Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04535 and EX31_RS04540 are separated by 33 nucleotides EX31_RS04540 and EX31_RS04545 are separated by 297 nucleotides
EX31_RS04535: EX31_RS04535 - DUF1116 domain-containing protein, at 16,447 to 17,715
_RS04535
EX31_RS04540: EX31_RS04540 - acyl-CoA synthetase FdrA, at 17,749 to 19,416
_RS04540
EX31_RS04545: EX31_RS04545 - ureidoglycolate dehydrogenase, at 19,714 to 20,763
_RS04545
Position (kb) 17
18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 16.848 kb on - strand, within EX31_RS04535 at 16.875 kb on + strand, within EX31_RS04535 at 16.876 kb on - strand, within EX31_RS04535 at 16.876 kb on - strand, within EX31_RS04535 at 17.046 kb on + strand, within EX31_RS04535 at 17.046 kb on + strand, within EX31_RS04535 at 17.047 kb on - strand, within EX31_RS04535 at 17.119 kb on - strand, within EX31_RS04535 at 17.119 kb on - strand, within EX31_RS04535 at 17.176 kb on - strand, within EX31_RS04535 at 17.176 kb on - strand, within EX31_RS04535 at 17.176 kb on - strand, within EX31_RS04535 at 17.297 kb on + strand, within EX31_RS04535 at 17.298 kb on - strand, within EX31_RS04535 at 17.466 kb on + strand, within EX31_RS04535 at 17.466 kb on + strand, within EX31_RS04535 at 17.467 kb on - strand, within EX31_RS04535 at 17.467 kb on - strand, within EX31_RS04535 at 17.715 kb on + strand at 17.764 kb on + strand at 17.765 kb on - strand at 17.813 kb on + strand at 17.814 kb on - strand at 17.937 kb on + strand, within EX31_RS04540 at 17.937 kb on + strand, within EX31_RS04540 at 17.938 kb on - strand, within EX31_RS04540 at 17.938 kb on - strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.941 kb on + strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 17.942 kb on - strand, within EX31_RS04540 at 18.008 kb on - strand, within EX31_RS04540 at 18.579 kb on + strand, within EX31_RS04540 at 18.580 kb on - strand, within EX31_RS04540 at 18.585 kb on + strand, within EX31_RS04540 at 18.585 kb on + strand, within EX31_RS04540 at 18.585 kb on + strand, within EX31_RS04540 at 18.585 kb on + strand, within EX31_RS04540 at 18.969 kb on + strand, within EX31_RS04540 at 18.970 kb on - strand, within EX31_RS04540 at 19.031 kb on + strand, within EX31_RS04540 at 19.031 kb on + strand, within EX31_RS04540 at 19.150 kb on + strand, within EX31_RS04540 at 19.150 kb on + strand, within EX31_RS04540 at 19.151 kb on - strand, within EX31_RS04540 at 19.174 kb on - strand, within EX31_RS04540 at 19.185 kb on + strand, within EX31_RS04540 at 19.185 kb on + strand, within EX31_RS04540 at 19.186 kb on - strand, within EX31_RS04540 at 19.227 kb on + strand, within EX31_RS04540 at 19.227 kb on + strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.228 kb on - strand, within EX31_RS04540 at 19.279 kb on + strand at 19.279 kb on + strand at 19.279 kb on + strand at 19.279 kb on + strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.280 kb on - strand at 19.283 kb on - strand at 19.283 kb on - strand at 19.291 kb on - strand at 19.318 kb on + strand at 19.318 kb on + strand at 19.318 kb on + strand at 19.319 kb on - strand at 19.319 kb on - strand at 19.359 kb on + strand at 19.360 kb on - strand at 19.401 kb on + strand at 19.465 kb on - strand at 19.465 kb on - strand at 19.501 kb on - strand at 19.516 kb on + strand