Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11990 and EX31_RS11995 are separated by 326 nucleotides EX31_RS11995 and EX31_RS12000 are separated by 85 nucleotides EX31_RS12000 and EX31_RS12005 are separated by 100 nucleotides
EX31_RS11990: EX31_RS11990 - excinuclease ABC subunit UvrA, at 59,572 to 62,427
_RS11990
EX31_RS11995: EX31_RS11995 - single-stranded DNA-binding protein, at 62,754 to 63,296
_RS11995
EX31_RS12000: EX31_RS12000 - amidohydrolase family protein, at 63,382 to 64,800
_RS12000
EX31_RS12005: EX31_RS12005 - LysR family transcriptional regulator, at 64,901 to 65,755
_RS12005
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 62.422 kb on - strand at 62.452 kb on - strand at 62.452 kb on - strand at 62.452 kb on - strand at 62.452 kb on - strand at 62.524 kb on + strand at 62.524 kb on + strand at 62.524 kb on + strand at 62.525 kb on - strand at 62.525 kb on - strand at 62.525 kb on - strand at 62.525 kb on - strand at 62.525 kb on - strand at 62.535 kb on + strand at 62.535 kb on + strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.577 kb on + strand at 62.578 kb on - strand at 62.578 kb on - strand at 62.595 kb on + strand at 62.595 kb on + strand at 62.595 kb on + strand at 62.595 kb on + strand at 62.595 kb on + strand at 62.656 kb on - strand at 63.369 kb on + strand at 63.369 kb on + strand at 63.370 kb on - strand at 63.410 kb on - strand at 63.410 kb on - strand at 63.410 kb on - strand at 63.604 kb on - strand, within EX31_RS12000 at 63.604 kb on - strand, within EX31_RS12000 at 63.604 kb on - strand, within EX31_RS12000 at 63.620 kb on + strand, within EX31_RS12000 at 63.703 kb on + strand, within EX31_RS12000 at 63.703 kb on + strand, within EX31_RS12000 at 63.703 kb on + strand, within EX31_RS12000 at 63.704 kb on - strand, within EX31_RS12000 at 63.704 kb on - strand, within EX31_RS12000 at 63.704 kb on - strand, within EX31_RS12000 at 63.704 kb on - strand, within EX31_RS12000 at 63.704 kb on - strand, within EX31_RS12000 at 63.736 kb on - strand, within EX31_RS12000 at 63.736 kb on - strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.902 kb on + strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.903 kb on - strand, within EX31_RS12000 at 63.949 kb on - strand, within EX31_RS12000 at 63.949 kb on - strand, within EX31_RS12000 at 63.949 kb on - strand, within EX31_RS12000 at 63.949 kb on - strand, within EX31_RS12000 at 63.961 kb on - strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.046 kb on + strand, within EX31_RS12000 at 64.047 kb on - strand, within EX31_RS12000 at 64.047 kb on - strand, within EX31_RS12000 at 64.047 kb on - strand, within EX31_RS12000 at 64.292 kb on - strand, within EX31_RS12000 at 64.382 kb on + strand, within EX31_RS12000 at 64.382 kb on + strand, within EX31_RS12000 at 64.382 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.404 