand at 322.653 kb on - strand at 322.653 kb on - strand at 322.653 kb on - strand at 322.653 kb on - strand at 322.690 kb on - strand at 322.690 kb on - strand at 322.748 kb on + strand, within EX31_RS04010 at 322.748 kb on + strand, within EX31_RS04010 at 322.748 kb on + strand, within EX31_RS04010 at 322.749 kb on - strand, within EX31_RS04010 at 322.867 kb on - strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.925 kb on + strand, within EX31_RS04010 at 322.926 kb on - strand, within EX31_RS04010 at 322.926 kb on - strand, within EX31_RS04010 at 322.926 kb on - strand, within EX31_RS04010 at 322.926 kb on - strand, within EX31_RS04010 at 322.926 kb on - strand, within EX31_RS04010 at 323.129 kb on + strand, within EX31_RS04010 at 323.129 kb on + strand, within EX31_RS04010 at 323.199 kb on + strand, within EX31_RS04010 at 323.199 kb on + strand, within EX31_RS04010 at 323.199 kb on + strand, within EX31_RS04010 at 323.201 kb on + strand, within EX31_RS04010 at 323.202 kb on - strand, within EX31_RS04010 at 323.202 kb on - strand, within EX31_RS04010 at 323.327 kb on + strand, within EX31_RS04010 at 323.327 kb on + strand, within EX31_RS04010 at 323.327 kb on + strand, within EX31_RS04010 at 323.358 kb on - strand, within EX31_RS04010 at 323.363 kb on + strand, within EX31_RS04010 at 323.364 kb on - strand, within EX31_RS04010 at 323.406 kb on + strand, within EX31_RS04010 at 323.406 kb on + strand, within EX31_RS04010 at 323.406 kb on + strand, within EX31_RS04010 at 323.406 kb on + strand, within EX31_RS04010 at 323.406 kb on + strand, within EX31_RS04010 at 323.407 kb on - strand, within EX31_RS04010 at 323.407 kb on - strand, within EX31_RS04010 at 323.407 kb on - strand, within EX31_RS04010 at 323.423 kb on + strand, within EX31_RS04010 at 323.423 kb on + strand, within EX31_RS04010 at 323.423 kb on + strand, within EX31_RS04010 at 323.423 kb on + strand, within EX31_RS04010 at 323.424 kb on - strand, within EX31_RS04010 at 323.424 kb on - strand, within EX31_RS04010 at 323.494 kb on + strand, within EX31_RS04010 at 323.495 kb on - strand, within EX31_RS04010
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 320,661 + EX31_RS04000 0.48 -0.2 320,661 + EX31_RS04000 0.48 +0.9 320,661 + EX31_RS04000 0.48 -0.9 320,662 - EX31_RS04000 0.48 +1.2 320,668 - EX31_RS04000 0.48 +0.3 320,668 - EX31_RS04000 0.48 +0.6 320,668 - EX31_RS04000 0.48 +0.6 320,718 + EX31_RS04000 0.51 +0.1 320,860 + EX31_RS04000 0.60 -0.4 320,860 + EX31_RS04000 0.60 +0.5 320,861 - EX31_RS04000 0.60 +1.3 320,879 + EX31_RS04000 0.61 +0.7 320,965 - EX31_RS04000 0.66 +0.0 321,142 + EX31_RS04000 0.77 -1.4 321,142 + EX31_RS04000 0.77 -0.3 321,142 + EX31_RS04000 0.77 -1.2 321,142 + EX31_RS04000 0.77 +0.4 321,143 - EX31_RS04000 0.77 +0.2 321,404 - -0.6 321,404 - -0.2 321,417 + +0.6 321,438 + -0.0 321,438 + -0.2 321,438 + +2.4 321,438 + -0.2 321,439 - -0.3 321,439 - +1.1 321,439 - +0.4 321,461 - +1.0 321,461 - -0.0 321,461 - -1.3 321,471 + +0.5 321,471 + -0.7 321,472 - +0.8 321,476 + +0.1 321,476 + +0.4 321,477 - +0.1 321,477 - -1.8 321,529 + +0.3 321,529 + +0.4 321,530 - +0.6 321,530 - -0.3 321,548 + -0.0 321,548 + +0.2 321,548 + -1.2 321,548 + -1.2 321,549 - +0.8 321,550 + +0.5 321,550 + -0.1 321,550 + +0.2 321,550 + +0.5 321,550 + -0.8 321,550 + -0.6 321,550 + -0.2 321,550 + -0.7 321,550 + +0.5 321,550 + -0.6 321,551 - +0.8 321,551 - +0.3 321,551 - -0.7 321,551 - +0.3 321,551 - +0.0 321,551 - -0.8 321,574 + -1.6 321,618 + -0.6 321,618 + +0.2 321,650 - -0.5 321,650 - +0.6 321,659 - +0.4 321,708 + EX31_RS04005 0.12 +0.4 321,709 - EX31_RS04005 0.12 +1.1 321,709 - EX31_RS04005 0.12 -0.4 321,806 - EX31_RS04005 0.23 +2.4 321,837 + EX31_RS04005 0.27 -0.3 321,837 + EX31_RS04005 0.27 -0.4 321,837 + EX31_RS04005 0.27 +0.5 321,837 + EX31_RS04005 0.27 +0.8 321,837 + EX31_RS04005 0.27 -0.7 321,837 + EX31_RS04005 0.27 +0.6 321,837 + EX31_RS04005 0.27 -2.7 321,838 - EX31_RS04005 0.27 -0.8 321,838 - EX31_RS04005 0.27 -0.1 321,838 - EX31_RS04005 0.27 -0.5 321,838 - EX31_RS04005 0.27 -0.3 321,856 + EX31_RS04005 0.29 -0.1 321,857 - EX31_RS04005 0.29 -0.5 321,857 - EX31_RS04005 0.29 -0.1 321,902 + EX31_RS04005 0.34 -0.2 321,902 + EX31_RS04005 0.34 +0.7 321,903 - EX31_RS04005 0.34 -0.3 321,903 - EX31_RS04005 0.34 +1.0 321,903 - EX31_RS04005 0.34 -1.0 321,903 - EX31_RS04005 0.34 +0.1 321,934 + EX31_RS04005 0.37 -0.4 321,990 + EX31_RS04005 0.43 +1.8 322,064 - EX31_RS04005 0.51 +1.0 322,245 - EX31_RS04005 0.71 -0.2 322,245 - EX31_RS04005 0.71 +0.4 322,308 + EX31_RS04005 0.78 +0.6 322,309 - EX31_RS04005 0.78 -0.2 322,333 + EX31_RS04005 0.81 -0.4 322,333 + EX31_RS04005 0.81 +0.2 322,333 + EX31_RS04005 0.81 -1.1 322,334 - EX31_RS04005 0.81 +0.5 322,334 - EX31_RS04005 0.81 -0.4 322,334 - EX31_RS04005 0.81 -2.0 322,334 - EX31_RS04005 0.81 +0.6 322,498 + +0.3 322,499 - -0.5 322,499 - -0.0 322,537 + -0.9 322,537 + +0.1 322,538 - +0.7 322,550 - -0.3 322,571 + -0.9 322,571 + -1.5 322,571 + +0.3 322,572 - +0.2 322,572 - +0.4 322,572 - +1.2 322,572 - -1.0 322,572 - -0.3 322,582 + -0.5 322,582 + +0.5 322,582 + +1.2 322,582 + -1.0 322,582 + -0.7 322,582 + +0.6 322,582 + -0.2 322,583 - -0.5 322,583 - -0.7 322,583 - -0.3 322,583 - -1.4 322,583 - -0.3 322,583 - +0.5 322,583 - -1.1 322,583 - +0.0 322,584 + -0.6 322,584 + +0.7 322,584 + -0.7 322,584 + +1.5 322,584 + -0.3 322,584 + -0.2 322,585 - +0.4 322,619 + -1.3 322,619 + +0.0 322,620 - -1.2 322,620 - +0.4 322,652 + -0.4 322,653 - -1.3 322,653 - +1.1 322,653 - +1.4 322,653 - +0.7 322,690 - -1.0 322,690 - -0.3 322,748 + EX31_RS04010 0.14 -1.4 322,748 + EX31_RS04010 0.14 +1.0 322,748 + EX31_RS04010 0.14 +0.6 322,749 - EX31_RS04010 0.14 +0.5 322,867 - EX31_RS04010 0.26 -0.8 322,925 + EX31_RS04010 0.32 -0.4 322,925 + EX31_RS04010 0.32 +0.3 322,925 + EX31_RS04010 0.32 -0.2 322,925 + EX31_RS04010 0.32 +0.4 322,925 + EX31_RS04010 0.32 -0.3 322,925 + EX31_RS04010 0.32 -0.2 322,925 + EX31_RS04010 0.32 -0.2 322,925 + EX31_RS04010 0.32 -0.2 322,926 - EX31_RS04010 0.32 -0.6 322,926 - EX31_RS04010 0.32 -1.0 322,926 - EX31_RS04010 0.32 -1.7 322,926 - EX31_RS04010 0.32 -1.2 322,926 - EX31_RS04010 0.32 +0.6 323,129 + EX31_RS04010 0.52 +1.1 323,129 + EX31_RS04010 0.52 -0.6 323,199 + EX31_RS04010 0.59 +0.7 323,199 + EX31_RS04010 0.59 -0.5 323,199 + EX31_RS04010 0.59 +0.6 323,201 + EX31_RS04010 0.59 -0.2 323,202 - EX31_RS04010 0.60 +0.8 323,202 - EX31_RS04010 0.60 +0.7 323,327 + EX31_RS04010 0.72 +0.3 323,327 + EX31_RS04010 0.72 -0.4 323,327 + EX31_RS04010 0.72 +0.4 323,358 - EX31_RS04010 0.75 -1.1 323,363 + EX31_RS04010 0.76 -0.9 323,364 - EX31_RS04010 0.76 -0.3 323,406 + EX31_RS04010 0.80 +0.7 323,406 + EX31_RS04010 0.80 -0.7 323,406 + EX31_RS04010 0.80 +0.2 323,406 + EX31_RS04010 0.80 -2.2 323,406 + EX31_RS04010 0.80 -0.5 323,407 - EX31_RS04010 0.80 +0.2 323,407 - EX31_RS04010 0.80 +0.7 323,407 - EX31_RS04010 0.80 +0.3 323,423 + EX31_RS04010 0.82 -0.4 323,423 + EX31_RS04010 0.82 -0.6 323,423 + EX31_RS04010 0.82 +0.7 323,423 + EX31_RS04010 0.82 +0.1 323,424 - EX31_RS04010 0.82 +0.3 323,424 - EX31_RS04010 0.82 +0.7 323,494 + EX31_RS04010 0.89 -0.0 323,495 - EX31_RS04010 0.89 +0.1
Or see this region's nucleotide sequence