Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14215 and EX31_RS14220 are separated by 330 nucleotides EX31_RS14220 and EX31_RS25365 are separated by 174 nucleotides EX31_RS25365 and EX31_RS14225 are separated by 201 nucleotides
EX31_RS14215: EX31_RS14215 - cystathionine gamma-synthase, at 23,291 to 24,451
_RS14215
EX31_RS14220: EX31_RS14220 - met regulon transcriptional regulator MetJ, at 24,782 to 25,099
_RS14220
EX31_RS25365: EX31_RS25365 - 50S ribosomal protein L31, at 25,274 to 25,486
_RS25365
EX31_RS14225: EX31_RS14225 - primosomal protein N', at 25,688 to 27,883
_RS14225
Position (kb) 25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 24.315 kb on + strand, within EX31_RS14215 at 24.315 kb on + strand, within EX31_RS14215 at 24.315 kb on + strand, within EX31_RS14215 at 24.315 kb on + strand, within EX31_RS14215 at 24.315 kb on + strand, within EX31_RS14215 at 24.315 kb on + strand, within EX31_RS14215 at 24.316 kb on - strand, within EX31_RS14215 at 24.316 kb on - strand, within EX31_RS14215 at 24.316 kb on - strand, within EX31_RS14215 at 24.352 kb on + strand at 24.352 kb on + strand at 24.352 kb on + strand at 24.353 kb on - strand at 24.353 kb on - strand at 24.353 kb on - strand at 24.353 kb on - strand at 24.353 kb on - strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.354 kb on + strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.355 kb on - strand at 24.358 kb on + strand at 24.359 kb on - strand at 24.359 kb on - strand at 24.359 kb on - strand at 24.359 kb on - strand at 24.369 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.383 kb on + strand at 24.384 kb on - strand at 24.384 kb on - strand at 24.384 kb on - strand at 24.384 kb on - strand at 24.384 kb on - strand at 24.393 kb on + strand at 24.394 kb on - strand at 24.394 kb on - strand at 24.451 kb on + strand at 24.451 kb on + strand at 24.452 kb on - strand at 24.488 kb on + strand at 24.488 kb on + strand at 24.488 kb on + strand at 24.489 kb on - strand at 24.489 kb on - strand at 24.490 kb on + strand at 24.490 kb on + strand at 24.490 kb on + strand at 24.490 kb on + strand at 24.490 kb on + strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.491 kb on - strand at 24.498 kb on - strand at 24.553 kb on - strand at 24.553 kb on - strand at 24.632 kb on + strand at 24.632 kb on + strand at 24.633 kb on - strand at 24.633 kb on - strand at 24.643 kb on + strand at 24.643 kb on + strand at 24.689 kb on + strand at 24.689 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.704 kb on - strand at 24.707 kb on + strand at 24.732 kb on + strand at 24.733 kb on - strand at 24.803 kb on + strand at 24.803 kb on + strand at 24.804 kb on - strand at 24.804 kb on - strand at 24.918 kb on + strand, within EX31_RS14220 at 24.919 kb on - strand, within EX31_RS14220 at 24.951 kb on + strand, within EX31_RS14220 at 24.951 kb on + strand, within EX31_RS14220 at 24.952 kb on - strand, within EX31_RS14220 at 25.015 kb on + strand, within EX31_RS14220 at 25.084 kb on + strand at 25.085 kb on - strand at 25.163 kb on + strand at 25.201 kb on + strand at 25.202 kb on - strand at 25.202 kb on - strand at 25.275 kb on + strand at 25.276 kb on - strand at 25.276 kb on - strand at 25.276 kb on - strand at 25.276 