Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15355 and EX31_RS15360 are separated by 17 nucleotides EX31_RS15360 and EX31_RS15365 are separated by 46 nucleotides EX31_RS15365 and EX31_RS15370 are separated by 15 nucleotides
EX31_RS15355: EX31_RS15355 - hypothetical protein, at 22,485 to 22,823
_RS15355
EX31_RS15360: EX31_RS15360 - Fe-S cluster assembly protein NifU, at 22,841 to 23,668
_RS15360
EX31_RS15365: EX31_RS15365 - cysteine desulfurase NifS, at 23,715 to 24,920
_RS15365
EX31_RS15370: EX31_RS15370 - homocitrate synthase, at 24,936 to 26,138
_RS15370
Position (kb) 23
24
25 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.747 kb on + strand, within EX31_RS15355 at 22.748 kb on - strand, within EX31_RS15355 at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.800 kb on - strand at 22.800 kb on - strand at 22.835 kb on - strand at 22.850 kb on + strand at 23.198 kb on + strand, within EX31_RS15360 at 23.198 kb on + strand, within EX31_RS15360 at 23.198 kb on + strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.199 kb on - strand, within EX31_RS15360 at 23.201 kb on + strand, within EX31_RS15360 at 23.202 kb on - strand, within EX31_RS15360 at 23.245 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.333 kb on + strand, within EX31_RS15360 at 23.334 kb on - strand, within EX31_RS15360 at 23.632 kb on + strand at 23.633 kb on - strand at 23.633 kb on - strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.642 kb on + strand at 23.643 kb on - strand at 23.643 kb on - strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.644 kb on + strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.645 kb on - strand at 23.700 kb on - strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.727 kb on + strand at 23.787 kb on + strand at 23.799 kb on + strand at 23.898 kb on + strand, within EX31_RS15365 at 23.898 kb on + strand, within EX31_RS15365 at 23.898 kb on + strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.899 kb on - strand, within EX31_RS15365 at 23.900 kb on + strand, within EX31_RS15365 at 23.901 kb on - strand, within EX31_RS15365 at 23.901 kb on - strand, within EX31_RS15365 at 23.913 kb on - strand, within EX31_RS15365 at 23.955 kb on + strand, within EX31_RS15365 at 23.955 kb on + strand, within EX31_RS15365 at 23.956 kb on - strand, within EX31_RS15365 at 23.993 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.057 kb on + strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.058 kb on - strand, within EX31_RS15365 at 24.072 kb on - strand, within EX31_RS15365 at 24.111 kb on + strand, within EX31_RS15365 at 24.111 kb on + strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.112 kb on - strand, within EX31_RS15365 at 24.114 kb on + strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.115 kb on - strand, within EX31_RS15365 at 24.181 kb on + strand, within EX31_RS15365 at 24.181 kb on + strand, within EX31_RS15365 at 24.182 kb on - strand, within EX31_RS15365 at 24.182 kb on - strand, within EX31_RS15365 at 24.317 kb on + strand, within EX31_RS15365 at 24.317 kb on + strand, within EX31_RS15365 at 24.318 kb on - strand, within EX31_RS15365 at 24.351 kb on + strand, within EX31_RS15365 at 24.352 kb on - strand, within EX31_RS15365 at 24.352 kb on - strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.482 kb on + strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.483 