Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12820 and EX31_RS12825 are separated by 160 nucleotides EX31_RS12825 and EX31_RS12830 are separated by 168 nucleotides EX31_RS12830 and EX31_RS12835 are separated by 36 nucleotides
EX31_RS12820: EX31_RS12820 - DUF3459 domain-containing protein, at 237,254 to 239,056
_RS12820
EX31_RS12825: EX31_RS12825 - ornithine carbamoyltransferase, at 239,217 to 240,224
_RS12825
EX31_RS12830: EX31_RS12830 - ribonuclease E inhibitor RraB, at 240,393 to 240,818
_RS12830
EX31_RS12835: EX31_RS12835 - 6-phospho-beta-glucosidase, at 240,855 to 242,288
_RS12835
Position (kb) 239
240
241 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 238.282 kb on + strand, within EX31_RS12820 at 238.291 kb on + strand, within EX31_RS12820 at 238.292 kb on - strand, within EX31_RS12820 at 238.292 kb on - strand, within EX31_RS12820 at 238.292 kb on - strand, within EX31_RS12820 at 238.339 kb on + strand, within EX31_RS12820 at 238.339 kb on + strand, within EX31_RS12820 at 238.340 kb on - strand, within EX31_RS12820 at 238.341 kb on + strand, within EX31_RS12820 at 238.341 kb on + strand, within EX31_RS12820 at 238.341 kb on + strand, within EX31_RS12820 at 238.341 kb on + strand, within EX31_RS12820 at 238.342 kb on - strand, within EX31_RS12820 at 238.342 kb on - strand, within EX31_RS12820 at 238.386 kb on + strand, within EX31_RS12820 at 238.387 kb on - strand, within EX31_RS12820 at 238.438 kb on + strand, within EX31_RS12820 at 238.439 kb on - strand, within EX31_RS12820 at 238.476 kb on + strand, within EX31_RS12820 at 238.476 kb on + strand, within EX31_RS12820 at 238.476 kb on + strand, within EX31_RS12820 at 238.476 kb on + strand, within EX31_RS12820 at 238.477 kb on - strand, within EX31_RS12820 at 238.548 kb on + strand, within EX31_RS12820 at 238.574 kb on - strand, within EX31_RS12820 at 238.578 kb on + strand, within EX31_RS12820 at 238.578 kb on + strand, within EX31_RS12820 at 238.587 kb on + strand, within EX31_RS12820 at 238.588 kb on - strand, within EX31_RS12820 at 238.591 kb on - strand, within EX31_RS12820 at 238.608 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.639 kb on + strand, within EX31_RS12820 at 238.640 kb on - strand, within EX31_RS12820 at 238.640 kb on - strand, within EX31_RS12820 at 238.693 kb on + strand, within EX31_RS12820 at 238.693 kb on + strand, within EX31_RS12820 at 238.765 kb on - strand, within EX31_RS12820 at 238.765 kb on - strand, within EX31_RS12820 at 238.770 kb on + strand, within EX31_RS12820 at 238.770 kb on + strand, within EX31_RS12820 at 238.771 kb on - strand, within EX31_RS12820 at 238.771 kb on - strand, within EX31_RS12820 at 238.771 kb on - strand, within EX31_RS12820 at 238.783 kb on + strand, within EX31_RS12820 at 238.783 kb on + strand, within EX31_RS12820 at 238.799 kb on - strand, within EX31_RS12820 at 238.812 kb on + strand, within EX31_RS12820 at 238.812 kb on + strand, within EX31_RS12820 at 238.812 kb on + strand, within EX31_RS12820 at 238.813 kb on - strand, within EX31_RS12820 at 238.813 kb on - strand, within