Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06815 and EX31_RS06820 are separated by 18 nucleotides EX31_RS06820 and EX31_RS06825 are separated by 13 nucleotides EX31_RS06825 and EX31_RS06830 are separated by 38 nucleotides EX31_RS06830 and EX31_RS06835 are separated by 21 nucleotides
EX31_RS06815: EX31_RS06815 - argininosuccinate lyase, at 150,616 to 152,043
_RS06815
EX31_RS06820: EX31_RS06820 - amino acid ABC transporter permease, at 152,062 to 152,934
_RS06820
EX31_RS06825: EX31_RS06825 - amino acid ABC transporter ATP-binding protein, at 152,948 to 153,712
_RS06825
EX31_RS06830: EX31_RS06830 - ABC transporter substrate-binding protein, at 153,751 to 154,572
_RS06830
EX31_RS06835: EX31_RS06835 - polysaccharide deacetylase, at 154,594 to 155,448
_RS06835
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2 at 152.017 kb on - strand at 152.018 kb on + strand at 152.018 kb on + strand at 152.018 kb on + strand at 152.019 kb on - strand at 152.024 kb on - strand at 152.039 kb on + strand at 152.128 kb on + strand at 152.128 kb on + strand at 152.143 kb on + strand at 152.147 kb on - strand at 152.233 kb on + strand, within EX31_RS06820 at 152.392 kb on + strand, within EX31_RS06820 at 152.394 kb on + strand, within EX31_RS06820 at 152.394 kb on + strand, within EX31_RS06820 at 152.394 kb on + strand, within EX31_RS06820 at 152.394 kb on + strand, within EX31_RS06820 at 152.563 kb on + strand, within EX31_RS06820 at 152.563 kb on + strand, within EX31_RS06820 at 152.776 kb on + strand, within EX31_RS06820 at 152.776 kb on + strand, within EX31_RS06820 at 152.777 kb on - strand, within EX31_RS06820 at 152.794 kb on + strand, within EX31_RS06820 at 152.794 kb on + strand, within EX31_RS06820 at 152.795 kb on - strand, within EX31_RS06820 at 152.896 kb on + strand at 152.896 kb on + strand at 152.959 kb on + strand at 152.980 kb on + strand at 152.984 kb on + strand at 152.984 kb on + strand at 152.985 kb on - strand at 152.985 kb on - strand at 153.231 kb on + strand, within EX31_RS06825 at 153.231 kb on + strand, within EX31_RS06825 at 153.232 kb on - strand, within EX31_RS06825 at 153.370 kb on + strand, within EX31_RS06825 at 153.573 kb on + strand, within EX31_RS06825 at 153.574 kb on - strand, within EX31_RS06825 at 153.630 kb on + strand, within EX31_RS06825 at 153.631 kb on - strand, within EX31_RS06825 at 153.652 kb on + strand at 153.849 kb on + strand, within EX31_RS06830 at 154.091 kb on - strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.114 kb on + strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.117 kb on - strand, within EX31_RS06830 at 154.227 kb on + strand, within EX31_RS06830 at 154.227 kb on + strand, within EX31_RS06830 at 154.228 kb on - strand, within EX31_RS06830 at 154.351 kb on + strand, within EX31_RS06830 at 154.378 kb on + strand, within EX31_RS06830 at 154.379 kb on - strand, within EX31_RS06830 at 154.622 kb on + strand at 154.622 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 152,017 - -0.7 152,018 + +0.4 152,018 + +1.0 152,018 + -0.4 152,019 - +0.5 152,024 - -0.2 152,039 + +0.9 152,128 + +1.9 152,128 + +0.2 152,143 + +1.3 152,147 - +0.1 152,233 + EX31_RS06820 0.20 +0.3 152,392 + EX31_RS06820 0.38 +0.1 152,394 + EX31_RS06820 0.38 +1.9 152,394 + EX31_RS06820 0.38 +0.3 152,394 + EX31_RS06820 0.38 +0.1 152,394 + EX31_RS06820 0.38 -0.5 152,563 + EX31_RS06820 0.57 +1.1 152,563 + EX31_RS06820 0.57 +1.5 152,776 + EX31_RS06820 0.82 +1.5 152,776 + EX31_RS06820 0.82 +0.8 152,777 - EX31_RS06820 0.82 +0.6 152,794 + EX31_RS06820 0.84 -0.2 152,794 + EX31_RS06820 0.84 +0.1 152,795 - EX31_RS06820 0.84 +1.5 152,896 + -0.9 152,896 + +1.1 152,959 + +1.5 152,980 + -0.5 152,984 + +0.2 152,984 + +0.7 152,985 - -1.8 152,985 - -1.4 153,231 + EX31_RS06825 0.37 -0.7 153,231 + EX31_RS06825 0.37 -2.5 153,232 - EX31_RS06825 0.37 -0.2 153,370 + EX31_RS06825 0.55 +0.1 153,573 + EX31_RS06825 0.82 -2.1 153,574 - EX31_RS06825 0.82 -0.5 153,630 + EX31_RS06825 0.89 -0.5 153,631 - EX31_RS06825 0.89 -0.1 153,652 + -0.6 153,849 + EX31_RS06830 0.12 +0.5 154,091 - EX31_RS06830 0.41 +1.0 154,093 + EX31_RS06830 0.42 -0.2 154,093 + EX31_RS06830 0.42 -0.5 154,093 + EX31_RS06830 0.42 -0.2 154,114 + EX31_RS06830 0.44 -0.8 154,115 - EX31_RS06830 0.44 +2.3 154,115 - EX31_RS06830 0.44 +0.8 154,115 - EX31_RS06830 0.44 -2.5 154,117 - EX31_RS06830 0.45 +1.3 154,227 + EX31_RS06830 0.58 -0.0 154,227 + EX31_RS06830 0.58 -0.5 154,228 - EX31_RS06830 0.58 -0.9 154,351 + EX31_RS06830 0.73 -0.4 154,378 + EX31_RS06830 0.76 -0.9 154,379 - EX31_RS06830 0.76 +0.7 154,622 + -0.3 154,622 + -0.7
Or see this region's nucleotide sequence