Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22805 and EX31_RS22810 are separated by 343 nucleotides EX31_RS22810 and EX31_RS22815 are separated by 70 nucleotides EX31_RS22815 and EX31_RS22820 are separated by 56 nucleotides
EX31_RS22805: EX31_RS22805 - multidrug efflux RND transporter permease AcrD, at 320,188 to 323,343
_RS22805
EX31_RS22810: EX31_RS22810 - helix-turn-helix domain-containing protein, at 323,687 to 323,977
_RS22810
EX31_RS22815: EX31_RS22815 - GDP-mannose pyrophosphatase NudK, at 324,048 to 324,638
_RS22815
EX31_RS22820: EX31_RS22820 - hypothetical protein, at 324,695 to 325,870
_RS22820
Position (kb) 324
325 Strain fitness (log2 ratio) -2
-1
0
1
2 at 323.085 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.114 kb on + strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.115 kb on - strand at 323.166 kb on + strand at 323.166 kb on + strand at 323.174 kb on - strand at 323.198 kb on + strand at 323.198 kb on + strand at 323.241 kb on - strand at 323.377 kb on + strand at 323.378 kb on - strand at 323.381 kb on + strand at 323.381 kb on + strand at 323.381 kb on + strand at 323.381 kb on + strand at 323.382 kb on - strand at 323.382 kb on - strand at 323.382 kb on - strand at 323.382 kb on - strand at 323.410 kb on - strand at 323.438 kb on - strand at 323.438 kb on - strand at 323.494 kb on + strand at 323.494 kb on + strand at 323.495 kb on - strand at 323.507 kb on + strand at 323.507 kb on + strand at 323.508 kb on - strand at 323.508 kb on - strand at 323.508 kb on - strand at 323.508 kb on - strand at 323.508 kb on - strand at 323.508 kb on - strand at 323.522 kb on + strand at 323.523 kb on - strand at 323.523 kb on - strand at 323.523 kb on - strand at 323.539 kb on + strand at 323.540 kb on - strand at 323.554 kb on + strand at 323.555 kb on - strand at 323.563 kb on + strand at 323.563 kb on + strand at 323.563 kb on + strand at 323.563 kb on + strand at 323.564 kb on - strand at 323.564 kb on - strand at 323.585 kb on + strand at 323.585 kb on + strand at 323.585 kb on + strand at 323.595 kb on + strand at 323.663 kb on + strand at 323.663 kb on + strand at 323.663 kb on + strand at 323.663 kb on + strand at 323.663 kb on + strand at 323.663 kb on + strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.664 kb on - strand at 323.767 kb on - strand, within EX31_RS22810 at 323.818 kb on - strand, within EX31_RS22810 at 323.878 kb on + strand, within EX31_RS22810 at 323.956 kb on + strand at 323.956 kb on + strand at 323.957 kb on - strand at 323.995 kb on + strand at 323.995 kb on + strand at 323.995 kb on + strand at 323.996 kb on - strand at 324.005 kb on - strand at 324.047 kb on + strand at 324.047 kb on + strand at 324.048 kb on - strand at 324.098 kb on - strand at 324.098 kb on - strand at 324.098 kb on - strand at 324.114 kb on + strand, within EX31_RS22815 at 