Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3450 and Ac3H11_3451 are separated by 162 nucleotides Ac3H11_3451 and Ac3H11_3452 are separated by 161 nucleotides Ac3H11_3452 and Ac3H11_3648 are separated by 50 nucleotides Ac3H11_3648 and Ac3H11_3453 are separated by 30 nucleotides
Ac3H11_3450 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 243 to 851
_3450
Ac3H11_3451 - LSU ribosomal protein L25p, at 1,014 to 1,649
_3451
Ac3H11_3452 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 1,811 to 2,785
_3452
Ac3H11_3648 - tRNA-Gln-TTG, at 2,836 to 2,909
_3648
Ac3H11_3453 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 2,940 to 3,779
_3453
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 1.015 kb on + strand at 1.015 kb on + strand at 1.015 kb on + strand at 1.015 kb on + strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.331 kb on - strand, within Ac3H11_3451 at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,015 + +1.3 1,015 + -0.5 1,015 + -0.4 1,015 + -1.3 1,016 - -0.5 1,016 - -1.3 1,016 - +0.5 1,016 - -0.4 1,330 + Ac3H11_3451 0.50 -2.0 1,330 + Ac3H11_3451 0.50 -0.3 1,330 + Ac3H11_3451 0.50 -0.5 1,330 + Ac3H11_3451 0.50 +1.3 1,331 - Ac3H11_3451 0.50 -0.2 2,830 - +0.1 2,830 - +0.3 2,830 - +0.6 2,830 - -0.4 2,830 - -1.7
Or see this region's nucleotide sequence