Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10100 and EX31_RS10105 are separated by 96 nucleotides EX31_RS10105 and EX31_RS10110 are separated by 148 nucleotides EX31_RS10110 and EX31_RS10115 are separated by 43 nucleotides
EX31_RS10100: EX31_RS10100 - two-component system response regulator RssB, at 230,384 to 231,397
_RS10100
EX31_RS10105: EX31_RS10105 - patatin-like phospholipase RssA, at 231,494 to 232,405
_RS10105
EX31_RS10110: EX31_RS10110 - YchJ family protein, at 232,554 to 233,018
_RS10110
EX31_RS10115: EX31_RS10115 - formyltetrahydrofolate deformylase, at 233,062 to 233,910
_RS10115
Position (kb) 231
232
233 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 230.553 kb on - strand, within EX31_RS10100 at 230.595 kb on + strand, within EX31_RS10100 at 230.596 kb on - strand, within EX31_RS10100 at 230.646 kb on - strand, within EX31_RS10100 at 230.701 kb on + strand, within EX31_RS10100 at 230.701 kb on + strand, within EX31_RS10100 at 230.701 kb on + strand, within EX31_RS10100 at 230.705 kb on + strand, within EX31_RS10100 at 230.797 kb on - strand, within EX31_RS10100 at 231.173 kb on + strand, within EX31_RS10100 at 231.296 kb on - strand at 231.317 kb on + strand at 231.317 kb on + strand at 231.317 kb on + strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.318 kb on - strand at 231.370 kb on + strand at 231.371 kb on - strand at 231.382 kb on + strand at 231.383 kb on - strand at 231.423 kb on + strand at 231.423 kb on + strand at 231.423 kb on + strand at 231.424 kb on - strand at 231.424 kb on - strand at 231.424 kb on - strand at 231.424 kb on - strand at 231.424 kb on - strand at 231.427 kb on - strand at 231.487 kb on - strand at 231.701 kb on - strand, within EX31_RS10105 at 231.701 kb on - strand, within EX31_RS10105 at 231.765 kb on - strand, within EX31_RS10105 at 231.821 kb on + strand, within EX31_RS10105 at 231.952 kb on - strand, within EX31_RS10105 at 231.952 kb on - strand, within EX31_RS10105 at 231.952 kb on - strand, within EX31_RS10105 at 232.204 kb on - strand, within EX31_RS10105 at 232.229 kb on + strand, within EX31_RS10105 at 232.230 kb on - strand, within EX31_RS10105 at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.416 kb on + strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.417 kb on - strand at 232.418 kb on + strand at 232.419 kb on - strand at 232.419 kb on - strand at 232.419 kb on - strand at 232.427 kb on + strand at 232.427 kb on + strand at 232.427 kb on + strand at 232.428 kb on - strand at 232.433 kb on + strand at 232.434 kb on - strand at 232.475 kb on - strand at 232.478 kb on + strand at 232.478 kb on + strand at 232.479 kb on - strand at 232.479 kb on - strand at 232.479 kb on - strand at 232.483 kb on + strand at 232.484 kb on - strand at 232.529 kb on + strand at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.611 kb on + strand, within EX31_RS10110 at 232.612 kb on - strand, within EX31_RS10110 at 232.612 kb on - strand, within EX31_RS10110 at 232.626 kb on - strand, within EX31_RS10110 at 232.665 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.674 kb on + strand, within EX31_RS10110 at 232.695 kb on + strand, within EX31_RS10110 at 232.696 kb on - strand, within EX31_RS10110 at 232.751 kb on - strand, within EX31_RS10110 at 232.786 kb on + strand, within EX31_RS10110 at 232.786 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.874 kb on + strand, within EX31_RS10110 at 232.875 kb on - strand, within EX31_RS10110 at 232.875 kb on - strand, within EX31_RS10110 at 232.926 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.929 kb on + strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.930 kb on - strand, within EX31_RS10110 at 232.931 kb on + strand, within EX31_RS10110 at 232.931 kb on + strand, within EX31_RS10110 at 232.943 kb on + strand, within EX31_RS10110 at 232.997 kb on + strand at 232.998 kb on - strand at 232.998 kb on - strand at 233.016 kb on + strand at 233.016 kb on + strand at 233.017 kb on - strand at 233.027 kb on + strand at 233.027 kb on + strand at 233.078 kb on + strand at 233.090 kb on + strand at 233.094 kb on - strand at 233.142 kb on + strand at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.166 kb on + strand, within EX31_RS10115 at 233.214 kb on + strand, within EX31_RS10115