kb on + strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.405 kb on - strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.435 kb on + strand, within EX31_RS12000 at 64.436 kb on - strand, within EX31_RS12000 at 64.436 kb on - strand, within EX31_RS12000 at 64.436 kb on - strand, within EX31_RS12000 at 64.529 kb on + strand, within EX31_RS12000 at 64.529 kb on + strand, within EX31_RS12000 at 64.530 kb on - strand, within EX31_RS12000 at 64.530 kb on - strand, within EX31_RS12000 at 64.530 kb on - strand, within EX31_RS12000 at 64.607 kb on + strand, within EX31_RS12000 at 64.695 kb on + strand at 64.695 kb on + strand at 64.696 kb on - strand at 64.795 kb on - strand at 64.838 kb on + strand at 64.838 kb on + strand at 64.902 kb on + strand at 64.906 kb on + strand at 64.906 kb on + strand at 64.906 kb on + strand at 64.907 kb on - strand at 64.907 kb on - strand at 64.907 kb on - strand at 65.063 kb on + strand, within EX31_RS12005 at 65.064 kb on - strand, within EX31_RS12005 at 65.124 kb on + strand, within EX31_RS12005 at 65.124 kb on + strand, within EX31_RS12005 at 65.131 kb on + strand, within EX31_RS12005 at 65.140 kb on + strand, within EX31_RS12005 at 65.140 kb on + strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.182 kb on - strand, within EX31_RS12005 at 65.182 kb on - strand, within EX31_RS12005 at 65.390 kb on - strand, within EX31_RS12005 at 65.469 kb on - strand, within EX31_RS12005 at 65.469 kb on - strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.683 kb on + strand at 65.684 kb on - strand at 65.716 kb on + strand at 65.716 kb on + strand at 65.717 kb on - strand at 65.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 62,422 - +0.7 62,452 - +0.1 62,452 - +0.8 62,452 - -0.4 62,452 - +0.1 62,524 + +0.9 62,524 + -0.4 62,524 + +1.9 62,525 - -0.7 62,525 - -0.1 62,525 - -0.1 62,525 - +0.2 62,525 - +0.5 62,535 + -0.4 62,535 + +1.2 62,536 - +0.7 62,536 - +2.0 62,536 - +0.5 62,536 - -0.6 62,536 - +1.0 62,536 - +0.4 62,536 - +0.5 62,536 - -0.4 62,536 - +0.5 62,577 + -0.1 62,577 + +0.8 62,577 + +0.4 62,577 + +0.7 62,577 + +0.9 62,577 + -0.5 62,577 + -0.5 62,577 + +0.2 62,578 - -1.0 62,578 - +0.6 62,595 + +0.6 62,595 + +0.4 62,595 + +0.4 62,595 + -0.3 62,595 + +0.0 62,656 - -0.9 63,369 + -0.2 63,369 + +0.5 63,370 - +1.6 63,410 - +0.7 63,410 - -0.8 63,410 - -2.0 63,604 - EX31_RS12000 0.16 -0.9 63,604 - EX31_RS12000 0.16 +1.9 63,604 - EX31_RS12000 0.16 +0.1 63,620 + EX31_RS12000 0.17 +0.5 63,703 + EX31_RS12000 0.23 +0.0 63,703 + EX31_RS12000 0.23 +1.1 63,703 + EX31_RS12000 0.23 +0.3 63,704 - EX31_RS12000 0.23 -0.3 63,704 - EX31_RS12000 0.23 -0.4 63,704 - EX31_RS12000 0.23 +0.7 63,704 - EX31_RS12000 0.23 -1.0 63,704 - EX31_RS12000 0.23 -0.1 63,736 - EX31_RS12000 0.25 +1.3 63,736 - EX31_RS12000 0.25 -0.0 63,902 + EX31_RS12000 0.37 +1.7 63,902 + EX31_RS12000 0.37 +0.5 63,902 + EX31_RS12000 