kb on - strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.593 kb on + strand, within EX31_RS15365 at 24.594 kb on - strand, within EX31_RS15365 at 24.594 kb on - strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.615 kb on + strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.616 kb on - strand, within EX31_RS15365 at 24.617 kb on + strand, within EX31_RS15365 at 24.617 kb on + strand, within EX31_RS15365 at 24.618 kb on - strand, within EX31_RS15365 at 24.618 kb on - strand, within EX31_RS15365 at 24.647 kb on + strand, within EX31_RS15365 at 24.648 kb on - strand, within EX31_RS15365 at 24.659 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.731 kb on + strand, within EX31_RS15365 at 24.738 kb on + strand, within EX31_RS15365 at 24.738 kb on + strand, within EX31_RS15365 at 24.739 kb on - strand, within EX31_RS15365 at 24.739 kb on - strand, within EX31_RS15365 at 24.739 kb on - strand, within EX31_RS15365 at 24.739 kb on - strand, within EX31_RS15365 at 24.740 kb on + strand, within EX31_RS15365 at 24.740 kb on + strand, within EX31_RS15365 at 24.740 kb on + strand, within EX31_RS15365 at 24.740 kb on + strand, within EX31_RS15365 at 24.741 kb on - strand, within EX31_RS15365 at 24.783 kb on - strand, within EX31_RS15365 at 24.859 kb on - strand at 24.912 kb on + strand at 24.913 kb on - strand at 24.913 kb on - strand at 24.913 kb on - strand at 25.066 kb on + strand, within EX31_RS15370 at 25.066 kb on + strand, within EX31_RS15370 at 25.066 kb on + strand, within EX31_RS15370 at 25.066 kb on + strand, within EX31_RS15370 at 25.066 kb on + strand, within EX31_RS15370 at 25.067 kb on - strand, within EX31_RS15370 at 25.249 kb on + strand, within EX31_RS15370 at 25.249 kb on + strand, within EX31_RS15370 at 25.249 kb on + strand, within EX31_RS15370 at 25.249 kb on + strand, within EX31_RS15370 at 25.250 kb on - strand, within EX31_RS15370 at 25.250 kb on - strand, within EX31_RS15370 at 25.250 kb on - strand, within EX31_RS15370 at 25.250 kb on - strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.253 kb on + strand, within EX31_RS15370 at 25.254 kb on - strand, within EX31_RS15370 at 25.294 kb on + strand, within EX31_RS15370 at 25.294 kb on + strand, within EX31_RS15370 at 25.295 kb on - strand, within EX31_RS15370 at 25.328 kb on + strand, within EX31_RS15370 at 25.329 kb on - strand, within EX31_RS15370 at 25.361 kb on + strand, within EX31_RS15370 at 25.373 kb on + strand, within EX31_RS15370 at 25.373 kb on + strand, within EX31_RS15370 at 25.373 kb on + strand, within EX31_RS15370 at 25.374 kb on - strand, within EX31_RS15370 at 25.450 kb on + strand, within EX31_RS15370 at 25.450 kb on + strand, within EX31_RS15370 at 25.450 kb on + strand, within EX31_RS15370 at 25.450 kb on + strand, within EX31_RS15370 at 25.451 kb on - strand, within EX31_RS15370 at 25.451 kb on - strand, within EX31_RS15370 at 25.472 kb on + strand, within EX31_RS15370 at 25.472 kb on + strand, within EX31_RS15370 at 25.499 kb on + strand, within EX31_RS15370 at 25.499 kb on + strand, within EX31_RS15370 at 25.500 kb on - strand, within EX31_RS15370 at 25.511 kb on + strand, within EX31_RS15370 at 25.522 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.552 kb on + strand, within EX31_RS15370 at 25.553 kb on - strand, within EX31_RS15370 at 25.553 kb on - strand, within EX31_RS15370 at 25.553 kb on - strand, within EX31_RS15370 at 25.553 kb on - strand, within EX31_RS15370 at 25.553 kb on - strand, within EX31_RS15370 at 25.721 kb on + strand, within EX31_RS15370 at 25.721 kb on + strand, within EX31_RS15370
