Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03685 and EX31_RS03690 are separated by 1 nucleotides EX31_RS03690 and EX31_RS03695 are separated by 10 nucleotides EX31_RS03695 and EX31_RS03700 are separated by 283 nucleotides
EX31_RS03685: EX31_RS03685 - 3-isopropylmalate dehydrogenase, at 236,982 to 238,073
_RS03685
EX31_RS03690: EX31_RS03690 - 3-isopropylmalate dehydratase large subunit, at 238,075 to 239,475
_RS03690
EX31_RS03695: EX31_RS03695 - 3-isopropylmalate dehydratase small subunit, at 239,486 to 240,088
_RS03695
EX31_RS03700: EX31_RS03700 - hypothetical protein, at 240,372 to 241,064
_RS03700
Position (kb) 238
239
240 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 237.266 kb on - strand, within EX31_RS03685 at 237.296 kb on + strand, within EX31_RS03685 at 237.296 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.322 kb on - strand, within EX31_RS03685 at 237.410 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.484 kb on - strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.683 kb on + strand, within EX31_RS03685 at 237.684 kb on - strand, within EX31_RS03685 at 237.858 kb on - strand, within EX31_RS03685 at 237.905 kb on - strand, within EX31_RS03685 at 237.913 kb on - strand, within EX31_RS03685 at 237.962 kb on + strand, within EX31_RS03685 at 237.963 kb on - strand, within EX31_RS03685 at 237.963 kb on - strand, within EX31_RS03685 at 237.997 kb on + strand at 237.997 kb on + strand at 237.997 kb on + strand at 237.998 kb on - strand at 237.998 kb on - strand at 237.998 kb on - strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.072 kb on - strand at 238.072 kb on - strand at 238.072 kb on - strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.148 kb on + strand at 238.149 kb on - strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.239 kb on + strand, within EX31_RS03690 at 238.281 kb on + strand, within EX31_RS03690 at 238.282 kb on - strand, within EX31_RS03690 at 238.282 kb on - strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.367 kb on - strand, within EX31_RS03690 at 238.367 kb on - strand, within EX31_RS03690 at 238.385 kb on - strand, within EX31_RS03690 at 238.563 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.621 kb on - strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.723 kb on - strand, within EX31_RS03690 at 238.723 kb on - strand, within EX31_RS03690 at 238.789 kb on - strand, within EX31_RS03690 at 238.789 kb on - strand, within EX31_RS03690 at 238.906 kb on - strand, within EX31_RS03690 at 238.906 kb on - strand, within EX31_RS03690 at 238.947 kb on + strand, within EX31_RS03690 at 238.948 kb on - strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.070 kb on + strand, within EX31_RS03690 at 239.094 kb on + strand, within EX31_RS03690 