Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11145 and EX31_RS11150 are separated by 229 nucleotides EX31_RS11150 and EX31_RS11155 overlap by 27 nucleotides EX31_RS11155 and EX31_RS11160 are separated by 28 nucleotides
EX31_RS11145: EX31_RS11145 - LLM class flavin-dependent oxidoreductase, at 160,788 to 161,813
_RS11145
EX31_RS11150: EX31_RS11150 - efflux transporter periplasmic adaptor subunit, at 162,043 to 162,312
_RS11150
EX31_RS11155: EX31_RS11155 - DHA2 family efflux MFS transporter permease subunit, at 162,286 to 163,668
_RS11155
EX31_RS11160: EX31_RS11160 - hypothetical protein, at 163,697 to 164,677
_RS11160
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 161.548 kb on + strand, within EX31_RS11145 at 161.548 kb on + strand, within EX31_RS11145 at 161.548 kb on + strand, within EX31_RS11145 at 161.548 kb on + strand, within EX31_RS11145 at 161.548 kb on + strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.549 kb on - strand, within EX31_RS11145 at 161.734 kb on - strand at 161.875 kb on + strand at 161.887 kb on + strand at 161.888 kb on - strand at 161.888 kb on - strand at 161.933 kb on - strand at 161.933 kb on - strand at 161.933 kb on - strand at 161.953 kb on + strand at 162.044 kb on + strand at 162.078 kb on + strand, within EX31_RS11150 at 162.078 kb on + strand, within EX31_RS11150 at 162.079 kb on - strand, within EX31_RS11150 at 162.165 kb on - strand, within EX31_RS11150 at 162.171 kb on + strand, within EX31_RS11150 at 162.172 kb on - strand, within EX31_RS11150 at 162.209 kb on + strand, within EX31_RS11150 at 162.210 kb on - strand, within EX31_RS11150 at 162.265 kb on - strand, within EX31_RS11150 at 162.306 kb on + strand at 162.306 kb on + strand at 162.307 kb on - strand at 162.307 kb on - strand at 162.307 kb on - strand at 162.307 kb on - strand at 162.307 kb on - strand at 162.356 kb on + strand at 162.356 kb on + strand at 162.356 kb on + strand at 162.357 kb on - strand at 162.519 kb on + strand, within EX31_RS11155 at 162.520 kb on - strand, within EX31_RS11155 at 162.520 kb on - strand, within EX31_RS11155 at 162.546 kb on - strand, within EX31_RS11155 at 162.736 kb on - strand, within EX31_RS11155 at 162.736 kb on - strand, within EX31_RS11155 at 162.784 kb on + strand, within EX31_RS11155 at 162.856 kb on - strand, within EX31_RS11155 at 162.856 kb on - strand, within EX31_RS11155 at 162.874 kb on + strand, within EX31_RS11155 at 162.874 kb on + strand, within EX31_RS11155 at 162.876 kb on + strand, within EX31_RS11155 at 162.967 kb on + strand, within EX31_RS11155 at 163.134 kb on + strand, within EX31_RS11155 at 163.135 kb on - strand, within EX31_RS11155 at 163.136 kb on + strand, within EX31_RS11155 at 163.136 kb on + strand, within EX31_RS11155 at 163.136 kb on + strand, within EX31_RS11155 at 163.136 kb on + strand, within EX31_RS11155 at 163.137 kb on - strand, within EX31_RS11155 at 163.137 kb on - strand, within EX31_RS11155 at 163.137 kb on - strand, within EX31_RS11155 at 163.137 kb on - strand, within EX31_RS11155 at 163.248 kb on + strand, within EX31_RS11155 at 163.248 kb on + strand, within EX31_RS11155 at 163.264 kb on - strand, within EX31_RS11155 at 163.264 kb on - strand, within EX31_RS11155 