at 19.535 kb on - strand at 19.701 kb on + strand at 19.702 kb on - strand at 19.736 kb on + strand at 19.737 kb on - strand at 19.737 kb on - strand at 19.737 kb on - strand at 19.737 kb on - strand at 19.737 kb on - strand at 19.747 kb on - strand at 19.816 kb on + strand at 19.817 kb on - strand at 19.817 kb on - strand at 19.817 kb on - strand at 19.817 kb on - strand at 19.817 kb on - strand at 19.817 kb on - strand at 19.864 kb on + strand, within EX31_RS04545 at 19.864 kb on + strand, within EX31_RS04545 at 19.864 kb on + strand, within EX31_RS04545 at 19.864 kb on + strand, within EX31_RS04545 at 19.867 kb on + strand, within EX31_RS04545 at 19.868 kb on - strand, within EX31_RS04545 at 19.878 kb on + strand, within EX31_RS04545 at 19.878 kb on + strand, within EX31_RS04545 at 19.878 kb on + strand, within EX31_RS04545 at 19.878 kb on + strand, within EX31_RS04545 at 19.879 kb on - strand, within EX31_RS04545 at 20.077 kb on + strand, within EX31_RS04545 at 20.077 kb on + strand, within EX31_RS04545 at 20.078 kb on - strand, within EX31_RS04545 at 20.144 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.170 kb on + strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.171 kb on - strand, within EX31_RS04545 at 20.173 kb on + strand, within EX31_RS04545 at 20.173 kb on + strand, within EX31_RS04545 at 20.173 kb on + strand, within EX31_RS04545 at 20.174 kb on - strand, within EX31_RS04545 at 20.174 kb on - strand, within EX31_RS04545 at 20.315 kb on + strand, within EX31_RS04545 at 20.316 kb on - strand, within EX31_RS04545 at 20.347 kb on + strand, within EX31_RS04545 at 20.348 kb on - strand, within EX31_RS04545
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 16,848 - EX31_RS04535 0.32 -1.3 16,875 + EX31_RS04535 0.34 -0.3 16,876 - EX31_RS04535 0.34 +0.7 16,876 - EX31_RS04535 0.34 +0.5 17,046 + EX31_RS04535 0.47 -0.4 17,046 + EX31_RS04535 0.47 +0.3 17,047 - EX31_RS04535 0.47 +0.7 17,119 - EX31_RS04535 0.53 -1.3 17,119 - EX31_RS04535 0.53 -0.1 17,176 - EX31_RS04535 0.57 -0.0 17,176 - EX31_RS04535 0.57 +0.1 17,176 - EX31_RS04535 0.57 -0.3 17,297 + EX31_RS04535 0.67 -0.1 17,298 - EX31_RS04535 0.67 +0.8 17,466 + EX31_RS04535 0.80 +0.4 17,466 + EX31_RS04535 0.80 +0.3 17,467 - EX31_RS04535 0.80 -0.7 17,467 - EX31_RS04535 0.80 -0.0 17,715 + +0.4 17,764 + +0.9 17,765 - -0.5 17,813 + -0.9 17,814 - +1.2 17,937 + EX31_RS04540 0.11 +0.1 17,937 + EX31_RS04540 0.11 +0.4 17,938 - EX31_RS04540 0.11 +0.1 17,938 - EX31_RS04540 0.11 +1.0 17,941 + EX31_RS04540 0.12 +0.5 17,941 + EX31_RS04540 0.12 -1.1 17,941 + EX31_RS04540 0.12 +0.8 17,941 + EX31_RS04540 0.12 +0.9 17,941 + EX31_RS04540 0.12 +0.1 17,941 + EX31_RS04540 0.12 -0.4 17,941 + EX31_RS04540 0.12 -0.3 17,941 + EX31_RS04540 0.12 +0.6 17,941 + EX31_RS04540 0.12 -0.5 17,941 + EX31_RS04540 0.12 -0.5 17,941 + EX31_RS04540 0.12 -0.8 17,941 + EX31_RS04540 0.12 +0.1 17,941 + EX31_RS04540 0.12 +0.7 17,941 + EX31_RS04540 0.12 +0.3 17,941 + EX31_RS04540 0.12 -0.4 17,941 + EX31_RS04540 0.12 +0.1 17,941 + EX31_RS04540 0.12 +0.7 17,941 + EX31_RS04540 0.12 +0.4 17,941 + EX31_RS04540 0.12 +0.0 17,941 + EX31_RS04540 0.12 +2.0 17,941 + EX31_RS04540 0.12 +1.1 17,941 + EX31_RS04540 0.12 +0.0 17,941 + EX31_RS04540 0.12 -0.4 17,942 - EX31_RS04540 0.12 -0.3 17,942 - EX31_RS04540 0.12 +0.2 17,942 - EX31_RS04540 0.12 +0.4 17,942 - EX31_RS04540 0.12 -0.1 17,942 - EX31_RS04540 0.12 +0.4 17,942 - EX31_RS04540 0.12 +1.0 17,942 - EX31_RS04540 0.12 -0.2 17,942 - EX31_RS04540 0.12 +0.7 17,942 - EX31_RS04540 0.12 +0.9 17,942 - EX31_RS04540 0.12 +0.1 17,942 - EX31_RS04540 0.12 +1.0 17,942 - EX31_RS04540 0.12 +0.9 17,942 - EX31_RS04540 0.12 +0.2 17,942 - EX31_RS04540 0.12 +0.4 17,942 - EX31_RS04540 0.12 -0.8 17,942 - EX31_RS04540 0.12 -0.2 17,942 - EX31_RS04540 0.12 +1.6 17,942 - EX31_RS04540 0.12 -0.8 17,942 - EX31_RS04540 0.12 +1.1 17,942 - EX31_RS04540 0.12 +1.0 17,942 - EX31_RS04540 0.12 +0.2 17,942 - EX31_RS04540 0.12 +0.3 17,942 - EX31_RS04540 0.12 +0.2 17,942 - EX31_RS04540 0.12 -0.1 18,008 - EX31_RS04540 0.16 -2.1 18,579 + EX31_RS04540 0.50 -0.8 18,580 - EX31_RS04540 0.50 +0.7 18,585 + EX31_RS04540 0.50 +1.1 18,585 + EX31_RS04540 0.50 +0.8 18,585 + EX31_RS04540 0.50 +0.8 18,585 + EX31_RS04540 0.50 -0.4 18,969 + EX31_RS04540 0.73 +0.3 18,970 - EX31_RS04540 0.73 +0.4 19,031 + EX31_RS04540 0.77 -0.5 19,031 + EX31_RS04540 0.77 +1.0 19,150 + EX31_RS04540 0.84 -0.2 19,150 + EX31_RS04540 0.84 +0.7 19,151 - EX31_RS04540 0.84 +0.2 19,174 - EX31_RS04540 0.85 +0.2 19,185 + EX31_RS04540 0.86 +0.3 19,185 + EX31_RS04540 0.86 +0.4 19,186 - EX31_RS04540 0.86 -0.7 19,227 + EX31_RS04540 0.89 +0.2 19,227 + EX31_RS04540 0.89 -0.2 19,228 - EX31_RS04540 0.89 -0.5 19,228 - EX31_RS04540 0.89 +0.4 19,228 - EX31_RS04540 0.89 +0.2 19,228 - EX31_RS04540 0.89 +0.8 19,228 - EX31_RS04540 0.89 -0.1 19,228 - EX31_RS04540 0.89 -2.4 19,279 + +0.6 19,279 + -0.1 19,279 + +0.5 19,279 + +0.9 19,280 - -0.3 19,280 - -1.3 19,280 - -0.6 19,280 - -0.4 19,280 - +0.2 19,280 - +0.5 19,280 - -0.6 19,280 - +0.4 19,283 - -0.9 19,283 - +0.2 19,291 - +1.2 19,318 + -0.1 19,318 + +0.7 19,318 + -1.0 19,319 - +0.6 19,319 - +0.9 19,359 + +0.3 19,360 - +1.1 19,401 + -0.3 19,465 - -0.4 19,465 - +0.3 19,501 - -0.1 19,516 + +0.2 19,535 - -0.1 19,701 + -0.0 19,702 - -0.0 19,736 + -0.2 19,737 - +0.3 19,737 - +1.4 19,737 - +0.6 19,737 - -0.1 19,737 - -0.6 19,747 - +0.6 19,816 + -0.9 19,817 - +0.5 19,817 - -2.0 19,817 - -0.9 19,817 - +0.8 19,817 - +0.4 19,817 - -0.3 19,864 + EX31_RS04545 0.14 -0.8 19,864 + EX31_RS04545 0.14 -0.0 19,864 + EX31_RS04545 0.14 -0.9 19,864 + EX31_RS04545 0.14 -0.5 19,867 + EX31_RS04545 0.15 -0.0 19,868 - EX31_RS04545 0.15 +1.0 19,878 + EX31_RS04545 0.16 +0.9 19,878 + EX31_RS04545 0.16 -0.3 19,878 + EX31_RS04545 0.16 +0.6 19,878 + EX31_RS04545 0.16 -1.5 19,879 - EX31_RS04545 0.16 +0.1 20,077 + EX31_RS04545 0.35 +0.8 20,077 + EX31_RS04545 0.35 +1.9 20,078 - EX31_RS04545 0.35 -0.2 20,144 + EX31_RS04545 0.41 +0.1 20,170 + EX31_RS04545 0.43 +0.4 20,170 + EX31_RS04545 0.43 -0.2 20,170 + EX31_RS04545 0.43 -0.6 20,170 + EX31_RS04545 0.43 -0.4 20,170 + EX31_RS04545 0.43 +1.0 20,170 + EX31_RS04545 0.43 -0.1 20,171 - EX31_RS04545 0.44 +1.0 20,171 - EX31_RS04545 0.44 -0.2 20,171 - EX31_RS04545 0.44 -1.1 20,171 - EX31_RS04545 0.44 +0.2 20,171 - EX31_RS04545 0.44 +0.3 20,171 - EX31_RS04545 0.44 -0.3 20,173 + EX31_RS04545 0.44 -1.4 20,173 + EX31_RS04545 0.44 -0.7 20,173 + EX31_RS04545 0.44 -0.4 20,174 - EX31_RS04545 0.44 -0.4 20,174 - EX31_RS04545 0.44 +0.3 20,315 + EX31_RS04545 0.57 -0.0 20,316 - EX31_RS04545 0.57 -0.6 20,347 + EX31_RS04545 0.60 -0.1 20,348 - EX31_RS04545 0.60 -0.1
Or see this region's nucleotide sequence