at 239.095 kb on - strand, within EX31_RS03690 at 239.186 kb on + strand, within EX31_RS03690 at 239.187 kb on - strand, within EX31_RS03690 at 239.187 kb on - strand, within EX31_RS03690 at 239.250 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.298 kb on + strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.299 kb on - strand, within EX31_RS03690 at 239.352 kb on + strand at 239.353 kb on - strand at 239.353 kb on - strand at 239.364 kb on + strand at 239.365 kb on - strand at 239.365 kb on - strand at 239.492 kb on - strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.537 kb on - strand at 239.563 kb on + strand, within EX31_RS03695 at 239.575 kb on - strand, within EX31_RS03695 at 239.684 kb on + strand, within EX31_RS03695 at 239.685 kb on - strand, within EX31_RS03695 at 239.769 kb on - strand, within EX31_RS03695 at 239.814 kb on - strand, within EX31_RS03695 at 239.897 kb on - strand, within EX31_RS03695 at 239.961 kb on - strand, within EX31_RS03695 at 240.046 kb on + strand at 240.047 kb on - strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.093 kb on + strand at 240.093 kb on + strand at 240.093 kb on + strand at 240.175 kb on - strand at 240.184 kb on + strand at 240.184 kb on + strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.218 kb on - strand at 240.218 kb on - strand at 240.218 kb on - strand at 240.227 kb on - strand at 240.227 kb on - strand at 240.307 kb on - strand at 240.325 kb on - strand at 240.437 kb on - strand at 240.446 kb on + strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 237,266 - EX31_RS03685 0.26 -3.5 237,296 + EX31_RS03685 0.29 -2.7 237,296 + EX31_RS03685 0.29 -2.2 237,299 + EX31_RS03685 0.29 -2.5 237,299 + EX31_RS03685 0.29 -2.0 237,299 + EX31_RS03685 0.29 -3.0 237,322 - EX31_RS03685 0.31 -2.7 237,410 + EX31_RS03685 0.39 -3.5 237,414 + EX31_RS03685 0.40 -2.4 237,414 + EX31_RS03685 0.40 -3.0 237,414 + EX31_RS03685 0.40 -2.0 237,414 + EX31_RS03685 0.40 -1.0 237,414 + EX31_RS03685 0.40 -2.8 237,415 - EX31_RS03685 0.40 -2.8 237,415 - EX31_RS03685 0.40 -0.6 237,415 - EX31_RS03685 0.40 -2.6 237,415 - EX31_RS03685 0.40 -1.9 237,415 - EX31_RS03685 0.40 +0.2 237,415 - EX31_RS03685 0.40 -2.2 237,480 + EX31_RS03685 0.46 -0.9 237,480 + EX31_RS03685 0.46 -3.0 237,480 + EX31_RS03685 0.46 -2.4 237,480 + EX31_RS03685 0.46 -2.5 237,480 + EX31_RS03685 0.46 -1.8 237,481 - EX31_RS03685 0.46 +0.2 237,481 - EX31_RS03685 0.46 -2.2 237,481 - EX31_RS03685 0.46 -4.1 237,484 - EX31_RS03685 0.46 -1.4 237,590 + EX31_RS03685 0.56 -3.0 237,590 + EX31_RS03685 0.56 -3.2 237,590 + EX31_RS03685 0.56 -3.3 237,590 + EX31_RS03685 0.56 -2.5 237,591 - EX31_RS03685 0.56 -3.6 237,591 - EX31_RS03685 0.56 -1.3 237,591 - EX31_RS03685 0.56 -1.7 237,591 - EX31_RS03685 0.56 -2.9 237,591 - EX31_RS03685 0.56 -1.3 237,654 + EX31_RS03685 0.62 -2.4 237,654 + EX31_RS03685 0.62 -2.0 237,654 + EX31_RS03685 0.62 -2.6 237,654 + EX31_RS03685 0.62 -3.6 237,654 + EX31_RS03685 0.62 -2.5 237,654 + EX31_RS03685 0.62 -1.0 237,654 + EX31_RS03685 0.62 -4.4 237,654 + EX31_RS03685 0.62 -2.9 237,654 + EX31_RS03685 0.62 -2.6 237,654 + EX31_RS03685 0.62 -3.0 237,654 + EX31_RS03685 0.62 -2.1 237,654 + EX31_RS03685 0.62 -3.1 237,654 + EX31_RS03685 0.62 -2.9 237,654 + EX31_RS03685 0.62 -2.6 237,654 + EX31_RS03685 0.62 +0.3 237,654 + EX31_RS03685 0.62 -3.5 237,654 + EX31_RS03685 0.62 -2.9 237,654 + EX31_RS03685 0.62 -2.3 237,655 - EX31_RS03685 0.62 -1.9 237,655 - EX31_RS03685 0.62 -4.2 237,655 - EX31_RS03685 0.62 -1.6 237,655 - EX31_RS03685 0.62 +2.2 237,655 - EX31_RS03685 0.62 -1.4 237,655 - EX31_RS03685 0.62 -2.0 237,655 - EX31_RS03685 0.62 -1.0 237,655 - EX31_RS03685 0.62 -0.6 237,655 - EX31_RS03685 0.62 -0.6 237,655 - EX31_RS03685 0.62 -3.0 237,655 - EX31_RS03685 0.62 -1.6 237,655 - EX31_RS03685 0.62 -2.9 237,655 - EX31_RS03685 0.62 -1.2 237,655 - EX31_RS03685 0.62 -1.0 237,655 - EX31_RS03685 0.62 +0.2 237,683 + EX31_RS03685 0.64 -2.6 237,684 - EX31_RS03685 0.64 -2.4 237,858 - EX31_RS03685 0.80 -2.5 237,905 - EX31_RS03685 0.85 -3.5 237,913 - EX31_RS03685 0.85 -1.8 237,962 + EX31_RS03685 0.90 -3.2 237,963 - EX31_RS03685 0.90 -2.3 237,963 - EX31_RS03685 0.90 -3.4 237,997 + -0.6 237,997 + -3.2 237,997 + -3.9 237,998 - -2.8 237,998 - -3.5 237,998 - -2.8 238,071 + -3.5 238,071 + -1.0 238,071 + -2.8 238,071 + -3.7 238,072 - -3.0 238,072 - -3.7 238,072 - -3.1 238,115 + -2.9 238,115 + -2.3 238,115 + -2.4 238,115 + -3.0 238,115 + -3.2 238,115 + -2.0 238,115 + -3.2 238,115 + -0.8 238,115 + -2.0 238,116 - -1.4 238,116 - -1.7 238,116 - -3.3 238,116 - -3.5 238,116 - -0.4 238,116 - -1.9 238,148 + -2.5 238,149 - -0.6 238,150 + -3.3 238,150 + -2.6 238,150 + -2.9 238,150 + -3.5 238,150 + -2.0 238,150 + -3.0 238,150 + -3.8 238,150 + -4.8 238,150 + -2.4 238,150 + -2.1 238,150 + -2.1 238,150 + -1.5 238,151 - -2.3 238,151 - -2.7 238,151 - -3.1 238,151 - -1.4 238,151 - -2.5 238,151 - -3.0 238,151 - -2.2 238,151 - -3.2 238,151 - -2.6 238,239 + EX31_RS03690 0.12 -2.1 238,281 + EX31_RS03690 0.15 -3.2 238,282 - EX31_RS03690 0.15 -3.6 238,282 - EX31_RS03690 0.15 -1.7 238,366 + EX31_RS03690 0.21 -2.0 238,366 + EX31_RS03690 0.21 -3.1 238,366 + EX31_RS03690 0.21 -2.8 238,367 - EX31_RS03690 0.21 -2.0 238,367 - EX31_RS03690 0.21 -3.6 238,385 - EX31_RS03690 0.22 -1.9 238,563 + EX31_RS03690 0.35 -3.1 238,620 + EX31_RS03690 0.39 -2.5 238,620 + EX31_RS03690 0.39 -3.5 238,620 + EX31_RS03690 0.39 -0.6 238,620 + EX31_RS03690 0.39 -2.4 238,621 - EX31_RS03690 0.39 -2.7 238,722 + EX31_RS03690 0.46 -1.0 238,722 + EX31_RS03690 0.46 -2.4 238,722 + EX31_RS03690 0.46 -1.7 238,723 - EX31_RS03690 0.46 -2.2 238,723 - EX31_RS03690 0.46 -1.0 238,789 - EX31_RS03690 0.51 -2.2 238,789 - EX31_RS03690 0.51 -0.4 238,906 - EX31_RS03690 0.59 -2.1 238,906 - EX31_RS03690 0.59 -2.3 238,947 + EX31_RS03690 0.62 -3.1 238,948 - EX31_RS03690 0.62 -2.2 239,047 + EX31_RS03690 0.69 -2.9 239,047 + EX31_RS03690 0.69 -3.4 239,047 + EX31_RS03690 0.69 -5.0 239,047 + EX31_RS03690 0.69 -1.8 239,047 + EX31_RS03690 0.69 -1.1 239,047 + EX31_RS03690 0.69 -2.3 239,047 + EX31_RS03690 0.69 -2.2 239,048 - EX31_RS03690 0.69 -1.6 239,048 - EX31_RS03690 0.69 -1.9 239,048 - EX31_RS03690 0.69 -3.3 239,048 - EX31_RS03690 0.69 -1.9 239,048 - EX31_RS03690 0.69 -2.2 239,048 - EX31_RS03690 0.69 -3.8 239,048 - EX31_RS03690 0.69 -2.1 239,048 - EX31_RS03690 0.69 +0.8 239,048 - EX31_RS03690 0.69 -2.1 239,048 - EX31_RS03690 0.69 -2.3 239,070 + EX31_RS03690 0.71 -1.0 239,094 + EX31_RS03690 0.73 -2.0 239,095 - EX31_RS03690 0.73 -1.4 239,186 + EX31_RS03690 0.79 -2.4 239,187 - EX31_RS03690 0.79 -3.5 239,187 - EX31_RS03690 0.79 -2.9 239,250 + EX31_RS03690 0.84 -3.6 239,298 + EX31_RS03690 0.87 -2.0 239,298 + EX31_RS03690 0.87 -2.8 239,298 + EX31_RS03690 0.87 -2.5 239,298 + EX31_RS03690 0.87 -1.7 239,298 + EX31_RS03690 0.87 -1.0 239,298 + EX31_RS03690 0.87 -2.0 239,298 + EX31_RS03690 0.87 -3.4 239,298 + EX31_RS03690 0.87 -2.8 239,298 + EX31_RS03690 0.87 -1.4 239,298 + EX31_RS03690 0.87 -2.8 239,298 + EX31_RS03690 0.87 -2.8 239,298 + EX31_RS03690 0.87 -0.4 239,299 - EX31_RS03690 0.87 -3.0 239,299 - EX31_RS03690 0.87 -0.1 239,299 - EX31_RS03690 0.87 -1.4 239,299 - EX31_RS03690 0.87 -2.5 239,299 - EX31_RS03690 0.87 -2.0 239,299 - EX31_RS03690 0.87 -2.5 239,299 - EX31_RS03690 0.87 -3.4 239,299 - EX31_RS03690 0.87 -1.6 239,299 - EX31_RS03690 0.87 -2.1 239,352 + -2.0 239,353 - -1.4 239,353 - -2.1 239,364 + -3.7 239,365 - -3.7 239,365 - -2.5 239,492 - -2.7 239,536 + -1.8 239,536 + -2.6 239,536 + -2.8 239,536 + -0.1 239,537 - -3.0 239,563 + EX31_RS03695 0.13 -3.9 239,575 - EX31_RS03695 0.15 -3.0 239,684 + EX31_RS03695 0.33 -2.9 239,685 - EX31_RS03695 0.33 -1.9 239,769 - EX31_RS03695 0.47 -2.5 239,814 - EX31_RS03695 0.54 -2.9 239,897 - EX31_RS03695 0.68 -3.3 239,961 - EX31_RS03695 0.79 -2.2 240,046 + -1.3 240,047 - -2.2 240,090 + -0.3 240,090 + +0.4 240,090 + +0.4 240,090 + -1.4 240,091 - -0.4 240,091 - -1.5 240,091 - -0.5 240,091 - +0.1 240,093 + -0.7 240,093 + -0.0 240,093 + +0.7 240,175 - +0.2 240,184 + +0.0 240,184 + -0.3 240,185 - +0.0 240,185 - -0.1 240,185 - -0.5 240,185 - +0.0 240,218 - +0.3 240,218 - +0.5 240,218 - -1.2 240,227 - +0.1 240,227 - +2.0 240,307 - +0.0 240,325 - +0.5 240,437 - +0.2 240,446 + EX31_RS03700 0.11 +1.0 240,456 - EX31_RS03700 0.12 -0.6 240,456 - EX31_RS03700 0.12 +0.0 240,456 - EX31_RS03700 0.12 -0.6
Or see this region's nucleotide sequence