Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01705 and EX31_RS01710 overlap by 4 nucleotides EX31_RS01710 and EX31_RS01715 overlap by 4 nucleotides EX31_RS01715 and EX31_RS01720 overlap by 19 nucleotides EX31_RS01720 and EX31_RS01730 are separated by 530 nucleotides
EX31_RS01705: EX31_RS01705 - site-specific DNA-methyltransferase, at 373,943 to 375,067
_RS01705
EX31_RS01710: EX31_RS01710 - hypothetical protein, at 375,064 to 375,405
_RS01710
EX31_RS01715: EX31_RS01715 - DNA replication protein, at 375,402 to 376,106
_RS01715
EX31_RS01720: EX31_RS01720 - helix-turn-helix domain-containing protein, at 376,088 to 376,528
_RS01720
EX31_RS01730: EX31_RS01730 - DUF4222 domain-containing protein, at 377,059 to 377,214
_RS01730
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 374.419 kb on + strand, within EX31_RS01705 at 374.465 kb on + strand, within EX31_RS01705 at 374.466 kb on - strand, within EX31_RS01705 at 374.552 kb on - strand, within EX31_RS01705 at 374.552 kb on - strand, within EX31_RS01705 at 374.595 kb on - strand, within EX31_RS01705 at 374.623 kb on + strand, within EX31_RS01705 at 374.669 kb on + strand, within EX31_RS01705 at 374.670 kb on - strand, within EX31_RS01705 at 374.788 kb on - strand, within EX31_RS01705 at 374.831 kb on + strand, within EX31_RS01705 at 374.831 kb on + strand, within EX31_RS01705 at 374.831 kb on + strand, within EX31_RS01705 at 374.831 kb on + strand, within EX31_RS01705 at 374.831 kb on + strand, within EX31_RS01705 at 374.832 kb on - strand, within EX31_RS01705 at 374.832 kb on - strand, within EX31_RS01705 at 374.832 kb on - strand, within EX31_RS01705 at 374.832 kb on - strand, within EX31_RS01705 at 374.833 kb on + strand, within EX31_RS01705 at 374.834 kb on - strand, within EX31_RS01705 at 374.853 kb on + strand, within EX31_RS01705 at 374.853 kb on + strand, within EX31_RS01705 at 374.854 kb on - strand, within EX31_RS01705 at 374.857 kb on + strand, within EX31_RS01705 at 374.858 kb on - strand, within EX31_RS01705 at 374.858 kb on - strand, within EX31_RS01705 at 374.858 kb on - strand, within EX31_RS01705 at 374.896 kb on + strand, within EX31_RS01705 at 374.897 kb on - strand, within EX31_RS01705 at 375.061 kb on - strand at 375.061 kb on - strand at 375.089 kb on - strand at 375.089 kb on - strand at 375.206 kb on + strand, within EX31_RS01710 at 375.369 kb on + strand, within EX31_RS01710 at 375.369 kb on + strand, within EX31_RS01710 at 375.370 kb on - strand, within EX31_RS01710 at 375.382 kb on - strand at 375.385 kb on + strand at 375.386 kb on - strand at 375.442 kb on + strand at 375.442 kb on + strand at 375.545 kb on + strand, within EX31_RS01715 at 375.545 kb on + strand, within EX31_RS01715 at 375.590 kb on - strand, within EX31_RS01715 at 375.764 kb on + strand, within EX31_RS01715 at 375.765 kb on - strand, within EX31_RS01715 at 375.765 kb on - strand, within EX31_RS01715 at 375.765 kb on - strand, within EX31_RS01715 at 375.765 kb on - strand, within EX31_RS01715 at 376.067 kb on + strand at 376.383 kb on + strand, within EX31_RS01720 at 376.490 kb on + strand at 376.525 kb on + strand at 376.525 kb on + strand at 376.736 kb on - strand at 376.941 kb on - strand at 376.993 kb on - strand at 377.031 kb on - strand at 377.042 kb on + strand at 377.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 374,419 + EX31_RS01705 0.42 +0.7 374,465 + EX31_RS01705 0.46 +0.4 374,466 - EX31_RS01705 0.46 +0.4 374,552 - EX31_RS01705 0.54 +2.0 374,552 - EX31_RS01705 0.54 +2.0 374,595 - EX31_RS01705 0.58 +0.0 374,623 + EX31_RS01705 0.60 +3.0 374,669 + EX31_RS01705 0.65 +0.7 374,670 - EX31_RS01705 0.65 +0.2 374,788 - EX31_RS01705 0.75 +0.3 374,831 + EX31_RS01705 0.79 +0.1 374,831 + EX31_RS01705 0.79 -0.3 374,831 + EX31_RS01705 0.79 +2.9 374,831 + EX31_RS01705 0.79 +0.2 374,831 + EX31_RS01705 0.79 -1.0 374,832 - EX31_RS01705 0.79 +0.4 374,832 - EX31_RS01705 0.79 -0.6 374,832 - EX31_RS01705 0.79 +0.2 374,832 - EX31_RS01705 0.79 +0.0 374,833 + EX31_RS01705 0.79 -0.8 374,834 - EX31_RS01705 0.79 +2.0 374,853 + EX31_RS01705 0.81 +0.6 374,853 + EX31_RS01705 0.81 +0.2 374,854 - EX31_RS01705 0.81 -1.0 374,857 + EX31_RS01705 0.81 -0.6 374,858 - EX31_RS01705 0.81 +1.8 374,858 - EX31_RS01705 0.81 -1.0 374,858 - EX31_RS01705 0.81 +1.5 374,896 + EX31_RS01705 0.85 +1.7 374,897 - EX31_RS01705 0.85 +2.3 375,061 - +0.5 375,061 - +1.6 375,089 - +0.0 375,089 - +0.6 375,206 + EX31_RS01710 0.42 -0.8 375,369 + EX31_RS01710 0.89 +1.5 375,369 + EX31_RS01710 0.89 -2.4 375,370 - EX31_RS01710 0.89 -1.1 375,382 - +0.7 375,385 + +1.8 375,386 - +0.1 375,442 + -0.4 375,442 + +0.4 375,545 + EX31_RS01715 0.20 -0.9 375,545 + EX31_RS01715 0.20 -0.8 375,590 - EX31_RS01715 0.27 -0.8 375,764 + EX31_RS01715 0.51 +3.6 375,765 - EX31_RS01715 0.51 +1.6 375,765 - EX31_RS01715 0.51 -0.1 375,765 - EX31_RS01715 0.51 +0.0 375,765 - EX31_RS01715 0.51 +0.0 376,067 + -0.1 376,383 + EX31_RS01720 0.67 +0.3 376,490 + -0.3 376,525 + -1.0 376,525 + -0.4 376,736 - -0.6 376,941 - +2.0 376,993 - +1.1 377,031 - +1.9 377,042 + +0.0 377,043 - +2.0
Or see this region's nucleotide sequence