Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17805 and EX31_RS17810 overlap by 23 nucleotides EX31_RS17810 and EX31_RS17815 overlap by 4 nucleotides EX31_RS17815 and EX31_RS17820 are separated by 27 nucleotides EX31_RS17820 and EX31_RS17825 are separated by 86 nucleotides
EX31_RS17805: EX31_RS17805 - citrate lyase holo-[acyl-carrier protein] synthase, at 117,450 to 117,989
_RS17805
EX31_RS17810: EX31_RS17810 - triphosphoribosyl-dephospho-CoA synthase CitG, at 117,967 to 118,806
_RS17810
EX31_RS17815: EX31_RS17815 - alpha/beta hydrolase, at 118,803 to 119,351
_RS17815
EX31_RS17820: EX31_RS17820 - DUF805 domain-containing protein, at 119,379 to 119,855
_RS17820
EX31_RS17825: EX31_RS17825 - LacI family DNA-binding transcriptional regulator, at 119,942 to 121,012
_RS17825
Position (kb) 118
119
120 Strain fitness (log2 ratio) -2
-1
0
1
2 at 117.947 kb on + strand at 118.275 kb on + strand, within EX31_RS17810 at 118.278 kb on + strand, within EX31_RS17810 at 118.310 kb on + strand, within EX31_RS17810 at 118.310 kb on + strand, within EX31_RS17810 at 118.576 kb on - strand, within EX31_RS17810 at 118.600 kb on + strand, within EX31_RS17810 at 118.600 kb on + strand, within EX31_RS17810 at 118.601 kb on - strand, within EX31_RS17810 at 118.910 kb on + strand, within EX31_RS17815 at 118.912 kb on + strand, within EX31_RS17815 at 118.912 kb on + strand, within EX31_RS17815 at 118.913 kb on - strand, within EX31_RS17815 at 119.258 kb on + strand, within EX31_RS17815 at 119.258 kb on + strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.259 kb on - strand, within EX31_RS17815 at 119.361 kb on - strand at 119.500 kb on + strand, within EX31_RS17820 at 119.500 kb on + strand, within EX31_RS17820 at 119.500 kb on + strand, within EX31_RS17820 at 119.500 kb on + strand, within EX31_RS17820 at 119.500 kb on + strand, within EX31_RS17820 at 119.500 kb on + strand, within EX31_RS17820 at 119.501 kb on - strand, within EX31_RS17820 at 119.501 kb on - strand, within EX31_RS17820 at 119.501 kb on - strand, within EX31_RS17820 at 119.501 kb on - strand, within EX31_RS17820 at 119.501 kb on - strand, within EX31_RS17820 at 119.535 kb on - strand, within EX31_RS17820 at 119.610 kb on - strand, within EX31_RS17820 at 119.665 kb on + strand, within EX31_RS17820 at 119.665 kb on + strand, within EX31_RS17820 at 119.666 kb on - strand, within EX31_RS17820 at 119.744 kb on + strand, within EX31_RS17820 at 119.745 kb on - strand, within EX31_RS17820 at 119.745 kb on - strand, within EX31_RS17820 at 119.816 kb on - strand at 119.836 kb on + strand at 119.837 kb on - strand at 119.837 kb on - strand at 119.837 kb on - strand at 119.837 kb on - strand at 119.847 kb on + strand at 119.930 kb on + strand at 119.944 kb on - strand at 120.035 kb on - strand at 120.035 kb on - strand at 120.035 kb on - strand at 120.035 kb on - strand at 120.178 kb on - strand, within EX31_RS17825 at 120.178 kb on - strand, within EX31_RS17825 at 120.178 kb on - strand, within EX31_RS17825
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 117,947 + -1.3 118,275 + EX31_RS17810 0.37 +1.3 118,278 + EX31_RS17810 0.37 +0.1 118,310 + EX31_RS17810 0.41 -0.6 118,310 + EX31_RS17810 0.41 +0.7 118,576 - EX31_RS17810 0.72 +0.2 118,600 + EX31_RS17810 0.75 -0.8 118,600 + EX31_RS17810 0.75 +0.4 118,601 - EX31_RS17810 0.75 +0.9 118,910 + EX31_RS17815 0.19 -0.4 118,912 + EX31_RS17815 0.20 +0.0 118,912 + EX31_RS17815 0.20 -1.1 118,913 - EX31_RS17815 0.20 +1.0 119,258 + EX31_RS17815 0.83 -0.7 119,258 + EX31_RS17815 0.83 +0.9 119,259 - EX31_RS17815 0.83 -0.6 119,259 - EX31_RS17815 0.83 -0.1 119,259 - EX31_RS17815 0.83 -0.3 119,259 - EX31_RS17815 0.83 -1.0 119,259 - EX31_RS17815 0.83 -1.4 119,259 - EX31_RS17815 0.83 -2.5 119,259 - EX31_RS17815 0.83 +1.2 119,361 - +0.5 119,500 + EX31_RS17820 0.25 +0.4 119,500 + EX31_RS17820 0.25 -0.7 119,500 + EX31_RS17820 0.25 -0.1 119,500 + EX31_RS17820 0.25 -1.0 119,500 + EX31_RS17820 0.25 -0.4 119,500 + EX31_RS17820 0.25 +0.5 119,501 - EX31_RS17820 0.26 +0.0 119,501 - EX31_RS17820 0.26 +0.6 119,501 - EX31_RS17820 0.26 -1.0 119,501 - EX31_RS17820 0.26 +0.3 119,501 - EX31_RS17820 0.26 -0.3 119,535 - EX31_RS17820 0.33 +0.0 119,610 - EX31_RS17820 0.48 -0.4 119,665 + EX31_RS17820 0.60 +0.2 119,665 + EX31_RS17820 0.60 +0.2 119,666 - EX31_RS17820 0.60 +0.6 119,744 + EX31_RS17820 0.77 +0.9 119,745 - EX31_RS17820 0.77 -1.8 119,745 - EX31_RS17820 0.77 +0.1 119,816 - -0.3 119,836 + +1.4 119,837 - -1.0 119,837 - +0.3 119,837 - +0.2 119,837 - -1.1 119,847 + +0.8 119,930 + -0.3 119,944 - +1.0 120,035 - -0.9 120,035 - -0.1 120,035 - +1.7 120,035 - -1.7 120,178 - EX31_RS17825 0.22 +0.1 120,178 - EX31_RS17825 0.22 -1.1 120,178 - EX31_RS17825 0.22 +2.0
Or see this region's nucleotide sequence