at 163.271 kb on + strand, within EX31_RS11155 at 163.272 kb on - strand, within EX31_RS11155 at 163.304 kb on + strand, within EX31_RS11155 at 163.304 kb on + strand, within EX31_RS11155 at 163.305 kb on - strand, within EX31_RS11155 at 163.321 kb on + strand, within EX31_RS11155 at 163.321 kb on + strand, within EX31_RS11155 at 163.321 kb on + strand, within EX31_RS11155 at 163.322 kb on - strand, within EX31_RS11155 at 163.322 kb on - strand, within EX31_RS11155 at 163.322 kb on - strand, within EX31_RS11155 at 163.322 kb on - strand, within EX31_RS11155 at 163.363 kb on - strand, within EX31_RS11155 at 163.406 kb on + strand, within EX31_RS11155 at 163.406 kb on + strand, within EX31_RS11155 at 163.406 kb on + strand, within EX31_RS11155 at 163.407 kb on - strand, within EX31_RS11155 at 163.514 kb on - strand, within EX31_RS11155 at 163.533 kb on + strand at 163.614 kb on - strand at 163.658 kb on - strand at 163.658 kb on - strand at 163.669 kb on - strand at 163.669 kb on - strand at 163.669 kb on - strand at 163.669 kb on - strand at 163.669 kb on - strand at 163.669 kb on - strand at 163.682 kb on + strand at 163.682 kb on + strand at 163.682 kb on + strand at 163.682 kb on + strand at 163.682 kb on + strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.683 kb on - strand at 163.699 kb on - strand at 163.699 kb on - strand at 163.816 kb on + strand, within EX31_RS11160 at 163.835 kb on - strand, within EX31_RS11160 at 163.852 kb on - strand, within EX31_RS11160 at 163.866 kb on + strand, within EX31_RS11160 at 163.867 kb on - strand, within EX31_RS11160 at 163.867 kb on - strand, within EX31_RS11160 at 163.867 kb on - strand, within EX31_RS11160 at 163.882 kb on + strand, within EX31_RS11160 at 163.883 kb on - strand, within EX31_RS11160 at 164.019 kb on - strand, within EX31_RS11160 at 164.138 kb on - strand, within EX31_RS11160 at 164.139 kb on + strand, within EX31_RS11160 at 164.139 kb on + strand, within EX31_RS11160 at 164.139 kb on + strand, within EX31_RS11160 at 164.139 kb on + strand, within EX31_RS11160 at 164.140 kb on - strand, within EX31_RS11160 at 164.272 kb on + strand, within EX31_RS11160 at 164.273 kb on - strand, within EX31_RS11160 at 164.496 kb on + strand, within EX31_RS11160 at 164.496 kb on + strand, within EX31_RS11160 at 164.557 kb on + strand, within EX31_RS11160 at 164.658 kb on - strand at 164.662 kb on + strand at 164.662 kb on + strand at 164.662 kb on + strand at 164.663 kb on - strand at 164.663 kb on - strand at 164.663 kb on - strand at 164.663 kb on - strand at 164.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 161,548 + EX31_RS11145 0.74 -0.1 161,548 + EX31_RS11145 0.74 +0.6 161,548 + EX31_RS11145 0.74 -1.7 161,548 + EX31_RS11145 0.74 -0.7 161,548 + EX31_RS11145 0.74 -2.2 161,549 - EX31_RS11145 0.74 +0.7 161,549 - EX31_RS11145 0.74 -1.2 161,549 - EX31_RS11145 0.74 -0.4 161,549 - EX31_RS11145 0.74 +0.7 161,549 - EX31_RS11145 0.74 +0.5 161,549 - EX31_RS11145 0.74 -0.2 161,549 - EX31_RS11145 0.74 +1.3 161,549 - EX31_RS11145 0.74 -0.0 161,549 - EX31_RS11145 0.74 -2.1 161,549 - EX31_RS11145 0.74 -1.1 161,734 - +2.1 161,875 + -0.1 161,887 + +0.9 161,888 - -0.2 161,888 - +1.3 161,933 - -1.1 161,933 - -0.6 