Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25375 and CA265_RS25380 are separated by 29 nucleotides CA265_RS25380 and CA265_RS25385 overlap by 19 nucleotides CA265_RS25385 and CA265_RS25390 are separated by 16 nucleotides CA265_RS25390 and CA265_RS25395 are separated by 31 nucleotides CA265_RS25395 and CA265_RS25400 are separated by 81 nucleotides
CA265_RS25375: CA265_RS25375 - hypothetical protein, at 6,166,125 to 6,166,799
_RS25375
CA265_RS25380: CA265_RS25380 - hypothetical protein, at 6,166,829 to 6,167,038
_RS25380
CA265_RS25385: CA265_RS25385 - Txe/YoeB family addiction module toxin, at 6,167,020 to 6,167,301
_RS25385
CA265_RS25390: CA265_RS25390 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 6,167,318 to 6,168,169
_RS25390
CA265_RS25395: CA265_RS25395 - cysteine hydrolase, at 6,168,201 to 6,168,743
_RS25395
CA265_RS25400: CA265_RS25400 - fatty acid desaturase, at 6,168,825 to 6,169,451
_RS25400
Position (kb)
6167
6168
6169 Strain fitness (log2 ratio)
-1
0
1
2 at 6166.324 kb on + strand, within CA265_RS25375 at 6166.342 kb on + strand, within CA265_RS25375 at 6166.400 kb on + strand, within CA265_RS25375 at 6166.408 kb on - strand, within CA265_RS25375 at 6166.411 kb on - strand, within CA265_RS25375 at 6166.454 kb on - strand, within CA265_RS25375 at 6166.553 kb on + strand, within CA265_RS25375 at 6166.562 kb on - strand, within CA265_RS25375 at 6166.687 kb on - strand, within CA265_RS25375 at 6166.716 kb on + strand, within CA265_RS25375 at 6166.717 kb on - strand, within CA265_RS25375 at 6166.748 kb on - strand at 6166.748 kb on - strand at 6166.766 kb on - strand at 6167.036 kb on + strand at 6167.055 kb on + strand, within CA265_RS25385 at 6167.091 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.233 kb on + strand, within CA265_RS25385 at 6167.256 kb on + strand, within CA265_RS25385 at 6168.186 kb on - strand at 6168.188 kb on - strand at 6168.191 kb on + strand at 6168.220 kb on + strand at 6168.290 kb on - strand, within CA265_RS25395 at 6168.294 kb on - strand, within CA265_RS25395 at 6168.450 kb on - strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.579 kb on + strand, within CA265_RS25395 at 6168.631 kb on + strand, within CA265_RS25395 at 6168.646 kb on + strand, within CA265_RS25395 at 6168.710 kb on - strand at 6168.717 kb on - strand at 6168.742 kb on - strand at 6168.786 kb on - strand at 6168.786 kb on - strand at 6168.787 kb on + strand at 6168.795 kb on + strand at 6168.795 kb on + strand at 6168.796 kb on - strand at 6168.818 kb on - strand at 6168.824 kb on - strand at 6168.861 kb on - strand at 6168.861 kb on - strand at 6168.894 kb on + strand, within CA265_RS25400 at 6169.081 kb on + strand, within CA265_RS25400 at 6169.132 kb on - strand, within CA265_RS25400 at 6169.153 kb on - strand, within CA265_RS25400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 6,166,324 + CA265_RS25375 0.29 +0.7 6,166,342 + CA265_RS25375 0.32 +0.3 6,166,400 + CA265_RS25375 0.41 +0.3 6,166,408 - CA265_RS25375 0.42 +0.4 6,166,411 - CA265_RS25375 0.42 +0.2 6,166,454 - CA265_RS25375 0.49 +1.3 6,166,553 + CA265_RS25375 0.63 -0.7 6,166,562 - CA265_RS25375 0.65 +0.1 6,166,687 - CA265_RS25375 0.83 -0.0 6,166,716 + CA265_RS25375 0.88 -1.5 6,166,717 - CA265_RS25375 0.88 +2.0 6,166,748 - +0.1 6,166,748 - +0.0 6,166,766 - +0.2 6,167,036 + +0.5 6,167,055 + CA265_RS25385 0.12 -0.7 6,167,091 + CA265_RS25385 0.25 +0.1 6,167,132 + CA265_RS25385 0.40 +0.4 6,167,132 + CA265_RS25385 0.40 -0.2 6,167,231 + CA265_RS25385 0.75 +0.5 6,167,231 + CA265_RS25385 0.75 -0.9 6,167,233 + CA265_RS25385 0.76 -0.7 6,167,256 + CA265_RS25385 0.84 +1.0 6,168,186 - +1.5 6,168,188 - -0.0 6,168,191 + -0.2 6,168,220 + +0.6 6,168,290 - CA265_RS25395 0.16 +0.7 6,168,294 - CA265_RS25395 0.17 -0.0 6,168,450 - CA265_RS25395 0.46 +1.0 6,168,484 + CA265_RS25395 0.52 +1.7 6,168,484 + CA265_RS25395 0.52 -0.2 6,168,579 + CA265_RS25395 0.70 +2.0 6,168,631 + CA265_RS25395 0.79 -0.5 6,168,646 + CA265_RS25395 0.82 -0.5 6,168,710 - -0.2 6,168,717 - +0.9 6,168,742 - +0.1 6,168,786 - +2.3 6,168,786 - -0.6 6,168,787 + +0.9 6,168,795 + +1.6 6,168,795 + -1.1 6,168,796 - +1.1 6,168,818 - -0.8 6,168,824 - +1.0 6,168,861 - -0.2 6,168,861 - +1.2 6,168,894 + CA265_RS25400 0.11 -0.1 6,169,081 + CA265_RS25400 0.41 -0.5 6,169,132 - CA265_RS25400 0.49 -0.8 6,169,153 - CA265_RS25400 0.52 -0.2
Or see this region's nucleotide sequence