Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS15905 and AZOBR_RS15910 are separated by 32 nucleotides AZOBR_RS15910 and AZOBR_RS15915 are separated by 164 nucleotides
AZOBR_RS15905: AZOBR_RS15905 - 2-hydroxyhepta-2 4-diene-1 7-dioate isomerase, at 321,863 to 322,714
_RS15905
AZOBR_RS15910: AZOBR_RS15910 - hypothetical protein, at 322,747 to 324,030
_RS15910
AZOBR_RS15915: AZOBR_RS15915 - gluconate TRAP transporter, periplasmic solute-binding component <small>(from data)</small>, at 324,195 to 325,208
_RS15915
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -1
0
1 at 321.748 kb on + strand at 321.748 kb on + strand at 321.749 kb on - strand at 321.857 kb on - strand at 322.101 kb on - strand, within AZOBR_RS15905 at 322.538 kb on + strand, within AZOBR_RS15905 at 322.538 kb on + strand, within AZOBR_RS15905 at 322.539 kb on - strand, within AZOBR_RS15905 at 322.936 kb on + strand, within AZOBR_RS15910 at 322.936 kb on + strand, within AZOBR_RS15910 at 323.000 kb on - strand, within AZOBR_RS15910 at 323.215 kb on + strand, within AZOBR_RS15910 at 323.215 kb on + strand, within AZOBR_RS15910 at 323.692 kb on + strand, within AZOBR_RS15910 at 323.788 kb on + strand, within AZOBR_RS15910 at 323.789 kb on - strand, within AZOBR_RS15910 at 323.922 kb on + strand at 324.197 kb on - strand at 324.497 kb on - strand, within AZOBR_RS15915 at 324.520 kb on + strand, within AZOBR_RS15915 at 324.521 kb on - strand, within AZOBR_RS15915 at 324.521 kb on - strand, within AZOBR_RS15915 at 324.535 kb on + strand, within AZOBR_RS15915 at 324.536 kb on - strand, within AZOBR_RS15915 at 324.667 kb on + strand, within AZOBR_RS15915 at 324.668 kb on - strand, within AZOBR_RS15915
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 321,748 + -0.1 321,748 + -0.5 321,749 - +0.5 321,857 - -0.0 322,101 - AZOBR_RS15905 0.28 -0.4 322,538 + AZOBR_RS15905 0.79 +0.1 322,538 + AZOBR_RS15905 0.79 +0.5 322,539 - AZOBR_RS15905 0.79 +0.3 322,936 + AZOBR_RS15910 0.15 -0.3 322,936 + AZOBR_RS15910 0.15 -0.7 323,000 - AZOBR_RS15910 0.20 +0.1 323,215 + AZOBR_RS15910 0.36 +0.2 323,215 + AZOBR_RS15910 0.36 -0.2 323,692 + AZOBR_RS15910 0.74 -0.2 323,788 + AZOBR_RS15910 0.81 +0.2 323,789 - AZOBR_RS15910 0.81 -0.0 323,922 + +0.3 324,197 - +0.6 324,497 - AZOBR_RS15915 0.30 -0.1 324,520 + AZOBR_RS15915 0.32 +0.3 324,521 - AZOBR_RS15915 0.32 +0.2 324,521 - AZOBR_RS15915 0.32 +0.4 324,535 + AZOBR_RS15915 0.34 -1.2 324,536 - AZOBR_RS15915 0.34 -0.2 324,667 + AZOBR_RS15915 0.47 +1.2 324,668 - AZOBR_RS15915 0.47 +0.2
Or see this region's nucleotide sequence