Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01630 and EX31_RS01635 overlap by 4 nucleotides EX31_RS01635 and EX31_RS01640 overlap by 4 nucleotides EX31_RS01640 and EX31_RS01645 overlap by 1 nucleotides EX31_RS01645 and EX31_RS01650 are separated by 393 nucleotides
EX31_RS01630: EX31_RS01630 - phage portal protein, at 361,800 to 363,278
_RS01630
EX31_RS01635: EX31_RS01635 - hypothetical protein, at 363,275 to 363,493
_RS01635
EX31_RS01640: EX31_RS01640 - phage terminase large subunit family protein, at 363,490 to 365,592
_RS01640
EX31_RS01645: EX31_RS01645 - DUF1441 family protein, at 365,592 to 366,080
_RS01645
EX31_RS01650: EX31_RS01650 - hypothetical protein, at 366,474 to 366,851
_RS01650
Position (kb) 363
364
365
366 Strain fitness (log2 ratio) -2
-1
0
1
2 at 362.628 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.675 kb on - strand, within EX31_RS01630 at 362.704 kb on + strand, within EX31_RS01630 at 362.704 kb on + strand, within EX31_RS01630 at 362.704 kb on + strand, within EX31_RS01630 at 362.704 kb on + strand, within EX31_RS01630 at 362.705 kb on - strand, within EX31_RS01630 at 362.705 kb on - strand, within EX31_RS01630 at 362.756 kb on - strand, within EX31_RS01630 at 362.957 kb on + strand, within EX31_RS01630 at 363.194 kb on - strand at 363.454 kb on + strand, within EX31_RS01635 at 363.454 kb on + strand, within EX31_RS01635 at 363.454 kb on + strand, within EX31_RS01635 at 363.454 kb on + strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.455 kb on - strand, within EX31_RS01635 at 363.456 kb on + strand, within EX31_RS01635 at 363.456 kb on + strand, within EX31_RS01635 at 363.456 kb on + strand, within EX31_RS01635 at 363.456 kb on + strand, within EX31_RS01635 at 363.457 kb on - strand, within EX31_RS01635 at 363.457 kb on - strand, within EX31_RS01635 at 363.457 kb on - strand, within EX31_RS01635 at 363.569 kb on - strand at 363.569 kb on - strand at 363.576 kb on + strand at 363.577 kb on - strand at 363.677 kb on + strand at 363.707 kb on + strand, within EX31_RS01640 at 363.707 kb on + strand, within EX31_RS01640 at 363.709 kb on + strand, within EX31_RS01640 at 363.709 kb on + strand, within EX31_RS01640 at 363.782 kb on + strand, within EX31_RS01640 at 363.782 kb on + strand, within EX31_RS01640 at 363.783 kb on - strand, within EX31_RS01640 at 363.783 kb on - strand, within EX31_RS01640 at 363.833 kb on + strand, within EX31_RS01640 at 363.834 kb on - strand, within EX31_RS01640 at 363.834 kb on - strand, within EX31_RS01640 at 363.834 kb on - strand, within EX31_RS01640 at 363.834 kb on - strand, within EX31_RS01640 at 363.834 kb on - strand, within EX31_RS01640 at 363.888 kb on + strand, within EX31_RS01640 at 364.016 kb on + strand, within EX31_RS01640 at 364.017 kb on - strand, within EX31_RS01640 at 364.064 kb on + strand, within EX31_RS01640 at 364.064 kb on + strand, within EX31_RS01640 at 364.065 kb on - strand, within EX31_RS01640 at 364.393 kb on - strand, within EX31_RS01640 at 364.422 kb on + strand, within EX31_RS01640 at 364.464 kb on + strand, within EX31_RS01640 at 364.465 kb on - strand, within EX31_RS01640 at 364.465 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.467 kb on - strand, within EX31_RS01640 at 364.554 kb on + strand, within EX31_RS01640 at 364.555 kb on - strand, within EX31_RS01640 at 364.555 kb on - strand, within EX31_RS01640 at 364.556 kb on + strand, within EX31_RS01640 at 364.556 kb on + strand, within EX31_RS01640 at 364.881 kb on + strand, within EX31_RS01640 at 364.883 kb on + strand, within EX31_RS01640 at 364.883 kb on + strand, within EX31_RS01640 at 364.883 kb on + strand, within EX31_RS01640 at 364.884 kb on - strand, within EX31_RS01640 at 364.885 kb on + strand, within EX31_RS01640 at 364.885 kb on + strand, within EX31_RS01640 at 364.885 kb on + strand, within EX31_RS01640 at 364.886 kb on - strand, within EX31_RS01640 at 364.998 kb on + strand, within EX31_RS01640 at 364.998 kb on + strand, within EX31_RS01640 at 364.998 kb on + strand, within EX31_RS01640 at 364.998 kb on + strand, within EX31_RS01640 at 364.999 kb on - strand, within EX31_RS01640 at 364.999 kb on - strand, within EX31_RS01640 at 364.999 kb on - strand, within EX31_RS01640 at 364.999 kb on - strand, within EX31_RS01640 at 365.073 kb on - strand, within EX31_RS01640 at 365.094 kb on + strand, within EX31_RS01640 at 365.094 kb on + strand, within EX31_RS01640 at 365.095 kb on - strand, within EX31_RS01640 at 365.095 kb on - strand, within EX31_RS01640 at 365.139 kb on + strand, within EX31_RS01640 at 365.139 kb on + strand, within EX31_RS01640 at 365.139 kb on + strand, within EX31_RS01640 at 365.140 kb on - strand, within EX31_RS01640 at 365.145 kb on - strand, within EX31_RS01640 at 365.145 kb on - strand, within EX31_RS01640 at 365.149 kb on - strand, within EX31_RS01640 at 365.236 kb on - strand, within EX31_RS01640 at 365.324 kb on + strand, within EX31_RS01640 at 365.325 kb on - strand, within EX31_RS01640 at 365.325 kb on - strand, within EX31_RS01640 at 365.359 kb on - strand, within EX31_RS01640 at 365.396 kb on + strand at 365.422 kb on - strand at 365.435 kb on + strand at 365.436 kb on - strand at 365.493 kb on + strand at 365.493 kb on + strand at 365.493 kb on + strand at 365.493 kb on + strand at 365.493 kb on + strand at 365.494 kb on - strand at 365.494 kb on - strand at 365.494 kb on - strand at 365.494 kb on - strand at 365.495 kb on + strand at 365.495 kb on + strand at 365.495 kb on + strand at 365.496 kb on - strand at 365.523 kb on + strand at 365.524 kb