Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25095 and EX31_RS25910 are separated by 31 nucleotides EX31_RS25910 and EX31_RS25100 are separated by 204 nucleotides EX31_RS25100 and EX31_RS01930 are separated by 81 nucleotides EX31_RS01930 and EX31_RS01935 overlap by 17 nucleotides
EX31_RS25095: EX31_RS25095 - hypothetical protein, at 417,723 to 418,470
_RS25095
EX31_RS25910: EX31_RS25910 - hypothetical protein, at 418,502 to 418,747
_RS25910
EX31_RS25100: EX31_RS25100 - PAAR domain-containing protein, at 418,952 to 419,218
_RS25100
EX31_RS01930: EX31_RS01930 - type VI secretion protein, at 419,300 to 420,385
_RS01930
EX31_RS01935: EX31_RS01935 - type VI secretion protein VasK, at 420,369 to 423,776
_RS01935
Position (kb) 419
420
421 Strain fitness (log2 ratio) -2
-1
0
1
2 at 418.580 kb on - strand, within EX31_RS25910 at 418.585 kb on - strand, within EX31_RS25910 at 418.881 kb on + strand at 418.882 kb on - strand at 418.887 kb on + strand at 418.892 kb on + strand at 418.923 kb on + strand at 419.005 kb on + strand, within EX31_RS25100 at 419.148 kb on + strand, within EX31_RS25100 at 419.148 kb on + strand, within EX31_RS25100 at 419.148 kb on + strand, within EX31_RS25100 at 419.149 kb on - strand, within EX31_RS25100 at 419.211 kb on + strand at 419.212 kb on - strand at 419.216 kb on + strand at 419.216 kb on + strand at 419.217 kb on - strand at 419.217 kb on - strand at 419.248 kb on + strand at 419.289 kb on + strand at 419.374 kb on - strand at 419.377 kb on + strand at 419.377 kb on + strand at 419.377 kb on + strand at 419.377 kb on + strand at 419.377 kb on + strand at 419.378 kb on - strand at 419.403 kb on + strand at 419.444 kb on + strand, within EX31_RS01930 at 419.444 kb on + strand, within EX31_RS01930 at 419.528 kb on + strand, within EX31_RS01930 at 419.529 kb on - strand, within EX31_RS01930 at 419.530 kb on + strand, within EX31_RS01930 at 419.530 kb on + strand, within EX31_RS01930 at 419.531 kb on - strand, within EX31_RS01930 at 419.569 kb on + strand, within EX31_RS01930 at 419.569 kb on + strand, within EX31_RS01930 at 419.583 kb on + strand, within EX31_RS01930 at 419.586 kb on + strand, within EX31_RS01930 at 419.642 kb on + strand, within EX31_RS01930 at 419.653 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.671 kb on + strand, within EX31_RS01930 at 419.686 kb on + strand, within EX31_RS01930 at 419.687 kb on - strand, within EX31_RS01930 at 419.721 kb on + strand, within EX31_RS01930 at 419.722 kb on - strand, within EX31_RS01930 at 419.739 kb on + strand, within EX31_RS01930 at 419.739 kb on + strand, within EX31_RS01930 at 419.740 kb on - strand, within EX31_RS01930 at 419.899 kb on + strand, within EX31_RS01930 at 419.923 kb on - strand, within EX31_RS01930 at 419.977 kb on + strand, within EX31_RS01930 at 420.006 kb on + strand, within EX31_RS01930 at 420.035 kb on - strand, within EX31_RS01930 at 420.076 kb on - strand, within EX31_RS01930 at 420.160 kb on + strand, within EX31_RS01930 at 420.160 kb on + strand, within EX31_RS01930 at 420.160 kb on + strand, within EX31_RS01930 at 420.206 kb on - strand, within EX31_RS01930 at 420.224 kb on + strand, within EX31_RS01930 at 420.224 kb on + strand, within EX31_RS01930 at 420.224 kb on + strand, within EX31_RS01930 at 420.224 kb on + strand, within EX31_RS01930 at 420.224 kb on + strand, within EX31_RS01930 at 420.225 kb on - strand, within EX31_RS01930 at 420.225 kb on - strand, within EX31_RS01930 at 420.225 kb on - strand, within EX31_RS01930 at 420.225 kb on - strand, within EX31_RS01930 at 420.225 kb on - strand, within EX31_RS01930 at 420.344 kb on + strand at 420.345 kb on - strand at 420.376 kb on + strand at 420.377 kb on - strand at 420.377 kb on - strand at 420.379 kb on + strand at 420.379 kb on + strand at 420.379 kb on + strand at 420.379 kb on + strand at 420.380 kb on - strand at 420.380 kb on - strand at 420.380 kb on - strand at 420.383 kb on + strand at 420.383 kb on + strand at 420.431 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.449 kb on + strand at 420.450 kb on - strand at 420.450 kb on - strand at 420.450 kb on - strand at 420.451 kb on + strand at 420.512 kb on + strand at 420.513 kb on - strand at 420.523 kb on + strand at 420.790 kb on - strand, within EX31_RS01935 at 420.814 kb on + strand, within EX31_RS01935 at 420.817 kb on + strand, within EX31_RS01935 at 420.817 kb on + strand, within EX31_RS01935 at 420.817 kb on + strand, within EX31_RS01935 at 420.818 kb on - strand, within EX31_RS01935 at 420.820 kb on + strand, within EX31_RS01935 at 421.038 kb on + strand, within EX31_RS01935 at 421.038 kb on + strand, within EX31_RS01935 at 421.038 kb on + strand, within EX31_RS01935 at 421.038 kb on + strand, within EX31_RS01935 at 421.039 kb on - strand, within EX31_RS01935 at 421.039 kb on - strand, within EX31_RS01935 at 421.039 kb on - strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.042 kb on + strand, within EX31_RS01935 at 421.043 kb on - strand, within EX31_RS01935 at 421.043 kb on - strand, within EX31_RS01935 at 421.073 kb on + strand, within EX31_RS01935 at 421.073 kb on + strand, within EX31_RS01935 at 421.121 kb on + strand, within EX31_RS01935 at 421.137 kb on + strand, within EX31_RS01935 at 421.138 kb on - strand, within EX31_RS01935 at 421.138 kb on - strand, within EX31_RS01935 at 421.340 kb on + strand, within EX31_RS01935 at 421.340 kb on + strand, within EX31_RS01935 at 421.341 kb on - strand, within EX31_RS01935 at 421.341 kb on - strand, within EX31_RS01935
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 418,580 - EX31_RS25910 0.32 +1.5 418,585 - EX31_RS25910 0.34 +0.8 418,881 + +1.6 418,882 - +1.2 418,887 + +0.9 418,892 + -1.1 418,923 + +1.1 419,005 + EX31_RS25100 0.20 -0.5 419,148 + EX31_RS25100 0.73 -0.1 419,148 + EX31_RS25100 0.73 +0.9 419,148 + EX31_RS25100 0.73 -1.0 419,149 - EX31_RS25100 0.74 -0.7 419,211 + -0.7 419,212 - +0.9 419,216 + -0.5 419,216 + -0.4 419,217 - -0.7 419,217 - -1.5 419,248 + +2.3 419,289 + +0.2 419,374 - -0.1 419,377 + -0.7 419,377 + -0.7 419,377 + +0.9 419,377 + +0.2 419,377 + -0.0 419,378 - -0.4 419,403 + -0.2 419,444 + EX31_RS01930 0.13 +0.9 419,444 + EX31_RS01930 0.13 -1.3 419,528 + EX31_RS01930 