Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13500 and EX31_RS13505 are separated by 48 nucleotides EX31_RS13505 and EX31_RS13510 are separated by 184 nucleotides
EX31_RS13500: EX31_RS13500 - tRNA-modifying protein YgfZ, at 133,647 to 134,639
_RS13500
EX31_RS13505: EX31_RS13505 - class IV adenylate cyclase, at 134,688 to 135,218
_RS13505
EX31_RS13510: EX31_RS13510 - 6-phosphofructokinase II, at 135,403 to 136,347
_RS13510
Position (kb) 134
135
136 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 134.637 kb on + strand at 134.638 kb on - strand at 134.638 kb on - strand at 134.638 kb on - strand at 134.638 kb on - strand at 134.649 kb on + strand at 134.649 kb on + strand at 134.649 kb on + strand at 134.649 kb on + strand at 134.649 kb on + strand at 134.649 kb on + strand at 134.650 kb on - strand at 134.650 kb on - strand at 134.650 kb on - strand at 134.682 kb on - strand at 134.683 kb on + strand at 134.683 kb on + strand at 134.683 kb on + strand at 134.684 kb on - strand at 134.731 kb on + strand at 134.731 kb on + strand at 134.732 kb on - strand at 134.732 kb on - strand at 134.732 kb on - strand at 134.732 kb on - strand at 134.778 kb on + strand, within EX31_RS13505 at 134.778 kb on + strand, within EX31_RS13505 at 134.779 kb on - strand, within EX31_RS13505 at 134.865 kb on + strand, within EX31_RS13505 at 134.866 kb on - strand, within EX31_RS13505 at 134.866 kb on - strand, within EX31_RS13505 at 134.942 kb on - strand, within EX31_RS13505 at 134.998 kb on + strand, within EX31_RS13505 at 134.998 kb on + strand, within EX31_RS13505 at 134.999 kb on - strand, within EX31_RS13505 at 134.999 kb on - strand, within EX31_RS13505 at 134.999 kb on - strand, within EX31_RS13505 at 135.166 kb on + strand at 135.167 kb on - strand at 135.186 kb on + strand at 135.186 kb on + strand at 135.187 kb on - strand at 135.230 kb on + strand at 135.230 kb on + strand at 135.230 kb on + strand at 135.249 kb on - strand at 135.251 kb on - strand at 135.251 kb on - strand at 135.251 kb on - strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.278 kb on + strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.279 kb on - strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.289 kb on + strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.290 kb on - strand at 135.324 kb on + strand at 135.325 kb on - strand at 135.364 kb on + strand at 135.364 kb on + strand at 135.364 kb on + strand at 135.402 kb on - strand at 135.413 kb on + strand at 135.413 kb on + strand at 135.414 kb on - strand at 135.414 kb on - strand at 135.414 kb on - strand at 135.414 kb on - strand at 135.463 kb on - strand at 135.469 kb on + strand at 135.480 kb on + strand at 135.481 kb on - strand at 135.492 kb on + strand at 135.492 kb on + strand at 135.492 kb on + strand at 135.493 kb on - strand at 135.493 kb on - strand at 135.528 kb on + strand, within EX31_RS13510 at 135.528 kb on + strand, within EX31_RS13510 at 135.528 kb on + strand, within EX31_RS13510 at 135.529 kb on - strand, within EX31_RS13510 at 135.529 kb on - strand, within EX31_RS13510 at 135.529 kb on - strand, within EX31_RS13510 at 135.529 kb on - strand, within EX31_RS13510 at 135.529 kb on - strand, within EX31_RS13510 at 135.533 kb on + strand, within EX31_RS13510 at 135.533 kb on + strand, within EX31_RS13510 at 135.550 kb on + strand, within EX31_RS13510 at 135.575 kb on + strand, within EX31_RS13510 at 135.699 kb on + strand, within EX31_RS13510 at 135.742 kb on - strand, within EX31_RS13510 at 135.742 kb on - strand, within EX31_RS13510 at 135.742 kb on - strand, within