Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23045 and EX31_RS23050 are separated by 212 nucleotides EX31_RS23050 and EX31_RS23055 are separated by 4 nucleotides EX31_RS23055 and EX31_RS23060 are separated by 210 nucleotides
EX31_RS23045: EX31_RS23045 - fluoride efflux transporter CrcB, at 371,661 to 372,044
_RS23045
EX31_RS23050: EX31_RS23050 - GNAT family N-acetyltransferase, at 372,257 to 372,679
_RS23050
EX31_RS23055: EX31_RS23055 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 372,684 to 374,030
_RS23055
EX31_RS23060: EX31_RS23060 - nitrate ABC transporter substrate-binding protein, at 374,241 to 375,197
_RS23060
Position (kb) 372
373
374
375 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 371.690 kb on + strand at 371.690 kb on + strand at 371.691 kb on - strand at 371.691 kb on - strand at 371.700 kb on + strand, within EX31_RS23045 at 371.742 kb on + strand, within EX31_RS23045 at 371.742 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.772 kb on + strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.773 kb on - strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.779 kb on + strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.780 kb on - strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.983 kb on + strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 371.984 kb on - strand, within EX31_RS23045 at 372.005 kb on + strand, within EX31_RS23045 at 372.030 kb on + strand at 372.031 kb on - strand at 372.031 kb on - strand at 372.031 kb on - strand at 372.038 kb on + strand at 372.038 kb on + strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.039 kb on - strand at 372.044 kb on + strand at 372.045 kb on - strand at 372.045 kb on - strand at 372.045 kb on - strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.050 kb on + strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.051 kb on - strand at 372.063 kb on - strand at 372.095 kb on + strand at 372.135 kb on + strand at 372.176 kb on + strand at 372.177 kb on - strand at 372.177 kb on - strand at 372.177 kb on - strand at 372.177 kb on - strand at 372.217 kb on + strand at 372.218 kb on - strand at 372.218 kb on - strand at 372.261 kb on + strand at 372.261 kb on + strand at 372.393 kb on + strand, within EX31_RS23050 at 372.485 kb on + strand, within EX31_RS23050 at 372.485 kb on + strand, within EX31_RS23050 at 372.485 kb on + strand, within EX31_RS23050 at 372.485 kb on + strand, within EX31_RS23050 at 372.485 kb on + strand, within EX31_RS23050 at 372.660 kb on - strand at 372.660 kb on - strand at 372.660 kb on - strand at 372.660 kb on - strand at 372.758 kb on + strand at 372.947 kb on - strand, within EX31_RS23055 at 372.947 kb on - strand, within EX31_RS23055 at 372.966 kb on - strand, within EX31_RS23055 at 373.120 kb on + strand, within EX31_RS23055 at 373.132 kb on + strand, within EX31_RS23055 at 373.235 kb on + strand, within EX31_RS23055 at 373.235 kb on + strand, within EX31_RS23055 at 373.236 kb on - strand, within EX31_RS23055 at 373.236 kb on - strand, within EX31_RS23055 at 373.267 kb on + strand, within EX31_RS23055 at 373.268 kb on - strand, within EX31_RS23055 at 373.268 kb on - strand, within EX31_RS23055 at 373.307 kb on + strand, within EX31_RS23055 at 373.308 kb on - strand, within EX31_RS23055 at 373.609 kb on + strand, within EX31_RS23055 at 373.610 kb on - strand, within EX31_RS23055 at 373.709 kb on + strand, within EX31_RS23055 at 373.709 kb on + strand, within EX31_RS23055 at 373.709 kb on + strand, within EX31_RS23055 at 373.709 kb on + strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.710 kb on - strand, within EX31_RS23055 at 373.902 kb on - strand