Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07125 and MMP_RS07130 are separated by 14 nucleotides MMP_RS07130 and MMP_RS07135 are separated by 33 nucleotides MMP_RS07135 and MMP_RS07140 are separated by 108 nucleotides
MMP_RS07125: MMP_RS07125 - coenzyme F420-reducing hydrogenase FrhD protein, at 1,363,817 to 1,364,341
_RS07125
MMP_RS07130: MMP_RS07130 - coenzyme F420 hydrogenase subunit gamma, at 1,364,356 to 1,365,042
_RS07130
MMP_RS07135: MMP_RS07135 - coenzyme F420 hydrogenase subunit beta, at 1,365,076 to 1,365,924
_RS07135
MMP_RS07140: MMP_RS07140 - TIGR00341 family protein, at 1,366,033 to 1,367,040
_RS07140
Position (kb)
1365
1366 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1364.098 kb on - strand, within MMP_RS07125 at 1364.176 kb on + strand, within MMP_RS07125 at 1364.308 kb on + strand at 1364.381 kb on - strand at 1364.508 kb on + strand, within MMP_RS07130 at 1364.513 kb on - strand, within MMP_RS07130 at 1364.594 kb on - strand, within MMP_RS07130 at 1364.598 kb on + strand, within MMP_RS07130 at 1364.631 kb on - strand, within MMP_RS07130 at 1364.753 kb on - strand, within MMP_RS07130 at 1364.754 kb on + strand, within MMP_RS07130 at 1364.798 kb on - strand, within MMP_RS07130 at 1364.798 kb on - strand, within MMP_RS07130 at 1364.807 kb on + strand, within MMP_RS07130 at 1364.928 kb on + strand, within MMP_RS07130 at 1364.928 kb on + strand, within MMP_RS07130 at 1364.932 kb on + strand, within MMP_RS07130 at 1365.035 kb on - strand at 1365.040 kb on - strand at 1365.040 kb on - strand at 1365.040 kb on - strand at 1365.098 kb on - strand at 1365.188 kb on - strand, within MMP_RS07135 at 1365.262 kb on + strand, within MMP_RS07135 at 1365.304 kb on + strand, within MMP_RS07135 at 1365.459 kb on + strand, within MMP_RS07135 at 1365.463 kb on + strand, within MMP_RS07135 at 1365.470 kb on - strand, within MMP_RS07135 at 1365.494 kb on + strand, within MMP_RS07135 at 1365.494 kb on + strand, within MMP_RS07135 at 1365.494 kb on + strand, within MMP_RS07135 at 1365.502 kb on + strand, within MMP_RS07135 at 1365.587 kb on + strand, within MMP_RS07135 at 1365.659 kb on - strand, within MMP_RS07135 at 1365.659 kb on - strand, within MMP_RS07135 at 1365.664 kb on + strand, within MMP_RS07135 at 1365.671 kb on + strand, within MMP_RS07135 at 1365.885 kb on + strand at 1365.922 kb on - strand at 1365.976 kb on + strand at 1365.976 kb on + strand at 1365.979 kb on + strand at 1365.982 kb on - strand at 1365.983 kb on + strand at 1365.993 kb on - strand at 1366.031 kb on + strand at 1366.058 kb on + strand at 1366.058 kb on + strand at 1366.058 kb on + strand at 1366.058 kb on + strand at 1366.058 kb on + strand at 1366.120 kb on + strand at 1366.151 kb on + strand, within MMP_RS07140 at 1366.153 kb on + strand, within MMP_RS07140 at 1366.157 kb on - strand, within MMP_RS07140 at 1366.162 kb on + strand, within MMP_RS07140 at 1366.166 kb on - strand, within MMP_RS07140 at 1366.166 kb on - strand, within MMP_RS07140 at 1366.206 kb on + strand, within MMP_RS07140 at 1366.220 kb on + strand, within MMP_RS07140 at 1366.309 kb on + strand, within MMP_RS07140 at 1366.467 kb on + strand, within MMP_RS07140 at 1366.571 kb on - strand, within MMP_RS07140 at 1366.572 kb on + strand, within MMP_RS07140 at 1366.577 kb on - strand, within MMP_RS07140 at 1366.584 kb on + strand, within MMP_RS07140 at 1366.611 kb on + strand, within MMP_RS07140 at 1366.616 kb on - strand, within MMP_RS07140 at 1366.616 kb on - strand, within MMP_RS07140 at 1366.616 kb on - strand, within MMP_RS07140 at 1366.617 kb on + strand, within MMP_RS07140 at 1366.617 kb on + strand, within MMP_RS07140 at 1366.617 kb on + strand, within MMP_RS07140 at 1366.617 kb on + strand, within MMP_RS07140 at 1366.638 kb on + strand, within MMP_RS07140 at 1366.638 kb on + strand, within MMP_RS07140 at 1366.656 kb on + strand, within MMP_RS07140 at 1366.716 kb on + strand, within MMP_RS07140 at 1366.749 kb on - strand, within MMP_RS07140 at 1366.813 kb on - strand, within MMP_RS07140 at 1366.813 kb on - strand, within MMP_RS07140 at 1366.814 kb on + strand, within MMP_RS07140 at 1366.814 kb on + strand, within MMP_RS07140 at 1366.818 kb on + strand, within MMP_RS07140 at 1366.841 kb on - strand, within MMP_RS07140 at 1366.841 kb on - strand, within MMP_RS07140 at 1366.846 kb on + strand, within MMP_RS07140 at 1366.854 kb on - strand, within MMP_RS07140 at 1366.855 kb on + strand, within MMP_RS07140 at 1366.856 kb on - strand, within MMP_RS07140 at 1366.913 kb on + strand, within MMP_RS07140 at 1366.914 kb on - strand, within MMP_RS07140 at 1366.915 kb on + strand, within MMP_RS07140
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations remove 1,364,098 - MMP_RS07125 0.54 -1.4 1,364,176 + MMP_RS07125 0.68 -1.3 1,364,308 + -1.7 1,364,381 - -0.5 1,364,508 + MMP_RS07130 0.22 -1.9 1,364,513 - MMP_RS07130 0.23 -1.0 1,364,594 - MMP_RS07130 0.35 -1.5 1,364,598 + MMP_RS07130 0.35 -1.4 1,364,631 - MMP_RS07130 0.40 -1.8 1,364,753 - MMP_RS07130 0.58 -1.7 1,364,754 + MMP_RS07130 0.58 -1.8 1,364,798 - MMP_RS07130 0.64 -1.8 1,364,798 - MMP_RS07130 0.64 -1.7 1,364,807 + MMP_RS07130 0.66 -1.4 1,364,928 + MMP_RS07130 0.83 -1.3 1,364,928 + MMP_RS07130 0.83 -2.7 1,364,932 + MMP_RS07130 0.84 -1.7 1,365,035 - -2.9 1,365,040 - -2.2 1,365,040 - -2.5 1,365,040 - -2.9 1,365,098 - -1.5 1,365,188 - MMP_RS07135 0.13 -1.7 1,365,262 + MMP_RS07135 0.22 -1.9 1,365,304 + MMP_RS07135 0.27 -1.8 1,365,459 + MMP_RS07135 0.45 -2.1 1,365,463 + MMP_RS07135 0.46 -1.7 1,365,470 - MMP_RS07135 0.46 -2.1 1,365,494 + MMP_RS07135 0.49 -1.7 1,365,494 + MMP_RS07135 0.49 -3.2 1,365,494 + MMP_RS07135 0.49 -1.6 1,365,502 + MMP_RS07135 0.50 -2.0 1,365,587 + MMP_RS07135 0.60 -1.8 1,365,659 - MMP_RS07135 0.69 -1.2 1,365,659 - MMP_RS07135 0.69 -2.6 1,365,664 + MMP_RS07135 0.69 -2.1 1,365,671 + MMP_RS07135 0.70 -2.0 1,365,885 + +0.2 1,365,922 - +0.1 1,365,976 + +0.2 1,365,976 + +0.2 1,365,979 + +0.9 1,365,982 - +1.1 1,365,983 + +0.6 1,365,993 - +0.2 1,366,031 + +0.6 1,366,058 + +0.5 1,366,058 + +1.0 1,366,058 + +0.4 1,366,058 + +0.5 1,366,058 + +1.0 1,366,120 + +1.0 1,366,151 + MMP_RS07140 0.12 +0.7 1,366,153 + MMP_RS07140 0.12 +0.4 1,366,157 - MMP_RS07140 0.12 +0.0 1,366,162 + MMP_RS07140 0.13 +0.1 1,366,166 - MMP_RS07140 0.13 +0.3 1,366,166 - MMP_RS07140 0.13 +0.5 1,366,206 + MMP_RS07140 0.17 +0.8 1,366,220 + MMP_RS07140 0.19 +0.3 1,366,309 + MMP_RS07140 0.27 +0.9 1,366,467 + MMP_RS07140 0.43 -0.0 1,366,571 - MMP_RS07140 0.53 +0.3 1,366,572 + MMP_RS07140 0.53 +0.8 1,366,577 - MMP_RS07140 0.54 +0.2 1,366,584 + MMP_RS07140 0.55 +0.3 1,366,611 + MMP_RS07140 0.57 +0.3 1,366,616 - MMP_RS07140 0.58 +0.2 1,366,616 - MMP_RS07140 0.58 -0.2 1,366,616 - MMP_RS07140 0.58 +0.3 1,366,617 + MMP_RS07140 0.58 +0.2 1,366,617 + MMP_RS07140 0.58 +1.0 1,366,617 + MMP_RS07140 0.58 +0.9 1,366,617 + MMP_RS07140 0.58 -0.2 1,366,638 + MMP_RS07140 0.60 +0.8 1,366,638 + MMP_RS07140 0.60 +0.9 1,366,656 + MMP_RS07140 0.62 +0.5 1,366,716 + MMP_RS07140 0.68 +0.8 1,366,749 - MMP_RS07140 0.71 +0.8 1,366,813 - MMP_RS07140 0.77 +1.1 1,366,813 - MMP_RS07140 0.77 +0.5 1,366,814 + MMP_RS07140 0.77 +0.6 1,366,814 + MMP_RS07140 0.77 +0.3 1,366,818 + MMP_RS07140 0.78 +1.0 1,366,841 - MMP_RS07140 0.80 +0.5 1,366,841 - MMP_RS07140 0.80 +0.3 1,366,846 + MMP_RS07140 0.81 +0.4 1,366,854 - MMP_RS07140 0.81 +0.8 1,366,855 + MMP_RS07140 0.82 +1.0 1,366,856 - MMP_RS07140 0.82 +0.7 1,366,913 + MMP_RS07140 0.87 +0.8 1,366,914 - MMP_RS07140 0.87 +0.5 1,366,915 + MMP_RS07140 0.88 +0.4
Or see this region's nucleotide sequence