Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16265 and EX31_RS16270 are separated by 99 nucleotides EX31_RS16270 and EX31_RS16275 are separated by 57 nucleotides EX31_RS16275 and EX31_RS16280 are separated by 130 nucleotides
EX31_RS16265: EX31_RS16265 - hypothetical protein, at 4,024 to 4,389
_RS16265
EX31_RS16270: EX31_RS16270 - DUF945 domain-containing protein, at 4,489 to 5,313
_RS16270
EX31_RS16275: EX31_RS16275 - hypothetical protein, at 5,371 to 5,661
_RS16275
EX31_RS16280: EX31_RS16280 - lytic transglycosylase domain-containing protein, at 5,792 to 6,331
_RS16280
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.387 kb on + strand at 4.387 kb on + strand at 4.387 kb on + strand at 4.393 kb on + strand at 4.393 kb on + strand at 4.394 kb on - strand at 4.394 kb on - strand at 4.420 kb on + strand at 4.458 kb on + strand at 4.458 kb on + strand at 4.458 kb on + strand at 4.459 kb on - strand at 4.459 kb on - strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.489 kb on - strand at 4.496 kb on + strand at 4.496 kb on + strand at 4.618 kb on + strand, within EX31_RS16270 at 4.618 kb on + strand, within EX31_RS16270 at 4.619 kb on - strand, within EX31_RS16270 at 4.619 kb on - strand, within EX31_RS16270 at 4.620 kb on + strand, within EX31_RS16270 at 4.620 kb on + strand, within EX31_RS16270 at 4.620 kb on + strand, within EX31_RS16270 at 4.621 kb on - strand, within EX31_RS16270 at 4.621 kb on - strand, within EX31_RS16270 at 4.621 kb on - strand, within EX31_RS16270 at 4.621 kb on - strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.624 kb on + strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.625 kb on - strand, within EX31_RS16270 at 4.626 kb on + strand, within EX31_RS16270 at 4.626 kb on + strand, within EX31_RS16270 at 4.626 kb on + strand, within EX31_RS16270 at 4.626 kb on + strand, within EX31_RS16270 at 4.626 kb on + strand, within EX31_RS16270 at 4.627 kb on - strand, within EX31_RS16270 at 4.627 kb on - strand, within EX31_RS16270 at 4.797 kb on + strand, within EX31_RS16270 at 4.797 kb on + strand, within EX31_RS16270 at 4.797 kb on + strand, within EX31_RS16270 at 4.798 kb on - strand, within EX31_RS16270 at 4.798 kb on - strand, within EX31_RS16270 at 4.822 kb on + strand, within EX31_RS16270 at 4.823 kb on - strand, within EX31_RS16270 at 4.945 kb on + strand, within EX31_RS16270 at 4.946 kb on - strand, within EX31_RS16270 at 4.946 kb on - strand, within EX31_RS16270 at 4.946 kb on - strand, within EX31_RS16270 at 4.946 kb on - strand, within EX31_RS16270 at 4.989 kb on + strand, within EX31_RS16270 at 4.989 kb on + strand, within EX31_RS16270 at 4.990 kb on - strand, within EX31_RS16270 at 4.990 kb on - strand, within EX31_RS16270 at 4.990 kb on - strand, within EX31_RS16270 at 5.053 kb on + strand, within EX31_RS16270 at 5.053 kb on + strand, within EX31_RS16270 at 5.053 kb on + strand, within EX31_RS16270 at 5.054 kb on - strand, within EX31_RS16270 at 5.054 kb on - strand, within EX31_RS16270 at 5.054 kb on - strand, within EX31_RS16270 at 5.054 kb on - strand, within EX31_RS16270 at 5.054 kb on - strand, within EX31_RS16270 at 5.102 kb on + strand, within EX31_RS16270 at 5.103 kb on - strand, within EX31_RS16270 at 5.103 kb on - strand, within EX31_RS16270 at 5.103 kb on - strand, within EX31_RS16270 at 5.103 kb on - strand, within EX31_RS16270 at 5.103 kb on - strand, within EX31_RS16270 at 5.106 kb on - strand, within EX31_RS16270 at 5.173 kb on - strand, within EX31_RS16270 at 5.261 kb on + strand at 5.262 kb on - strand at 5.325 kb on + strand at 5.358 kb on + strand at 5.358 kb on + strand at 5.358 kb on + strand at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.409 kb on + strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.410 kb on - strand, within EX31_RS16275 at 5.411 kb on + strand, within EX31_RS16275 at 5.411 kb on + strand, within EX31_RS16275 at 5.411 kb on + strand, within EX31_RS16275 at 5.412 kb on - strand, within EX31_RS16275 at 5.412 kb on - strand, within EX31_RS16275 at 5.412 kb on - strand, within EX31_RS16275 at 5.443 kb on + strand, within EX31_RS16275 at 5.443 kb on + strand, within EX31_RS16275 at 5.444 kb on - strand, within EX31_RS16275 at 5.495 kb on + strand, within EX31_RS16275 at 5.496 kb on - strand, within EX31_RS16275 at 5.510 kb on - strand, within EX31_RS16275 at 5.577 kb on + strand, within EX31_RS16275 at 5.577 kb on + strand, within EX31_RS16275 at 5.732 kb on + strand at 5.733 kb on - strand at 5.767 kb on - strand at 5.767 kb on - strand at 5.767 kb on - strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.769 kb on - strand at 5.785 kb on + strand at 5.786 kb on - strand at 5.794 kb on - strand at 5.815 kb on + strand at 5.816 kb on - strand at 5.816 kb on - strand at 5.910 kb on + strand, within EX31_RS16280 at 5.911 kb on - strand, within EX31_RS16280 at 5.914 kb on + strand, within EX31_RS16280 at 5.914 kb on + strand, within EX31_RS16280 at 5.979 kb on + strand, within EX31_RS16280 at 5.980 kb on - strand, within EX31_RS16280 at 5.980 kb on - strand, within EX31_RS16280 at 5.980 kb on - strand, within EX31_RS16280 at 6.048 kb on + strand, within EX31_RS16280 at 6.048 kb on + strand, within EX31_RS16280 at 6.052 kb on + strand, within EX31_RS16280 at 6.052 kb on + strand, within EX31_RS16280 at 6.053 kb on - strand, within EX31_RS16280 at 6.091 kb on + strand, within EX31_RS16280 at 6.127 kb on + strand, within EX31_RS16280 at 6.158 kb on - strand, within EX31_RS16280 at 6.158 kb on - strand, within EX31_RS16280 at 6.243 kb on + strand, within EX31_RS16280 at 6.263 kb on - strand, within EX31_RS16280 at 6.338 kb on - strand at 6.339 kb on + strand at 6.415 kb on + strand at 6.416 kb on - strand at 6.416 kb on - strand at 6.423 kb on + strand at 6.498 kb on + strand at 6.499 kb on - strand at 6.575 kb on - strand at 6.575 kb on - strand at 6.605 kb on + strand at 6.606 kb on - strand at 6.617 kb on + strand at 6.618 kb on - strand at 6.618 kb on - strand at 6.640 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 4,387 + +2.1 4,387 + -0.7 4,387 + +2.3 4,393 + +1.3 4,393 + +0.3 4,394 - +0.8 4,394 - +0.3 4,420 + +0.7 4,458 + +0.9 4,458 + -0.9 4,458 + +1.9 4,459 - +0.4 4,459 - +0.2 4,488 + +1.3 4,488 + +0.4 4,489 - -0.2 4,496 + +0.3 4,496 + +0.8 4,618 + EX31_RS16270 0.16 -0.7 4,618 + EX31_RS16270 0.16 +1.1 4,619 - EX31_RS16270 0.16 +0.2 4,619 - EX31_RS16270 0.16 +0.4 4,620 + EX31_RS16270 0.16 -1.5 4,620 + EX31_RS16270 0.16 +0.6 4,620 + EX31_RS16270 0.16 +0.6 4,621 - EX31_RS16270 0.16 -0.4 4,621 - EX31_RS16270 0.16 -1.4 4,621 - EX31_RS16270 0.16 +1.1 4,621 - EX31_RS16270 0.16 -1.4 4,624 + EX31_RS16270 0.16 -0.7 4,624 + EX31_RS16270 0.16 -0.1 4,624 + EX31_RS16270 0.16 -1.2 4,624 + EX31_RS16270 0.16 +1.7 4,624 + EX31_RS16270 0.16 +1.0 4,624 + EX31_RS16270 0.16 +1.0 4,624 + EX31_RS16270 0.16 -0.7 4,624 + EX31_RS16270 0.16 -1.1 4,624 + EX31_RS16270 0.16 +1.3 4,624 + EX31_RS16270 0.16 +0.9 4,624 + EX31_RS16270 0.16 +0.3 4,624 + EX31_RS16270 0.16 +1.3 4,624 + EX31_RS16270 0.16 +1.9 4,624 + EX31_RS16270 0.16 -0.5 4,625 - EX31_RS16270 0.16 +0.8 4,625 - EX31_RS16270 0.16 -0.2 4,625 - EX31_RS16270 0.16 +1.0 4,625 - EX31_RS16270 0.16 +0.2 4,625 - EX31_RS16270 0.16 -0.0 4,625 - EX31_RS16270 0.16 +0.3 4,625 - EX31_RS16270 0.16 +1.8 4,625 - EX31_RS16270 0.16 -0.2 4,625 - EX31_RS16270 0.16 +0.2 4,625 - EX31_RS16270 0.16 +0.8 4,625 - EX31_RS16270 0.16 -1.0 4,625 - EX31_RS16270 0.16 -1.5 4,625 - EX31_RS16270 0.16 -0.8 4,625 - EX31_RS16270 0.16 +0.1 4,625 - EX31_RS16270 0.16 -1.0 4,625 - EX31_RS16270 0.16 -0.4 4,625 - EX31_RS16270 0.16 +2.0 4,625 - EX31_RS16270 0.16 -0.7 4,625 - EX31_RS16270 0.16 -0.5 4,625 - EX31_RS16270 0.16 -0.4 4,625 - EX31_RS16270 0.16 -1.5 4,625 - EX31_RS16270 0.16 -0.4 4,625 - EX31_RS16270 0.16 -1.0 4,626 + EX31_RS16270 0.17 -1.4 4,626 + EX31_RS16270 0.17 -0.7 4,626 + EX31_RS16270 0.17 -0.7 4,626 + EX31_RS16270 0.17 +1.5 4,626 + EX31_RS16270 0.17 -0.2 4,627 - EX31_RS16270 0.17 +0.0 4,627 - EX31_RS16270 0.17 +1.9 4,797 + EX31_RS16270 0.37 -0.8 4,797 + EX31_RS16270 0.37 -0.8 4,797 + EX31_RS16270 0.37 -0.1 4,798 - EX31_RS16270 0.37 +1.5 4,798 - EX31_RS16270 0.37 +0.8 4,822 + EX31_RS16270 0.40 +0.9 4,823 - EX31_RS16270 0.40 +0.7 4,945 + EX31_RS16270 0.55 -1.2 4,946 - EX31_RS16270 