EX31_RS13510 at 135.742 kb on - strand, within EX31_RS13510 at 135.765 kb on + strand, within EX31_RS13510 at 135.840 kb on + strand, within EX31_RS13510 at 135.841 kb on - strand, within EX31_RS13510 at 135.857 kb on + strand, within EX31_RS13510 at 135.857 kb on + strand, within EX31_RS13510 at 135.858 kb on - strand, within EX31_RS13510 at 135.884 kb on + strand, within EX31_RS13510 at 135.884 kb on + strand, within EX31_RS13510 at 136.217 kb on + strand, within EX31_RS13510 at 136.217 kb on + strand, within EX31_RS13510 at 136.217 kb on + strand, within EX31_RS13510 at 136.218 kb on - strand, within EX31_RS13510
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 134,637 + -0.1 134,638 - -0.5 134,638 - +0.1 134,638 - -1.1 134,638 - +0.1 134,649 + +0.6 134,649 + +0.5 134,649 + -0.5 134,649 + -0.1 134,649 + -0.9 134,649 + -0.1 134,650 - -0.7 134,650 - +1.0 134,650 - -0.8 134,682 - -0.4 134,683 + +0.5 134,683 + -0.4 134,683 + +0.5 134,684 - -0.3 134,731 + -0.9 134,731 + +0.1 134,732 - +0.5 134,732 - -0.3 134,732 - -0.9 134,732 - -0.1 134,778 + EX31_RS13505 0.17 -0.4 134,778 + EX31_RS13505 0.17 +1.1 134,779 - EX31_RS13505 0.17 +0.5 134,865 + EX31_RS13505 0.33 +0.1 134,866 - EX31_RS13505 0.34 -0.3 134,866 - EX31_RS13505 0.34 -0.2 134,942 - EX31_RS13505 0.48 +0.5 134,998 + EX31_RS13505 0.58 +1.8 134,998 + EX31_RS13505 0.58 +0.5 134,999 - EX31_RS13505 0.59 +1.1 134,999 - EX31_RS13505 0.59 +0.9 134,999 - EX31_RS13505 0.59 +0.4 135,166 + -0.1 135,167 - -0.1 135,186 + -0.1 135,186 + -0.9 135,187 - +0.2 135,230 + +0.5 135,230 + -0.5 135,230 + +0.9 135,249 - +1.3 135,251 - +1.0 135,251 - +0.6 135,251 - +0.5 135,278 + -1.3 135,278 + -0.8 135,278 + +0.4 135,278 + +0.2 135,278 + +0.6 135,278 + -0.4 135,278 + +0.1 135,279 - +0.3 135,279 - +0.9 135,279 - -0.2 135,279 - -0.3 135,279 - +0.5 135,279 - +0.2 135,279 - +1.5 135,279 - -0.1 135,289 + +1.0 135,289 + +0.5 135,289 + +0.7 135,289 + -0.1 135,289 + +0.4 135,289 + -0.4 135,289 + -0.5 135,289 + +1.5 135,289 + +0.7 135,289 + +0.5 135,289 + +0.6 135,289 + -0.7 135,289 + +0.1 135,289 + -0.3 135,289 + +0.6 135,290 - -0.1 135,290 - -0.1 135,290 - -0.2 135,290 - -0.1 135,290 - -0.2 135,290 - +0.3 135,290 - -1.3 135,290 - -2.7 135,290 - +1.5 135,290 - -1.1 135,290 - -1.4 135,290 - +1.0 135,290 - +0.3 135,290 - +0.3 135,324 + +0.9 135,325 - +0.1 135,364 + +0.3 135,364 + +0.9 135,364 + -0.4 135,402 - -0.3 135,413 + +0.2 135,413 + -0.5 135,414 - +0.4 135,414 - +0.2 135,414 - +1.0 135,414 - +0.5 135,463 - +0.1 135,469 + -0.2 135,480 + +1.0 135,481 - +0.6 135,492 + -0.5 135,492 + +0.6 135,492 + +1.5 135,493 - +0.4 135,493 - +0.4 135,528 + EX31_RS13510 0.13 -0.1 135,528 + EX31_RS13510 0.13 +2.8 135,528 + EX31_RS13510 0.13 +0.2 135,529 - EX31_RS13510 0.13 -0.7 135,529 - EX31_RS13510 0.13 -0.5 135,529 - EX31_RS13510 0.13 -0.1 135,529 - EX31_RS13510 0.13 +0.6 135,529 - EX31_RS13510 0.13 -0.2 135,533 + EX31_RS13510 0.14 -0.7 135,533 + EX31_RS13510 0.14 -0.0 135,550 + EX31_RS13510 0.16 +0.7 135,575 + EX31_RS13510 0.18 -0.6 135,699 + EX31_RS13510 0.31 -0.4 135,742 - EX31_RS13510 0.36 +0.6 135,742 - EX31_RS13510 0.36 -0.1 135,742 - EX31_RS13510 0.36 -1.7 135,742 - EX31_RS13510 0.36 -0.0 135,765 + EX31_RS13510 0.38 -1.3 135,840 + EX31_RS13510 0.46 +0.3 135,841 - EX31_RS13510 0.46 +0.1 135,857 + EX31_RS13510 0.48 -0.8 135,857 + EX31_RS13510 0.48 +1.1 135,858 - EX31_RS13510 0.48 +1.0 135,884 + EX31_RS13510 0.51 -0.2 135,884 + EX31_RS13510 0.51 +0.5 136,217 + EX31_RS13510 0.86 +1.1 136,217 + EX31_RS13510 0.86 -0.8 136,217 + EX31_RS13510 0.86 -0.2 136,218 - EX31_RS13510 0.86 +0.8
Or see this region's nucleotide sequence