0.21 +1.3 419,529 - EX31_RS01930 0.21 +0.9 419,530 + EX31_RS01930 0.21 +1.1 419,530 + EX31_RS01930 0.21 -1.5 419,531 - EX31_RS01930 0.21 -0.5 419,569 + EX31_RS01930 0.25 +0.4 419,569 + EX31_RS01930 0.25 +1.9 419,583 + EX31_RS01930 0.26 -1.8 419,586 + EX31_RS01930 0.26 -0.2 419,642 + EX31_RS01930 0.31 +0.9 419,653 + EX31_RS01930 0.33 -1.2 419,671 + EX31_RS01930 0.34 +1.4 419,671 + EX31_RS01930 0.34 -1.1 419,671 + EX31_RS01930 0.34 -0.7 419,671 + EX31_RS01930 0.34 -0.4 419,671 + EX31_RS01930 0.34 -1.8 419,671 + EX31_RS01930 0.34 -1.0 419,686 + EX31_RS01930 0.36 -1.4 419,687 - EX31_RS01930 0.36 +0.3 419,721 + EX31_RS01930 0.39 -0.0 419,722 - EX31_RS01930 0.39 +0.3 419,739 + EX31_RS01930 0.40 +1.0 419,739 + EX31_RS01930 0.40 +0.5 419,740 - EX31_RS01930 0.41 +0.4 419,899 + EX31_RS01930 0.55 +0.2 419,923 - EX31_RS01930 0.57 -1.2 419,977 + EX31_RS01930 0.62 -0.1 420,006 + EX31_RS01930 0.65 -1.0 420,035 - EX31_RS01930 0.68 -0.4 420,076 - EX31_RS01930 0.71 +0.7 420,160 + EX31_RS01930 0.79 -0.5 420,160 + EX31_RS01930 0.79 +0.8 420,160 + EX31_RS01930 0.79 -0.2 420,206 - EX31_RS01930 0.83 +0.1 420,224 + EX31_RS01930 0.85 -1.3 420,224 + EX31_RS01930 0.85 -2.2 420,224 + EX31_RS01930 0.85 -0.4 420,224 + EX31_RS01930 0.85 -1.1 420,224 + EX31_RS01930 0.85 -0.2 420,225 - EX31_RS01930 0.85 +0.4 420,225 - EX31_RS01930 0.85 -0.9 420,225 - EX31_RS01930 0.85 -1.4 420,225 - EX31_RS01930 0.85 -0.2 420,225 - EX31_RS01930 0.85 +1.1 420,344 + +0.4 420,345 - +0.3 420,376 + +0.5 420,377 - +0.7 420,377 - -0.4 420,379 + -2.1 420,379 + -1.9 420,379 + +0.1 420,379 + +1.3 420,380 - +0.2 420,380 - +1.9 420,380 - +0.7 420,383 + +0.0 420,383 + -2.5 420,431 + -0.7 420,449 + +0.1 420,449 + -0.4 420,449 + -1.5 420,449 + -1.1 420,449 + +1.9 420,449 + +1.6 420,449 + +1.9 420,449 + +0.4 420,449 + +0.3 420,449 + -0.1 420,449 + +0.3 420,449 + -0.7 420,450 - -1.2 420,450 - -0.4 420,450 - -1.4 420,451 + -0.7 420,512 + +1.4 420,513 - +0.9 420,523 + +1.4 420,790 - EX31_RS01935 0.12 -0.2 420,814 + EX31_RS01935 0.13 -2.5 420,817 + EX31_RS01935 0.13 +0.5 420,817 + EX31_RS01935 0.13 +1.4 420,817 + EX31_RS01935 0.13 -0.9 420,818 - EX31_RS01935 0.13 -1.1 420,820 + EX31_RS01935 0.13 +1.9 421,038 + EX31_RS01935 0.20 +0.3 421,038 + EX31_RS01935 0.20 -0.3 421,038 + EX31_RS01935 0.20 +0.4 421,038 + EX31_RS01935 0.20 -1.5 421,039 - EX31_RS01935 0.20 -1.2 421,039 - EX31_RS01935 0.20 -0.3 421,039 - EX31_RS01935 0.20 -0.7 421,042 + EX31_RS01935 0.20 -2.0 421,042 + EX31_RS01935 0.20 -0.2 421,042 + EX31_RS01935 0.20 +0.4 421,042 + EX31_RS01935 0.20 +1.0 421,042 + EX31_RS01935 0.20 +0.9 421,042 + EX31_RS01935 0.20 +1.2 421,042 + EX31_RS01935 0.20 -0.7 421,042 + EX31_RS01935 0.20 +0.9 421,043 - EX31_RS01935 0.20 +0.2 421,043 - EX31_RS01935 0.20 -0.1 421,073 + EX31_RS01935 0.21 -0.4 421,073 + EX31_RS01935 0.21 +0.1 421,121 + EX31_RS01935 0.22 +0.3 421,137 + EX31_RS01935 0.23 +0.3 421,138 - EX31_RS01935 0.23 +1.5 421,138 - EX31_RS01935 0.23 -1.1 421,340 + EX31_RS01935 0.28 -0.9 421,340 + EX31_RS01935 0.28 +0.5 421,341 - EX31_RS01935 0.29 +0.3 421,341 - EX31_RS01935 0.29 -0.5
Or see this region's nucleotide sequence