Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13065 and EX31_RS13070 are separated by 1 nucleotides EX31_RS13070 and EX31_RS13075 are separated by 190 nucleotides EX31_RS13075 and EX31_RS13080 are separated by 658 nucleotides
EX31_RS13065: EX31_RS13065 - tRNA pseudouridine(65) synthase TruC, at 39,597 to 40,376
_RS13065
EX31_RS13070: EX31_RS13070 - YqcC family protein, at 40,378 to 40,692
_RS13070
EX31_RS13075: EX31_RS13075 - MFS transporter, at 40,883 to 42,121
_RS13075
EX31_RS13080: EX31_RS13080 - SecY-interacting protein, at 42,780 to 43,331
_RS13080
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 39.897 kb on - strand, within EX31_RS13065 at 39.897 kb on - strand, within EX31_RS13065 at 39.929 kb on + strand, within EX31_RS13065 at 39.929 kb on + strand, within EX31_RS13065 at 39.929 kb on + strand, within EX31_RS13065 at 39.930 kb on - strand, within EX31_RS13065 at 39.930 kb on - strand, within EX31_RS13065 at 39.941 kb on + strand, within EX31_RS13065 at 39.941 kb on + strand, within EX31_RS13065 at 39.941 kb on + strand, within EX31_RS13065 at 39.946 kb on + strand, within EX31_RS13065 at 40.019 kb on + strand, within EX31_RS13065 at 40.073 kb on - strand, within EX31_RS13065 at 40.100 kb on - strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.120 kb on + strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.121 kb on - strand, within EX31_RS13065 at 40.161 kb on + strand, within EX31_RS13065 at 40.162 kb on - strand, within EX31_RS13065 at 40.162 kb on - strand, within EX31_RS13065 at 40.232 kb on + strand, within EX31_RS13065 at 40.319 kb on + strand at 40.319 kb on + strand at 40.331 kb on + strand at 40.332 kb on - strand at 40.335 kb on + strand at 40.335 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.387 kb on + strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.388 kb on - strand at 40.460 kb on + strand, within EX31_RS13070 at 40.471 kb on - strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.514 kb on + strand, within EX31_RS13070 at 40.515 kb on - strand, within EX31_RS13070 at 40.515 kb on - strand, within EX31_RS13070 at 40.515 kb on - strand, within EX31_RS13070 at 40.515 kb on - strand, within EX31_RS13070 at 40.515 kb on - strand, within EX31_RS13070 at 40.518 kb on + strand, within EX31_RS13070 at 40.519 kb on - strand, within EX31_RS13070 at 40.519 kb on - strand, within EX31_RS13070 at 40.519 kb on - strand, within EX31_RS13070 at 40.684 kb on + strand at 40.685 kb on - strand at 40.685 kb on - strand at 40.685 kb on - strand at 40.685 kb on - strand at 40.687 kb on - strand at 40.748 kb on + strand at 40.749 kb on - strand at 40.749 kb on - strand at 40.785 kb on + strand at 40.786 kb on - strand at 40.794 kb on + strand at 40.794 kb on + strand at 40.794 kb on + strand at 40.794 