Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07175 and EX31_RS07180 are separated by 114 nucleotides EX31_RS07180 and EX31_RS07185 are separated by 214 nucleotides EX31_RS07185 and EX31_RS07190 are separated by 119 nucleotides
EX31_RS07175: EX31_RS07175 - FAD:protein FMN transferase ApbE, at 231,986 to 233,026
_RS07175
EX31_RS07180: EX31_RS07180 - DNA endonuclease SmrA, at 233,141 to 233,707
_RS07180
EX31_RS07185: EX31_RS07185 - mannosyl-3-phosphoglycerate phosphatase-related protein, at 233,922 to 234,737
_RS07185
EX31_RS07190: EX31_RS07190 - glycosyl transferase, at 234,857 to 236,077
_RS07190
Position (kb) 233
234
235 Strain fitness (log2 ratio) -2
-1
0
1
2 at 232.949 kb on - strand at 232.968 kb on - strand at 232.968 kb on - strand at 232.997 kb on - strand at 232.997 kb on - strand at 232.997 kb on - strand at 233.008 kb on - strand at 233.033 kb on - strand at 233.033 kb on - strand at 233.033 kb on - strand at 233.069 kb on - strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.212 kb on - strand, within EX31_RS07180 at 233.294 kb on - strand, within EX31_RS07180 at 233.296 kb on - strand, within EX31_RS07180 at 233.498 kb on + strand, within EX31_RS07180 at 233.499 kb on - strand, within EX31_RS07180 at 233.499 kb on - strand, within EX31_RS07180 at 233.515 kb on - strand, within EX31_RS07180 at 233.577 kb on - strand, within EX31_RS07180 at 233.632 kb on - strand, within EX31_RS07180 at 233.664 kb on - strand at 233.664 kb on - strand at 233.714 kb on - strand at 233.842 kb on + strand at 233.843 kb on - strand at 233.844 kb on + strand at 233.845 kb on - strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.851 kb on - strand at 233.851 kb on - strand at 233.851 kb on - strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.855 kb on - strand at 233.855 kb on - strand at 233.855 kb on - strand at 233.893 kb on - strand at 233.949 kb on - strand at 234.056 kb on - strand, within EX31_RS07185 at 234.091 kb on + strand, within EX31_RS07185 at 234.126 kb on - strand, within EX31_RS07185 at 234.242 kb on - strand, within EX31_RS07185 at 234.463 kb on + strand, within EX31_RS07185 at 234.463 kb on + strand, within EX31_RS07185 at 234.463 kb on + strand, within EX31_RS07185 at 234.463 kb on + strand, within EX31_RS07185 at 234.464 kb on - strand, within EX31_RS07185 at 234.544 kb on + strand, within EX31_RS07185 at 234.545 kb on - strand, within EX31_RS07185 at 234.545 kb on - strand, within EX31_RS07185 at 234.550 kb on + strand, within EX31_RS07185 at 234.550 kb on + strand, within EX31_RS07185 at 234.551 kb on - strand, within EX31_RS07185 at 234.551 kb on - strand, within EX31_RS07185 at 234.569 kb on + strand, within EX31_RS07185 at 234.569 kb on + strand, within EX31_RS07185 at 234.577 kb on + strand, within EX31_RS07185 at 234.577 kb on + strand, within EX31_RS07185 at 234.577 kb on + strand, within EX31_RS07185 at 234.600 kb on + strand, within EX31_RS07185 at 234.601 kb on - strand, within EX31_RS07185 at 234.722 kb on + strand at 234.723 kb on - strand at 234.723 kb on - strand at 234.725 kb on + strand at 234.768 kb on + strand at 234.834 kb on - strand at 234.857 kb on - strand at 234.886 kb on + strand at 234.890 kb on - strand at 234.972 kb on + strand at 234.975 kb on + strand at 235.074 