Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00600 and CA265_RS00605 are separated by 135 nucleotides CA265_RS00605 and CA265_RS00610 are separated by 355 nucleotides CA265_RS00610 and CA265_RS00615 are separated by 15 nucleotides
CA265_RS00600: CA265_RS00600 - recombinase RecA, at 136,471 to 137,484
_RS00600
CA265_RS00605: CA265_RS00605 - endonuclease III, at 137,620 to 138,285
_RS00605
CA265_RS00610: CA265_RS00610 - hypothetical protein, at 138,641 to 139,117
_RS00610
CA265_RS00615: CA265_RS00615 - copper oxidase, at 139,133 to 141,424
_RS00615
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1 at 136.632 kb on + strand, within CA265_RS00600 at 136.806 kb on + strand, within CA265_RS00600 at 136.810 kb on + strand, within CA265_RS00600 at 136.895 kb on - strand, within CA265_RS00600 at 136.911 kb on + strand, within CA265_RS00600 at 136.981 kb on + strand, within CA265_RS00600 at 137.065 kb on + strand, within CA265_RS00600 at 137.083 kb on + strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.119 kb on - strand, within CA265_RS00600 at 137.149 kb on - strand, within CA265_RS00600 at 137.202 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.244 kb on + strand, within CA265_RS00600 at 137.327 kb on - strand, within CA265_RS00600 at 137.334 kb on + strand, within CA265_RS00600 at 137.335 kb on - strand, within CA265_RS00600 at 137.407 kb on - strand at 137.442 kb on + strand at 137.530 kb on - strand at 137.530 kb on - strand at 137.669 kb on + strand at 137.669 kb on + strand at 137.681 kb on + strand at 137.682 kb on - strand at 137.682 kb on - strand at 137.729 kb on + strand, within CA265_RS00605 at 137.894 kb on + strand, within CA265_RS00605 at 137.900 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 137.975 kb on - strand, within CA265_RS00605 at 138.030 kb on + strand, within CA265_RS00605 at 138.133 kb on + strand, within CA265_RS00605 at 138.206 kb on + strand, within CA265_RS00605 at 138.211 kb on + strand, within CA265_RS00605 at 138.212 kb on - strand, within CA265_RS00605 at 138.296 kb on - strand at 138.316 kb on - strand at 138.413 kb on - strand at 138.427 kb on + strand at 138.474 kb on + strand at 138.486 kb on - strand at 138.486 kb on - strand at 138.490 kb on - strand at 138.511 kb on - strand at 138.619 kb on + strand at 138.749 kb on + strand, within CA265_RS00610 at 138.750 kb on - strand, within CA265_RS00610 at 138.757 kb on + strand, within CA265_RS00610 at 138.778 kb on + strand, within CA265_RS00610 at 138.806 kb on - strand, within CA265_RS00610 at 138.817 kb on + strand, within CA265_RS00610 at 138.874 kb on - strand, within CA265_RS00610 at 138.908 kb on + strand, within CA265_RS00610 at 138.975 kb on + strand, within CA265_RS00610 at 138.975 kb on + strand, within CA265_RS00610 at 138.976 kb on - strand, within CA265_RS00610 at 138.998 kb on + strand, within CA265_RS00610 at 139.007 kb on + strand, within CA265_RS00610 at 139.064 kb on + strand, within CA265_RS00610 at 139.075 kb on + strand at 139.093 kb on + strand at 139.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 136,632 + CA265_RS00600 0.16 +0.9 136,806 + CA265_RS00600 0.33 -2.6 136,810 + CA265_RS00600 0.33 +0.2 136,895 - CA265_RS00600 0.42 -0.6 136,911 + CA265_RS00600 0.43 -0.3 136,981 + CA265_RS00600 0.50 +0.2 137,065 + CA265_RS00600 0.59 -0.1 137,083 + CA265_RS00600 0.60 -0.8 137,119 - CA265_RS00600 0.64 -0.4 137,119 - CA265_RS00600 0.64 -0.7 137,149 - CA265_RS00600 0.67 -0.1 137,202 + CA265_RS00600 0.72 -0.9 137,244 + CA265_RS00600 0.76 +0.9 137,244 + CA265_RS00600 0.76 -1.0 137,244 + CA265_RS00600 0.76 -0.2 137,244 + CA265_RS00600 0.76 -0.5 137,327 - CA265_RS00600 0.84 -0.7 137,334 + CA265_RS00600 0.85 -2.7 137,335 - CA265_RS00600 0.85 -0.2 137,407 - +1.2 137,442 + -0.7 137,530 - -0.9 137,530 - -0.1 137,669 + +0.5 137,669 + -0.1 137,681 + -0.3 137,682 - -0.6 137,682 - -1.4 137,729 + CA265_RS00605 0.16 -1.4 137,894 + CA265_RS00605 0.41 -1.6 137,900 - CA265_RS00605 0.42 +0.0 137,975 - CA265_RS00605 0.53 -0.5 137,975 - CA265_RS00605 0.53 +0.6 138,030 + CA265_RS00605 0.62 -0.5 138,133 + CA265_RS00605 0.77 -0.0 138,206 + CA265_RS00605 0.88 -0.5 138,211 + CA265_RS00605 0.89 -2.2 138,212 - CA265_RS00605 0.89 +0.0 138,296 - +1.0 138,316 - -0.4 138,413 - +0.5 138,427 + +0.2 138,474 + -0.1 138,486 - +1.2 138,486 - +0.6 138,490 - -0.1 138,511 - +0.7 138,619 + -0.0 138,749 + CA265_RS00610 0.23 -0.3 138,750 - CA265_RS00610 0.23 +0.3 138,757 + CA265_RS00610 0.24 +0.9 138,778 + CA265_RS00610 0.29 +1.3 138,806 - CA265_RS00610 0.35 -1.8 138,817 + CA265_RS00610 0.37 +0.1 138,874 - CA265_RS00610 0.49 -1.3 138,908 + CA265_RS00610 0.56 -0.0 138,975 + CA265_RS00610 0.70 -1.0 138,975 + CA265_RS00610 0.70 -0.6 138,976 - CA265_RS00610 0.70 +0.0 138,998 + CA265_RS00610 0.75 -0.2 139,007 + CA265_RS00610 0.77 -0.5 139,064 + CA265_RS00610 0.89 +0.2 139,075 + -0.4 139,093 + -0.7 139,112 - +0.4
Or see this region's nucleotide sequence