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 22,747 + EX31_RS15355 0.77 +0.6 22,748 - EX31_RS15355 0.78 -0.2 22,799 + +0.2 22,799 + -0.7 22,799 + -0.8 22,799 + -0.3 22,799 + +0.1 22,799 + +2.2 22,800 - +0.6 22,800 - -1.4 22,835 - +0.9 22,850 + -0.2 23,198 + EX31_RS15360 0.43 +2.3 23,198 + EX31_RS15360 0.43 -0.8 23,198 + EX31_RS15360 0.43 -1.0 23,199 - EX31_RS15360 0.43 +0.3 23,199 - EX31_RS15360 0.43 +0.9 23,199 - EX31_RS15360 0.43 +0.2 23,201 + EX31_RS15360 0.43 -0.3 23,202 - EX31_RS15360 0.44 -1.2 23,245 + EX31_RS15360 0.49 +0.5 23,333 + EX31_RS15360 0.59 -0.4 23,333 + EX31_RS15360 0.59 +0.3 23,333 + EX31_RS15360 0.59 +0.9 23,334 - EX31_RS15360 0.60 -0.4 23,632 + +1.6 23,633 - -0.8 23,633 - -1.2 23,642 + +1.6 23,642 + +0.7 23,642 + +0.8 23,642 + +0.7 23,642 + +0.3 23,642 + +0.6 23,642 + +0.6 23,642 + +0.4 23,642 + +2.2 23,643 - +0.3 23,643 - +0.4 23,644 + +1.6 23,644 + +1.3 23,644 + -0.5 23,644 + -0.1 23,644 + +0.2 23,644 + -0.0 23,644 + -1.3 23,644 + -1.8 23,644 + +1.2 23,644 + +1.2 23,644 + -0.1 23,644 + -0.8 23,644 + +0.2 23,645 - -0.2 23,645 - +1.6 23,645 - -0.1 23,645 - +0.3 23,645 - +1.0 23,700 - -1.4 23,727 + -0.9 23,727 + +1.4 23,727 + +0.8 23,727 + +0.3 23,787 + -0.1 23,799 + -0.4 23,898 + EX31_RS15365 0.15 -0.2 23,898 + EX31_RS15365 0.15 -0.7 23,898 + EX31_RS15365 0.15 +0.2 23,899 - EX31_RS15365 0.15 -0.7 23,899 - EX31_RS15365 0.15 -0.1 23,899 - EX31_RS15365 0.15 +0.6 23,900 + EX31_RS15365 0.15 -2.3 23,901 - EX31_RS15365 0.15 -0.9 23,901 - EX31_RS15365 0.15 -0.1 23,913 - EX31_RS15365 0.16 -1.2 23,955 + EX31_RS15365 0.20 +0.5 23,955 + EX31_RS15365 0.20 +0.8 23,956 - EX31_RS15365 0.20 +0.2 23,993 + EX31_RS15365 0.23 +0.2 24,057 + EX31_RS15365 0.28 +1.1 24,057 + EX31_RS15365 0.28 -0.7 24,057 + EX31_RS15365 0.28 -1.4 24,057 + EX31_RS15365 0.28 -0.6 24,057 + EX31_RS15365 0.28 -1.0 24,057 + EX31_RS15365 0.28 -0.7 24,057 + EX31_RS15365 0.28 +1.7 24,058 - EX31_RS15365 0.28 +1.3 24,058 - EX31_RS15365 0.28 -1.4 24,058 - EX31_RS15365 0.28 -0.0 24,058 - EX31_RS15365 0.28 +0.0 24,072 - EX31_RS15365 0.30 -1.6 24,111 + EX31_RS15365 0.33 -1.6 24,111 + EX31_RS15365 0.33 -2.6 24,112 - EX31_RS15365 0.33 +0.4 24,112 - EX31_RS15365 0.33 +0.0 24,112 - EX31_RS15365 0.33 -0.2 24,112 - EX31_RS15365 0.33 +0.7 24,114 + EX31_RS15365 0.33 -1.2 24,115 - EX31_RS15365 0.33 -0.2 24,115 - EX31_RS15365 0.33 -0.4 24,115 - EX31_RS15365 0.33 +1.6 24,181 + EX31_RS15365 0.39 +0.6 24,181 + EX31_RS15365 0.39 -0.3 24,182 - EX31_RS15365 0.39 +0.6 24,182 - EX31_RS15365 0.39 +0.7 24,317 + EX31_RS15365 0.50 +1.0 24,317 + EX31_RS15365 0.50 +0.6 24,318 - EX31_RS15365 0.50 -0.2 24,351 + EX31_RS15365 0.53 +0.9 24,352 - EX31_RS15365 0.53 +1.0 24,352 - EX31_RS15365 0.53 +0.2 24,482 + EX31_RS15365 0.64 +0.3 24,482 + EX31_RS15365 0.64 +0.2 24,482 + EX31_RS15365 0.64 -0.7 24,482 + EX31_RS15365 0.64 +1.2 24,482 + EX31_RS15365 0.64 -0.8 24,482 + EX31_RS15365 0.64 -0.4 24,482 + EX31_RS15365 0.64 -1.7 24,483 - EX31_RS15365 0.64 +0.5 24,483 - EX31_RS15365 0.64 +0.5 24,483 - EX31_RS15365 0.64 +1.3 24,483 - EX31_RS15365 0.64 +0.0 24,483 - EX31_RS15365 0.64 +1.6 24,483 - EX31_RS15365 0.64 -0.8 24,593 + EX31_RS15365 0.73 +0.5 24,593 + EX31_RS15365 