Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02290 and EX31_RS02295 are separated by 488 nucleotides EX31_RS02295 and EX31_RS02300 are separated by 45 nucleotides
EX31_RS02290: EX31_RS02290 - LacI family DNA-binding transcriptional regulator, at 494,378 to 495,412
_RS02290
EX31_RS02295: EX31_RS02295 - gluconate 2-dehydrogenase subunit 3 family protein, at 495,901 to 496,626
_RS02295
EX31_RS02300: EX31_RS02300 - GMC family oxidoreductase, at 496,672 to 498,441
_RS02300
Position (kb) 495
496
497 Strain fitness (log2 ratio) -1
0
1
2
3 at 494.971 kb on + strand, within EX31_RS02290 at 494.971 kb on + strand, within EX31_RS02290 at 494.972 kb on - strand, within EX31_RS02290 at 495.053 kb on - strand, within EX31_RS02290 at 495.082 kb on + strand, within EX31_RS02290 at 495.204 kb on + strand, within EX31_RS02290 at 495.325 kb on + strand at 495.326 kb on - strand at 495.326 kb on - strand at 495.326 kb on - strand at 495.367 kb on + strand at 495.367 kb on + strand at 495.367 kb on + strand at 495.368 kb on - strand at 495.368 kb on - strand at 495.383 kb on + strand at 495.394 kb on + strand at 495.411 kb on - strand at 495.448 kb on + strand at 495.448 kb on + strand at 495.449 kb on - strand at 495.449 kb on - strand at 495.498 kb on + strand at 495.499 kb on - strand at 495.510 kb on - strand at 495.526 kb on + strand at 495.527 kb on - strand at 495.527 kb on - strand at 495.658 kb on - strand at 495.782 kb on + strand at 495.783 kb on - strand at 495.783 kb on - strand at 495.856 kb on + strand at 495.856 kb on + strand at 495.857 kb on - strand at 495.900 kb on + strand at 496.028 kb on + strand, within EX31_RS02295 at 496.047 kb on - strand, within EX31_RS02295 at 496.074 kb on + strand, within EX31_RS02295 at 496.198 kb on + strand, within EX31_RS02295 at 496.198 kb on + strand, within EX31_RS02295 at 496.199 kb on - strand, within EX31_RS02295 at 496.200 kb on + strand, within EX31_RS02295 at 496.201 kb on - strand, within EX31_RS02295 at 496.489 kb on + strand, within EX31_RS02295 at 496.641 kb on + strand at 496.667 kb on + strand at 496.668 kb on - strand at 496.682 kb on + strand at 496.682 kb on + strand at 496.841 kb on - strand at 496.841 kb on - strand at 496.847 kb on - strand at 496.860 kb on + strand, within EX31_RS02300 at 496.860 kb on + strand, within EX31_RS02300 at 496.903 kb on + strand, within EX31_RS02300 at 496.903 kb on + strand, within EX31_RS02300 at 496.903 kb on + strand, within EX31_RS02300 at 496.904 kb on - strand, within EX31_RS02300 at 497.054 kb on - strand, within EX31_RS02300 at 497.128 kb on + strand, within EX31_RS02300 at 497.128 kb on + strand, within EX31_RS02300 at 497.129 kb on - strand, within EX31_RS02300 at 497.133 kb on + strand, within EX31_RS02300 at 497.145 kb on + strand, within EX31_RS02300 at 497.146 kb on - strand, within