Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13765 and EX31_RS13770 are separated by 397 nucleotides EX31_RS13770 and EX31_RS13775 are separated by 113 nucleotides EX31_RS13775 and EX31_RS13780 are separated by 194 nucleotides EX31_RS13780 and EX31_RS13785 are separated by 1 nucleotides
EX31_RS13765: EX31_RS13765 - anhydro-N-acetylmuramic acid kinase, at 12,408 to 13,526
_RS13765
EX31_RS13770: EX31_RS13770 - glycine zipper 2TM domain-containing protein, at 13,924 to 14,394
_RS13770
EX31_RS13775: EX31_RS13775 - transcriptional regulator SlyA, at 14,508 to 14,942
_RS13775
EX31_RS13780: EX31_RS13780 - DUF1656 domain-containing protein, at 15,137 to 15,361
_RS13780
EX31_RS13785: EX31_RS13785 - HlyD family secretion protein, at 15,363 to 16,220
_RS13785
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.950 kb on + strand, within EX31_RS13765 at 12.950 kb on + strand, within EX31_RS13765 at 12.950 kb on + strand, within EX31_RS13765 at 12.950 kb on + strand, within EX31_RS13765 at 12.951 kb on - strand, within EX31_RS13765 at 13.038 kb on - strand, within EX31_RS13765 at 13.175 kb on + strand, within EX31_RS13765 at 13.190 kb on + strand, within EX31_RS13765 at 13.191 kb on - strand, within EX31_RS13765 at 13.271 kb on + strand, within EX31_RS13765 at 13.272 kb on - strand, within EX31_RS13765 at 13.293 kb on + strand, within EX31_RS13765 at 13.293 kb on + strand, within EX31_RS13765 at 13.293 kb on + strand, within EX31_RS13765 at 13.294 kb on - strand, within EX31_RS13765 at 13.294 kb on - strand, within EX31_RS13765 at 13.294 kb on - strand, within EX31_RS13765 at 13.294 kb on - strand, within EX31_RS13765 at 13.294 kb on - strand, within EX31_RS13765 at 13.317 kb on - strand, within EX31_RS13765 at 13.317 kb on - strand, within EX31_RS13765 at 13.410 kb on - strand, within EX31_RS13765 at 13.420 kb on + strand at 13.420 kb on + strand at 13.421 kb on - strand at 13.464 kb on + strand at 13.510 kb on + strand at 13.511 kb on - strand at 13.511 kb on - strand at 13.511 kb on - strand at 13.511 kb on - strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.530 kb on + strand at 13.530 kb on + strand at 13.530 kb on + strand at 13.530 kb on + strand at 13.530 kb on + strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.531 kb on - strand at 13.539 kb on + strand at 13.540 kb on - strand at 13.540 kb on - strand at 13.540 kb on - strand at 13.569 kb on - strand at 13.583 kb on - strand at 13.583 kb on - strand at 13.583 kb on - strand at 13.590 kb on - strand at 13.627 kb on + strand at 13.628 kb on - strand at 13.704 kb on + strand at 13.704 kb on + strand at 13.724 kb on - strand at 13.749 kb on + strand at 13.768 kb on + strand at 13.769 kb on - strand at 13.771 kb on + strand at 13.771 kb on + strand at 13.771 kb on + strand at 13.774 kb on + strand at 13.774 kb on + strand at 13.774 kb on + strand at 13.775 kb on - strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.793 kb on + strand at 13.794 kb on - strand at 13.794 kb on - strand at 13.795 kb on + strand at 13.795 kb on + strand at 13.796 kb on - strand at 13.796 kb on - strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.798 kb on + strand at 13.799 kb on - strand at 13.799 kb on - strand at 13.799 kb on - strand at 13.799 kb on - strand at 13.799 kb on - strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.817 kb on + strand at 13.818 kb on - strand at 13.818 kb on - strand at 13.818 kb on - strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.822 kb on + strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.823 kb on - strand at 13.824 kb on + strand at 13.824 kb on + strand at 13.824 kb on + strand at 13.824 kb on + strand at 13.824 kb on + strand at 13.824 kb on + strand at 13.825 kb on - strand at 13.825 kb on - strand at 13.825 kb on - strand at 13.825 kb on - strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.896 kb on - strand at 13.907 kb on + strand at 13.907 kb on + strand at 13.907 kb on + strand at 13.907 kb on + strand at 13.907 kb on + strand at 13.907 kb on + strand at 13.908 kb on - strand at 13.908 kb on - strand at 13.908 kb on - strand at 13.908 kb on - strand at 13.910 kb on + strand at 13.910 kb on + strand at 13.910 kb on + strand at 13.910 kb on + strand at 13.910 kb on + strand at 13.919 kb on + strand at 13.919 kb on + strand at 13.919 kb on + strand at 13.919 kb on + strand at 13.920 kb on - strand at 13.920 kb on - strand at 13.923 kb on + strand at 13.924 kb on - strand at 13.924 kb on - strand at 13.929 kb on + strand at 13.929 kb on + strand at 13.929 kb on + strand at 13.930 kb on - strand at 13.987 kb on - strand, within EX31_RS13770 at 14.008 kb on + strand, within EX31_RS13770 at 14.008 kb on + strand, within EX31_RS13770 at 14.008 kb on + strand, within EX31_RS13770 at 14.008 kb on + strand, within EX31_RS13770 at 14.008 kb on + strand, within EX31_RS13770 at 14.047 kb on + strand, within EX31_RS13770 at 14.047 kb on + strand, within EX31_RS13770 at 14.075 kb on + strand, within EX31_RS13770 at 14.175 kb on + strand, within EX31_RS13770 at 14.175 kb on + strand, within EX31_RS13770 at 14.258 kb on + strand, within EX31_RS13770 at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.373 kb on + strand at 14.374 kb on - strand at 14.453 kb on - strand at 14.453 kb on - strand at 14.453 kb on - strand at 14.495 kb on + strand at 14.495 kb on + strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.625 kb on - strand, within EX31_RS13775 at 14.625 kb on - strand, within EX31_RS13775 at 14.748 kb on - strand, within EX31_RS13775 at 14.820 kb on - strand, within EX31_RS13775 at 14.820 kb on - strand, within EX31_RS13775 at 14.908 kb on - strand at 14.962 kb on - strand at 14.970 kb on - strand at 14.995 kb on - strand at 15.010 kb on - strand at 15.022 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.030 kb on - strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.034 kb on - strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on + strand at 15.035 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.036 kb on - strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.038 