Strain Fitness in Rahnella sp. WP5 around EX31_RS10040

Experiment: L-Serine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS10035 and EX31_RS10040 are separated by 80 nucleotidesEX31_RS10040 and EX31_RS10045 are separated by 181 nucleotides EX31_RS10035: EX31_RS10035 - cardiolipin synthase, at 213,041 to 214,501 _RS10035 EX31_RS10040: EX31_RS10040 - YciU family protein, at 214,582 to 214,905 _RS10040 EX31_RS10045: EX31_RS10045 - ABC transporter ATP-binding protein, at 215,087 to 216,124 _RS10045 Position (kb) 214 215Strain fitness (log2 ratio) -2 -1 0 1 2at 213.737 kb on + strand, within EX31_RS10035at 213.737 kb on + strand, within EX31_RS10035at 214.000 kb on + strand, within EX31_RS10035at 214.067 kb on + strand, within EX31_RS10035at 214.500 kb on - strandat 214.522 kb on + strandat 214.522 kb on + strandat 214.522 kb on + strandat 214.525 kb on + strandat 214.525 kb on + strandat 214.525 kb on + strandat 214.581 kb on + strandat 214.582 kb on - strandat 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.726 kb on + strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.828 kb on - strand, within EX31_RS10040at 214.828 kb on - strand, within EX31_RS10040at 214.836 kb on + strand, within EX31_RS10040at 214.903 kb on + strandat 214.904 kb on - strandat 214.984 kb on + strandat 214.985 kb on - strandat 214.985 kb on - strandat 214.995 kb on + strandat 214.995 kb on + strandat 214.995 kb on + strandat 215.082 kb on - strandat 215.216 kb on - strand, within EX31_RS10045at 215.229 kb on - strand, within EX31_RS10045at 215.314 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.376 kb on + strand, within EX31_RS10045at 215.376 kb on + strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.408 kb on + strand, within EX31_RS10045at 215.646 kb on - strand, within EX31_RS10045at 215.699 kb on - strand, within EX31_RS10045at 215.700 kb on + strand, within EX31_RS10045at 215.701 kb on - strand, within EX31_RS10045at 215.822 kb on - strand, within EX31_RS10045

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking
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213,737 + EX31_RS10035 0.48 +1.8
213,737 + EX31_RS10035 0.48 +0.9
214,000 + EX31_RS10035 0.66 +2.1
214,067 + EX31_RS10035 0.70 +0.5
214,500 - +0.9
214,522 + -1.3
214,522 + -0.1
214,522 + -0.3
214,525 + +0.8
214,525 + +0.8
214,525 + -0.4
214,581 + +1.1
214,582 - +1.0
214,724 + EX31_RS10040 0.44 +0.4
214,724 + EX31_RS10040 0.44 -1.5
214,724 + EX31_RS10040 0.44 -0.9
214,724 + EX31_RS10040 0.44 +0.2
214,724 + EX31_RS10040 0.44 +0.9
214,724 + EX31_RS10040 0.44 -0.3
214,726 + EX31_RS10040 0.44 -0.1
214,727 - EX31_RS10040 0.45 -0.8
214,727 - EX31_RS10040 0.45 -0.9
214,727 - EX31_RS10040 0.45 +1.2
214,827 + EX31_RS10040 0.76 +0.5
214,827 + EX31_RS10040 0.76 -1.7
214,827 + EX31_RS10040 0.76 +0.4
214,827 + EX31_RS10040 0.76 -0.4
214,828 - EX31_RS10040 0.76 +1.9
214,828 - EX31_RS10040 0.76 -0.1
214,836 + EX31_RS10040 0.78 -2.2
214,903 + -1.3
214,904 - +1.3
214,984 + -0.3
214,985 - +1.8
214,985 - -1.3
214,995 + -1.0
214,995 + -0.2
214,995 + -0.5
215,082 - -1.1
215,216 - EX31_RS10045 0.12 -0.8
215,229 - EX31_RS10045 0.14 -0.2
215,314 - EX31_RS10045 0.22 -0.1
215,347 - EX31_RS10045 0.25 -0.2
215,347 - EX31_RS10045 0.25 -0.5
215,347 - EX31_RS10045 0.25 +0.5
215,376 + EX31_RS10045 0.28 +0.9
215,376 + EX31_RS10045 0.28 +1.1
215,377 - EX31_RS10045 0.28 -0.1
215,377 - EX31_RS10045 0.28 +0.5
215,377 - EX31_RS10045 0.28 +0.2
215,408 + EX31_RS10045 0.31 +1.1
215,646 - EX31_RS10045 0.54 -0.4
215,699 - EX31_RS10045 0.59 -1.3
215,700 + EX31_RS10045 0.59 -0.1
215,701 - EX31_RS10045 0.59 +0.7
215,822 - EX31_RS10045 0.71 -1.1

Or see this region's nucleotide sequence