Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22325 and EX31_RS22330 are separated by 271 nucleotides EX31_RS22330 and EX31_RS22335 are separated by 67 nucleotides EX31_RS22335 and EX31_RS22340 are separated by 396 nucleotides
EX31_RS22325: EX31_RS22325 - acyl-CoA thioesterase II, at 218,551 to 219,417
_RS22325
EX31_RS22330: EX31_RS22330 - hypothetical protein, at 219,689 to 220,270
_RS22330
EX31_RS22335: EX31_RS22335 - MGMT family protein, at 220,338 to 220,646
_RS22335
EX31_RS22340: EX31_RS22340 - site-specific integrase, at 221,043 to 222,134
_RS22340
Position (kb) 219
220
221 Strain fitness (log2 ratio) -2
-1
0
1
2 at 218.817 kb on - strand, within EX31_RS22325 at 218.817 kb on - strand, within EX31_RS22325 at 218.825 kb on - strand, within EX31_RS22325 at 218.829 kb on - strand, within EX31_RS22325 at 218.947 kb on - strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.952 kb on + strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 218.953 kb on - strand, within EX31_RS22325 at 219.026 kb on - strand, within EX31_RS22325 at 219.026 kb on - strand, within EX31_RS22325 at 219.108 kb on + strand, within EX31_RS22325 at 219.109 kb on - strand, within EX31_RS22325 at 219.111 kb on - strand, within EX31_RS22325 at 219.166 kb on - strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.191 kb on + strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.192 kb on - strand, within EX31_RS22325 at 219.195 kb on + strand, within EX31_RS22325 at 219.196 kb on - strand, within EX31_RS22325 at 219.196 kb on - strand, within EX31_RS22325 at 219.250 kb on - strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.284 kb on + strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.285 kb on - strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.286 kb on + strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.287 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.326 kb on - strand, within EX31_RS22325 at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.452 kb on + strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.453 kb on - strand at 219.466 kb on + strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.492 kb on - strand at 219.511 kb on + strand at 219.512 kb on - strand at 219.512 kb on - strand at 219.519 kb on + strand at 219.520 kb on - strand at 219.525 kb on + strand at 219.535 kb on + strand at 219.535 kb on + strand at 219.535 kb on + strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.536 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.599 kb on - strand at 219.599 kb on - strand at 219.599 kb on - strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.601 kb on + strand at 219.602 kb on - strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.605 kb on + strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.606 kb on - strand at 219.624 kb on - strand at 219.646 kb on - strand at 219.646 kb on - strand at 219.666 kb on + strand at 219.681 kb on + strand at 219.682 kb on - strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.705 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.708 kb on + strand at 219.709 kb on - strand at 219.709 kb on - strand at 219.842 kb on - strand, within EX31_RS22330 at 219.999 kb on - strand, within EX31_RS22330 at 220.000 kb on + strand, within EX31_RS22330 at 220.000 kb on + strand, within EX31_RS22330 at 220.001 kb on - strand, within EX31_RS22330 