EX31_RS12820 at 238.813 kb on - strand, within EX31_RS12820 at 238.867 kb on + strand, within EX31_RS12820 at 238.870 kb on + strand, within EX31_RS12820 at 238.871 kb on - strand, within EX31_RS12820 at 238.878 kb on + strand at 238.878 kb on + strand at 238.878 kb on + strand at 238.878 kb on + strand at 238.882 kb on + strand at 238.882 kb on + strand at 238.882 kb on + strand at 238.882 kb on + strand at 238.883 kb on - strand at 238.883 kb on - strand at 238.883 kb on - strand at 238.883 kb on - strand at 238.883 kb on - strand at 238.883 kb on - strand at 238.890 kb on + strand at 238.890 kb on + strand at 238.894 kb on + strand at 238.895 kb on - strand at 238.895 kb on - strand at 238.895 kb on - strand at 238.895 kb on - strand at 238.899 kb on + strand at 238.998 kb on + strand at 238.999 kb on - strand at 239.010 kb on - strand at 239.010 kb on - strand at 239.010 kb on - strand at 239.034 kb on + strand at 239.035 kb on - strand at 239.035 kb on - strand at 239.057 kb on - strand at 239.150 kb on - strand at 239.191 kb on + strand at 239.191 kb on + strand at 239.192 kb on - strand at 239.258 kb on + strand at 239.258 kb on + strand at 239.258 kb on + strand at 239.286 kb on + strand at 239.287 kb on - strand at 239.287 kb on - strand at 239.289 kb on + strand at 239.289 kb on + strand at 239.347 kb on + strand, within EX31_RS12825 at 239.347 kb on + strand, within EX31_RS12825 at 239.473 kb on - strand, within EX31_RS12825 at 239.480 kb on + strand, within EX31_RS12825 at 239.481 kb on - strand, within EX31_RS12825 at 239.537 kb on - strand, within EX31_RS12825 at 239.537 kb on - strand, within EX31_RS12825 at 239.537 kb on - strand, within EX31_RS12825 at 239.570 kb on + strand, within EX31_RS12825 at 239.721 kb on - strand, within EX31_RS12825 at 239.721 kb on - strand, within EX31_RS12825 at 239.721 kb on - strand, within EX31_RS12825 at 239.743 kb on + strand, within EX31_RS12825 at 239.744 kb on - strand, within EX31_RS12825 at 239.889 kb on - strand, within EX31_RS12825 at 239.909 kb on - strand, within EX31_RS12825 at 239.915 kb on + strand, within EX31_RS12825 at 239.916 kb on - strand, within EX31_RS12825 at 239.923 kb on + strand, within EX31_RS12825 at 239.923 kb on + strand, within EX31_RS12825 at 239.924 kb on - strand, within EX31_RS12825 at 239.998 kb on + strand, within EX31_RS12825 at 239.998 kb on + strand, within EX31_RS12825 at 239.998 kb on + strand, within EX31_RS12825 at 239.999 kb on - strand, within EX31_RS12825 at 240.022 kb on + strand, within EX31_RS12825 at 240.023 kb on - strand, within EX31_RS12825 at 240.023 kb on - strand, within EX31_RS12825 at 240.135 kb on + strand at 240.210 kb on - strand at 240.244 kb on + strand at 240.245 kb on - strand at 240.309 kb on - strand at 240.324 kb on + strand at 240.326 kb on + strand at 240.326 kb on + strand at 240.326 kb on + strand at 240.327 kb on - strand at 240.327 kb on - strand at 240.327 kb on - strand at 240.357 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.362 kb on + strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.363 