Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02215 and MMP_RS02220 are separated by 207 nucleotides MMP_RS02220 and MMP_RS02225 are separated by 19 nucleotides MMP_RS02225 and MMP_RS02230 are separated by 142 nucleotides
MMP_RS02215: MMP_RS02215 - ECF transporter S component, at 417,866 to 418,462
_RS02215
MMP_RS02220: MMP_RS02220 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase, at 418,670 to 419,395
_RS02220
MMP_RS02225: MMP_RS02225 - carbohydrate kinase family protein, at 419,415 to 420,323
_RS02225
MMP_RS02230: MMP_RS02230 - sodium-dependent transporter, at 420,466 to 421,977
_RS02230
Position (kb) 419
420
421 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 418.542 kb on - strand at 418.542 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.586 kb on - strand at 418.586 kb on - strand at 418.625 kb on - strand at 418.674 kb on - strand at 419.176 kb on + strand at 419.325 kb on - strand at 419.463 kb on - strand at 419.594 kb on + strand, within MMP_RS02225 at 419.640 kb on - strand, within MMP_RS02225 at 419.812 kb on - strand, within MMP_RS02225 at 419.813 kb on + strand, within MMP_RS02225 at 419.914 kb on - strand, within MMP_RS02225 at 419.967 kb on + strand, within MMP_RS02225 at 420.047 kb on + strand, within MMP_RS02225 at 420.080 kb on + strand, within MMP_RS02225 at 420.081 kb on - strand, within MMP_RS02225 at 420.352 kb on - strand at 420.408 kb on + strand at 420.414 kb on + strand at 420.414 kb on + strand at 420.750 kb on + strand, within MMP_RS02230 at 420.792 kb on + strand, within MMP_RS02230 at 420.793 kb on - strand, within MMP_RS02230 at 420.855 kb on - strand, within MMP_RS02230 at 420.898 kb on + strand, within MMP_RS02230 at 420.930 kb on + strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 421.078 kb on - strand, within MMP_RS02230 at 421.107 kb on + strand, within MMP_RS02230 at 421.116 kb on - strand, within MMP_RS02230
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 20 generations remove 418,542 - +0.2 418,542 - -0.4 418,582 - -5.6 418,582 - -5.1 418,582 - -6.1 418,582 - -5.6 418,582 - -6.5 418,586 - -4.2 418,586 - -4.8 418,625 - -3.9 418,674 - -4.5 419,176 + -4.8 419,325 - -3.9 419,463 - -1.6 419,594 + MMP_RS02225 0.20 -1.7 419,640 - MMP_RS02225 0.25 -1.8 419,812 - MMP_RS02225 0.44 -0.9 419,813 + MMP_RS02225 0.44 -1.5 419,914 - MMP_RS02225 0.55 -1.6 419,967 + MMP_RS02225 0.61 -1.2 420,047 + MMP_RS02225 0.70 -1.6 420,080 + MMP_RS02225 0.73 -1.1 420,081 - MMP_RS02225 0.73 -2.0 420,352 - +0.6 420,408 + -0.8 420,414 + -1.5 420,414 + -0.6 420,750 + MMP_RS02230 0.19 -2.1 420,792 + MMP_RS02230 0.22 -1.7 420,793 - MMP_RS02230 0.22 -1.4 420,855 - MMP_RS02230 0.26 -2.2 420,898 + MMP_RS02230 0.29 -1.7 420,930 + MMP_RS02230 0.31 -1.7 420,943 - MMP_RS02230 0.32 -2.3 420,943 - MMP_RS02230 0.32 -0.8 420,943 - MMP_RS02230 0.32 -2.9 421,078 - MMP_RS02230 0.40 -2.2 421,107 + MMP_RS02230 0.42 -1.9 421,116 - MMP_RS02230 0.43 -1.2
Or see this region's nucleotide sequence