at 220.021 kb on + strand, within EX31_RS22330 at 220.021 kb on + strand, within EX31_RS22330 at 220.031 kb on - strand, within EX31_RS22330 at 220.118 kb on - strand, within EX31_RS22330 at 220.241 kb on - strand at 220.266 kb on - strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.268 kb on + strand at 220.269 kb on - strand at 220.269 kb on - strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.280 kb on + strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.281 kb on - strand at 220.289 kb on + strand at 220.290 kb on - strand at 220.354 kb on + strand at 220.355 kb on - strand at 220.383 kb on - strand, within EX31_RS22335 at 220.424 kb on + strand, within EX31_RS22335 at 220.513 kb on - strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.570 kb on + strand, within EX31_RS22335 at 220.571 kb on - strand, within EX31_RS22335 at 220.571 kb on - strand, within EX31_RS22335 at 220.614 kb on + strand, within EX31_RS22335 at 220.615 kb on - strand, within EX31_RS22335 at 220.674 kb on + strand at 220.674 kb on + strand at 220.675 kb on - strand at 220.699 kb on - strand at 220.716 kb on + strand at 220.716 kb on + strand at 220.717 kb on - strand at 220.748 kb on + strand at 220.748 kb on + strand at 220.749 kb on - strand at 220.801 kb on + strand at 220.974 kb on + strand at 221.009 kb on + strand at 221.010 kb on - strand at 221.022 kb on + strand at 221.022 kb on + strand at 221.029 kb on + strand at 221.030 kb on - strand at 221.030 kb on - strand at 221.054 kb on + strand at 221.054 kb on + strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.055 kb on - strand at 221.079 kb on + strand at 221.079 kb on + strand at 221.095 kb on - strand at 221.179 kb on - strand, within EX31_RS22340
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 218,817 - EX31_RS22325 0.31 +1.0 218,817 - EX31_RS22325 0.31 +0.3 218,825 - EX31_RS22325 0.32 -1.6 218,829 - EX31_RS22325 0.32 +0.9 218,947 - EX31_RS22325 0.46 -1.6 218,952 + EX31_RS22325 0.46 +0.6 218,952 + EX31_RS22325 0.46 -1.4 218,952 + EX31_RS22325 0.46 +0.3 218,952 + EX31_RS22325 0.46 -0.7 218,953 - EX31_RS22325 0.46 +0.3 218,953 - EX31_RS22325 0.46 -0.4 218,953 - EX31_RS22325 0.46 +0.3 218,953 - EX31_RS22325 0.46 +1.5 219,026 - EX31_RS22325 0.55 +0.6 219,026 - EX31_RS22325 0.55 +0.0 219,108 + EX31_RS22325 0.64 +1.0 219,109 - EX31_RS22325 0.64 -0.6 219,111 - EX31_RS22325 0.65 -0.0 219,166 - EX31_RS22325 0.71 -0.5 219,191 + EX31_RS22325 0.74 +0.1 219,191 + EX31_RS22325 0.74 +1.1 219,191 + EX31_RS22325 0.74 -0.1 219,192 - EX31_RS22325 0.74 +0.0 219,192 - EX31_RS22325 0.74 -0.4 219,192 - EX31_RS22325 0.74 -0.2 219,192 - EX31_RS22325 0.74 +0.4 219,192 - EX31_RS22325 0.74 -0.4 219,192 - EX31_RS22325 0.74 +0.3 219,195 + EX31_RS22325 0.74 +1.6 219,196 - EX31_RS22325 0.74 -0.4 219,196 - EX31_RS22325 0.74 -1.6 219,250 - EX31_RS22325 0.81 -0.3 219,284 + EX31_RS22325 0.85 -0.3 219,284 + EX31_RS22325 0.85 +1.0 219,284 + EX31_RS22325 0.85 +1.0 219,284 + EX31_RS22325 0.85 -0.6 219,284 + EX31_RS22325 0.85 +1.4 219,284 + EX31_RS22325 0.85 +1.7 219,285 - EX31_RS22325 0.85 +0.6 219,285 - EX31_RS22325 0.85 -0.3 219,285 - EX31_RS22325 0.85 +0.6 219,285 - EX31_RS22325 0.85 +0.5 219,285 - EX31_RS22325 0.85 +0.2 219,285 - EX31_RS22325 0.85 +0.3 219,286 + EX31_RS22325 0.85 +0.7 219,286 + EX31_RS22325 0.85 +1.0 219,286 + EX31_RS22325 0.85 -1.2 219,286 + EX31_RS22325 0.85 -0.0 219,287 - EX31_RS22325 