kb on - strand at 240.393 kb on - strand at 240.440 kb on - strand, within EX31_RS12830 at 240.440 kb on - strand, within EX31_RS12830 at 240.486 kb on + strand, within EX31_RS12830 at 240.486 kb on + strand, within EX31_RS12830 at 240.487 kb on - strand, within EX31_RS12830 at 240.487 kb on - strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.505 kb on + strand, within EX31_RS12830 at 240.506 kb on - strand, within EX31_RS12830 at 240.506 kb on - strand, within EX31_RS12830 at 240.506 kb on - strand, within EX31_RS12830 at 240.506 kb on - strand, within EX31_RS12830 at 240.506 kb on - strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.714 kb on + strand, within EX31_RS12830 at 240.715 kb on - strand, within EX31_RS12830 at 240.715 kb on - strand, within EX31_RS12830 at 240.715 kb on - strand, within EX31_RS12830 at 240.715 kb on - strand, within EX31_RS12830 at 240.732 kb on + strand, within EX31_RS12830 at 240.732 kb on + strand, within EX31_RS12830 at 240.732 kb on + strand, within EX31_RS12830 at 240.732 kb on + strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.733 kb on - strand, within EX31_RS12830 at 240.816 kb on + strand at 240.856 kb on + strand at 240.856 kb on + strand at 240.856 kb on + strand at 240.857 kb on - strand at 240.857 kb on - strand at 240.857 kb on - strand at 240.873 kb on - strand at 240.873 kb on - strand at 240.982 kb on + strand at 240.996 kb on - strand at 241.028 kb on + strand, within EX31_RS12835 at 241.028 kb on + strand, within EX31_RS12835 at 241.028 kb on + strand, within EX31_RS12835 at 241.028 kb on + strand, within EX31_RS12835 at 241.028 kb on + strand, within EX31_RS12835 at 241.029 kb on - strand, within EX31_RS12835 at 241.029 kb on - strand, within EX31_RS12835 at 241.031 kb on - strand, within EX31_RS12835 at 241.031 kb on - strand, within EX31_RS12835 at 241.097 kb on - strand, within EX31_RS12835 at 241.097 kb on - strand, within EX31_RS12835 at 241.097 kb on - strand, within EX31_RS12835 at 241.097 kb on - strand, within EX31_RS12835 at 241.108 kb on + strand, within EX31_RS12835 at 241.172 kb on + strand, within EX31_RS12835 at 241.172 kb on + strand, within EX31_RS12835 at 241.173 kb on - strand, within EX31_RS12835 at 241.173 kb on - strand, within EX31_RS12835 at 241.176 kb on + strand, within EX31_RS12835 at 241.205 kb on + strand, within EX31_RS12835 at 241.206 kb on - strand, within EX31_RS12835 at 241.216 kb on + strand, within EX31_RS12835 at 241.216 kb on + strand, within EX31_RS12835 at 241.216 kb on + strand, within EX31_RS12835 at 241.217 kb on - strand, within EX31_RS12835 at 241.222 kb on - strand, within EX31_RS12835 at 241.222 kb on - strand, within EX31_RS12835 at 241.222 kb on - strand, within EX31_RS12835
