Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22245 and EX31_RS22250 are separated by 205 nucleotides EX31_RS22250 and EX31_RS22255 are separated by 191 nucleotides
EX31_RS22245: EX31_RS22245 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 199,002 to 199,625
_RS22245
EX31_RS22250: EX31_RS22250 - ATP-dependent protease ATP-binding subunit ClpX, at 199,831 to 201,105
_RS22250
EX31_RS22255: EX31_RS22255 - endopeptidase La, at 201,297 to 203,651
_RS22255
Position (kb) 199
200
201
202 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 198.846 kb on - strand at 198.852 kb on + strand at 198.852 kb on + strand at 198.853 kb on - strand at 198.853 kb on - strand at 198.869 kb on + strand at 198.873 kb on + strand at 198.873 kb on + strand at 198.873 kb on + strand at 198.887 kb on + strand at 198.888 kb on - strand at 198.905 kb on + strand at 198.905 kb on + strand at 198.906 kb on - strand at 198.931 kb on + strand at 198.931 kb on + strand at 198.932 kb on - strand at 198.932 kb on - strand at 198.945 kb on + strand at 198.954 kb on + strand at 198.954 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.990 kb on + strand at 198.990 kb on + strand at 199.001 kb on + strand at 199.001 kb on + strand at 199.001 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.009 kb on - strand at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.157 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.229 kb on + strand, within EX31_RS22245 at 199.262 kb on + strand, within EX31_RS22245 at 199.272 kb on + strand, within EX31_RS22245 at 199.511 kb on + strand, within EX31_RS22245 at 199.534 kb on + strand, within EX31_RS22245 at 199.582 kb on + strand at 199.597 kb on + strand at 199.597 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.660 kb on - strand at 199.660 kb on - strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.723 kb on + strand at 199.723 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.767 kb on - strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.788 kb on + strand at 199.839 kb on + strand at 199.839 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.908 kb on + strand at 199.933 kb on + strand at 199.933 kb on + strand at 199.933 kb on + strand at 199.934 kb on - strand at 199.935 kb on + strand at 199.958 kb on + strand at 199.962 kb on + strand, within EX31_RS22250 at 199.962 kb on + strand, within EX31_RS22250 at 200.059 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.118 kb on + strand, within EX31_RS22250 at 200.118 kb on + strand, within EX31_RS22250 at 200.169 kb on + strand, within EX31_RS22250 at 200.250 kb on + strand, within EX31_RS22250 at 200.250 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.450 kb on + strand, within EX31_RS22250 at 200.450 kb on + strand, within EX31_RS22250 at 200.472 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.547 kb on + strand, within EX31_RS22250 at 200.625 kb on + strand, within EX31_RS22250 at 200.687 kb on + strand, within EX31_RS22250 at 200.744 kb on + strand, within EX31_RS22250 at 200.744 kb on + strand, within EX31_RS22250 at 200.744 kb on + strand, within EX31_RS22250 at 200.839 kb on + strand, within EX31_RS22250 at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 200.983 kb on + strand at 201.067 kb on + strand at 201.067 kb on + strand at 201.108 kb on - strand at 201.128 kb on + strand at 201.177 kb on + strand at 201.206 kb on + strand at 