Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02420 and CA265_RS02425 are separated by 92 nucleotides CA265_RS02425 and CA265_RS02430 are separated by 6 nucleotides CA265_RS02430 and CA265_RS02435 are separated by 50 nucleotides CA265_RS02435 and CA265_RS02440 are separated by 132 nucleotides
CA265_RS02420: CA265_RS02420 - hypothetical protein, at 598,504 to 600,921
_RS02420
CA265_RS02425: CA265_RS02425 - GTP cyclohydrolase, at 601,014 to 601,304
_RS02425
CA265_RS02430: CA265_RS02430 - ABC transporter ATPase, at 601,311 to 601,790
_RS02430
CA265_RS02435: CA265_RS02435 - hypothetical protein, at 601,841 to 602,317
_RS02435
CA265_RS02440: CA265_RS02440 - TldD protein, at 602,450 to 604,087
_RS02440
Position (kb)
601
602 Strain fitness (log2 ratio)
-1
0
1 at 600.423 kb on + strand, within CA265_RS02420 at 600.424 kb on - strand, within CA265_RS02420 at 600.454 kb on + strand, within CA265_RS02420 at 600.510 kb on + strand, within CA265_RS02420 at 600.525 kb on - strand, within CA265_RS02420 at 600.532 kb on - strand, within CA265_RS02420 at 600.580 kb on - strand, within CA265_RS02420 at 600.588 kb on + strand, within CA265_RS02420 at 600.589 kb on - strand, within CA265_RS02420 at 600.596 kb on - strand, within CA265_RS02420 at 600.596 kb on - strand, within CA265_RS02420 at 600.601 kb on - strand, within CA265_RS02420 at 600.628 kb on + strand, within CA265_RS02420 at 600.629 kb on - strand, within CA265_RS02420 at 600.658 kb on - strand, within CA265_RS02420 at 600.676 kb on - strand, within CA265_RS02420 at 600.676 kb on - strand, within CA265_RS02420 at 600.697 kb on + strand at 600.830 kb on + strand at 600.831 kb on - strand at 600.883 kb on - strand at 600.916 kb on - strand at 600.940 kb on - strand at 600.940 kb on - strand at 600.983 kb on - strand at 600.987 kb on + strand at 600.992 kb on + strand at 600.992 kb on + strand at 600.992 kb on + strand at 600.993 kb on - strand at 600.993 kb on - strand at 600.993 kb on - strand at 601.059 kb on + strand, within CA265_RS02425 at 601.060 kb on - strand, within CA265_RS02425 at 601.211 kb on + strand, within CA265_RS02425 at 601.251 kb on - strand, within CA265_RS02425 at 601.283 kb on - strand at 601.287 kb on - strand at 601.308 kb on + strand at 601.308 kb on + strand at 601.317 kb on + strand at 601.401 kb on - strand, within CA265_RS02430 at 601.436 kb on + strand, within CA265_RS02430 at 601.436 kb on + strand, within CA265_RS02430 at 601.439 kb on + strand, within CA265_RS02430 at 601.440 kb on - strand, within CA265_RS02430 at 601.491 kb on + strand, within CA265_RS02430 at 601.498 kb on + strand, within CA265_RS02430 at 601.535 kb on - strand, within CA265_RS02430 at 601.710 kb on + strand, within CA265_RS02430 at 601.731 kb on - strand, within CA265_RS02430 at 601.737 kb on - strand, within CA265_RS02430 at 601.877 kb on - strand at 601.899 kb on + strand, within CA265_RS02435 at 601.899 kb on + strand, within CA265_RS02435 at 601.976 kb on - strand, within CA265_RS02435 at 602.007 kb on - strand, within CA265_RS02435 at 602.043 kb on - strand, within