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 230,553 - EX31_RS10100 0.17 -0.1 230,595 + EX31_RS10100 0.21 +0.5 230,596 - EX31_RS10100 0.21 +0.5 230,646 - EX31_RS10100 0.26 -1.3 230,701 + EX31_RS10100 0.31 +1.5 230,701 + EX31_RS10100 0.31 -0.5 230,701 + EX31_RS10100 0.31 -1.7 230,705 + EX31_RS10100 0.32 +0.5 230,797 - EX31_RS10100 0.41 -0.1 231,173 + EX31_RS10100 0.78 -0.4 231,296 - -1.1 231,317 + -1.8 231,317 + -1.4 231,317 + -0.1 231,318 - -1.5 231,318 - -0.5 231,318 - -0.5 231,318 - -0.7 231,318 - +1.5 231,318 - -1.8 231,318 - -1.1 231,370 + -0.8 231,371 - -1.3 231,382 + -0.1 231,383 - -0.1 231,423 + -1.3 231,423 + -0.1 231,423 + -0.3 231,424 - -0.4 231,424 - +0.2 231,424 - +1.3 231,424 - +3.3 231,424 - -0.6 231,427 - -0.5 231,487 - +0.0 231,701 - EX31_RS10105 0.23 +0.1 231,701 - EX31_RS10105 0.23 -0.3 231,765 - EX31_RS10105 0.30 +0.5 231,821 + EX31_RS10105 0.36 -1.4 231,952 - EX31_RS10105 0.50 -1.7 231,952 - EX31_RS10105 0.50 -0.8 231,952 - EX31_RS10105 0.50 -0.6 232,204 - EX31_RS10105 0.78 -0.8 232,229 + EX31_RS10105 0.81 -0.1 232,230 - EX31_RS10105 0.81 -1.1 232,416 + +1.5 232,416 + -0.7 232,416 + -0.5 232,416 + -0.1 232,416 + +0.5 232,416 + -2.8 232,416 + +0.4 232,416 + -1.2 232,416 + +0.2 232,416 + -0.8 232,417 - +0.8 232,417 - -1.8 232,417 - +0.3 232,417 - -0.6 232,417 - -0.3 232,417 - +0.3 232,417 - +1.0 232,417 - -0.3 232,417 - +0.5 232,417 - -0.4 232,417 - +1.1 232,417 - -1.4 232,418 + -0.8 232,419 - -0.5 232,419 - +1.0 232,419 - +0.3 232,427 + +1.5 232,427 + +4.3 232,427 + +1.3 232,428 - +1.5 232,433 + -0.6 232,434 - +0.8 232,475 - +0.6 232,478 + -0.1 232,478 + -2.1 232,479 - +1.2 232,479 - +1.0 232,479 - +1.5 232,483 + +1.5 232,484 - -0.2 232,529 + -0.2 232,611 + EX31_RS10110 0.12 +0.2 232,611 + EX31_RS10110 0.12 -0.5 232,611 + EX31_RS10110 0.12 -2.1 232,611 + EX31_RS10110 0.12 -0.1 232,611 + EX31_RS10110 0.12 +0.9 232,612 - EX31_RS10110 0.12 +0.9 232,612 - EX31_RS10110 0.12 +0.1 232,626 - EX31_RS10110 0.15 +0.4 232,665 + EX31_RS10110 0.24 -0.9 232,674 + EX31_RS10110 0.26 -0.1 232,674 + EX31_RS10110 0.26 -0.6 232,674 + EX31_RS10110 0.26 -0.0 232,695 + EX31_RS10110 0.30 -0.8 232,696 - EX31_RS10110 0.31 +1.3 232,751 - EX31_RS10110 0.42 +0.3 232,786 + EX31_RS10110 0.50 -1.2 232,786 + EX31_RS10110 0.50 +0.9 232,874 + EX31_RS10110 0.69 -0.3 232,874 + EX31_RS10110 0.69 -0.5 232,874 + EX31_RS10110 0.69 +0.3 232,874 + EX31_RS10110 0.69 -0.3 232,875 - EX31_RS10110 0.69 +0.5 232,875 - EX31_RS10110 0.69 -2.5 232,926 + EX31_RS10110 0.80 -1.2 232,929 + EX31_RS10110 0.81 +0.9 232,929 + EX31_RS10110 0.81 +0.8 232,929 + EX31_RS10110 0.81 -0.5 232,929 + EX31_RS10110 0.81 -0.9 232,930 - EX31_RS10110 0.81 -1.9 232,930 - EX31_RS10110 0.81 -1.5 232,930 - EX31_RS10110 0.81 -0.5 232,930 - EX31_RS10110 0.81 -1.4 232,931 + EX31_RS10110 0.81 +0.1 232,931 + EX31_RS10110 0.81 +0.4 232,943 + EX31_RS10110 0.84 +0.2 232,997 + +1.9 232,998 - +0.1 232,998 - -1.4 233,016 + -0.7 233,016 + -0.3 233,017 - -0.1 233,027 + -1.8 233,027 + +0.9 233,078 + -0.7 233,090 + -1.8 233,094 - +1.1 233,142 + -1.4 233,163 + EX31_RS10115 0.12 -1.1 233,163 + EX31_RS10115 0.12 -1.4 233,163 + EX31_RS10115 0.12 -0.4 233,163 + EX31_RS10115 0.12 +1.1 233,164 - EX31_RS10115 0.12 -1.5 233,164 - EX31_RS10115 0.12 -0.1 233,166 + EX31_RS10115 0.12 -1.1 233,214 + EX31_RS10115 0.18 -1.4
Or see this region's nucleotide sequence