on - strand at 365.542 kb on + strand at 365.543 kb on - strand at 365.543 kb on - strand at 365.551 kb on + strand at 365.673 kb on - strand, within EX31_RS01645 at 365.679 kb on + strand, within EX31_RS01645 at 365.805 kb on + strand, within EX31_RS01645 at 365.806 kb on - strand, within EX31_RS01645 at 365.806 kb on - strand, within EX31_RS01645 at 365.947 kb on - strand, within EX31_RS01645 at 366.327 kb on + strand at 366.351 kb on - strand at 366.396 kb on - strand at 366.396 kb on - strand at 366.397 kb on + strand at 366.398 kb on - strand at 366.417 kb on + strand at 366.418 kb on - strand at 366.418 kb on - strand at 366.456 kb on + strand at 366.458 kb on + strand at 366.459 kb on - strand at 366.459 kb on - strand at 366.459 kb on - strand at 366.459 kb on - strand at 366.459 kb on - strand at 366.459 kb on - strand at 366.463 kb on - strand at 366.463 kb on - strand at 366.499 kb on + strand at 366.499 kb on + strand at 366.500 kb on - strand at 366.510 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 362,628 - EX31_RS01630 0.56 +1.1 362,675 - EX31_RS01630 0.59 +0.6 362,675 - EX31_RS01630 0.59 -0.4 362,675 - EX31_RS01630 0.59 -0.1 362,675 - EX31_RS01630 0.59 +0.8 362,675 - EX31_RS01630 0.59 -1.5 362,675 - EX31_RS01630 0.59 -0.9 362,704 + EX31_RS01630 0.61 -0.2 362,704 + EX31_RS01630 0.61 +0.7 362,704 + EX31_RS01630 0.61 -1.1 362,704 + EX31_RS01630 0.61 -0.6 362,705 - EX31_RS01630 0.61 +0.5 362,705 - EX31_RS01630 0.61 +0.0 362,756 - EX31_RS01630 0.65 -0.2 362,957 + EX31_RS01630 0.78 +0.7 363,194 - +0.3 363,454 + EX31_RS01635 0.82 -0.7 363,454 + EX31_RS01635 0.82 +1.7 363,454 + EX31_RS01635 0.82 -1.1 363,454 + EX31_RS01635 0.82 +0.4 363,455 - EX31_RS01635 0.82 +1.3 363,455 - EX31_RS01635 0.82 +0.9 363,455 - EX31_RS01635 0.82 +0.6 363,455 - EX31_RS01635 0.82 +0.6 363,455 - EX31_RS01635 0.82 -0.1 363,455 - EX31_RS01635 0.82 +0.2 363,455 - EX31_RS01635 0.82 -0.0 363,455 - EX31_RS01635 0.82 +0.8 363,455 - EX31_RS01635 0.82 +0.2 363,455 - EX31_RS01635 0.82 -1.2 363,456 + EX31_RS01635 0.83 +0.9 363,456 + EX31_RS01635 0.83 +0.1 363,456 + EX31_RS01635 0.83 -1.0 363,456 + EX31_RS01635 0.83 +0.2 363,457 - EX31_RS01635 0.83 -0.5 363,457 - EX31_RS01635 0.83 +0.6 363,457 - EX31_RS01635 0.83 +1.1 363,569 - -1.9 363,569 - +0.3 363,576 + -0.1 363,577 - -1.1 363,677 + -0.8 363,707 + EX31_RS01640 0.10 +0.2 363,707 + EX31_RS01640 0.10 -0.0 363,709 + EX31_RS01640 0.10 -1.2 363,709 + EX31_RS01640 0.10 -1.7 363,782 + EX31_RS01640 0.14 -0.4 363,782 + EX31_RS01640 0.14 +0.5 363,783 - EX31_RS01640 0.14 +0.3 363,783 - EX31_RS01640 0.14 -0.2 363,833 + EX31_RS01640 0.16 +0.0 363,834 - EX31_RS01640 0.16 -0.5 363,834 - EX31_RS01640 0.16 -0.4 363,834 - EX31_RS01640 0.16 -0.1 363,834 - EX31_RS01640 0.16 +0.9 363,834 - EX31_RS01640 0.16 -0.9 