EX31_RS02300 at 497.379 kb on + strand, within EX31_RS02300 at 497.380 kb on - strand, within EX31_RS02300 at 497.380 kb on - strand, within EX31_RS02300 at 497.400 kb on + strand, within EX31_RS02300 at 497.472 kb on + strand, within EX31_RS02300 at 497.482 kb on + strand, within EX31_RS02300 at 497.483 kb on - strand, within EX31_RS02300 at 497.551 kb on + strand, within EX31_RS02300 at 497.551 kb on + strand, within EX31_RS02300 at 497.551 kb on + strand, within EX31_RS02300 at 497.551 kb on + strand, within EX31_RS02300 at 497.551 kb on + strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300 at 497.552 kb on - strand, within EX31_RS02300
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 494,971 + EX31_RS02290 0.57 +0.2 494,971 + EX31_RS02290 0.57 -1.2 494,972 - EX31_RS02290 0.57 -1.0 495,053 - EX31_RS02290 0.65 -0.8 495,082 + EX31_RS02290 0.68 +0.8 495,204 + EX31_RS02290 0.80 -0.1 495,325 + -0.3 495,326 - -0.3 495,326 - -1.2 495,326 - +3.1 495,367 + -0.2 495,367 + +1.7 495,367 + -0.4 495,368 - +0.6 495,368 - +0.8 495,383 + +0.8 495,394 + -1.0 495,411 - -0.5 495,448 + -0.8 495,448 + +0.5 495,449 - -1.1 495,449 - +0.9 495,498 + +0.8 495,499 - +1.2 495,510 - +1.1 495,526 + +0.1 495,527 - -1.5 495,527 - -0.7 495,658 - +0.0 495,782 + +0.2 495,783 - +0.5 495,783 - +1.8 495,856 + +0.3 495,856 + -0.2 495,857 - -0.4 495,900 + +0.2 496,028 + EX31_RS02295 0.17 +0.7 496,047 - EX31_RS02295 0.20 +0.3 496,074 + EX31_RS02295 0.24 +0.5 496,198 + EX31_RS02295 0.41 -0.5 496,198 + EX31_RS02295 0.41 -0.5 496,199 - EX31_RS02295 0.41 +1.2 496,200 + EX31_RS02295 0.41 -0.3 496,201 - EX31_RS02295 0.41 +1.9 496,489 + EX31_RS02295 0.81 +1.4 496,641 + -0.2 496,667 + -1.5 496,668 - +1.0 496,682 + +1.5 496,682 + +0.1 496,841 - +0.6 496,841 - -1.2 496,847 - -0.5 496,860 + EX31_RS02300 0.11 +0.3 496,860 + EX31_RS02300 0.11 -1.4 496,903 + EX31_RS02300 0.13 -0.5 496,903 + EX31_RS02300 0.13 -1.2 496,903 + EX31_RS02300 0.13 +0.1 496,904 - EX31_RS02300 0.13 +0.0 497,054 - EX31_RS02300 0.22 -0.2 497,128 + EX31_RS02300 0.26 +1.1 497,128 + EX31_RS02300 0.26 +2.1 497,129 - EX31_RS02300 0.26 +0.3 497,133 + EX31_RS02300 0.26 -0.1 497,145 + EX31_RS02300 0.27 +0.1 497,146 - EX31_RS02300 0.27 +0.5 497,379 + EX31_RS02300 0.40 -0.6 497,380 - EX31_RS02300 0.40 +1.2 497,380 - EX31_RS02300 0.40 +2.1 497,400 + EX31_RS02300 0.41 +0.1 497,472 + EX31_RS02300 0.45 +0.5 497,482 + EX31_RS02300 0.46 -0.5 497,483 - EX31_RS02300 0.46 -0.6 497,551 + EX31_RS02300 0.50 +0.7 497,551 + EX31_RS02300 0.50 -0.2 497,551 + EX31_RS02300 0.50 +0.8 497,551 + EX31_RS02300 0.50 +1.2 497,551 + EX31_RS02300 0.50 +1.4 497,552 - EX31_RS02300 0.50 +1.4 497,552 - EX31_RS02300 0.50 -0.8 497,552 - EX31_RS02300 0.50 +0.5 497,552 - EX31_RS02300 0.50 -0.0 497,552 - EX31_RS02300 0.50 +1.7 497,552 - EX31_RS02300 0.50 +1.0
Or see this region's nucleotide sequence