CA265_RS02435 at 602.057 kb on - strand, within CA265_RS02435 at 602.087 kb on - strand, within CA265_RS02435 at 602.087 kb on - strand, within CA265_RS02435 at 602.129 kb on - strand, within CA265_RS02435 at 602.129 kb on - strand, within CA265_RS02435 at 602.144 kb on + strand, within CA265_RS02435 at 602.262 kb on + strand, within CA265_RS02435 at 602.292 kb on + strand at 602.423 kb on - strand at 602.437 kb on - strand at 602.468 kb on - strand at 602.476 kb on - strand at 602.484 kb on + strand at 602.559 kb on - strand at 602.561 kb on - strand at 602.601 kb on + strand at 602.602 kb on - strand at 602.630 kb on + strand, within CA265_RS02440 at 602.686 kb on + strand, within CA265_RS02440 at 602.686 kb on + strand, within CA265_RS02440 at 602.686 kb on + strand, within CA265_RS02440 at 602.699 kb on - strand, within CA265_RS02440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 600,423 + CA265_RS02420 0.79 -1.1 600,424 - CA265_RS02420 0.79 +0.4 600,454 + CA265_RS02420 0.81 -0.2 600,510 + CA265_RS02420 0.83 -0.2 600,525 - CA265_RS02420 0.84 -0.6 600,532 - CA265_RS02420 0.84 +0.1 600,580 - CA265_RS02420 0.86 +0.4 600,588 + CA265_RS02420 0.86 +0.0 600,589 - CA265_RS02420 0.86 +0.3 600,596 - CA265_RS02420 0.87 -0.3 600,596 - CA265_RS02420 0.87 +0.7 600,601 - CA265_RS02420 0.87 +0.2 600,628 + CA265_RS02420 0.88 -0.3 600,629 - CA265_RS02420 0.88 +0.5 600,658 - CA265_RS02420 0.89 -0.6 600,676 - CA265_RS02420 0.90 +0.2 600,676 - CA265_RS02420 0.90 -0.3 600,697 + -0.7 600,830 + -1.3 600,831 - +0.0 600,883 - -0.3 600,916 - -1.5 600,940 - +0.5 600,940 - -0.9 600,983 - -0.1 600,987 + -0.3 600,992 + +0.9 600,992 + +0.7 600,992 + +0.7 600,993 - +0.2 600,993 - -0.4 600,993 - +0.3 601,059 + CA265_RS02425 0.15 +0.6 601,060 - CA265_RS02425 0.16 +0.5 601,211 + CA265_RS02425 0.68 -0.1 601,251 - CA265_RS02425 0.81 -0.2 601,283 - +0.8 601,287 - +0.3 601,308 + -1.2 601,308 + +0.6 601,317 + -0.9 601,401 - CA265_RS02430 0.19 +0.4 601,436 + CA265_RS02430 0.26 -0.5 601,436 + CA265_RS02430 0.26 +0.7 601,439 + CA265_RS02430 0.27 -0.1 601,440 - CA265_RS02430 0.27 -0.8 601,491 + CA265_RS02430 0.38 -1.0 601,498 + CA265_RS02430 0.39 -0.5 601,535 - CA265_RS02430 0.47 -0.6 601,710 + CA265_RS02430 0.83 -0.7 601,731 - CA265_RS02430 0.88 +0.0 601,737 - CA265_RS02430 0.89 -0.3 601,877 - -0.1 601,899 + CA265_RS02435 0.12 +0.6 601,899 + CA265_RS02435 0.12 -0.2 601,976 - CA265_RS02435 0.28 +0.4 602,007 - CA265_RS02435 0.35 -1.3 602,043 - CA265_RS02435 0.42 -0.1 602,057 - CA265_RS02435 0.45 -0.3 602,087 - CA265_RS02435 0.52 +0.7 602,087 - CA265_RS02435 0.52 -0.3 602,129 - CA265_RS02435 0.60 -1.1 602,129 - CA265_RS02435 0.60 +0.7 602,144 + CA265_RS02435 0.64 -0.4 602,262 + CA265_RS02435 0.88 -1.3 602,292 + +0.8 602,423 - -0.6 602,437 - -0.3 602,468 - -0.5 602,476 - -0.1 602,484 + +1.1 602,559 - -0.2 602,561 - +0.3 602,601 + -1.2 602,602 - -0.8 602,630 + CA265_RS02440 0.11 +0.2 602,686 + CA265_RS02440 0.14 -0.2 602,686 + CA265_RS02440 0.14 +0.9 602,686 + CA265_RS02440 0.14 -0.2 602,699 - CA265_RS02440 0.15 +0.1
Or see this region's nucleotide sequence