363,888 + EX31_RS01640 0.19 -0.2 364,016 + EX31_RS01640 0.25 +0.6 364,017 - EX31_RS01640 0.25 +1.9 364,064 + EX31_RS01640 0.27 -0.4 364,064 + EX31_RS01640 0.27 -0.6 364,065 - EX31_RS01640 0.27 +1.9 364,393 - EX31_RS01640 0.43 -0.3 364,422 + EX31_RS01640 0.44 -1.4 364,464 + EX31_RS01640 0.46 +1.5 364,465 - EX31_RS01640 0.46 -1.8 364,465 - EX31_RS01640 0.46 +0.0 364,467 - EX31_RS01640 0.46 -0.5 364,467 - EX31_RS01640 0.46 +1.1 364,467 - EX31_RS01640 0.46 +1.0 364,467 - EX31_RS01640 0.46 -0.8 364,467 - EX31_RS01640 0.46 +1.3 364,467 - EX31_RS01640 0.46 +0.3 364,554 + EX31_RS01640 0.51 +1.2 364,555 - EX31_RS01640 0.51 +0.0 364,555 - EX31_RS01640 0.51 -1.0 364,556 + EX31_RS01640 0.51 +0.6 364,556 + EX31_RS01640 0.51 -0.7 364,881 + EX31_RS01640 0.66 +0.3 364,883 + EX31_RS01640 0.66 -1.1 364,883 + EX31_RS01640 0.66 -0.9 364,883 + EX31_RS01640 0.66 +0.6 364,884 - EX31_RS01640 0.66 +1.9 364,885 + EX31_RS01640 0.66 -0.4 364,885 + EX31_RS01640 0.66 -1.8 364,885 + EX31_RS01640 0.66 +0.3 364,886 - EX31_RS01640 0.66 -0.8 364,998 + EX31_RS01640 0.72 +1.1 364,998 + EX31_RS01640 0.72 +1.6 364,998 + EX31_RS01640 0.72 +0.9 364,998 + EX31_RS01640 0.72 +0.5 364,999 - EX31_RS01640 0.72 -0.2 364,999 - EX31_RS01640 0.72 -1.1 364,999 - EX31_RS01640 0.72 -1.2 364,999 - EX31_RS01640 0.72 -1.3 365,073 - EX31_RS01640 0.75 +0.1 365,094 + EX31_RS01640 0.76 +1.7 365,094 + EX31_RS01640 0.76 -0.3 365,095 - EX31_RS01640 0.76 +0.7 365,095 - EX31_RS01640 0.76 -0.4 365,139 + EX31_RS01640 0.78 -0.3 365,139 + EX31_RS01640 0.78 -2.0 365,139 + EX31_RS01640 0.78 +0.3 365,140 - EX31_RS01640 0.78 -0.1 365,145 - EX31_RS01640 0.79 -1.1 365,145 - EX31_RS01640 0.79 -0.4 365,149 - EX31_RS01640 0.79 +0.3 365,236 - EX31_RS01640 0.83 -0.7 365,324 + EX31_RS01640 0.87 -1.4 365,325 - EX31_RS01640 0.87 +0.1 365,325 - EX31_RS01640 0.87 -1.1 365,359 - EX31_RS01640 0.89 +0.6 365,396 + -1.5 365,422 - +0.9 365,435 + +0.5 365,436 - +1.2 365,493 + +0.9 365,493 + -1.8 365,493 + -1.2 365,493 + -0.1 365,493 + +0.2 365,494 - -0.4 365,494 - +0.4 365,494 - +1.1 365,494 - +0.3 365,495 + -0.4 365,495 + +0.1 365,495 + +0.7 365,496 - +0.3 365,523 + +0.3 365,524 - -0.2 365,542 + +0.9 365,543 - -0.9 365,543 - +0.5 365,551 + -0.7 365,673 - EX31_RS01645 0.17 +0.2 365,679 + EX31_RS01645 0.18 +0.5 365,805 + EX31_RS01645 0.44 -1.2 365,806 - EX31_RS01645 0.44 -0.8 365,806 - EX31_RS01645 0.44 +0.7 365,947 - EX31_RS01645 0.73 -0.5 366,327 + +0.5 366,351 - -0.4 366,396 - +0.1 366,396 - -0.7 366,397 + +1.1 366,398 - +1.3 366,417 + +0.4 366,418 - +1.5 366,418 - -1.2 366,456 + +1.5 366,458 + -2.1 366,459 - -1.9 366,459 - +1.2 366,459 - -0.7 366,459 - -0.7 366,459 - -2.2 366,459 - -0.2 366,463 - -0.8 366,463 - -0.2 366,499 + +0.2 366,499 + +0.7 366,500 - +0.2 366,510 - +1.3
Or see this region's nucleotide sequence