Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09215 and EX31_RS09220 are separated by 2 nucleotides EX31_RS09220 and EX31_RS09225 are separated by 64 nucleotides
EX31_RS09215: EX31_RS09215 - MFS transporter, at 40,400 to 41,719
_RS09215
EX31_RS09220: EX31_RS09220 - MurR/RpiR family transcriptional regulator, at 41,722 to 42,576
_RS09220
EX31_RS09225: EX31_RS09225 - iron-containing alcohol dehydrogenase family protein, at 42,641 to 43,729
_RS09225
Position (kb) 41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 40.737 kb on - strand, within EX31_RS09215 at 40.767 kb on + strand, within EX31_RS09215 at 40.867 kb on + strand, within EX31_RS09215 at 40.871 kb on + strand, within EX31_RS09215 at 40.891 kb on + strand, within EX31_RS09215 at 40.985 kb on - strand, within EX31_RS09215 at 41.027 kb on + strand, within EX31_RS09215 at 41.027 kb on + strand, within EX31_RS09215 at 41.140 kb on + strand, within EX31_RS09215 at 41.141 kb on - strand, within EX31_RS09215 at 41.186 kb on + strand, within EX31_RS09215 at 41.188 kb on + strand, within EX31_RS09215 at 41.188 kb on + strand, within EX31_RS09215 at 41.214 kb on - strand, within EX31_RS09215 at 41.293 kb on + strand, within EX31_RS09215 at 41.523 kb on - strand, within EX31_RS09215 at 41.544 kb on - strand, within EX31_RS09215 at 41.584 kb on + strand, within EX31_RS09215 at 41.634 kb on - strand at 41.636 kb on + strand at 41.637 kb on - strand at 41.637 kb on - strand at 41.637 kb on - strand at 41.637 kb on - strand at 41.637 kb on - strand at 41.637 kb on - strand at 41.701 kb on - strand at 41.717 kb on + strand at 42.135 kb on + strand, within EX31_RS09220 at 42.139 kb on + strand, within EX31_RS09220 at 42.604 kb on + strand at 42.605 kb on - strand at 42.605 kb on - strand at 42.605 kb on - strand at 42.605 kb on - strand at 42.606 kb on + strand at 42.607 kb on - strand at 42.607 kb on - strand at 42.607 kb on - strand at 42.607 kb on - strand at 42.676 kb on - strand at 42.697 kb on + strand at 42.697 kb on + strand at 42.818 kb on + strand, within EX31_RS09225 at 42.819 kb on - strand, within EX31_RS09225 at 42.819 kb on - strand, within EX31_RS09225 at 43.412 kb on + strand, within EX31_RS09225 at 43.412 kb on + strand, within EX31_RS09225 at 43.413 kb on - strand, within EX31_RS09225 at 43.413 kb on - strand, within EX31_RS09225 at 43.447 kb on + strand, within EX31_RS09225
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 40,737 - EX31_RS09215 0.26 -0.7 40,767 + EX31_RS09215 0.28 +0.2 40,867 + EX31_RS09215 0.35 -0.1 40,871 + EX31_RS09215 0.36 -0.7 40,891 + EX31_RS09215 0.37 +1.5 40,985 - EX31_RS09215 0.44 +0.8 41,027 + EX31_RS09215 0.47 -0.7 41,027 + EX31_RS09215 0.47 -0.7 41,140 + EX31_RS09215 0.56 -0.3 41,141 - EX31_RS09215 0.56 -0.5 41,186 + EX31_RS09215 0.60 -0.9 41,188 + EX31_RS09215 0.60 -0.1 41,188 + EX31_RS09215 0.60 -0.7 41,214 - EX31_RS09215 0.62 +0.1 41,293 + EX31_RS09215 0.68 +0.9 41,523 - EX31_RS09215 0.85 +0.3 41,544 - EX31_RS09215 0.87 +2.9 41,584 + EX31_RS09215 0.90 +0.6 41,634 - -2.4 41,636 + +1.8 41,637 - +0.0 41,637 - +2.6 41,637 - -1.0 41,637 - +1.1 41,637 - -1.4 41,637 - -1.4 41,701 - -0.4 41,717 + -0.2 42,135 + EX31_RS09220 0.48 -0.5 42,139 + EX31_RS09220 0.49 -1.1 42,604 + -1.7 42,605 - +0.0 42,605 - +0.2 42,605 - -0.3 42,605 - +0.0 42,606 + +1.2 42,607 - +1.9 42,607 - +0.9 42,607 - -0.2 42,607 - -0.3 42,676 - +1.4 42,697 + +1.3 42,697 + -1.4 42,818 + EX31_RS09225 0.16 +0.3 42,819 - EX31_RS09225 0.16 -1.8 42,819 - EX31_RS09225 0.16 -0.4 43,412 + EX31_RS09225 0.71 -1.1 43,412 + EX31_RS09225 0.71 +0.9 43,413 - EX31_RS09225 0.71 +1.3 43,413 - EX31_RS09225 0.71 -0.3 43,447 + EX31_RS09225 0.74 +0.9
Or see this region's nucleotide sequence