kb on + strand at 40.795 kb on - strand at 40.795 kb on - strand at 40.795 kb on - strand at 40.798 kb on + strand at 40.799 kb on - strand at 40.808 kb on + strand at 40.808 kb on + strand at 40.809 kb on - strand at 40.809 kb on - strand at 40.809 kb on - strand at 40.809 kb on - strand at 40.809 kb on - strand at 40.828 kb on + strand at 40.851 kb on - strand at 40.851 kb on - strand at 40.953 kb on - strand at 40.975 kb on - strand at 41.002 kb on + strand at 41.006 kb on - strand at 41.189 kb on + strand, within EX31_RS13075 at 41.190 kb on - strand, within EX31_RS13075 at 41.199 kb on + strand, within EX31_RS13075 at 41.199 kb on + strand, within EX31_RS13075 at 41.199 kb on + strand, within EX31_RS13075 at 41.199 kb on + strand, within EX31_RS13075 at 41.199 kb on + strand, within EX31_RS13075 at 41.200 kb on - strand, within EX31_RS13075 at 41.200 kb on - strand, within EX31_RS13075 at 41.200 kb on - strand, within EX31_RS13075 at 41.207 kb on + strand, within EX31_RS13075 at 41.207 kb on + strand, within EX31_RS13075 at 41.208 kb on - strand, within EX31_RS13075 at 41.309 kb on + strand, within EX31_RS13075 at 41.309 kb on + strand, within EX31_RS13075 at 41.309 kb on + strand, within EX31_RS13075 at 41.309 kb on + strand, within EX31_RS13075 at 41.310 kb on - strand, within EX31_RS13075 at 41.310 kb on - strand, within EX31_RS13075 at 41.310 kb on - strand, within EX31_RS13075 at 41.310 kb on - strand, within EX31_RS13075 at 41.310 kb on - strand, within EX31_RS13075 at 41.341 kb on - strand, within EX31_RS13075 at 41.393 kb on - strand, within EX31_RS13075 at 41.488 kb on + strand, within EX31_RS13075 at 41.489 kb on - strand, within EX31_RS13075 at 41.516 kb on - strand, within EX31_RS13075 at 41.516 kb on - strand, within EX31_RS13075 at 41.521 kb on - strand, within EX31_RS13075 at 41.521 kb on - strand, within EX31_RS13075 at 41.594 kb on + strand, within EX31_RS13075 at 41.597 kb on + strand, within EX31_RS13075 at 41.624 kb on + strand, within EX31_RS13075 at 41.624 kb on + strand, within EX31_RS13075 at 41.625 kb on - strand, within EX31_RS13075 at 41.625 kb on - strand, within EX31_RS13075 at 41.625 kb on - strand, within EX31_RS13075 at 41.625 kb on - strand, within EX31_RS13075 at 41.692 kb on + strand, within EX31_RS13075 at 41.692 kb on + strand, within EX31_RS13075 at 41.693 kb on - strand, within EX31_RS13075 at 41.763 kb on - strand, within EX31_RS13075 at 41.787 kb on + strand, within EX31_RS13075 at 41.788 kb on - strand, within EX31_RS13075 at 41.788 kb on - strand, within EX31_RS13075 at 41.796 kb on - strand, within EX31_RS13075 at 41.907 kb on + strand, within EX31_RS13075 at 41.907 kb on + strand, within EX31_RS13075 at 41.937 kb on + strand, within EX31_RS13075 at 41.938 kb on - strand, within EX31_RS13075 at 