324.115 kb on - strand, within EX31_RS22815 at 324.115 kb on - strand, within EX31_RS22815 at 324.130 kb on - strand, within EX31_RS22815 at 324.135 kb on + strand, within EX31_RS22815 at 324.142 kb on + strand, within EX31_RS22815 at 324.152 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.184 kb on + strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.185 kb on - strand, within EX31_RS22815 at 324.186 kb on + strand, within EX31_RS22815 at 324.186 kb on + strand, within EX31_RS22815 at 324.187 kb on - strand, within EX31_RS22815 at 324.187 kb on - strand, within EX31_RS22815 at 324.187 kb on - strand, within EX31_RS22815 at 324.210 kb on - strand, within EX31_RS22815 at 324.219 kb on + strand, within EX31_RS22815 at 324.219 kb on + strand, within EX31_RS22815 at 324.220 kb on - strand, within EX31_RS22815 at 324.220 kb on - strand, within EX31_RS22815 at 324.220 kb on - strand, within EX31_RS22815 at 324.220 kb on - strand, within EX31_RS22815 at 324.220 kb on - strand, within EX31_RS22815 at 324.221 kb on + strand, within EX31_RS22815 at 324.221 kb on + strand, within EX31_RS22815 at 324.221 kb on + strand, within EX31_RS22815 at 324.222 kb on - strand, within EX31_RS22815 at 324.273 kb on + strand, within EX31_RS22815 at 324.273 kb on + strand, within EX31_RS22815 at 324.273 kb on + strand, within EX31_RS22815 at 324.273 kb on + strand, within EX31_RS22815 at 324.274 kb on - strand, within EX31_RS22815 at 324.274 kb on - strand, within EX31_RS22815 at 324.274 kb on - strand, within EX31_RS22815 at 324.274 kb on - strand, within EX31_RS22815 at 324.274 kb on - strand, within EX31_RS22815 at 324.296 kb on + strand, within EX31_RS22815 at 324.296 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.397 kb on + strand, within EX31_RS22815 at 324.398 kb on - strand, within EX31_RS22815 at 324.398 kb on - strand, within EX31_RS22815 at 324.398 kb on - strand, within EX31_RS22815 at 324.398 kb on - strand, within EX31_RS22815 at 324.398 kb on - strand, within EX31_RS22815 at 324.401 kb on + strand, within EX31_RS22815 at 324.401 kb on + strand, within EX31_RS22815 at 324.401 kb on + strand, within EX31_RS22815 at 324.401 kb on + strand, within EX31_RS22815 at 324.402 kb on - strand, within EX31_RS22815 at 324.402 kb on - strand, within EX31_RS22815 at 324.417 kb on + strand, within EX31_RS22815 at 324.417 kb on + strand, within EX31_RS22815 at 324.417 kb on + strand, within EX31_RS22815 at 324.417 kb on + strand, within EX31_RS22815 at 324.417 kb on + strand, within EX31_RS22815 at 324.418 kb on - strand, within EX31_RS22815 at 324.418 kb on - strand, within EX31_RS22815 at 324.418 kb on - strand, within EX31_RS22815 at 324.418 kb on - strand, within EX31_RS22815 at 324.418 kb on - strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.419 kb on + strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.420 