Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13735 and EX31_RS13740 are separated by 173 nucleotides EX31_RS13740 and EX31_RS13745 are separated by 92 nucleotides EX31_RS13745 and EX31_RS13750 are separated by 103 nucleotides
EX31_RS13735: EX31_RS13735 - 4-hydroxy-tetrahydrodipicolinate synthase, at 7,080 to 7,946
_RS13735
EX31_RS13740: EX31_RS13740 - glutathione transferase GstA, at 8,120 to 8,725
_RS13740
EX31_RS13745: EX31_RS13745 - pyridoxal kinase PdxY, at 8,818 to 9,678
_RS13745
EX31_RS13750: EX31_RS13750 - tyrosine--tRNA ligase, at 9,782 to 11,056
_RS13750
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 7.880 kb on + strand at 7.881 kb on - strand at 7.952 kb on + strand at 7.952 kb on + strand at 7.952 kb on + strand at 7.953 kb on - strand at 7.996 kb on - strand at 8.025 kb on + strand at 8.030 kb on + strand at 8.030 kb on + strand at 8.031 kb on - strand at 8.031 kb on - strand at 8.043 kb on + strand at 8.045 kb on + strand at 8.045 kb on + strand at 8.046 kb on - strand at 8.048 kb on - strand at 8.048 kb on - strand at 8.129 kb on + strand at 8.129 kb on + strand at 8.129 kb on + strand at 8.130 kb on - strand at 8.130 kb on - strand at 8.132 kb on + strand at 8.132 kb on + strand at 8.132 kb on + strand at 8.132 kb on + strand at 8.132 kb on + strand at 8.164 kb on + strand at 8.164 kb on + strand at 8.164 kb on + strand at 8.417 kb on + strand, within EX31_RS13740 at 8.418 kb on - strand, within EX31_RS13740 at 8.418 kb on - strand, within EX31_RS13740 at 8.588 kb on + strand, within EX31_RS13740 at 8.589 kb on - strand, within EX31_RS13740 at 8.589 kb on - strand, within EX31_RS13740 at 8.660 kb on + strand, within EX31_RS13740 at 8.724 kb on - strand at 8.768 kb on + strand at 8.927 kb on + strand, within EX31_RS13745 at 8.927 kb on + strand, within EX31_RS13745 at 8.927 kb on + strand, within EX31_RS13745 at 8.928 kb on - strand, within EX31_RS13745 at 9.315 kb on + strand, within EX31_RS13745 at 9.434 kb on + strand, within EX31_RS13745 at 9.570 kb on + strand, within EX31_RS13745 at 9.571 kb on - strand, within EX31_RS13745 at 9.571 kb on - strand, within EX31_RS13745 at 9.571 kb on - strand, within EX31_RS13745 at 9.727 kb on + strand at 9.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 7,880 + +0.4 7,881 - +0.6 7,952 + -0.9 7,952 + -1.0 7,952 + -0.6 7,953 - +1.6 7,996 - -2.1 8,025 + +0.0 8,030 + -0.1 8,030 + +0.5 8,031 - +1.2 8,031 - -1.3 8,043 + +0.8 8,045 + -2.3 8,045 + +0.8 8,046 - +0.5 8,048 - +0.1 8,048 - -0.9 8,129 + -0.1 8,129 + +0.9 8,129 + +0.4 8,130 - -2.0 8,130 - -0.8 8,132 + +1.1 8,132 + +0.8 8,132 + +2.0 8,132 + +1.0 8,132 + +0.3 8,164 + -0.2 8,164 + +1.3 8,164 + +0.9 8,417 + EX31_RS13740 0.49 -0.8 8,418 - EX31_RS13740 0.49 +0.3 8,418 - EX31_RS13740 0.49 +1.0 8,588 + EX31_RS13740 0.77 -0.6 8,589 - EX31_RS13740 0.77 +1.3 8,589 - EX31_RS13740 0.77 +0.3 8,660 + EX31_RS13740 0.89 +0.3 8,724 - -0.2 8,768 + +0.0 8,927 + EX31_RS13745 0.13 +0.4 8,927 + EX31_RS13745 0.13 -0.3 8,927 + EX31_RS13745 0.13 +2.3 8,928 - EX31_RS13745 0.13 +1.3 9,315 + EX31_RS13745 0.58 +2.8 9,434 + EX31_RS13745 0.72 +0.5 9,570 + EX31_RS13745 0.87 +0.7 9,571 - EX31_RS13745 0.87 +1.4 9,571 - EX31_RS13745 0.87 +0.0 9,571 - EX31_RS13745 0.87 +1.1 9,727 + +2.0 9,776 + -1.4
Or see this region's nucleotide sequence