41.941 kb on + strand, within EX31_RS13075 at 41.941 kb on + strand, within EX31_RS13075 at 41.941 kb on + strand, within EX31_RS13075 at 41.941 kb on + strand, within EX31_RS13075 at 41.942 kb on - strand, within EX31_RS13075 at 42.010 kb on + strand at 42.022 kb on + strand at 42.022 kb on + strand at 42.023 kb on - strand at 42.023 kb on - strand at 42.042 kb on - strand at 42.175 kb on + strand at 42.181 kb on + strand at 42.182 kb on - strand at 42.182 kb on - strand at 42.225 kb on - strand at 42.227 kb on - strand at 42.239 kb on - strand at 42.338 kb on - strand at 42.338 kb on - strand at 42.338 kb on - strand at 42.338 kb on - strand at 42.450 kb on - strand at 42.450 kb on - strand at 42.605 kb on - strand at 42.675 kb on + strand at 42.676 kb on - strand at 42.809 kb on + strand at 42.809 kb on + strand at 42.809 kb on + strand at 42.809 kb on + strand at 42.809 kb on + strand at 42.810 kb on - strand at 42.810 kb on - strand at 42.837 kb on + strand, within EX31_RS13080 at 42.837 kb on + strand, within EX31_RS13080 at 42.837 kb on + strand, within EX31_RS13080 at 42.837 kb on + strand, within EX31_RS13080 at 42.837 kb on + strand, within EX31_RS13080 at 42.837 kb on + strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.838 kb on - strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.839 kb on + strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.840 kb on - strand, within EX31_RS13080 at 42.879 kb on - strand, within EX31_RS13080 at 43.078 kb on + strand, within EX31_RS13080 at 43.078 kb on + strand, within EX31_RS13080 at 43.079 kb on - strand, within EX31_RS13080 at 43.079 kb on - strand, within EX31_RS13080 at 43.079 kb on - strand, within EX31_RS13080 at 43.091 kb on - strand, within EX31_RS13080 at 43.119 kb on + strand, within EX31_RS13080 at 43.120 kb on - strand, within EX31_RS13080
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 39,897 - EX31_RS13065 0.38 +0.4 39,897 - EX31_RS13065 0.38 -0.4 39,929 + EX31_RS13065 0.43 -0.6 39,929 + EX31_RS13065 0.43 +1.2 39,929 + EX31_RS13065 0.43 +0.4 39,930 - EX31_RS13065 0.43 -0.9 39,930 - EX31_RS13065 0.43 -0.5 39,941 + EX31_RS13065 0.44 +1.0 39,941 + EX31_RS13065 0.44 -0.5 39,941 + EX31_RS13065 0.44 +0.3 39,946 + EX31_RS13065 0.45 -0.2 40,019 + EX31_RS13065 0.54 -0.4 40,073 - EX31_RS13065 0.61 -0.4 40,100 - EX31_RS13065 0.64 -0.0 40,120 + EX31_RS13065 0.67 -0.9 40,120 + EX31_RS13065 0.67 +0.0 40,120 + EX31_RS13065 0.67 +0.1 40,120 + EX31_RS13065 0.67 +0.4 40,120 + EX31_RS13065 0.67 +0.2 40,120 + EX31_RS13065 0.67 -0.6 40,120 + EX31_RS13065 0.67 -0.1 40,120 + EX31_RS13065 0.67 +0.4 40,121 - EX31_RS13065 0.67 +1.0 40,121 - EX31_RS13065 0.67 -0.9 40,121 - EX31_RS13065 0.67 +0.5 40,121 - EX31_RS13065 0.67 +0.4 40,121 - EX31_RS13065 0.67 -0.9 40,121 - EX31_RS13065 0.67 +0.1 