Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15530 and EX31_RS15535 overlap by 4 nucleotides EX31_RS15535 and EX31_RS15540 are separated by 133 nucleotides EX31_RS15540 and EX31_RS15545 are separated by 50 nucleotides EX31_RS15545 and EX31_RS15550 are separated by 4 nucleotides
EX31_RS15530: EX31_RS15530 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 3,091 to 3,531
_RS15530
EX31_RS15535: EX31_RS15535 - LamB/YcsF family protein, at 3,528 to 4,310
_RS15535
EX31_RS15540: EX31_RS15540 - SDR family oxidoreductase, at 4,444 to 5,193
_RS15540
EX31_RS15545: EX31_RS15545 - amino acid ABC transporter permease, at 5,244 to 5,939
_RS15545
EX31_RS15550: EX31_RS15550 - amino acid ABC transporter permease, at 5,944 to 6,693
_RS15550
Position (kb) 4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3.534 kb on + strand at 3.534 kb on + strand at 3.576 kb on + strand at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.786 kb on + strand, within EX31_RS15535 at 3.786 kb on + strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.796 kb on - strand, within EX31_RS15535 at 3.879 kb on - strand, within EX31_RS15535 at 4.232 kb on + strand at 4.232 kb on + strand at 4.232 kb on + strand at 4.232 kb on + strand at 4.233 kb on - strand at 4.233 kb on - strand at 4.309 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.323 kb on + strand at 4.323 kb on + strand at 4.323 kb on + strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.351 kb on + strand at 4.351 kb on + strand at 4.351 kb on + strand at 4.352 kb on - strand at 4.352 kb on - strand at 4.359 kb on - strand at 4.359 kb on - strand at 4.361 kb on - strand at 4.361 kb on - strand at 4.600 kb on - strand, within EX31_RS15540 at 4.600 kb on - strand, within EX31_RS15540 at 4.853 kb on - strand, within EX31_RS15540 at 4.853 kb on - strand, within EX31_RS15540 at 4.919 kb on + strand, within EX31_RS15540 at 5.205 kb on + strand at 5.205 kb on + strand at 5.206 kb on - strand at 5.354 kb on + strand, within EX31_RS15545 at 5.421 kb on + strand, within EX31_RS15545 at 5.421 kb on + strand, within EX31_RS15545 at 5.422 kb on - strand, within EX31_RS15545 at 5.422 kb on - strand, within EX31_RS15545 at 5.430 kb on + strand, within EX31_RS15545 at 5.431 kb on - strand, within EX31_RS15545 at 5.431 kb on - strand, within EX31_RS15545 at 5.454 kb on - strand, within EX31_RS15545 at 5.472 kb on + strand, within EX31_RS15545 at 5.662 kb on - strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.793 kb on + strand, within EX31_RS15545 at 5.794 kb on - strand, within EX31_RS15545 at 5.794 kb on - strand, within EX31_RS15545 at 5.808 kb on - strand, within EX31_RS15545 at 5.842 kb on - strand, within EX31_RS15545 at 5.863 kb on - strand, within EX31_RS15545 at 5.937 kb on + strand at 5.938 kb on - strand at 5.938 kb on - strand at 6.033 kb on + strand, within EX31_RS15550 at 6.034 kb on - strand, within EX31_RS15550 at 6.034 kb on - strand, within EX31_RS15550 at 6.081 kb on + strand, within EX31_RS15550 at 6.148 kb on + strand, within EX31_RS15550 at 6.193 kb on + strand, within EX31_RS15550
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 3,534 + +1.1 3,534 + -0.1 3,576 + +1.4 3,700 + EX31_RS15535 0.22 -0.2 3,700 + EX31_RS15535 0.22 +1.6 3,700 + EX31_RS15535 0.22 -0.8 3,700 + EX31_RS15535 0.22 +2.0 3,700 + EX31_RS15535 0.22 +0.5 3,700 + EX31_RS15535 0.22 +0.2 3,786 + EX31_RS15535 0.33 +0.2 3,786 + EX31_RS15535 0.33 +0.4 3,787 - EX31_RS15535 0.33 +0.2 3,787 - EX31_RS15535 0.33 -1.2 3,787 - EX31_RS15535 0.33 +0.4 3,796 - EX31_RS15535 0.34 +1.6 3,879 - EX31_RS15535 0.45 -0.5 4,232 + +0.4 4,232 + -0.3 4,232 + -0.6 4,232 + -0.2 4,233 - +0.4 4,233 - -0.8 4,309 - -0.3 4,322 - +0.3 4,322 - +0.8 4,322 - -0.3 4,323 + +0.0 4,323 + -2.4 4,323 + +1.0 4,324 - +0.3 4,324 - -1.9 4,324 - -0.4 4,324 - -1.2 4,324 - -0.8 4,351 + +0.7 4,351 + +0.1 4,351 + +0.1 4,352 - +1.3 4,352 - -0.6 4,359 - +0.1 4,359 - -0.6 4,361 - +0.0 4,361 - +0.9 4,600 - EX31_RS15540 0.21 -1.6 4,600 - EX31_RS15540 0.21 +0.7 4,853 - EX31_RS15540 0.55 +0.2 4,853 - EX31_RS15540 0.55 -1.1 4,919 + EX31_RS15540 0.63 -0.8 5,205 + +0.2 5,205 + -0.7 5,206 - +0.4 5,354 + EX31_RS15545 0.16 +2.3 5,421 + EX31_RS15545 0.25 -0.8 5,421 + EX31_RS15545 0.25 -0.5 5,422 - EX31_RS15545 0.26 -1.1 5,422 - EX31_RS15545 0.26 +1.4 5,430 + EX31_RS15545 0.27 +0.3 5,431 - EX31_RS15545 0.27 +0.2 5,431 - EX31_RS15545 0.27 +0.2 5,454 - EX31_RS15545 0.30 +1.0 5,472 + EX31_RS15545 0.33 -0.8 5,662 - EX31_RS15545 0.60 +0.5 5,793 + EX31_RS15545 0.79 -0.7 5,793 + EX31_RS15545 0.79 +0.7 5,793 + EX31_RS15545 0.79 +1.9 5,793 + EX31_RS15545 0.79 -0.3 5,793 + EX31_RS15545 0.79 -1.2 5,793 + EX31_RS15545 0.79 -0.3 5,794 - EX31_RS15545 0.79 -0.4 5,794 - EX31_RS15545 0.79 +1.0 5,808 - EX31_RS15545 0.81 +0.0 5,842 - EX31_RS15545 0.86 +1.8 5,863 - EX31_RS15545 0.89 +0.8 5,937 + -1.4 5,938 - -1.2 5,938 - +1.8 6,033 + EX31_RS15550 0.12 +2.4 6,034 - EX31_RS15550 0.12 -1.4 6,034 - EX31_RS15550 0.12 +0.4 6,081 + EX31_RS15550 0.18 +0.0 6,148 + EX31_RS15550 0.27 -0.6 6,193 + EX31_RS15550 0.33 +2.0
Or see this region's nucleotide sequence