Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06305 and CA265_RS06310 are separated by 80 nucleotides CA265_RS06310 and CA265_RS06315 are separated by 137 nucleotides CA265_RS06315 and CA265_RS06320 are separated by 2 nucleotides
CA265_RS06305: CA265_RS06305 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,549,306 to 1,550,010
_RS06305
CA265_RS06310: CA265_RS06310 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,550,091 to 1,550,750
_RS06310
CA265_RS06315: CA265_RS06315 - uridine kinase, at 1,550,888 to 1,551,532
_RS06315
CA265_RS06320: CA265_RS06320 - hypothetical protein, at 1,551,535 to 1,553,073
_RS06320
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-2
-1
0
1 at 1549.914 kb on + strand, within CA265_RS06305 at 1549.915 kb on - strand, within CA265_RS06305 at 1549.920 kb on + strand, within CA265_RS06305 at 1549.993 kb on - strand at 1550.051 kb on - strand at 1550.086 kb on + strand at 1550.086 kb on + strand at 1550.148 kb on + strand at 1550.148 kb on + strand at 1550.149 kb on - strand at 1550.149 kb on - strand at 1550.208 kb on - strand, within CA265_RS06310 at 1550.309 kb on + strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.384 kb on - strand, within CA265_RS06310 at 1550.440 kb on + strand, within CA265_RS06310 at 1550.525 kb on + strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.603 kb on - strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.672 kb on - strand, within CA265_RS06310 at 1550.697 kb on - strand at 1550.714 kb on + strand at 1550.766 kb on + strand at 1550.794 kb on + strand at 1550.822 kb on + strand at 1550.834 kb on - strand at 1550.840 kb on - strand at 1550.886 kb on - strand at 1550.894 kb on + strand at 1550.895 kb on - strand at 1550.895 kb on - strand at 1551.014 kb on + strand, within CA265_RS06315 at 1551.015 kb on - strand, within CA265_RS06315 at 1551.018 kb on + strand, within CA265_RS06315 at 1551.018 kb on + strand, within CA265_RS06315 at 1551.058 kb on - strand, within CA265_RS06315 at 1551.063 kb on - strand, within CA265_RS06315 at 1551.104 kb on + strand, within CA265_RS06315 at 1551.116 kb on + strand, within CA265_RS06315 at 1551.151 kb on + strand, within CA265_RS06315 at 1551.159 kb on - strand, within CA265_RS06315 at 1551.211 kb on + strand, within CA265_RS06315 at 1551.212 kb on - strand, within CA265_RS06315 at 1551.268 kb on + strand, within CA265_RS06315 at 1551.405 kb on + strand, within CA265_RS06315 at 1551.408 kb on + strand, within CA265_RS06315 at 1551.468 kb on - strand at 1551.468 kb on - strand at 1551.512 kb on + strand at 1551.547 kb on + strand at 1551.547 kb on - strand at 1551.547 kb on - strand at 1551.554 kb on - strand at 1551.606 kb on + strand at 1551.678 kb on + strand at 1551.705 kb on + strand, within CA265_RS06320 at 1551.705 kb on + strand, within CA265_RS06320 at 1551.718 kb on + strand, within CA265_RS06320 at 1551.719 kb on - strand, within CA265_RS06320 at 1551.821 kb on - strand, within CA265_RS06320 at 1551.861 kb on + strand, within CA265_RS06320 at 1552.048 kb on + strand, within CA265_RS06320 at 1552.162 kb on - strand, within CA265_RS06320 at 1552.165 kb on - strand, within CA265_RS06320 at 1552.357 kb on + strand, within CA265_RS06320 at 1552.357 kb on + strand, within CA265_RS06320 at 1552.358 kb on - strand, within CA265_RS06320 at 1552.360 kb on + strand, within CA265_RS06320 at 1552.384 kb on - strand, within CA265_RS06320 at 1552.515 kb on + strand, within CA265_RS06320 at 1552.516 kb on - strand, within CA265_RS06320 at 1552.516 kb on - strand, within CA265_RS06320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 1,549,914 + CA265_RS06305 0.86 +0.2 1,549,915 - CA265_RS06305 0.86 +0.5 1,549,920 + CA265_RS06305 0.87 -0.2 1,549,993 - +0.1 1,550,051 - -0.2 1,550,086 + +1.2 1,550,086 + +1.2 1,550,148 + -0.4 1,550,148 + -0.2 1,550,149 - +0.5 1,550,149 - -0.6 1,550,208 - CA265_RS06310 0.18 +1.1 1,550,309 + CA265_RS06310 0.33 -0.2 1,550,331 - CA265_RS06310 0.36 +0.9 1,550,331 - CA265_RS06310 0.36 +0.2 1,550,384 - CA265_RS06310 0.44 +0.2 1,550,440 + CA265_RS06310 0.53 +0.6 1,550,525 + CA265_RS06310 0.66 -0.2 1,550,526 - CA265_RS06310 0.66 -0.0 1,550,526 - CA265_RS06310 0.66 +0.1 1,550,526 - CA265_RS06310 0.66 +0.0 1,550,603 - CA265_RS06310 0.78 +0.3 1,550,671 + CA265_RS06310 0.88 +0.0 1,550,671 + CA265_RS06310 0.88 +1.1 1,550,672 - CA265_RS06310 0.88 -0.2 1,550,697 - +0.1 1,550,714 + -0.7 1,550,766 + -0.5 1,550,794 + -0.1 1,550,822 + -0.2 1,550,834 - +0.3 1,550,840 - -0.4 1,550,886 - +0.0 1,550,894 + +0.9 1,550,895 - -0.5 1,550,895 - -0.1 1,551,014 + CA265_RS06315 0.20 -0.2 1,551,015 - CA265_RS06315 0.20 -0.1 1,551,018 + CA265_RS06315 0.20 -0.1 1,551,018 + CA265_RS06315 0.20 +0.1 1,551,058 - CA265_RS06315 0.26 -1.3 1,551,063 - CA265_RS06315 0.27 -0.5 1,551,104 + CA265_RS06315 0.33 +0.6 1,551,116 + CA265_RS06315 0.35 -0.8 1,551,151 + CA265_RS06315 0.41 +0.2 1,551,159 - CA265_RS06315 0.42 +0.5 1,551,211 + CA265_RS06315 0.50 +0.3 1,551,212 - CA265_RS06315 0.50 -0.2 1,551,268 + CA265_RS06315 0.59 -0.3 1,551,405 + CA265_RS06315 0.80 -0.5 1,551,408 + CA265_RS06315 0.81 -0.9 1,551,468 - +0.2 1,551,468 - +0.3 1,551,512 + -0.1 1,551,547 + +0.2 1,551,547 - +0.4 1,551,547 - -0.7 1,551,554 - -2.5 1,551,606 + -0.6 1,551,678 + -0.6 1,551,705 + CA265_RS06320 0.11 -0.1 1,551,705 + CA265_RS06320 0.11 -0.3 1,551,718 + CA265_RS06320 0.12 -1.3 1,551,719 - CA265_RS06320 0.12 +0.2 1,551,821 - CA265_RS06320 0.19 +1.2 1,551,861 + CA265_RS06320 0.21 -0.1 1,552,048 + CA265_RS06320 0.33 -0.8 1,552,162 - CA265_RS06320 0.41 -0.3 1,552,165 - CA265_RS06320 0.41 -0.9 1,552,357 + CA265_RS06320 0.53 -0.1 1,552,357 + CA265_RS06320 0.53 +0.2 1,552,358 - CA265_RS06320 0.53 +0.6 1,552,360 + CA265_RS06320 0.54 -0.6 1,552,384 - CA265_RS06320 0.55 +1.0 1,552,515 + CA265_RS06320 0.64 -0.6 1,552,516 - CA265_RS06320 0.64 -1.0 1,552,516 - CA265_RS06320 0.64 -0.5
Or see this region's nucleotide sequence