at 373.974 kb on + strand at 374.067 kb on + strand at 374.087 kb on + strand at 374.088 kb on - strand at 374.091 kb on - strand at 374.100 kb on - strand at 374.120 kb on + strand at 374.149 kb on + strand at 374.149 kb on + strand at 374.149 kb on + strand at 374.149 kb on + strand at 374.149 kb on + strand at 374.149 kb on + strand at 374.150 kb on - strand at 374.150 kb on - strand at 374.153 kb on - strand at 374.153 kb on - strand at 374.209 kb on + strand at 374.209 kb on + strand at 374.210 kb on - strand at 374.213 kb on - strand at 374.213 kb on - strand at 374.213 kb on - strand at 374.228 kb on + strand at 374.228 kb on + strand at 374.228 kb on + strand at 374.228 kb on + strand at 374.228 kb on + strand at 374.228 kb on + strand at 374.229 kb on - strand at 374.229 kb on - strand at 374.229 kb on - strand at 374.229 kb on - strand at 374.249 kb on + strand at 374.250 kb on - strand at 374.250 kb on - strand at 374.281 kb on - strand at 374.300 kb on - strand at 374.335 kb on + strand at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.382 kb on + strand, within EX31_RS23060 at 374.383 kb on - strand, within EX31_RS23060 at 374.383 kb on - strand, within EX31_RS23060 at 374.530 kb on - strand, within EX31_RS23060 at 374.640 kb on + strand, within EX31_RS23060 at 374.640 kb on + strand, within EX31_RS23060 at 374.640 kb on + strand, within EX31_RS23060 at 374.641 kb on - strand, within EX31_RS23060 at 374.735 kb on + strand, within EX31_RS23060 at 374.735 kb on + strand, within EX31_RS23060 at 374.736 kb on - strand, within EX31_RS23060 at 374.749 kb on + strand, within EX31_RS23060 at 374.750 kb on - strand, within EX31_RS23060 at 374.773 kb on + strand, within EX31_RS23060 at 374.777 kb on + strand, within EX31_RS23060 at 374.777 kb on + strand, within EX31_RS23060 at 374.777 kb on + strand, within EX31_RS23060 at 374.778 kb on - strand, within EX31_RS23060 at 374.786 kb on + strand, within EX31_RS23060 at 374.787 kb on - strand, within EX31_RS23060 at 374.862 kb on + strand, within EX31_RS23060 at 374.880 kb on + strand, within EX31_RS23060 at 374.880 kb on + strand, within EX31_RS23060 at 374.976 kb on + strand, within EX31_RS23060 at 374.976 kb on + strand, within EX31_RS23060 at 374.976 kb on + strand, within EX31_RS23060 at 374.993 kb on + strand, within EX31_RS23060 at 375.006 kb on + strand, within EX31_RS23060
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 371,690 + +0.8 371,690 + +0.8 371,691 - -1.4 371,691 - -0.7 371,700 + EX31_RS23045 0.10 -0.3 371,742 + EX31_RS23045 0.21 +1.0 371,742 + EX31_RS23045 0.21 +3.3 371,772 + EX31_RS23045 0.29 +1.9 371,772 + EX31_RS23045 0.29 +0.2 371,772 + EX31_RS23045 0.29 +0.2 371,772 + EX31_RS23045 0.29 -1.2 371,772 + EX31_RS23045 0.29 +0.6 371,773 - EX31_RS23045 0.29 +1.3 371,773 - EX31_RS23045 0.29 -1.2 371,773 - EX31_RS23045 0.29 +0.9 371,779 + EX31_RS23045 0.31 -0.0 371,779 + EX31_RS23045 0.31 -1.0 371,779 + EX31_RS23045 0.31 -0.7 371,779 + EX31_RS23045 0.31 -0.7 371,779 + EX31_RS23045 0.31 -0.1 371,779 + EX31_RS23045 0.31 -0.7 371,779 + EX31_RS23045 0.31 +1.1 371,779 + EX31_RS23045 0.31 -1.6 371,780 - EX31_RS23045 0.31 -1.2 371,780 - EX31_RS23045 0.31 -0.3 371,780 - EX31_RS23045 0.31 -1.5 371,780 - EX31_RS23045 0.31 -1.3 371,780 - EX31_RS23045 0.31 -0.6 371,780 - EX31_RS23045 0.31 -0.2 371,780 - EX31_RS23045 0.31 -0.1 371,983 + EX31_RS23045 0.84 +0.2 371,983 + EX31_RS23045 0.84 -0.6 371,983 + EX31_RS23045 0.84 +0.7 371,984 - EX31_RS23045 0.84 +1.0 371,984 - EX31_RS23045 0.84 +2.3 371,984 - EX31_RS23045 0.84 -0.5 371,984 - EX31_RS23045 0.84 +0.1 371,984 - EX31_RS23045 0.84 -0.1 372,005 + EX31_RS23045 0.90 -1.3 372,030 + -0.2 372,031 - +0.3 372,031 - +0.6 372,031 - -0.0 372,038 + +1.1 372,038 + -2.3 372,039 - -0.5 372,039 - -1.9 372,039 - +0.3 372,039 - -0.4 372,039 - +0.4 372,044 + +1.5 372,045 - -0.1 372,045 - +1.1 372,045 - -1.6 372,050 + +0.1 372,050 + +0.2 372,050 + -0.4 372,050 + +0.1 372,050 + +0.5 372,051 - +1.6 372,051 - -0.4 372,051 - +0.0 372,051 - -0.0 372,051 - -0.9 372,051 - +0.7 372,051 - +0.7 372,063 - +1.9 372,095 + -0.7 372,135 + +0.2 372,176 + -0.2 372,177 - +0.0 372,177 - +0.3 372,177 - -0.4 372,177 - +1.9 372,217 + -0.7 372,218 - +1.9 372,218 - -1.4 372,261 + +0.9 372,261 + +0.3 372,393 + EX31_RS23050 0.32 -0.6 372,485 + EX31_RS23050 0.54 -0.1 372,485 + EX31_RS23050 0.54 -0.9 372,485 + EX31_RS23050 0.54 -0.1 372,485 + EX31_RS23050 0.54 +0.2 372,485 + EX31_RS23050 0.54 +0.2 372,660 - -1.3 372,660 - -1.0 372,660 - +0.7 372,660 - -2.9 372,758 + -0.0 372,947 - EX31_RS23055 0.20 -0.2 372,947 - EX31_RS23055 0.20 -1.1 372,966 - EX31_RS23055 0.21 -0.1 373,120 + EX31_RS23055 0.32 +0.5 373,132 + EX31_RS23055 0.33 +1.7 373,235 + EX31_RS23055 0.41 +0.7 373,235 + EX31_RS23055 0.41 -0.6 373,236 - EX31_RS23055 0.41 +0.3 373,236 - EX31_RS23055 0.41 +0.3 373,267 + EX31_RS23055 0.43 +1.9 373,268 - EX31_RS23055 0.43 -0.1 373,268 - EX31_RS23055 0.43 -0.3 373,307 + EX31_RS23055 0.46 -0.7 373,308 - EX31_RS23055 0.46 +0.0 373,609 + EX31_RS23055 0.69 +1.7 373,610 - EX31_RS23055 0.69 +1.0 373,709 + EX31_RS23055 0.76 +0.2 373,709 + EX31_RS23055 0.76 -0.3 373,709 + EX31_RS23055 0.76 +0.4 373,709 + EX31_RS23055 0.76 +0.7 373,710 - EX31_RS23055 0.76 +1.4 373,710 - EX31_RS23055 0.76 +1.2 373,710 - EX31_RS23055 0.76 -0.1 373,710 - EX31_RS23055 0.76 +1.6 373,710 - EX31_RS23055 0.76 +0.0 373,710 - EX31_RS23055 0.76 +0.4 373,710 - EX31_RS23055 0.76 +0.8 373,710 - EX31_RS23055 0.76 +0.1 373,710 - EX31_RS23055 0.76 -0.6 373,902 - +0.9 373,974 + +0.3 374,067 + +1.0 374,087 + -0.6 374,088 - -0.2 374,091 - +0.1 374,100 - +0.8 374,120 + +1.0 374,149 + -0.5 374,149 + +1.5 374,149 + +1.1 374,149 + +0.3 374,149 + -0.2 374,149 + +0.4 374,150 - +0.7 374,150 - -0.8 374,153 - -1.9 374,153 - +0.3 374,209 + -0.7 374,209 + +1.2 374,210 - +0.5 374,213 - +1.3 374,213 - +2.3 374,213 - +2.2 374,228 + -0.5 374,228 + +0.0 374,228 + +1.9 374,228 + +0.6 374,228 + +0.0 374,228 + -1.6 374,229 - +1.7 374,229 - +0.9 374,229 - +0.6 374,229 - +0.2 374,249 + +0.5 374,250 - +1.1 374,250 - -0.4 374,281 - -0.2 374,300 - -0.9 374,335 + +0.6 374,382 + EX31_RS23060 0.15 +0.4 374,382 + EX31_RS23060 0.15 +0.5 374,382 + EX31_RS23060 0.15 +0.7 374,382 + EX31_RS23060 0.15 +0.7 374,382 + EX31_RS23060 0.15 +0.8 374,382 + EX31_RS23060 0.15 +0.8 374,382 + EX31_RS23060 0.15 +0.8 374,382 + EX31_RS23060 0.15 +1.1 374,382 + EX31_RS23060 0.15 -1.4 374,382 + EX31_RS23060 0.15 -1.3 374,383 - EX31_RS23060 0.15 -2.2 374,383 - EX31_RS23060 0.15 +0.3 374,530 - EX31_RS23060 0.30 +0.3 374,640 + EX31_RS23060 0.42 +0.1 374,640 + EX31_RS23060 0.42 +0.7 374,640 + EX31_RS23060 0.42 -0.3 374,641 - EX31_RS23060 0.42 +1.1 374,735 + EX31_RS23060 0.52 -0.9 374,735 + EX31_RS23060 0.52 +0.9 374,736 - EX31_RS23060 0.52 +0.8 374,749 + EX31_RS23060 0.53 +0.4 374,750 - EX31_RS23060 0.53 +0.2 374,773 + EX31_RS23060 0.56 +1.4 374,777 + EX31_RS23060 0.56 -1.1 374,777 + EX31_RS23060 0.56 -0.4 374,777 + EX31_RS23060 0.56 +0.1 374,778 - EX31_RS23060 0.56 +1.6 374,786 + EX31_RS23060 0.57 -1.0 374,787 - EX31_RS23060 0.57 -0.4 374,862 + EX31_RS23060 0.65 +0.3 374,880 + EX31_RS23060 0.67 +0.7 374,880 + EX31_RS23060 0.67 +0.2 374,976 + EX31_RS23060 0.77 -1.2 374,976 + EX31_RS23060 0.77 -0.2 374,976 + EX31_RS23060 0.77 -0.3 374,993 + EX31_RS23060 0.79 +0.2 375,006 + EX31_RS23060 0.80 +1.4
Or see this region's nucleotide sequence