Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00160 and EX31_RS00165 are separated by 125 nucleotides EX31_RS00165 and EX31_RS00170 are separated by 213 nucleotides EX31_RS00170 and EX31_RS25880 are separated by 143 nucleotides EX31_RS25880 and EX31_RS00175 are separated by 278 nucleotides
EX31_RS00160: EX31_RS00160 - trans-2-enoyl-CoA reductase family protein, at 33,899 to 35,095
_RS00160
EX31_RS00165: EX31_RS00165 - zinc-ribbon domain and TM2 domain-containing protein, at 35,221 to 35,523
_RS00165
EX31_RS00170: EX31_RS00170 - DUF1471 domain-containing protein, at 35,737 to 36,042
_RS00170
EX31_RS25880: EX31_RS25880 - hypothetical protein, at 36,186 to 36,407
_RS25880
EX31_RS00175: EX31_RS00175 - membrane protein, at 36,686 to 37,963
_RS00175
Position (kb) 35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.093 kb on + strand at 35.093 kb on + strand at 35.094 kb on - strand at 35.094 kb on - strand at 35.103 kb on + strand at 35.108 kb on + strand at 35.170 kb on - strand at 35.202 kb on + strand at 35.203 kb on - strand at 35.205 kb on - strand at 35.213 kb on - strand at 35.216 kb on + strand at 35.264 kb on + strand, within EX31_RS00165 at 35.264 kb on + strand, within EX31_RS00165 at 35.265 kb on - strand, within EX31_RS00165 at 35.265 kb on - strand, within EX31_RS00165 at 35.265 kb on - strand, within EX31_RS00165 at 35.268 kb on + strand, within EX31_RS00165 at 35.269 kb on - strand, within EX31_RS00165 at 35.269 kb on - strand, within EX31_RS00165 at 35.269 kb on - strand, within EX31_RS00165 at 35.301 kb on + strand, within EX31_RS00165 at 35.302 kb on - strand, within EX31_RS00165 at 35.302 kb on - strand, within EX31_RS00165 at 35.324 kb on + strand, within EX31_RS00165 at 35.324 kb on + strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.325 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.327 kb on - strand, within EX31_RS00165 at 35.355 kb on - strand, within EX31_RS00165 at 35.355 kb on - strand, within EX31_RS00165 at 35.355 kb on - strand, within EX31_RS00165 at 35.355 kb on - strand, within EX31_RS00165 at 35.362 kb on - strand, within EX31_RS00165 at 35.362 kb on - strand, within EX31_RS00165 at 35.392 kb on + strand, within EX31_RS00165 at 35.393 kb on - strand, within EX31_RS00165 at 35.393 kb on - strand, within EX31_RS00165 at 35.393 kb on - strand, within EX31_RS00165 at 35.419 kb on - strand, within EX31_RS00165 at 35.436 kb on + strand, within EX31_RS00165 at 35.436 kb on + strand, within EX31_RS00165 at 35.437 kb on - strand, within EX31_RS00165 at 35.437 kb on - strand, within EX31_RS00165 at 35.437 kb on - strand, within EX31_RS00165 at 35.437 kb on - strand, within EX31_RS00165 at 35.470 kb on - strand, within EX31_RS00165 at 35.515 kb on - strand at 35.515 kb on - strand at 35.528 kb on - strand at 35.535 kb on + strand at 35.535 kb on + strand at 35.536 kb on - strand at 35.536 kb on - strand at 35.536 kb on - strand at 35.536 kb on - strand at 35.536 kb on - strand at 35.571 kb on - strand at 35.576 kb on + strand at 35.577 kb on - strand at 35.658 kb on + strand at 35.712 kb on - strand at 35.712 kb on - strand at 35.744 kb on + strand at 35.769 kb on - strand, within EX31_RS00170 at 35.769 kb on - strand, within EX31_RS00170 at 35.769 kb on - strand, within EX31_RS00170 at 35.769 kb on - strand, within EX31_RS00170 at 36.017 kb on - strand at 36.126 kb on + strand at 36.127 kb on - strand at 36.165 kb on - strand at 36.219 kb on + strand, within EX31_RS25880 at 36.266 kb on - strand, within EX31_RS25880 at 36.266 kb on - strand, within EX31_RS25880 at 36.266 kb on - strand, within EX31_RS25880 at 36.266 kb on - strand, within EX31_RS25880 at 36.266 kb on - strand, within EX31_RS25880 at 36.315 kb on + strand, within EX31_RS25880 at 36.315 kb on + strand, within EX31_RS25880 at 36.372 kb on - strand, within EX31_RS25880 at 36.372 kb on - strand, within EX31_RS25880 at 36.372 kb on - strand, within EX31_RS25880 at 36.372 kb on - strand, within