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 238,282 + EX31_RS12820 0.57 +0.4 238,291 + EX31_RS12820 0.58 -0.8 238,292 - EX31_RS12820 0.58 +0.2 238,292 - EX31_RS12820 0.58 +0.7 238,292 - EX31_RS12820 0.58 +0.6 238,339 + EX31_RS12820 0.60 -0.5 238,339 + EX31_RS12820 0.60 -0.8 238,340 - EX31_RS12820 0.60 -2.0 238,341 + EX31_RS12820 0.60 +0.8 238,341 + EX31_RS12820 0.60 -1.2 238,341 + EX31_RS12820 0.60 -1.5 238,341 + EX31_RS12820 0.60 +0.5 238,342 - EX31_RS12820 0.60 -0.2 238,342 - EX31_RS12820 0.60 +1.3 238,386 + EX31_RS12820 0.63 +1.2 238,387 - EX31_RS12820 0.63 -0.3 238,438 + EX31_RS12820 0.66 +0.8 238,439 - EX31_RS12820 0.66 -0.3 238,476 + EX31_RS12820 0.68 -0.9 238,476 + EX31_RS12820 0.68 +1.5 238,476 + EX31_RS12820 0.68 -0.7 238,476 + EX31_RS12820 0.68 +1.4 238,477 - EX31_RS12820 0.68 -0.8 238,548 + EX31_RS12820 0.72 +0.1 238,574 - EX31_RS12820 0.73 -0.8 238,578 + EX31_RS12820 0.73 +0.3 238,578 + EX31_RS12820 0.73 +0.2 238,587 + EX31_RS12820 0.74 +0.5 238,588 - EX31_RS12820 0.74 -1.4 238,591 - EX31_RS12820 0.74 +1.6 238,608 + EX31_RS12820 0.75 -1.6 238,639 + EX31_RS12820 0.77 +0.6 238,639 + EX31_RS12820 0.77 +1.0 238,639 + EX31_RS12820 0.77 +0.1 238,639 + EX31_RS12820 0.77 +0.2 238,639 + EX31_RS12820 0.77 -0.4 238,639 + EX31_RS12820 0.77 +0.7 238,639 + EX31_RS12820 0.77 +0.3 238,640 - EX31_RS12820 0.77 -0.3 238,640 - EX31_RS12820 0.77 +0.4 238,693 + EX31_RS12820 0.80 -0.3 238,693 + EX31_RS12820 0.80 +0.2 238,765 - EX31_RS12820 0.84 +0.7 238,765 - EX31_RS12820 0.84 -1.3 238,770 + EX31_RS12820 0.84 -0.4 238,770 + EX31_RS12820 0.84 +0.5 238,771 - EX31_RS12820 0.84 +0.2 238,771 - EX31_RS12820 0.84 +0.3 238,771 - EX31_RS12820 0.84 -0.1 238,783 + EX31_RS12820 0.85 -0.2 238,783 + EX31_RS12820 0.85 -1.0 238,799 - EX31_RS12820 0.86 -1.5 238,812 + EX31_RS12820 0.86 -0.2 238,812 + EX31_RS12820 0.86 -0.1 238,812 + EX31_RS12820 0.86 +0.2 238,813 - EX31_RS12820 0.86 -0.7 238,813 - EX31_RS12820 0.86 -0.9 238,813 - EX31_RS12820 0.86 +0.2 238,867 + EX31_RS12820 0.89 +1.7 238,870 + EX31_RS12820 0.90 +4.1 238,871 - EX31_RS12820 0.90 -0.4 238,878 + -0.4 238,878 + -1.0 238,878 + +0.3 238,878 + +1.8 238,882 + -0.7 238,882 + -0.3 238,882 + -0.4 238,882 + -0.3 238,883 - +0.8 238,883 - -0.3 238,883 - +1.0 238,883 - +0.5 238,883 - -0.1 238,883 - -1.3 238,890 + -0.3 238,890 + -0.4 238,894 + +0.0 238,895 - +0.2 238,895 - -0.4 238,895 - -1.6 238,895 - -1.4 238,899 + +0.5 238,998 + +0.9 238,999 - -0.1 239,010 - +0.8 239,010 - -0.6 239,010 - +1.0 239,034 + -0.4 239,035 - +1.1 239,035 - -0.2 239,057 - +0.7 239,150 - -0.1 239,191 + -0.9 239,191 + -0.7 239,192 - +0.7 239,258 + -1.3 239,258 + -2.3 239,258 + -3.8 239,286 + -0.2 239,287 - -2.5 239,287 - -4.8 239,289 + -1.3 239,289 + -2.8 239,347 + EX31_RS12825 0.13 -1.6 239,347 + EX31_RS12825 0.13 -1.6 239,473 - EX31_RS12825 0.25 -1.9 239,480 + EX31_RS12825 0.26 -1.0 239,481 - EX31_RS12825 0.26 -1.7 239,537 - EX31_RS12825 0.32 -2.8 239,537 - EX31_RS12825 0.32 -3.5 239,537 - EX31_RS12825 0.32 -2.7 239,570 + EX31_RS12825 0.35 -2.5 239,721 - EX31_RS12825 0.50 -2.3 239,721 - EX31_RS12825 0.50 -1.4 239,721 - EX31_RS12825 0.50 -3.2 239,743 + EX31_RS12825 0.52 -2.4 239,744 - EX31_RS12825 0.52 -1.6 239,889 - EX31_RS12825 0.67 -1.8 239,909 - EX31_RS12825 0.69 -0.8 239,915 + EX31_RS12825 0.69 -2.4 239,916 - EX31_RS12825 0.69 -3.2 239,923 + EX31_RS12825 0.70 -2.0 239,923 + EX31_RS12825 