201.206 kb on + strand at 201.206 kb on + strand at 201.206 kb on + strand at 201.206 kb on + strand at 201.208 kb on + strand at 201.208 kb on + strand at 201.208 kb on + strand at 201.208 kb on + strand at 201.208 kb on + strand at 201.208 kb on + strand at 201.209 kb on - strand at 201.209 kb on - strand at 201.209 kb on - strand at 201.278 kb on + strand at 201.283 kb on + strand at 201.283 kb on + strand at 201.283 kb on + strand at 201.283 kb on + strand at 201.296 kb on + strand at 201.296 kb on + strand at 201.526 kb on + strand at 201.529 kb on + strand at 201.529 kb on + strand at 201.529 kb on + strand at 201.529 kb on + strand at 201.529 kb on + strand at 201.580 kb on + strand, within EX31_RS22255 at 201.580 kb on + strand, within EX31_RS22255 at 201.580 kb on + strand, within EX31_RS22255 at 201.593 kb on + strand, within EX31_RS22255 at 201.594 kb on - strand, within EX31_RS22255 at 201.692 kb on + strand, within EX31_RS22255 at 201.717 kb on + strand, within EX31_RS22255 at 201.717 kb on + strand, within EX31_RS22255 at 201.717 kb on + strand, within EX31_RS22255 at 201.717 kb on + strand, within EX31_RS22255 at 201.717 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.812 kb on + strand, within EX31_RS22255 at 201.813 kb on - strand, within EX31_RS22255 at 201.879 kb on + strand, within EX31_RS22255 at 201.981 kb on + strand, within EX31_RS22255 at 201.981 kb on + strand, within EX31_RS22255 at 201.981 kb on + strand, within EX31_RS22255 at 201.981 kb on + strand, within EX31_RS22255 at 201.981 kb on + strand, within EX31_RS22255
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 198,846 - -1.2 198,852 + +0.3 198,852 + -1.0 198,853 - -0.5 198,853 - -1.0 198,869 + +0.7 198,873 + -0.4 198,873 + -1.1 198,873 + -1.9 198,887 + -0.8 198,888 - +0.8 198,905 + -1.3 198,905 + +1.0 198,906 - -1.3 198,931 + -0.4 198,931 + -0.2 198,932 - +1.1 198,932 - -0.5 198,945 + +0.9 198,954 + +0.6 198,954 + +0.3 198,987 + -0.7 198,987 + +0.8 198,987 + -0.4 198,987 + +1.0 198,990 + +1.4 198,990 + -1.6 199,001 + -1.0 199,001 + -2.1 199,001 + -2.0 199,008 + -0.9 199,008 + -1.2 199,008 + -1.2 199,008 + +1.0 199,008 + -2.0 199,009 - -1.3 199,124 + EX31_RS22245 0.20 -1.0 199,124 + EX31_RS22245 0.20 -0.7 199,124 + EX31_RS22245 0.20 -1.8 199,124 + EX31_RS22245 0.20 -1.5 199,124 + EX31_RS22245 0.20 -2.8 199,157 + EX31_RS22245 0.25 -0.2 199,221 + EX31_RS22245 0.35 -1.6 199,221 + EX31_RS22245 0.35 -1.2 199,221 + EX31_RS22245 0.35 -0.2 199,227 + EX31_RS22245 0.36 -1.0 199,227 + EX31_RS22245 0.36 -1.0 199,227 + EX31_RS22245 0.36 -0.2 199,227 + EX31_RS22245 0.36 -0.7 199,229 + EX31_RS22245 0.36 -2.6 199,262 + EX31_RS22245 0.42 -0.7 199,272 + EX31_RS22245 0.43 -0.5 199,511 + EX31_RS22245 0.82 -2.2 199,534 + EX31_RS22245 0.85 -0.2 199,582 + -1.7 199,597 + -1.7 199,597 + -1.8 199,653 + +1.1 199,653 + +0.0 199,653 + -0.2 199,653 + -0.2 199,653 + +0.4 199,653 + -2.3 199,653 + -0.1 199,653 + -0.7 199,653 + -1.4 199,653 + -0.5 199,653 + -0.4 199,653 + +0.9 199,653 + -0.1 199,653 + -0.8 199,655 + -1.6 199,655 + -0.6 199,655 + -1.2 199,655 + -0.5 199,655 + +0.1 199,655 + +0.1 199,655 + +0.1 199,655 + +0.5 199,655 + +1.0 199,655 + +0.7 199,655 + -0.8 199,655 + -0.6 199,655 + -1.3 199,655 + +1.1 199,655 + -0.2 199,655 + +0.1 199,655 + -0.5 199,655 + +0.7 199,655 + -0.4 199,655 + +0.5 199,656 - -1.7 199,656 - -0.3 199,656 - +2.1 199,656 - -0.5 199,659 + +0.2 199,659 + -2.1 199,659 + -0.6 