kb on + strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.039 kb on - strand at 15.041 kb on + strand at 15.041 kb on + strand at 15.041 kb on + strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.042 kb on - strand at 15.084 kb on + strand at 15.085 kb on - strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.095 kb on - strand at 15.096 kb on + strand at 15.122 kb on - strand at 15.216 kb on + strand, within EX31_RS13780 at 15.270 kb on - strand, within EX31_RS13780 at 15.307 kb on - strand, within EX31_RS13780 at 15.307 kb on - strand, within EX31_RS13780 at 15.325 kb on + strand, within EX31_RS13780 at 15.326 kb on - strand, within EX31_RS13780 at 15.346 kb on + strand at 15.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 12,950 + EX31_RS13765 0.48 -1.1 12,950 + EX31_RS13765 0.48 +1.5 12,950 + EX31_RS13765 0.48 +0.8 12,950 + EX31_RS13765 0.48 -0.4 12,951 - EX31_RS13765 0.49 +0.1 13,038 - EX31_RS13765 0.56 +1.1 13,175 + EX31_RS13765 0.69 +1.2 13,190 + EX31_RS13765 0.70 +1.2 13,191 - EX31_RS13765 0.70 +0.9 13,271 + EX31_RS13765 0.77 +1.7 13,272 - EX31_RS13765 0.77 +0.4 13,293 + EX31_RS13765 0.79 -0.6 13,293 + EX31_RS13765 0.79 +0.1 13,293 + EX31_RS13765 0.79 -0.7 13,294 - EX31_RS13765 0.79 -1.4 13,294 - EX31_RS13765 0.79 +1.3 13,294 - EX31_RS13765 0.79 +1.1 13,294 - EX31_RS13765 0.79 -0.1 13,294 - EX31_RS13765 0.79 +0.5 13,317 - EX31_RS13765 0.81 +0.1 13,317 - EX31_RS13765 0.81 +2.1 13,410 - EX31_RS13765 0.90 +0.6 13,420 + -2.2 13,420 + +0.5 13,421 - +2.7 13,464 + +1.4 13,510 + -0.4 13,511 - -0.5 13,511 - -0.9 13,511 - -0.1 13,511 - +0.8 13,526 + +1.1 13,526 + -1.2 13,527 - +0.1 13,527 - -0.1 13,530 + +0.5 13,530 + +2.1 13,530 + +2.1 13,530 + -1.1 13,530 + +1.1 13,531 - +0.2 13,531 - -0.1 13,531 - +0.2 13,531 - -0.6 13,531 - -0.5 13,531 - -0.9 13,531 - -0.7 13,539 + +1.1 13,540 - +0.3 13,540 - +0.3 13,540 - -0.2 13,569 - +1.5 13,583 - +0.1 13,583 - +0.2 13,583 - -0.5 13,590 - -0.5 13,627 + +0.5 13,628 - +0.9 13,704 + +1.3 13,704 + +2.7 13,724 - +0.3 13,749 + +0.6 13,768 + +0.1 13,769 - -0.1 13,771 + -0.8 13,771 + -0.6 13,771 + -0.4 13,774 + +2.1 13,774 + -1.1 13,774 + +0.3 13,775 - +0.4 13,793 + -0.7 13,793 + +0.2 13,793 + +0.2 13,793 + -0.5 13,793 + +0.5 13,793 + -0.7 13,793 + +0.5 13,793 + +0.1 13,793 + -0.6 13,794 - -0.4 13,794 - -0.8 13,795 + -1.1 13,795 + -0.0 13,796 - -1.3 13,796 - +0.7 13,798 + -0.7 13,798 + -0.4 13,798 + +1.4 13,798 + -2.5 13,798 + -0.6 13,798 + -0.2 13,798 + -0.1 13,798 + -1.3 13,798 + -0.1 13,798 + -0.1 13,799 - +0.6 13,799 - -1.6 13,799 - +1.1 13,799 - -1.6 13,799 - +0.5 13,817 + -0.5 13,817 + +1.5 13,817 + -0.8 13,817 + -0.4 13,817 + +1.1 13,817 + +0.1 13,817 + +0.9 13,817 + -0.2 13,817 + +0.8 13,817 + +0.1 13,817 + -1.3 13,817 + +1.7 13,817 + -2.2 13,817 + +0.6 13,817 + -1.0 13,817 + -0.2 13,818 - +0.8 13,818 - -1.4 13,818 - -0.3 13,822 + -1.1 13,822 + -0.4 13,822 + +0.1 13,822 + -0.1 13,822 + +0.7 13,822 + -1.8 13,822 + -1.0 13,822 + +0.1 13,822 + -0.3 13,822 + +0.6 13,822 + -0.9 13,822 + -0.8 13,822 + -1.0 13,822 + +0.2 13,822 + -0.2 13,822 + +2.1 13,822 + -0.4 13,823 - +0.2 13,823 - +1.1 13,823 - +0.8 13,823 - +0.8 13,823 - +1.1 13,823 - +0.5 13,823 - +0.7 13,824 + -0.2 13,824 + -0.8 13,824 + -0.9 13,824 + -1.3 13,824 + -0.9 13,824 + -0.6 13,825 - +0.1 13,825 - +0.7 13,825 - -0.6 13,825 - -0.7 13,895 + -0.2 13,895 + +1.1 13,895 + -0.6 13,895 + +0.1 13,895 + +0.3 13,895 + +0.7 13,896 - +0.1 13,907 + -0.3 13,907 + -1.2 13,907 + -0.1 13,907 + -0.2 13,907 + -0.5 13,907 + -1.1 13,908 - +2.1 13,908 - +0.7 13,908 - -0.3 13,908 - -0.9 13,910 + +0.2 13,910 + -0.7 13,910 + -1.8 13,910 + -1.0 13,910 + +0.0 13,919 + +0.4 13,919 + +0.3 13,919 + +0.1 13,919 + -0.7 13,920 - +0.1 13,920 - +1.6 13,923 + -1.6 13,924 - +0.4 13,924 - -1.1 13,929 + +0.1 13,929 + +0.9 13,929 + +0.1 13,930 - +1.7 13,987 - EX31_RS13770 0.13 -0.7 14,008 + EX31_RS13770 0.18 -0.9 14,008 + EX31_RS13770 0.18 +0.3 14,008 + EX31_RS13770 0.18 -1.2 14,008 + EX31_RS13770 0.18 -1.2 14,008 + EX31_RS13770 0.18 -0.4 14,047 + EX31_RS13770 0.26 +0.1 14,047 + EX31_RS13770 0.26 -0.0 14,075 + EX31_RS13770 0.32 -0.4 14,175 + EX31_RS13770 0.53 -1.5 14,175 + EX31_RS13770 0.53 -0.1 14,258 + EX31_RS13770 0.71 -1.5 14,373 + -0.9 14,373 + -0.2 14,373 + -1.2 14,373 + -0.0 14,373 + +1.1 14,373 + +0.3 14,373 + -0.4 14,374 - +2.1 14,453 - +0.8 14,453 - -0.9 14,453 - -1.0 14,495 + +1.6 14,495 + -0.5 14,496 - -0.0 14,496 - +1.0 14,496 - -0.2 14,625 - EX31_RS13775 0.27 -0.5 14,625 - EX31_RS13775 0.27 -0.2 14,748 - EX31_RS13775 0.55 +0.5 14,820 - EX31_RS13775 0.72 -0.0 14,820 - EX31_RS13775 0.72 -0.2 14,908 - +0.8 14,962 - -0.7 14,970 - -1.2 14,995 - -0.5 15,010 - +0.5 15,022 - -0.4 15,029 + +0.5 15,030 - +0.9 15,030 - +0.6 15,030 - -0.0 15,030 - -0.6 15,030 - -0.2 15,030 - -0.8 15,031 + +0.9 15,032 - -0.2 15,032 - -0.1 15,033 + +0.4 15,033 + -0.5 15,033 + -0.4 15,033 + -0.2 15,033 + -0.1 15,033 + +0.0 15,034 - -0.9 15,034 - +1.2 15,034 - -0.2 15,034 - -1.0 15,034 - -0.5 15,034 - +0.9 15,034 - +0.5 15,034 - -0.9 15,034 - -1.2 15,034 - +0.5 15,034 - -0.3 15,035 + +0.8 15,035 + -2.7 15,035 + -0.4 15,035 + +0.2 15,035 + -0.6 15,035 + +0.8 15,035 + -1.7 15,035 + -0.5 15,035 - +0.4 15,036 - -0.7 15,036 - -0.2 15,036 - -1.2 15,036 - +0.5 15,036 - -0.7 15,036 - -0.4 15,036 - +0.7 15,036 - -1.2 15,036 - +0.2 15,036 - -0.4 15,036 - +1.1 15,038 + +0.7 15,038 + -0.0 15,038 + +0.9 15,038 + +0.5 15,038 + +0.3 15,039 - +0.9 15,039 - +1.0 15,039 - +0.7 15,039 - +1.7 15,039 - -0.1 15,039 - +0.5 15,039 - -0.8 15,039 - +0.7 15,039 - -0.2 15,039 - -1.3 15,039 - +1.2 15,041 + +0.1 15,041 + +1.4 15,041 + +0.1 15,042 - +0.8 15,042 - +1.4 15,042 - -0.4 15,042 - -0.2 15,042 - -0.7 15,042 - -0.3 15,084 + +0.5 15,085 - +0.6 15,094 + +0.9 15,094 + +1.1 15,095 - +0.1 15,095 - -1.6 15,095 - -0.0 15,095 - -1.0 15,095 - +0.0 15,096 + +1.0 15,122 - -0.9 15,216 + EX31_RS13780 0.35 -0.3 15,270 - EX31_RS13780 0.59 +1.6 15,307 - EX31_RS13780 0.76 +0.5 15,307 - EX31_RS13780 0.76 -0.9 15,325 + EX31_RS13780 0.84 +1.3 15,326 - EX31_RS13780 0.84 +1.1 15,346 + +1.1 15,393 - -1.2
Or see this region's nucleotide sequence