kb on - strand at 25.276 kb on - strand at 25.458 kb on - strand, within EX31_RS25365 at 25.458 kb on - strand, within EX31_RS25365 at 25.462 kb on - strand, within EX31_RS25365 at 25.596 kb on - strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.650 kb on + strand at 25.651 kb on - strand at 25.651 kb on - strand at 25.651 kb on - strand at 25.651 kb on - strand at 25.651 kb on - strand at 25.651 kb on - strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.655 kb on + strand at 25.656 kb on - strand at 25.656 kb on - strand at 25.656 kb on - strand at 25.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 24,315 + EX31_RS14215 0.88 -1.8 24,315 + EX31_RS14215 0.88 -1.4 24,315 + EX31_RS14215 0.88 -3.3 24,315 + EX31_RS14215 0.88 -2.1 24,315 + EX31_RS14215 0.88 -1.5 24,315 + EX31_RS14215 0.88 -2.0 24,316 - EX31_RS14215 0.88 -1.4 24,316 - EX31_RS14215 0.88 -0.5 24,316 - EX31_RS14215 0.88 -3.1 24,352 + -2.4 24,352 + -3.2 24,352 + -3.5 24,353 - -2.5 24,353 - -2.8 24,353 - -2.7 24,353 - -3.7 24,353 - -3.1 24,354 + -1.7 24,354 + -2.5 24,354 + -2.9 24,354 + -1.0 24,354 + -3.2 24,354 + -2.0 24,355 - -1.9 24,355 - -0.2 24,355 - -3.7 24,355 - -1.1 24,355 - -3.3 24,358 + -1.5 24,359 - -1.6 24,359 - +0.9 24,359 - -3.4 24,359 - -1.1 24,369 + -3.4 24,383 + -2.0 24,383 + -3.7 24,383 + -3.3 24,383 + -1.0 24,383 + -1.9 24,383 + -2.4 24,383 + -3.6 24,384 - +0.7 24,384 - -3.4 24,384 - -3.0 24,384 - -1.9 24,384 - -1.0 24,393 + -0.6 24,394 - -3.1 24,394 - -3.6 24,451 + -4.5 24,451 + -2.5 24,452 - -3.1 24,488 + -0.7 24,488 + +0.4 24,488 + -1.5 24,489 - +0.0 24,489 - +0.9 24,490 + +0.2 24,490 + +0.5 24,490 + -0.3 24,490 + +1.0 24,490 + +0.8 24,491 - -0.9 24,491 - -0.4 24,491 - -1.3 24,491 - -0.5 24,491 - -0.3 24,491 - -0.3 24,491 - +1.1 24,491 - +0.4 24,491 - -0.9 24,491 - -0.0 24,498 - -0.6 24,553 - +0.2 24,553 - +0.4 24,632 + +0.4 24,632 + -0.9 24,633 - +1.2 24,633 - -0.6 24,643 + +0.8 24,643 + +1.7 24,689 + -0.2 24,689 + +1.6 24,703 + -1.6 24,703 + -0.6 24,703 + -0.4 24,703 + +0.4 24,703 + -0.8 24,703 + -0.2 24,703 + +0.7 24,703 + +1.0 24,703 + -1.2 24,703 + -0.4 24,704 - +1.2 24,704 - -2.3 24,704 - +0.0 24,704 - -0.9 24,704 - +1.1 24,704 - -0.9 24,704 - -2.3 24,707 + +0.4 24,732 + -0.3 24,733 - +0.6 24,803 + +0.2 24,803 + -0.6 24,804 - +0.9 24,804 - -0.3 24,918 + EX31_RS14220 0.43 -2.6 24,919 - EX31_RS14220 0.43 +1.3 24,951 + EX31_RS14220 0.53 -2.6 24,951 + EX31_RS14220 0.53 -2.4 24,952 - EX31_RS14220 0.53 -0.8 25,015 + EX31_RS14220 0.73 +0.0 25,084 + +0.7 25,085 - -0.5 25,163 + +1.2 25,201 + -0.0 25,202 - +0.2 25,202 - +1.0 25,275 + +2.4 25,276 - -1.1 25,276 - +1.2 25,276 - +1.5 25,276 - -0.1 25,276 - +0.8 25,458 - EX31_RS25365 0.86 +0.1 25,458 - EX31_RS25365 0.86 -1.5 25,462 - EX31_RS25365 0.88 -1.0 25,596 - +0.4 25,650 + +0.5 25,650 + +0.1 25,650 + +0.4 25,650 + +0.5 25,650 + +0.0 25,650 + +0.1 25,650 + +0.2 25,650 + +1.4 25,650 + +1.2 25,650 + -0.5 25,651 - +0.9 25,651 - -0.8 25,651 - -1.1 25,651 - -0.9 25,651 - +0.3 25,651 - +1.6 25,655 + +0.5 25,655 + +0.9 25,655 + +0.5 25,655 + +0.3 25,655 + +0.2 25,655 + +0.1 25,655 + +0.1 25,655 + +0.1 25,655 + -1.4 25,655 + +1.5 25,655 + +0.2 25,655 + -0.1 25,655 + -0.4 25,656 - +1.1 25,656 - +0.1 25,656 - -0.6 25,673 + +0.1
Or see this region's nucleotide sequence