EX31_RS25880 at 36.372 kb on - strand, within EX31_RS25880 at 36.437 kb on + strand at 36.437 kb on + strand at 36.437 kb on + strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.438 kb on - strand at 36.459 kb on - strand at 36.481 kb on - strand at 36.481 kb on - strand at 36.481 kb on - strand at 36.662 kb on - strand at 36.663 kb on + strand at 36.724 kb on + strand at 36.757 kb on - strand at 36.757 kb on - strand at 36.774 kb on + strand at 36.818 kb on - strand, within EX31_RS00175 at 36.892 kb on + strand, within EX31_RS00175 at 36.986 kb on - strand, within EX31_RS00175 at 36.995 kb on - strand, within EX31_RS00175 at 36.995 kb on - strand, within EX31_RS00175 at 36.997 kb on + strand, within EX31_RS00175 at 37.030 kb on + strand, within EX31_RS00175
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 35,093 + +0.0 35,093 + -0.2 35,094 - -0.0 35,094 - -0.9 35,103 + +0.5 35,108 + -0.1 35,170 - +0.7 35,202 + +0.5 35,203 - -0.9 35,205 - -1.0 35,213 - +1.1 35,216 + -0.9 35,264 + EX31_RS00165 0.14 +1.4 35,264 + EX31_RS00165 0.14 +0.1 35,265 - EX31_RS00165 0.15 +0.2 35,265 - EX31_RS00165 0.15 +1.2 35,265 - EX31_RS00165 0.15 +0.8 35,268 + EX31_RS00165 0.16 +0.1 35,269 - EX31_RS00165 0.16 +0.4 35,269 - EX31_RS00165 0.16 +0.0 35,269 - EX31_RS00165 0.16 -0.8 35,301 + EX31_RS00165 0.26 +0.4 35,302 - EX31_RS00165 0.27 +0.5 35,302 - EX31_RS00165 0.27 -0.6 35,324 + EX31_RS00165 0.34 +0.1 35,324 + EX31_RS00165 0.34 +1.9 35,325 - EX31_RS00165 0.34 +0.2 35,325 - EX31_RS00165 0.34 +2.5 35,325 - EX31_RS00165 0.34 +0.3 35,325 - EX31_RS00165 0.34 -0.3 35,325 - EX31_RS00165 0.34 -0.2 35,325 - EX31_RS00165 0.34 +2.1 35,325 - EX31_RS00165 0.34 +0.6 35,325 - EX31_RS00165 0.34 -0.6 35,325 - EX31_RS00165 0.34 -0.8 35,327 - EX31_RS00165 0.35 +1.1 35,327 - EX31_RS00165 0.35 +0.3 35,327 - EX31_RS00165 0.35 +1.3 35,327 - EX31_RS00165 0.35 +0.3 35,327 - EX31_RS00165 0.35 +0.8 35,327 - EX31_RS00165 0.35 -0.5 35,327 - EX31_RS00165 0.35 -0.5 35,327 - EX31_RS00165 0.35 -1.0 35,355 - EX31_RS00165 0.44 +1.1 35,355 - EX31_RS00165 0.44 -0.8 35,355 - EX31_RS00165 0.44 +0.3 35,355 - EX31_RS00165 0.44 -1.0 35,362 - EX31_RS00165 0.47 +1.2 35,362 - EX31_RS00165 0.47 +0.7 35,392 + EX31_RS00165 0.56 -1.2 35,393 - EX31_RS00165 0.57 -1.6 35,393 - EX31_RS00165 0.57 +0.2 35,393 - EX31_RS00165 0.57 +0.9 35,419 - EX31_RS00165 0.65 -0.4 35,436 + EX31_RS00165 0.71 +1.2 35,436 + EX31_RS00165 0.71 -0.2 35,437 - EX31_RS00165 0.71 +0.3 35,437 - EX31_RS00165 0.71 -0.2 35,437 - EX31_RS00165 0.71 +0.8 35,437 - EX31_RS00165 0.71 +0.5 35,470 - EX31_RS00165 0.82 +0.4 35,515 - -0.1 35,515 - -0.9 35,528 - -0.5 35,535 + +0.0 35,535 + -0.2 35,536 - -2.2 35,536 - +1.5 35,536 - +0.7 35,536 - -0.1 35,536 - -0.7 35,571 - -0.5 35,576 + +1.0 35,577 - -1.6 35,658 + +1.5 35,712 - +0.5 35,712 - +0.4 35,744 + +0.6 35,769 - EX31_RS00170 0.10 -0.5 35,769 - EX31_RS00170 0.10 -1.3 35,769 - EX31_RS00170 0.10 -1.1 35,769 - EX31_RS00170 0.10 +0.4 36,017 - -1.2 36,126 + +0.0 36,127 - -0.8 36,165 - +0.8 36,219 + EX31_RS25880 0.15 -0.2 36,266 - EX31_RS25880 0.36 +1.0 36,266 - EX31_RS25880 0.36 -0.2 36,266 - EX31_RS25880 0.36 +1.2 36,266 - EX31_RS25880 0.36 +0.4 36,266 - EX31_RS25880 0.36 -3.0 36,315 + EX31_RS25880 0.58 +0.5 36,315 + EX31_RS25880 0.58 -0.6 36,372 - EX31_RS25880 0.84 +0.7 36,372 - EX31_RS25880 0.84 -0.0 36,372 - EX31_RS25880 0.84 +0.1 36,372 - EX31_RS25880 0.84 -1.3 36,372 - EX31_RS25880 0.84 -0.0 36,437 + +1.7 36,437 + -1.4 36,437 + +0.5 36,438 - -0.0 36,438 - +1.8 36,438 - +0.8 36,438 - +2.1 36,438 - -0.5 36,438 - -0.3 36,438 - -0.2 36,438 - -0.5 36,459 - +1.4 36,481 - -0.8 36,481 - +0.4 36,481 - +1.5 36,662 - -0.3 36,663 + -0.5 36,724 + +0.8 36,757 - -0.9 36,757 - +0.8 36,774 + -0.0 36,818 - EX31_RS00175 0.10 +1.1 36,892 + EX31_RS00175 0.16 +0.1 36,986 - EX31_RS00175 0.23 -0.1 36,995 - EX31_RS00175 0.24 +1.4 36,995 - EX31_RS00175 0.24 +0.7 36,997 + EX31_RS00175 0.24 +1.3 37,030 + EX31_RS00175 0.27 +1.1
Or see this region's nucleotide sequence