0.55 -0.5 4,946 - EX31_RS16270 0.55 +1.8 4,946 - EX31_RS16270 0.55 +0.8 4,946 - EX31_RS16270 0.55 -0.5 4,989 + EX31_RS16270 0.61 +2.3 4,989 + EX31_RS16270 0.61 -0.7 4,990 - EX31_RS16270 0.61 -0.6 4,990 - EX31_RS16270 0.61 -0.2 4,990 - EX31_RS16270 0.61 +0.3 5,053 + EX31_RS16270 0.68 +1.0 5,053 + EX31_RS16270 0.68 -0.1 5,053 + EX31_RS16270 0.68 +0.5 5,054 - EX31_RS16270 0.68 +2.3 5,054 - EX31_RS16270 0.68 -0.1 5,054 - EX31_RS16270 0.68 -0.5 5,054 - EX31_RS16270 0.68 +1.0 5,054 - EX31_RS16270 0.68 +1.3 5,102 + EX31_RS16270 0.74 +0.7 5,103 - EX31_RS16270 0.74 -1.1 5,103 - EX31_RS16270 0.74 -2.2 5,103 - EX31_RS16270 0.74 +2.3 5,103 - EX31_RS16270 0.74 -0.5 5,103 - EX31_RS16270 0.74 +0.9 5,106 - EX31_RS16270 0.75 +0.8 5,173 - EX31_RS16270 0.83 +1.5 5,261 + -0.1 5,262 - -0.4 5,325 + +0.1 5,358 + +0.0 5,358 + -0.2 5,358 + +1.5 5,409 + EX31_RS16275 0.13 +0.5 5,409 + EX31_RS16275 0.13 +0.8 5,409 + EX31_RS16275 0.13 +0.0 5,409 + EX31_RS16275 0.13 +0.4 5,409 + EX31_RS16275 0.13 +0.3 5,409 + EX31_RS16275 0.13 -0.5 5,409 + EX31_RS16275 0.13 +1.3 5,409 + EX31_RS16275 0.13 +1.1 5,409 + EX31_RS16275 0.13 +0.6 5,410 - EX31_RS16275 0.13 -0.1 5,410 - EX31_RS16275 0.13 +0.1 5,410 - EX31_RS16275 0.13 +0.6 5,410 - EX31_RS16275 0.13 +0.5 5,410 - EX31_RS16275 0.13 +0.7 5,410 - EX31_RS16275 0.13 -0.4 5,411 + EX31_RS16275 0.14 +0.0 5,411 + EX31_RS16275 0.14 -1.7 5,411 + EX31_RS16275 0.14 -1.1 5,412 - EX31_RS16275 0.14 -0.7 5,412 - EX31_RS16275 0.14 +0.3 5,412 - EX31_RS16275 0.14 -0.7 5,443 + EX31_RS16275 0.25 -0.6 5,443 + EX31_RS16275 0.25 -0.5 5,444 - EX31_RS16275 0.25 +1.8 5,495 + EX31_RS16275 0.43 -0.0 5,496 - EX31_RS16275 0.43 -0.5 5,510 - EX31_RS16275 0.48 +2.3 5,577 + EX31_RS16275 0.71 +1.3 5,577 + EX31_RS16275 0.71 -0.5 5,732 + +1.5 5,733 - +1.1 5,767 - +0.5 5,767 - -0.5 5,767 - +1.3 5,768 + +1.0 5,768 + +0.2 5,769 - +0.3 5,785 + +0.8 5,786 - -0.5 5,794 - -0.1 5,815 + +1.0 5,816 - +0.1 5,816 - +0.4 5,910 + EX31_RS16280 0.22 +0.7 5,911 - EX31_RS16280 0.22 -0.3 5,914 + EX31_RS16280 0.23 +1.0 5,914 + EX31_RS16280 0.23 +0.8 5,979 + EX31_RS16280 0.35 -0.2 5,980 - EX31_RS16280 0.35 +0.3 5,980 - EX31_RS16280 0.35 -0.1 5,980 - EX31_RS16280 0.35 -2.7 6,048 + EX31_RS16280 0.47 +1.6 6,048 + EX31_RS16280 0.47 +0.2 6,052 + EX31_RS16280 0.48 -0.3 6,052 + EX31_RS16280 0.48 -0.8 6,053 - EX31_RS16280 0.48 +1.1 6,091 + EX31_RS16280 0.55 -0.2 6,127 + EX31_RS16280 0.62 -0.2 6,158 - EX31_RS16280 0.68 +0.4 6,158 - EX31_RS16280 0.68 -0.5 6,243 + EX31_RS16280 0.84 +1.1 6,263 - EX31_RS16280 0.87 -1.2 6,338 - +0.7 6,339 + +1.0 6,415 + +0.3 6,416 - -2.2 6,416 - +0.2 6,423 + +0.6 6,498 + +1.3 6,499 - -1.0 6,575 - +0.5 6,575 - -0.8 6,605 + +0.0 6,606 - -0.2 6,617 + -0.3 6,618 - -0.2 6,618 - +1.8 6,640 + +0.9
Or see this region's nucleotide sequence