40,121 - EX31_RS13065 0.67 +0.9 40,121 - EX31_RS13065 0.67 -0.2 40,121 - EX31_RS13065 0.67 -0.4 40,121 - EX31_RS13065 0.67 +0.1 40,121 - EX31_RS13065 0.67 -0.9 40,121 - EX31_RS13065 0.67 -0.9 40,121 - EX31_RS13065 0.67 +0.5 40,121 - EX31_RS13065 0.67 +0.2 40,121 - EX31_RS13065 0.67 +0.4 40,121 - EX31_RS13065 0.67 -0.1 40,121 - EX31_RS13065 0.67 -2.0 40,121 - EX31_RS13065 0.67 +0.5 40,121 - EX31_RS13065 0.67 +0.4 40,161 + EX31_RS13065 0.72 +0.3 40,162 - EX31_RS13065 0.72 -0.5 40,162 - EX31_RS13065 0.72 -0.2 40,232 + EX31_RS13065 0.81 +0.7 40,319 + -0.0 40,319 + -0.1 40,331 + +0.7 40,332 - -0.7 40,335 + -0.1 40,335 + -0.1 40,387 + -0.2 40,387 + -1.1 40,387 + -0.7 40,387 + -0.2 40,387 + +0.7 40,387 + +0.0 40,387 + -0.3 40,388 - -0.5 40,388 - +0.5 40,388 - -0.4 40,388 - -0.9 40,388 - +0.2 40,388 - +0.0 40,388 - +0.3 40,388 - +0.3 40,388 - -0.3 40,388 - -0.1 40,388 - -0.3 40,388 - -0.0 40,460 + EX31_RS13070 0.26 +1.1 40,471 - EX31_RS13070 0.30 +1.3 40,514 + EX31_RS13070 0.43 +1.2 40,514 + EX31_RS13070 0.43 +2.9 40,514 + EX31_RS13070 0.43 +1.3 40,514 + EX31_RS13070 0.43 -0.2 40,514 + EX31_RS13070 0.43 -0.3 40,514 + EX31_RS13070 0.43 -0.7 40,514 + EX31_RS13070 0.43 +0.4 40,515 - EX31_RS13070 0.43 +0.7 40,515 - EX31_RS13070 0.43 +1.5 40,515 - EX31_RS13070 0.43 -0.0 40,515 - EX31_RS13070 0.43 +0.9 40,515 - EX31_RS13070 0.43 -0.1 40,518 + EX31_RS13070 0.44 -0.8 40,519 - EX31_RS13070 0.45 -0.3 40,519 - EX31_RS13070 0.45 +1.8 40,519 - EX31_RS13070 0.45 +1.1 40,684 + +0.1 40,685 - +1.0 40,685 - -0.2 40,685 - +0.2 40,685 - +0.4 40,687 - +0.8 40,748 + -0.7 40,749 - -0.4 40,749 - +1.3 40,785 + +0.7 40,786 - +0.3 40,794 + +1.2 40,794 + -0.7 40,794 + +0.1 40,794 + +1.2 40,795 - +1.5 40,795 - -0.0 40,795 - -1.0 40,798 + -0.7 40,799 - +0.1 40,808 + +0.5 40,808 + -0.1 40,809 - -0.4 40,809 - +0.2 40,809 - +0.6 40,809 - -0.5 40,809 - -0.8 40,828 + +0.3 40,851 - +0.1 40,851 - -0.1 40,953 - -0.5 40,975 - -0.5 41,002 + +0.3 41,006 - -0.2 41,189 + EX31_RS13075 0.25 +1.5 41,190 - EX31_RS13075 0.25 -0.2 41,199 + EX31_RS13075 0.26 +0.2 41,199 + EX31_RS13075 0.26 -0.0 41,199 + EX31_RS13075 0.26 +0.3 41,199 + EX31_RS13075 0.26 +0.1 41,199 + EX31_RS13075 0.26 +0.1 41,200 - EX31_RS13075 0.26 -0.6 41,200 - EX31_RS13075 0.26 +0.5 41,200 - EX31_RS13075 0.26 -0.2 41,207 + EX31_RS13075 0.26 +0.6 41,207 + EX31_RS13075 0.26 -0.8 41,208 - EX31_RS13075 0.26 +0.2 41,309 + EX31_RS13075 0.34 -0.7 41,309 + EX31_RS13075 0.34 -0.9 41,309 + EX31_RS13075 0.34 +0.5 41,309 + EX31_RS13075 0.34 +0.2 41,310 - EX31_RS13075 0.34 -1.2 41,310 - EX31_RS13075 0.34 -1.7 41,310 - EX31_RS13075 0.34 -0.0 41,310 - EX31_RS13075 0.34 -0.6 41,310 - EX31_RS13075 0.34 -0.1 41,341 - EX31_RS13075 0.37 +1.4 41,393 - EX31_RS13075 0.41 +0.0 41,488 + EX31_RS13075 0.49 -0.5 41,489 - EX31_RS13075 0.49 -0.1 41,516 - EX31_RS13075 