161,933 - -0.1 161,953 + -1.2 162,044 + -0.2 162,078 + EX31_RS11150 0.13 +0.4 162,078 + EX31_RS11150 0.13 -0.7 162,079 - EX31_RS11150 0.13 +0.1 162,165 - EX31_RS11150 0.45 -0.7 162,171 + EX31_RS11150 0.47 +0.2 162,172 - EX31_RS11150 0.48 -0.7 162,209 + EX31_RS11150 0.61 -1.7 162,210 - EX31_RS11150 0.62 +1.2 162,265 - EX31_RS11150 0.82 -1.4 162,306 + -1.2 162,306 + +0.1 162,307 - +0.3 162,307 - +2.1 162,307 - -0.1 162,307 - +0.9 162,307 - -0.4 162,356 + +0.5 162,356 + -0.1 162,356 + -0.3 162,357 - +0.5 162,519 + EX31_RS11155 0.17 +0.7 162,520 - EX31_RS11155 0.17 -0.7 162,520 - EX31_RS11155 0.17 +0.1 162,546 - EX31_RS11155 0.19 -0.2 162,736 - EX31_RS11155 0.33 +0.7 162,736 - EX31_RS11155 0.33 +0.6 162,784 + EX31_RS11155 0.36 +1.9 162,856 - EX31_RS11155 0.41 -0.6 162,856 - EX31_RS11155 0.41 +1.5 162,874 + EX31_RS11155 0.43 +1.1 162,874 + EX31_RS11155 0.43 +0.1 162,876 + EX31_RS11155 0.43 +0.8 162,967 + EX31_RS11155 0.49 +1.6 163,134 + EX31_RS11155 0.61 +2.4 163,135 - EX31_RS11155 0.61 +0.4 163,136 + EX31_RS11155 0.61 +0.2 163,136 + EX31_RS11155 0.61 -1.3 163,136 + EX31_RS11155 0.61 -0.4 163,136 + EX31_RS11155 0.61 +0.6 163,137 - EX31_RS11155 0.62 +1.6 163,137 - EX31_RS11155 0.62 +1.1 163,137 - EX31_RS11155 0.62 +0.9 163,137 - EX31_RS11155 0.62 -0.9 163,248 + EX31_RS11155 0.70 +1.0 163,248 + EX31_RS11155 0.70 -0.1 163,264 - EX31_RS11155 0.71 +0.6 163,264 - EX31_RS11155 0.71 +1.9 163,271 + EX31_RS11155 0.71 +0.5 163,272 - EX31_RS11155 0.71 +1.0 163,304 + EX31_RS11155 0.74 -0.9 163,304 + EX31_RS11155 0.74 -0.1 163,305 - EX31_RS11155 0.74 -1.2 163,321 + EX31_RS11155 0.75 +1.2 163,321 + EX31_RS11155 0.75 -0.1 163,321 + EX31_RS11155 0.75 +2.9 163,322 - EX31_RS11155 0.75 +1.7 163,322 - EX31_RS11155 0.75 -0.2 163,322 - EX31_RS11155 0.75 +0.6 163,322 - EX31_RS11155 0.75 -1.2 163,363 - EX31_RS11155 0.78 -1.5 163,406 + EX31_RS11155 0.81 -0.7 163,406 + EX31_RS11155 0.81 -0.8 163,406 + EX31_RS11155 0.81 +1.1 163,407 - EX31_RS11155 0.81 -1.0 163,514 - EX31_RS11155 0.89 +0.5 163,533 + +1.3 163,614 - +0.3 163,658 - +0.2 163,658 - +1.9 163,669 - +0.3 163,669 - -0.5 163,669 - +2.5 163,669 - +0.2 163,669 - +0.2 163,669 - +0.6 163,682 + -0.1 163,682 + +0.1 163,682 + -0.2 163,682 + +1.1 163,682 + +0.9 163,683 - -2.4 163,683 - -0.9 163,683 - +3.3 163,683 - -0.3 163,683 - -0.9 163,683 - +1.3 163,683 - +1.7 163,699 - +1.2 163,699 - -0.1 163,816 + EX31_RS11160 0.12 +0.1 163,835 - EX31_RS11160 0.14 -0.1 163,852 - EX31_RS11160 0.16 +0.6 163,866 + EX31_RS11160 0.17 -0.2 163,867 - EX31_RS11160 0.17 -1.9 163,867 - EX31_RS11160 0.17 -0.4 163,867 - EX31_RS11160 0.17 +0.9 163,882 + EX31_RS11160 0.19 +0.3 163,883 - EX31_RS11160 0.19 -0.1 164,019 - EX31_RS11160 0.33 -0.2 164,138 - EX31_RS11160 0.45 +0.3 164,139 + EX31_RS11160 0.45 +0.9 164,139 + EX31_RS11160 0.45 +1.0 164,139 + EX31_RS11160 0.45 -1.9 164,139 + EX31_RS11160 0.45 +0.8 164,140 - EX31_RS11160 0.45 -0.5 164,272 + EX31_RS11160 0.59 +1.4 164,273 - EX31_RS11160 0.59 +0.4 164,496 + EX31_RS11160 0.81 +0.5 164,496 + EX31_RS11160 0.81 +0.6 164,557 + EX31_RS11160 0.88 +0.1 164,658 - -0.7 164,662 + -0.3 164,662 + +0.3 164,662 + -0.3 164,663 - +1.9 164,663 - +0.5 164,663 - +1.9 164,663 - +0.8 164,663 - +1.3
Or see this region's nucleotide sequence