0.70 +0.2 239,924 - EX31_RS12825 0.70 -2.5 239,998 + EX31_RS12825 0.77 -2.0 239,998 + EX31_RS12825 0.77 -1.2 239,998 + EX31_RS12825 0.77 -3.9 239,999 - EX31_RS12825 0.78 -2.8 240,022 + EX31_RS12825 0.80 -2.5 240,023 - EX31_RS12825 0.80 -3.3 240,023 - EX31_RS12825 0.80 -1.0 240,135 + -3.0 240,210 - -1.6 240,244 + +0.9 240,245 - +0.5 240,309 - -1.3 240,324 + +0.6 240,326 + +0.5 240,326 + +0.1 240,326 + -0.8 240,327 - +0.4 240,327 - +0.1 240,327 - +0.8 240,357 + +1.7 240,362 + +0.2 240,362 + +0.1 240,362 + +0.1 240,362 + +0.6 240,362 + +0.9 240,362 + +0.1 240,362 + +0.8 240,362 + +0.3 240,363 - +0.5 240,363 - -1.2 240,363 - +0.7 240,363 - -0.3 240,363 - -0.1 240,363 - +0.5 240,363 - +0.1 240,393 - +1.5 240,440 - EX31_RS12830 0.11 -0.7 240,440 - EX31_RS12830 0.11 -1.3 240,486 + EX31_RS12830 0.22 +0.9 240,486 + EX31_RS12830 0.22 +0.7 240,487 - EX31_RS12830 0.22 -0.6 240,487 - EX31_RS12830 0.22 +1.2 240,505 + EX31_RS12830 0.26 -0.5 240,505 + EX31_RS12830 0.26 +0.8 240,505 + EX31_RS12830 0.26 +0.4 240,505 + EX31_RS12830 0.26 -0.5 240,505 + EX31_RS12830 0.26 -0.1 240,505 + EX31_RS12830 0.26 +0.1 240,506 - EX31_RS12830 0.27 -1.4 240,506 - EX31_RS12830 0.27 +0.8 240,506 - EX31_RS12830 0.27 +2.1 240,506 - EX31_RS12830 0.27 -1.0 240,506 - EX31_RS12830 0.27 +1.5 240,714 + EX31_RS12830 0.75 +0.9 240,714 + EX31_RS12830 0.75 -0.1 240,714 + EX31_RS12830 0.75 +0.3 240,714 + EX31_RS12830 0.75 +0.2 240,714 + EX31_RS12830 0.75 +1.2 240,714 + EX31_RS12830 0.75 -0.9 240,715 - EX31_RS12830 0.76 +0.5 240,715 - EX31_RS12830 0.76 +0.2 240,715 - EX31_RS12830 0.76 +1.1 240,715 - EX31_RS12830 0.76 -1.4 240,732 + EX31_RS12830 0.80 +0.6 240,732 + EX31_RS12830 0.80 -0.6 240,732 + EX31_RS12830 0.80 -2.0 240,732 + EX31_RS12830 0.80 +1.0 240,733 - EX31_RS12830 0.80 +0.6 240,733 - EX31_RS12830 0.80 -1.2 240,733 - EX31_RS12830 0.80 +0.7 240,733 - EX31_RS12830 0.80 -0.8 240,733 - EX31_RS12830 0.80 +1.1 240,733 - EX31_RS12830 0.80 -0.2 240,816 + +0.3 240,856 + +0.1 240,856 + +1.6 240,856 + -1.8 240,857 - -0.7 240,857 - -0.3 240,857 - +0.3 240,873 - +0.3 240,873 - -0.2 240,982 + +0.0 240,996 - +0.8 241,028 + EX31_RS12835 0.12 +1.2 241,028 + EX31_RS12835 0.12 +1.0 241,028 + EX31_RS12835 0.12 +1.3 241,028 + EX31_RS12835 0.12 +0.3 241,028 + EX31_RS12835 0.12 +0.8 241,029 - EX31_RS12835 0.12 +0.9 241,029 - EX31_RS12835 0.12 -0.2 241,031 - EX31_RS12835 0.12 +1.8 241,031 - EX31_RS12835 0.12 -1.0 241,097 - EX31_RS12835 0.17 +0.9 241,097 - EX31_RS12835 0.17 +0.2 241,097 - EX31_RS12835 0.17 +0.5 241,097 - EX31_RS12835 0.17 +1.3 241,108 + EX31_RS12835 0.18 +0.3 241,172 + EX31_RS12835 0.22 -0.7 241,172 + EX31_RS12835 0.22 +1.0 241,173 - EX31_RS12835 0.22 +0.4 241,173 - EX31_RS12835 0.22 +1.4 241,176 + EX31_RS12835 0.22 -0.2 241,205 + EX31_RS12835 0.24 -1.0 241,206 - EX31_RS12835 0.24 -0.2 241,216 + EX31_RS12835 0.25 -1.3 241,216 + EX31_RS12835 0.25 +0.5 241,216 + EX31_RS12835 0.25 +1.0 241,217 - EX31_RS12835 0.25 +0.5 241,222 - EX31_RS12835 0.26 -2.0 241,222 - EX31_RS12835 0.26 -0.9 241,222 - EX31_RS12835 0.26 -2.6
Or see this region's nucleotide sequence