0.51 +0.6 41,516 - EX31_RS13075 0.51 +1.3 41,521 - EX31_RS13075 0.51 -0.0 41,521 - EX31_RS13075 0.51 -0.2 41,594 + EX31_RS13075 0.57 -0.4 41,597 + EX31_RS13075 0.58 -0.5 41,624 + EX31_RS13075 0.60 -0.3 41,624 + EX31_RS13075 0.60 -0.3 41,625 - EX31_RS13075 0.60 -0.7 41,625 - EX31_RS13075 0.60 +0.4 41,625 - EX31_RS13075 0.60 +0.3 41,625 - EX31_RS13075 0.60 +0.7 41,692 + EX31_RS13075 0.65 -1.2 41,692 + EX31_RS13075 0.65 -0.9 41,693 - EX31_RS13075 0.65 +0.6 41,763 - EX31_RS13075 0.71 +1.0 41,787 + EX31_RS13075 0.73 +0.3 41,788 - EX31_RS13075 0.73 +0.3 41,788 - EX31_RS13075 0.73 +0.4 41,796 - EX31_RS13075 0.74 +0.5 41,907 + EX31_RS13075 0.83 -0.9 41,907 + EX31_RS13075 0.83 -0.9 41,937 + EX31_RS13075 0.85 -0.3 41,938 - EX31_RS13075 0.85 +1.1 41,941 + EX31_RS13075 0.85 +1.8 41,941 + EX31_RS13075 0.85 +0.5 41,941 + EX31_RS13075 0.85 -0.4 41,941 + EX31_RS13075 0.85 -0.2 41,942 - EX31_RS13075 0.85 -0.5 42,010 + -0.2 42,022 + +0.3 42,022 + +1.6 42,023 - +0.2 42,023 - +1.7 42,042 - +0.3 42,175 + +0.2 42,181 + +1.0 42,182 - +0.7 42,182 - -0.3 42,225 - -0.1 42,227 - -0.6 42,239 - +0.1 42,338 - -0.5 42,338 - -0.4 42,338 - -0.1 42,338 - -1.1 42,450 - -0.1 42,450 - -0.4 42,605 - +0.7 42,675 + +0.3 42,676 - +2.6 42,809 + -1.7 42,809 + -0.3 42,809 + +0.3 42,809 + -0.4 42,809 + +0.1 42,810 - +0.3 42,810 - -0.5 42,837 + EX31_RS13080 0.10 -0.1 42,837 + EX31_RS13080 0.10 +0.0 42,837 + EX31_RS13080 0.10 -0.9 42,837 + EX31_RS13080 0.10 +0.4 42,837 + EX31_RS13080 0.10 -0.4 42,837 + EX31_RS13080 0.10 +0.6 42,838 - EX31_RS13080 0.11 -0.2 42,838 - EX31_RS13080 0.11 -0.0 42,838 - EX31_RS13080 0.11 +0.1 42,838 - EX31_RS13080 0.11 -0.2 42,838 - EX31_RS13080 0.11 -1.3 42,838 - EX31_RS13080 0.11 +0.7 42,839 + EX31_RS13080 0.11 -0.9 42,839 + EX31_RS13080 0.11 +1.4 42,839 + EX31_RS13080 0.11 -0.2 42,839 + EX31_RS13080 0.11 -1.0 42,839 + EX31_RS13080 0.11 -0.8 42,839 + EX31_RS13080 0.11 -0.2 42,839 + EX31_RS13080 0.11 -0.0 42,839 + EX31_RS13080 0.11 -0.8 42,839 + EX31_RS13080 0.11 +0.5 42,840 - EX31_RS13080 0.11 +0.9 42,840 - EX31_RS13080 0.11 +0.9 42,840 - EX31_RS13080 0.11 -0.2 42,840 - EX31_RS13080 0.11 +0.0 42,840 - EX31_RS13080 0.11 +1.4 42,840 - EX31_RS13080 0.11 +0.1 42,840 - EX31_RS13080 0.11 -0.9 42,840 - EX31_RS13080 0.11 -0.4 42,840 - EX31_RS13080 0.11 +1.9 42,840 - EX31_RS13080 0.11 -0.8 42,840 - EX31_RS13080 0.11 +2.0 42,840 - EX31_RS13080 0.11 -0.4 42,840 - EX31_RS13080 0.11 -0.8 42,840 - EX31_RS13080 0.11 +0.5 42,840 - EX31_RS13080 0.11 -0.8 42,879 - EX31_RS13080 0.18 +0.4 43,078 + EX31_RS13080 0.54 +1.1 43,078 + EX31_RS13080 0.54 -0.2 43,079 - EX31_RS13080 0.54 +0.2 43,079 - EX31_RS13080 0.54 +0.5 43,079 - EX31_RS13080 0.54 +0.2 43,091 - EX31_RS13080 0.56 -1.4 43,119 + EX31_RS13080 0.61 +0.4 43,120 - EX31_RS13080 0.62 +0.8
Or see this region's nucleotide sequence