199,659 + +0.4 199,659 + -0.0 199,659 + -1.1 199,659 + -0.3 199,659 + -0.6 199,659 + +0.6 199,659 + +0.0 199,659 + +1.0 199,659 + +0.9 199,659 + +0.7 199,659 + +0.3 199,659 + -1.1 199,660 - +0.8 199,660 - +0.5 199,661 + -1.0 199,661 + +0.5 199,661 + +0.4 199,661 + -0.8 199,661 + +0.1 199,723 + +1.1 199,723 + -0.3 199,766 + +1.7 199,766 + +0.7 199,766 + -0.8 199,766 + -1.1 199,767 - -1.0 199,769 + -1.2 199,769 + -0.1 199,769 + +0.0 199,769 + +1.3 199,769 + -1.0 199,769 + +0.2 199,769 + -0.9 199,769 + +0.8 199,771 + -2.0 199,771 + +1.4 199,771 + -1.6 199,771 + +0.1 199,771 + +0.2 199,771 + -0.5 199,771 + -2.6 199,788 + -0.3 199,839 + -0.5 199,839 + -0.8 199,870 + -0.5 199,870 + -1.0 199,870 + -0.5 199,908 + -0.2 199,933 + -2.8 199,933 + -1.5 199,933 + -1.9 199,934 - +0.5 199,935 + -0.5 199,958 + +1.1 199,962 + EX31_RS22250 0.10 -1.7 199,962 + EX31_RS22250 0.10 -2.3 200,059 + EX31_RS22250 0.18 -1.8 200,116 + EX31_RS22250 0.22 -0.2 200,116 + EX31_RS22250 0.22 -2.0 200,116 + EX31_RS22250 0.22 -1.2 200,118 + EX31_RS22250 0.23 -0.2 200,118 + EX31_RS22250 0.23 -1.3 200,169 + EX31_RS22250 0.27 -2.4 200,250 + EX31_RS22250 0.33 -0.5 200,250 + EX31_RS22250 0.33 -1.8 200,374 + EX31_RS22250 0.43 -0.6 200,374 + EX31_RS22250 0.43 +0.1 200,374 + EX31_RS22250 0.43 -1.8 200,450 + EX31_RS22250 0.49 -2.0 200,450 + EX31_RS22250 0.49 -1.2 200,472 + EX31_RS22250 0.50 -0.7 200,514 + EX31_RS22250 0.54 -1.5 200,514 + EX31_RS22250 0.54 -0.5 200,514 + EX31_RS22250 0.54 -1.7 200,514 + EX31_RS22250 0.54 -0.7 200,547 + EX31_RS22250 0.56 -0.3 200,625 + EX31_RS22250 0.62 -1.0 200,687 + EX31_RS22250 0.67 -1.7 200,744 + EX31_RS22250 0.72 -1.2 200,744 + EX31_RS22250 0.72 -0.9 200,744 + EX31_RS22250 0.72 -1.0 200,839 + EX31_RS22250 0.79 -2.3 200,983 + -0.7 200,983 + -0.9 200,983 + -1.3 200,983 + +0.1 200,983 + -1.0 200,983 + -1.0 200,983 + -2.6 200,983 + -1.8 200,983 + -0.8 200,983 + -0.9 200,983 + -1.3 200,983 + -0.1 201,067 + -1.1 201,067 + -0.5 201,108 - +1.6 201,128 + -1.2 201,177 + -0.6 201,206 + -0.7 201,206 + -1.4 201,206 + +0.1 201,206 + -0.9 201,206 + -0.6 201,208 + +2.1 201,208 + -0.2 201,208 + +0.8 201,208 + +0.8 201,208 + -0.2 201,208 + -0.4 201,209 - -0.2 201,209 - -0.9 201,209 - +0.1 201,278 + -0.7 201,283 + +0.7 201,283 + +0.0 201,283 + -1.9 201,283 + -1.8 201,296 + +0.2 201,296 + -2.5 201,526 + -0.0 201,529 + -0.5 201,529 + -0.5 201,529 + -1.1 201,529 + +1.6 201,529 + -1.6 201,580 + EX31_RS22255 0.12 -0.7 201,580 + EX31_RS22255 0.12 -1.6 201,580 + EX31_RS22255 0.12 -1.2 201,593 + EX31_RS22255 0.13 -0.0 201,594 - EX31_RS22255 0.13 +2.5 201,692 + EX31_RS22255 0.17 +0.3 201,717 + EX31_RS22255 0.18 -1.2 201,717 + EX31_RS22255 0.18 +0.0 201,717 + EX31_RS22255 0.18 -0.2 201,717 + EX31_RS22255 0.18 -1.6 201,717 + EX31_RS22255 0.18 -0.5 201,812 + EX31_RS22255 0.22 -0.0 201,812 + EX31_RS22255 0.22 +1.3 201,812 + EX31_RS22255 0.22 -0.8 201,812 + EX31_RS22255 0.22 -0.5 201,812 + EX31_RS22255 0.22 +0.4 201,812 + EX31_RS22255 0.22 +0.4 201,812 + EX31_RS22255 0.22 +0.0 201,812 + EX31_RS22255 0.22 -0.0 201,812 + EX31_RS22255 0.22 -0.6 201,812 + EX31_RS22255 0.22 +0.3 201,812 + EX31_RS22255 0.22 -2.4 201,812 + EX31_RS22255 0.22 +0.1 201,813 - EX31_RS22255 0.22 -0.2 201,879 + EX31_RS22255 0.25 -0.3 201,981 + EX31_RS22255 0.29 +1.0 201,981 + EX31_RS22255 0.29 +0.5 201,981 + EX31_RS22255 0.29 -2.6 201,981 + EX31_RS22255 0.29 +0.1 201,981 + EX31_RS22255 0.29 -0.0
Or see this region's nucleotide sequence