0.37 -0.3 63,902 + EX31_RS12000 0.37 +0.0 63,902 + EX31_RS12000 0.37 -0.0 63,902 + EX31_RS12000 0.37 -0.0 63,902 + EX31_RS12000 0.37 -0.6 63,903 - EX31_RS12000 0.37 -0.0 63,903 - EX31_RS12000 0.37 +0.1 63,903 - EX31_RS12000 0.37 -1.4 63,903 - EX31_RS12000 0.37 +0.8 63,903 - EX31_RS12000 0.37 +0.7 63,903 - EX31_RS12000 0.37 -0.9 63,949 - EX31_RS12000 0.40 +0.1 63,949 - EX31_RS12000 0.40 -0.5 63,949 - EX31_RS12000 0.40 +0.6 63,949 - EX31_RS12000 0.40 +0.7 63,961 - EX31_RS12000 0.41 +1.0 64,046 + EX31_RS12000 0.47 +0.2 64,046 + EX31_RS12000 0.47 +0.3 64,046 + EX31_RS12000 0.47 -0.1 64,046 + EX31_RS12000 0.47 -2.8 64,046 + EX31_RS12000 0.47 -1.8 64,046 + EX31_RS12000 0.47 -0.1 64,047 - EX31_RS12000 0.47 +0.4 64,047 - EX31_RS12000 0.47 -0.4 64,047 - EX31_RS12000 0.47 -0.4 64,292 - EX31_RS12000 0.64 -0.5 64,382 + EX31_RS12000 0.70 +0.2 64,382 + EX31_RS12000 0.70 -0.1 64,382 + EX31_RS12000 0.70 -0.2 64,404 + EX31_RS12000 0.72 -0.8 64,404 + EX31_RS12000 0.72 -0.9 64,404 + EX31_RS12000 0.72 +0.1 64,404 + EX31_RS12000 0.72 +0.1 64,404 + EX31_RS12000 0.72 +0.7 64,404 + EX31_RS12000 0.72 -0.3 64,405 - EX31_RS12000 0.72 +0.1 64,405 - EX31_RS12000 0.72 -0.2 64,405 - EX31_RS12000 0.72 -2.0 64,405 - EX31_RS12000 0.72 -1.0 64,405 - EX31_RS12000 0.72 +0.1 64,405 - EX31_RS12000 0.72 -0.1 64,405 - EX31_RS12000 0.72 +0.7 64,405 - EX31_RS12000 0.72 -0.7 64,435 + EX31_RS12000 0.74 -0.6 64,435 + EX31_RS12000 0.74 -2.6 64,435 + EX31_RS12000 0.74 +1.2 64,435 + EX31_RS12000 0.74 -0.4 64,435 + EX31_RS12000 0.74 +0.8 64,435 + EX31_RS12000 0.74 +0.8 64,436 - EX31_RS12000 0.74 +1.4 64,436 - EX31_RS12000 0.74 -0.2 64,436 - EX31_RS12000 0.74 +0.4 64,529 + EX31_RS12000 0.81 +0.7 64,529 + EX31_RS12000 0.81 -0.5 64,530 - EX31_RS12000 0.81 -0.1 64,530 - EX31_RS12000 0.81 +0.5 64,530 - EX31_RS12000 0.81 -0.3 64,607 + EX31_RS12000 0.86 -0.2 64,695 + -0.2 64,695 + -0.1 64,696 - +0.6 64,795 - +0.4 64,838 + -1.9 64,838 + +0.1 64,902 + +0.9 64,906 + -0.5 64,906 + -0.8 64,906 + +1.1 64,907 - -0.9 64,907 - +0.9 64,907 - -0.5 65,063 + EX31_RS12005 0.19 -0.1 65,064 - EX31_RS12005 0.19 -1.4 65,124 + EX31_RS12005 0.26 -0.1 65,124 + EX31_RS12005 0.26 +1.4 65,131 + EX31_RS12005 0.27 -0.6 65,140 + EX31_RS12005 0.28 -0.0 65,140 + EX31_RS12005 0.28 +0.5 65,141 - EX31_RS12005 0.28 -1.4 65,141 - EX31_RS12005 0.28 -0.9 65,141 - EX31_RS12005 0.28 -0.4 65,141 - EX31_RS12005 0.28 -0.6 65,182 - EX31_RS12005 0.33 -0.2 65,182 - EX31_RS12005 0.33 +0.0 65,390 - EX31_RS12005 0.57 -0.4 65,469 - EX31_RS12005 0.66 +1.9 65,469 - EX31_RS12005 0.66 +0.1 65,661 + EX31_RS12005 0.89 +0.9 65,661 + EX31_RS12005 0.89 -0.3 65,661 + EX31_RS12005 0.89 -1.3 65,662 - EX31_RS12005 0.89 -1.4 65,662 - EX31_RS12005 0.89 -0.6 65,662 - EX31_RS12005 0.89 -2.4 65,662 - EX31_RS12005 0.89 -0.9 65,683 + -1.1 65,684 - -0.3 65,716 + +0.3 65,716 + +1.7 65,717 - -1.6 65,799 - -0.2
Or see this region's nucleotide sequence