0.85 -0.2 219,287 - EX31_RS22325 0.85 -0.8 219,287 - EX31_RS22325 0.85 -1.7 219,287 - EX31_RS22325 0.85 -1.3 219,287 - EX31_RS22325 0.85 -0.6 219,287 - EX31_RS22325 0.85 +1.5 219,287 - EX31_RS22325 0.85 -0.4 219,287 - EX31_RS22325 0.85 -0.6 219,287 - EX31_RS22325 0.85 -0.2 219,287 - EX31_RS22325 0.85 +0.7 219,287 - EX31_RS22325 0.85 -0.9 219,287 - EX31_RS22325 0.85 +0.4 219,287 - EX31_RS22325 0.85 +0.1 219,326 - EX31_RS22325 0.89 -0.9 219,326 - EX31_RS22325 0.89 +0.0 219,326 - EX31_RS22325 0.89 -0.0 219,452 + +0.6 219,452 + -0.5 219,452 + -0.2 219,452 + +0.7 219,452 + +0.6 219,452 + +0.8 219,452 + +0.3 219,452 + -0.2 219,452 + +2.0 219,453 - -0.0 219,453 - +0.8 219,453 - -0.7 219,453 - -1.7 219,453 - -0.0 219,453 - +0.4 219,453 - +0.5 219,466 + +0.9 219,492 - -0.0 219,492 - -2.2 219,492 - +1.0 219,492 - -0.8 219,511 + -0.2 219,512 - +0.5 219,512 - +1.0 219,519 + -0.6 219,520 - -0.2 219,525 + +0.2 219,535 + +0.2 219,535 + -0.1 219,535 + +0.2 219,536 - +0.7 219,536 - -0.4 219,536 - +0.4 219,536 - -0.3 219,536 - -0.8 219,598 + +2.4 219,598 + -0.1 219,599 - -0.7 219,599 - -0.1 219,599 - -0.0 219,601 + -0.2 219,601 + -0.3 219,601 + +0.1 219,601 + +0.1 219,602 - +0.2 219,605 + +0.3 219,605 + -0.2 219,605 + +1.5 219,605 + -1.5 219,605 + +0.2 219,605 + -0.1 219,605 + +1.2 219,605 + -1.2 219,605 + +0.4 219,606 - -0.8 219,606 - -1.3 219,606 - -0.5 219,606 - -1.2 219,606 - -1.3 219,606 - +0.2 219,606 - +2.0 219,624 - -0.5 219,646 - -0.0 219,646 - -0.1 219,666 + +1.0 219,681 + +0.2 219,682 - +0.2 219,705 + -0.5 219,705 + -1.6 219,705 + -0.9 219,705 + +1.6 219,708 + +1.1 219,708 + +2.0 219,708 + +0.7 219,708 + -0.2 219,709 - +1.4 219,709 - +0.5 219,842 - EX31_RS22330 0.26 -1.2 219,999 - EX31_RS22330 0.53 +0.7 220,000 + EX31_RS22330 0.53 +0.8 220,000 + EX31_RS22330 0.53 -0.4 220,001 - EX31_RS22330 0.54 -0.1 220,021 + EX31_RS22330 0.57 +0.0 220,021 + EX31_RS22330 0.57 -0.3 220,031 - EX31_RS22330 0.59 +0.2 220,118 - EX31_RS22330 0.74 +0.7 220,241 - +0.2 220,266 - +0.2 220,268 + -0.0 220,268 + +0.6 220,268 + +0.8 220,269 - +0.7 220,269 - +0.6 220,280 + +0.1 220,280 + -1.2 220,280 + -1.4 220,280 + +0.8 220,280 + -2.4 220,280 + -0.4 220,280 + -1.1 220,280 + +0.6 220,280 + -0.2 220,280 + -0.0 220,280 + +0.8 220,280 + +0.1 220,280 + +0.3 220,280 + +1.7 220,281 - +0.2 220,281 - -0.2 220,281 - +0.5 220,281 - -0.1 220,281 - +1.0 220,281 - +0.0 220,281 - +0.5 220,281 - +2.6 220,281 - -0.0 220,281 - +0.4 220,281 - +0.2 220,281 - -0.0 220,289 + -0.9 220,290 - -0.6 220,354 + +1.1 220,355 - -0.2 220,383 - EX31_RS22335 0.15 -0.0 220,424 + EX31_RS22335 0.28 -2.1 220,513 - EX31_RS22335 0.57 +1.6 220,570 + EX31_RS22335 0.75 -0.7 220,570 + EX31_RS22335 0.75 -1.6 220,570 + EX31_RS22335 0.75 -1.2 220,570 + EX31_RS22335 0.75 -0.4 220,571 - EX31_RS22335 0.75 +0.4 220,571 - EX31_RS22335 0.75 -0.4 220,614 + EX31_RS22335 0.89 -0.9 220,615 - EX31_RS22335 0.90 +0.5 220,674 + +0.7 220,674 + +0.6 220,675 - +1.0 220,699 - -0.0 220,716 + -0.3 220,716 + +0.4 220,717 - -0.8 220,748 + +0.4 220,748 + -0.6 220,749 - +0.2 220,801 + +1.5 220,974 + +0.4 221,009 + -0.1 221,010 - -0.8 221,022 + -0.4 221,022 + +0.3 221,029 + +0.7 221,030 - -0.4 221,030 - +0.5 221,054 + -1.0 221,054 + +0.4 221,055 - +1.1 221,055 - +0.4 221,055 - +0.1 221,055 - +0.1 221,055 - -0.0 221,055 - +0.4 221,079 + +1.6 221,079 + +0.5 221,095 - +0.2 221,179 - EX31_RS22340 0.12 -0.4
Or see this region's nucleotide sequence