0.73 +0.6 24,593 + EX31_RS15365 0.73 -0.3 24,594 - EX31_RS15365 0.73 -0.8 24,594 - EX31_RS15365 0.73 +2.2 24,615 + EX31_RS15365 0.75 -0.5 24,615 + EX31_RS15365 0.75 +0.1 24,615 + EX31_RS15365 0.75 -0.2 24,615 + EX31_RS15365 0.75 -1.6 24,615 + EX31_RS15365 0.75 +1.8 24,615 + EX31_RS15365 0.75 -0.4 24,615 + EX31_RS15365 0.75 +0.3 24,615 + EX31_RS15365 0.75 +0.8 24,616 - EX31_RS15365 0.75 -0.7 24,616 - EX31_RS15365 0.75 -0.4 24,616 - EX31_RS15365 0.75 -1.4 24,616 - EX31_RS15365 0.75 +0.7 24,616 - EX31_RS15365 0.75 -1.9 24,617 + EX31_RS15365 0.75 +0.9 24,617 + EX31_RS15365 0.75 +0.0 24,618 - EX31_RS15365 0.75 -0.0 24,618 - EX31_RS15365 0.75 -0.0 24,647 + EX31_RS15365 0.77 +1.2 24,648 - EX31_RS15365 0.77 -0.3 24,659 + EX31_RS15365 0.78 +0.5 24,731 + EX31_RS15365 0.84 +0.2 24,731 + EX31_RS15365 0.84 +1.4 24,731 + EX31_RS15365 0.84 -0.2 24,731 + EX31_RS15365 0.84 +0.9 24,731 + EX31_RS15365 0.84 -0.4 24,731 + EX31_RS15365 0.84 +0.7 24,738 + EX31_RS15365 0.85 +0.6 24,738 + EX31_RS15365 0.85 -0.3 24,739 - EX31_RS15365 0.85 -0.1 24,739 - EX31_RS15365 0.85 +0.0 24,739 - EX31_RS15365 0.85 +2.2 24,739 - EX31_RS15365 0.85 -0.2 24,740 + EX31_RS15365 0.85 +1.4 24,740 + EX31_RS15365 0.85 +1.4 24,740 + EX31_RS15365 0.85 -0.4 24,740 + EX31_RS15365 0.85 +1.7 24,741 - EX31_RS15365 0.85 -0.2 24,783 - EX31_RS15365 0.89 +1.7 24,859 - +1.2 24,912 + +0.4 24,913 - +0.9 24,913 - -0.4 24,913 - -1.0 25,066 + EX31_RS15370 0.11 -0.4 25,066 + EX31_RS15370 0.11 -0.2 25,066 + EX31_RS15370 0.11 +0.3 25,066 + EX31_RS15370 0.11 +0.5 25,066 + EX31_RS15370 0.11 -0.6 25,067 - EX31_RS15370 0.11 -0.2 25,249 + EX31_RS15370 0.26 -0.4 25,249 + EX31_RS15370 0.26 -1.0 25,249 + EX31_RS15370 0.26 -0.6 25,249 + EX31_RS15370 0.26 +0.8 25,250 - EX31_RS15370 0.26 +0.9 25,250 - EX31_RS15370 0.26 +0.6 25,250 - EX31_RS15370 0.26 +1.7 25,250 - EX31_RS15370 0.26 -0.5 25,253 + EX31_RS15370 0.26 +0.6 25,253 + EX31_RS15370 0.26 -1.5 25,253 + EX31_RS15370 0.26 -1.2 25,253 + EX31_RS15370 0.26 -1.0 25,253 + EX31_RS15370 0.26 -0.8 25,253 + EX31_RS15370 0.26 -0.6 25,253 + EX31_RS15370 0.26 +1.2 25,254 - EX31_RS15370 0.26 +1.7 25,294 + EX31_RS15370 0.30 +2.2 25,294 + EX31_RS15370 0.30 +0.5 25,295 - EX31_RS15370 0.30 +0.5 25,328 + EX31_RS15370 0.33 +0.5 25,329 - EX31_RS15370 0.33 -0.3 25,361 + EX31_RS15370 0.35 +0.6 25,373 + EX31_RS15370 0.36 -0.1 25,373 + EX31_RS15370 0.36 -2.7 25,373 + EX31_RS15370 0.36 -0.3 25,374 - EX31_RS15370 0.36 +0.3 25,450 + EX31_RS15370 0.43 +0.4 25,450 + EX31_RS15370 0.43 +0.4 25,450 + EX31_RS15370 0.43 -1.3 25,450 + EX31_RS15370 0.43 -0.9 25,451 - EX31_RS15370 0.43 -0.4 25,451 - EX31_RS15370 0.43 -1.3 25,472 + EX31_RS15370 0.45 +1.4 25,472 + EX31_RS15370 0.45 +0.2 25,499 + EX31_RS15370 0.47 -0.0 25,499 + EX31_RS15370 0.47 +0.4 25,500 - EX31_RS15370 0.47 -1.0 25,511 + EX31_RS15370 0.48 +0.4 25,522 + EX31_RS15370 0.49 -0.4 25,552 + EX31_RS15370 0.51 +1.2 25,552 + EX31_RS15370 0.51 +0.2 25,552 + EX31_RS15370 0.51 -2.1 25,552 + EX31_RS15370 0.51 -1.3 25,552 + EX31_RS15370 0.51 +0.6 25,552 + EX31_RS15370 0.51 +0.5 25,552 + EX31_RS15370 0.51 +0.3 25,552 + EX31_RS15370 0.51 +0.4 25,552 + EX31_RS15370 0.51 -1.5 25,553 - EX31_RS15370 0.51 +1.2 25,553 - EX31_RS15370 0.51 +0.4 25,553 - EX31_RS15370 0.51 +0.6 25,553 - EX31_RS15370 0.51 -0.1 25,553 - EX31_RS15370 0.51 -0.2 25,721 + EX31_RS15370 0.65 -0.2 25,721 + EX31_RS15370 0.65 -1.8
Or see this region's nucleotide sequence