kb on + strand, within EX31_RS07190 at 235.258 kb on + strand, within EX31_RS07190 at 235.259 kb on - strand, within EX31_RS07190 at 235.259 kb on - strand, within EX31_RS07190 at 235.502 kb on + strand, within EX31_RS07190 at 235.627 kb on + strand, within EX31_RS07190 at 235.627 kb on + strand, within EX31_RS07190 at 235.628 kb on - strand, within EX31_RS07190 at 235.628 kb on - strand, within EX31_RS07190 at 235.671 kb on - strand, within EX31_RS07190 at 235.678 kb on + strand, within EX31_RS07190 at 235.711 kb on + strand, within EX31_RS07190 at 235.712 kb on - strand, within EX31_RS07190 at 235.712 kb on - strand, within EX31_RS07190 at 235.733 kb on - strand, within EX31_RS07190
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 232,949 - -0.5 232,968 - +1.4 232,968 - +1.3 232,997 - -0.2 232,997 - -1.9 232,997 - +0.3 233,008 - +0.4 233,033 - -1.9 233,033 - -0.0 233,033 - -1.6 233,069 - -0.7 233,093 + -0.0 233,093 + +2.0 233,093 + +2.0 233,093 + -0.4 233,212 - EX31_RS07180 0.13 -1.4 233,294 - EX31_RS07180 0.27 +1.5 233,296 - EX31_RS07180 0.27 +0.7 233,498 + EX31_RS07180 0.63 +1.0 233,499 - EX31_RS07180 0.63 -0.1 233,499 - EX31_RS07180 0.63 -0.2 233,515 - EX31_RS07180 0.66 -2.0 233,577 - EX31_RS07180 0.77 +2.0 233,632 - EX31_RS07180 0.87 -0.8 233,664 - +2.0 233,664 - +1.0 233,714 - -0.8 233,842 + -1.0 233,843 - -0.0 233,844 + +1.0 233,845 - +1.7 233,848 + +1.3 233,848 + +0.5 233,848 + +0.6 233,848 + +0.1 233,848 + -1.0 233,848 + +0.5 233,849 - -0.0 233,849 - +0.2 233,849 - -0.6 233,849 - -0.0 233,849 - -1.5 233,849 - +1.1 233,849 - +1.0 233,849 - -1.0 233,850 + -0.4 233,850 + +0.9 233,850 + +1.8 233,850 + +0.8 233,850 + -1.8 233,850 + +2.4 233,851 - +0.8 233,851 - +0.4 233,851 - +0.5 233,854 + -0.1 233,854 + -0.3 233,855 - -1.0 233,855 - +0.8 233,855 - -0.4 233,893 - +1.2 233,949 - +1.8 234,056 - EX31_RS07185 0.16 -0.8 234,091 + EX31_RS07185 0.21 +2.0 234,126 - EX31_RS07185 0.25 -0.4 234,242 - EX31_RS07185 0.39 -0.6 234,463 + EX31_RS07185 0.66 -0.0 234,463 + EX31_RS07185 0.66 -2.0 234,463 + EX31_RS07185 0.66 -2.1 234,463 + EX31_RS07185 0.66 -1.2 234,464 - EX31_RS07185 0.66 -0.6 234,544 + EX31_RS07185 0.76 +1.4 234,545 - EX31_RS07185 0.76 -1.0 234,545 - EX31_RS07185 0.76 -0.0 234,550 + EX31_RS07185 0.77 +1.0 234,550 + EX31_RS07185 0.77 +2.0 234,551 - EX31_RS07185 0.77 -0.9 234,551 - EX31_RS07185 0.77 -2.4 234,569 + EX31_RS07185 0.79 -0.2 234,569 + EX31_RS07185 0.79 -1.0 234,577 + EX31_RS07185 0.80 -0.4 234,577 + EX31_RS07185 0.80 +0.4 234,577 + EX31_RS07185 0.80 -0.6 234,600 + EX31_RS07185 0.83 +1.0 234,601 - EX31_RS07185 0.83 -0.3 234,722 + -1.8 234,723 - +0.4 234,723 - -0.5 234,725 + -0.6 234,768 + -1.0 234,834 - +0.9 234,857 - +0.2 234,886 + +2.0 234,890 - +1.7 234,972 + +0.3 234,975 + -0.6 235,074 + EX31_RS07190 0.18 -0.0 235,258 + EX31_RS07190 0.33 -0.4 235,259 - EX31_RS07190 0.33 +0.6 235,259 - EX31_RS07190 0.33 +1.6 235,502 + EX31_RS07190 0.53 +0.8 235,627 + EX31_RS07190 0.63 -0.0 235,627 + EX31_RS07190 0.63 +1.3 235,628 - EX31_RS07190 0.63 +0.5 235,628 - EX31_RS07190 0.63 -0.3 235,671 - EX31_RS07190 0.67 +0.2 235,678 + EX31_RS07190 0.67 -0.6 235,711 + EX31_RS07190 0.70 +0.5 235,712 - EX31_RS07190 0.70 +0.2 235,712 - EX31_RS07190 0.70 +1.0 235,733 - EX31_RS07190 0.72 -1.0
Or see this region's nucleotide sequence