kb on - strand, within EX31_RS22815 at 324.596 kb on + strand at 324.596 kb on + strand at 324.596 kb on + strand at 324.596 kb on + strand at 324.596 kb on + strand at 324.596 kb on + strand at 324.596 kb on + strand at 324.597 kb on - strand at 324.597 kb on - strand at 324.597 kb on - strand at 324.609 kb on + strand at 324.609 kb on + strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.691 kb on + strand at 324.691 kb on + strand at 324.813 kb on + strand, within EX31_RS22820 at 324.814 kb on - strand, within EX31_RS22820 at 324.814 kb on - strand, within EX31_RS22820 at 324.835 kb on - strand, within EX31_RS22820 at 324.855 kb on - strand, within EX31_RS22820 at 324.855 kb on - strand, within EX31_RS22820 at 325.038 kb on + strand, within EX31_RS22820 at 325.158 kb on + strand, within EX31_RS22820 at 325.158 kb on + strand, within EX31_RS22820 at 325.158 kb on + strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.159 kb on - strand, within EX31_RS22820 at 325.165 kb on + strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.166 kb on - strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.169 kb on + strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.170 kb on - strand, within EX31_RS22820 at 325.225 kb on - strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.258 kb on + strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.259 kb on - strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.327 kb on + strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.328 kb on - strand, within EX31_RS22820 at 325.458 kb on + strand, within EX31_RS22820 at 325.458 kb on + strand, within EX31_RS22820 at 325.459 kb on - strand, within EX31_RS22820 at 325.630 kb on + strand, within EX31_RS22820
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 323,085 + +0.1 323,114 + -1.1 323,114 + -1.3 323,114 + +1.1 323,114 + +0.7 323,114 + +0.3 323,114 + +0.1 323,114 + -0.2 323,114 + +0.5 323,114 + +0.2 323,114 + -0.1 323,114 + +0.8 323,115 - -1.1 323,115 - -1.4 323,115 - -0.1 323,115 - -0.1 323,115 - -0.1 323,115 - -0.1 323,115 - -1.2 323,115 - -0.7 323,115 - -0.2 323,115 - +0.2 323,166 + -1.2 323,166 + +1.0 323,174 - -0.4 323,198 + +0.0 323,198 + -1.4 323,241 - -0.1 323,377 + +0.4 323,378 - +0.6 323,381 + -0.3 323,381 + -0.6 323,381 + -0.4 323,381 + -1.2 323,382 - -1.3 323,382 - -2.0 323,382 - +0.1 323,382 - +1.0 323,410 - -0.2 323,438 - -0.0 323,438 - +0.5 323,494 + +0.6 323,494 + -0.1 323,495 - +0.1 323,507 + -0.0 323,507 + +0.1 323,508 - +1.0 323,508 - -0.2 323,508 - +0.3 323,508 - -0.3 323,508 - +0.2 323,508 - -0.8 323,522 + -1.6 323,523 - -1.4 323,523 - -1.1 323,523 - -0.1 323,539 + +1.5 323,540 - +0.5 323,554 + +0.9 323,555 - -1.4 323,563 + +0.8 323,563 + +0.5 323,563 + -0.2 323,563 + +0.6 323,564 - +0.8 323,564 - -0.3 323,585 + +1.0 323,585 + -0.3 323,585 + -0.0 323,595 + +0.8 323,663 + -0.8 323,663 + -1.1 323,663 + -0.5 323,663 + -0.2 323,663 + +1.0 323,663 + -0.0 323,664 - +0.2 323,664 - +0.4 323,664 - -0.2 323,664 - -0.6 323,664 - +0.6 323,664 - -1.0 323,664 - -0.4 323,664 - +0.5 323,767 - EX31_RS22810 0.27 +0.9 323,818 - EX31_RS22810 0.45 +0.2 323,878 + EX31_RS22810 0.66 +0.8 323,956 + -2.1 323,956 + -0.7 323,957 - +0.9 323,995 + +0.5 323,995 + +1.0 323,995 + -0.4 323,996 - +2.1 324,005 - -0.5 324,047 + -0.3 324,047 + +1.2 324,048 - -0.2 324,098 - -1.1 324,098 - -0.0 324,098 - +1.9 324,114 + EX31_RS22815 0.11 +1.6 324,115 - EX31_RS22815 0.11 +0.4 324,115 - EX31_RS22815 0.11 -2.2 324,130 - EX31_RS22815 0.14 +0.4 324,135 + EX31_RS22815 0.15 -1.3 324,142 + EX31_RS22815 0.16 +0.5 324,152 + EX31_RS22815 0.18 -0.9 324,184 + EX31_RS22815 0.23 -0.2 324,184 + EX31_RS22815 0.23 +0.1 324,184 + EX31_RS22815 0.23 +0.2 324,184 + EX31_RS22815 0.23 +0.6 324,184 + EX31_RS22815 0.23 +0.4 324,184 + EX31_RS22815 0.23 -0.4 324,184 + EX31_RS22815 0.23 +1.5 324,184 + EX31_RS22815 0.23 +0.6 324,184 + EX31_RS22815 0.23 +0.8 324,184 + EX31_RS22815 0.23 +0.5 324,184 + EX31_RS22815 0.23 -0.0 324,185 - EX31_RS22815 0.23 +1.1 324,185 - EX31_RS22815 0.23 +1.0 324,185 - EX31_RS22815 0.23 -0.7 324,185 - EX31_RS22815 0.23 +0.4 324,185 - EX31_RS22815 0.23 +0.5 324,185 - EX31_RS22815 0.23 +0.7 324,185 - EX31_RS22815 0.23 -0.4 324,185 - EX31_RS22815 0.23 +0.7 324,186 + EX31_RS22815 0.23 +0.9 324,186 + EX31_RS22815 0.23 -0.2 324,187 - EX31_RS22815 0.24 +0.2 324,187 - EX31_RS22815 0.24 +0.9 324,187 - EX31_RS22815 0.24 +0.5 324,210 - EX31_RS22815 0.27 +0.2 324,219 + EX31_RS22815 0.29 -0.1 324,219 + EX31_RS22815 0.29 +0.6 324,220 - EX31_RS22815 0.29 -0.6 324,220 - EX31_RS22815 0.29 +0.7 324,220 - EX31_RS22815 0.29 -0.7 324,220 - EX31_RS22815 0.29 +0.3 324,220 - EX31_RS22815 0.29 +1.4 324,221 + EX31_RS22815 0.29 -0.4 324,221 + EX31_RS22815 0.29 +0.9 324,221 + EX31_RS22815 0.29 -0.8 324,222 - EX31_RS22815 0.29 -0.3 324,273 + EX31_RS22815 0.38 -0.1 324,273 + EX31_RS22815 0.38 -0.4 324,273 + EX31_RS22815 0.38 -0.2 324,273 + EX31_RS22815 0.38 +1.0 324,274 - EX31_RS22815 0.38 -0.8 324,274 - EX31_RS22815 0.38 +1.2 324,274 - EX31_RS22815 0.38 +0.5 324,274 - EX31_RS22815 0.38 +1.4 324,274 - EX31_RS22815 0.38 +1.4 324,296 + EX31_RS22815 0.42 +0.6 324,296 + EX31_RS22815 0.42 -1.2 324,397 + EX31_RS22815 0.59 +0.1 324,397 + EX31_RS22815 0.59 -0.4 324,397 + EX31_RS22815 0.59 +0.5 324,397 + EX31_RS22815 0.59 +0.1 324,397 + EX31_RS22815 0.59 +1.8 324,397 + EX31_RS22815 0.59 -0.2 324,397 + EX31_RS22815 0.59 -0.3 324,398 - EX31_RS22815 0.59 +0.4 324,398 - EX31_RS22815 0.59 +0.3 324,398 - EX31_RS22815 0.59 +0.3 324,398 - EX31_RS22815 0.59 -1.2 324,398 - EX31_RS22815 0.59 +2.7 324,401 + EX31_RS22815 0.60 +0.9 324,401 + EX31_RS22815 0.60 +0.5 324,401 + EX31_RS22815 0.60 -0.0 324,401 + EX31_RS22815 0.60 -0.3 324,402 - EX31_RS22815 0.60 -0.2 324,402 - EX31_RS22815 0.60 -1.2 324,417 + EX31_RS22815 0.62 +0.6 324,417 + EX31_RS22815 0.62 +0.2 324,417 + EX31_RS22815 0.62 -1.1 324,417 + EX31_RS22815 0.62 -0.2 324,417 + EX31_RS22815 0.62 -0.8 324,418 - EX31_RS22815 0.63 -1.9 324,418 - EX31_RS22815 0.63 +0.6 324,418 - EX31_RS22815 0.63 +0.6 324,418 - EX31_RS22815 0.63 -0.4 324,418 - EX31_RS22815 0.63 +0.9 324,419 + EX31_RS22815 0.63 +0.0 324,419 + EX31_RS22815 0.63 -0.2 324,419 + EX31_RS22815 0.63 -1.4 324,419 + EX31_RS22815 0.63 +1.0 324,419 + EX31_RS22815 0.63 -0.3 324,419 + EX31_RS22815 0.63 -0.5 324,419 + EX31_RS22815 0.63 -0.6 324,419 + EX31_RS22815 0.63 +0.2 324,419 + EX31_RS22815 0.63 -0.4 324,420 - EX31_RS22815 0.63 +1.4 324,420 - EX31_RS22815 0.63 +0.2 324,420 - EX31_RS22815 0.63 +0.5 324,420 - EX31_RS22815 0.63 -1.5 324,420 - EX31_RS22815 0.63 -0.0 324,420 - EX31_RS22815 0.63 +0.8 324,420 - EX31_RS22815 0.63 +0.3 324,596 + -0.0 324,596 + +0.3 324,596 + -1.5 324,596 + +1.0 324,596 + -0.1 324,596 + -0.1 324,596 + +1.8 324,597 - -0.9 324,597 - -1.4 324,597 - +0.3 324,609 + +0.9 324,609 + +0.1 324,610 - -1.5 324,610 - +0.8 324,610 - +0.6 324,691 + +0.3 324,691 + -0.6 324,813 + EX31_RS22820 0.10 +0.5 324,814 - EX31_RS22820 0.10 -0.4 324,814 - EX31_RS22820 0.10 -0.2 324,835 - EX31_RS22820 0.12 -1.1 324,855 - EX31_RS22820 0.14 +0.5 324,855 - EX31_RS22820 0.14 -0.5 325,038 + EX31_RS22820 0.29 +0.0 325,158 + EX31_RS22820 0.39 -0.6 325,158 + EX31_RS22820 0.39 -0.2 325,158 + EX31_RS22820 0.39 +0.3 325,159 - EX31_RS22820 0.39 -0.0 325,159 - EX31_RS22820 0.39 +1.4 325,159 - EX31_RS22820 0.39 +0.6 325,165 + EX31_RS22820 0.40 -0.2 325,166 - EX31_RS22820 0.40 +0.7 325,166 - EX31_RS22820 0.40 +0.6 325,166 - EX31_RS22820 0.40 -0.6 325,169 + EX31_RS22820 0.40 -1.1 325,169 + EX31_RS22820 0.40 -1.2 325,169 + EX31_RS22820 0.40 +1.1 325,170 - EX31_RS22820 0.40 -1.3 325,170 - EX31_RS22820 0.40 -0.5 325,170 - EX31_RS22820 0.40 -0.5 325,170 - EX31_RS22820 0.40 -1.2 325,170 - EX31_RS22820 0.40 +0.1 325,170 - EX31_RS22820 0.40 +0.5 325,225 - EX31_RS22820 0.45 -1.3 325,258 + EX31_RS22820 0.48 -0.5 325,258 + EX31_RS22820 0.48 +1.1 325,258 + EX31_RS22820 0.48 +0.0 325,258 + EX31_RS22820 0.48 -0.1 325,259 - EX31_RS22820 0.48 +2.1 325,259 - EX31_RS22820 0.48 +0.3 325,259 - EX31_RS22820 0.48 -1.1 325,259 - EX31_RS22820 0.48 -0.2 325,259 - EX31_RS22820 0.48 -0.5 325,259 - EX31_RS22820 0.48 -0.6 325,259 - EX31_RS22820 0.48 -0.3 325,327 + EX31_RS22820 0.54 -0.9 325,327 + EX31_RS22820 0.54 +0.5 325,327 + EX31_RS22820 0.54 -1.4 325,327 + EX31_RS22820 0.54 -1.8 325,328 - EX31_RS22820 0.54 +0.5 325,328 - EX31_RS22820 0.54 +1.7 325,328 - EX31_RS22820 0.54 -0.0 325,328 - EX31_RS22820 0.54 -0.8 325,328 - EX31_RS22820 0.54 -0.6 325,458 + EX31_RS22820 0.65 +1.3 325,458 + EX31_RS22820 0.65 -0.6 325,459 - EX31_RS22820 0.65 +2.1 325,630 + EX31_RS22820 0.80 +0.7
Or see this region's nucleotide sequence