Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03680 and EX31_RS03685 are separated by 2 nucleotides EX31_RS03685 and EX31_RS03690 are separated by 1 nucleotides
EX31_RS03680: EX31_RS03680 - 2-isopropylmalate synthase, at 235,390 to 236,979
_RS03680
EX31_RS03685: EX31_RS03685 - 3-isopropylmalate dehydrogenase, at 236,982 to 238,073
_RS03685
EX31_RS03690: EX31_RS03690 - 3-isopropylmalate dehydratase large subunit, at 238,075 to 239,475
_RS03690
Position (kb) 236
237
238
239 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 235.983 kb on + strand, within EX31_RS03680 at 236.025 kb on + strand, within EX31_RS03680 at 236.043 kb on - strand, within EX31_RS03680 at 236.149 kb on + strand, within EX31_RS03680 at 236.150 kb on - strand, within EX31_RS03680 at 236.150 kb on - strand, within EX31_RS03680 at 236.173 kb on - strand, within EX31_RS03680 at 236.173 kb on - strand, within EX31_RS03680 at 236.173 kb on - strand, within EX31_RS03680 at 236.189 kb on - strand, within EX31_RS03680 at 236.440 kb on + strand, within EX31_RS03680 at 236.440 kb on + strand, within EX31_RS03680 at 236.440 kb on + strand, within EX31_RS03680 at 236.440 kb on + strand, within EX31_RS03680 at 236.441 kb on - strand, within EX31_RS03680 at 236.441 kb on - strand, within EX31_RS03680 at 236.441 kb on - strand, within EX31_RS03680 at 236.458 kb on + strand, within EX31_RS03680 at 236.458 kb on + strand, within EX31_RS03680 at 236.458 kb on + strand, within EX31_RS03680 at 236.458 kb on + strand, within EX31_RS03680 at 236.459 kb on - strand, within EX31_RS03680 at 236.459 kb on - strand, within EX31_RS03680 at 236.507 kb on - strand, within EX31_RS03680 at 236.507 kb on - strand, within EX31_RS03680 at 236.560 kb on + strand, within EX31_RS03680 at 236.695 kb on - strand, within EX31_RS03680 at 236.734 kb on + strand, within EX31_RS03680 at 236.735 kb on - strand, within EX31_RS03680 at 236.735 kb on - strand, within EX31_RS03680 at 236.735 kb on - strand, within EX31_RS03680 at 236.867 kb on + strand at 236.867 kb on + strand at 236.867 kb on + strand at 236.867 kb on + strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.873 kb on + strand at 236.873 kb on + strand at 236.873 kb on + strand at 236.874 kb on - strand at 236.880 kb on + strand at 236.880 kb on + strand at 236.880 kb on + strand at 236.881 kb on - strand at 236.961 kb on + strand at 236.999 kb on + strand at 236.999 kb on + strand at 237.000 kb on - strand at 237.051 kb on + strand at 237.051 kb on + strand at 237.052 kb on - strand at 237.052 kb on - strand at 237.052 kb on - strand at 237.052 kb on - strand at 237.070 kb on + strand at 237.070 kb on + strand at 237.071 kb on - strand at 237.266 kb on - strand, within EX31_RS03685 at 237.296 kb on + strand, within EX31_RS03685 at 237.296 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.299 kb on + strand, within EX31_RS03685 at 237.322 kb on - strand, within EX31_RS03685 at 237.410 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.414 kb on + strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.415 kb on - strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.480 kb on + strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.481 kb on - strand, within EX31_RS03685 at 237.484 kb on - strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.590 kb on + strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.591 kb on - strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.654 kb on + strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.655 kb on - strand, within EX31_RS03685 at 237.683 kb on + strand, within EX31_RS03685 at 237.684 kb on - strand, within EX31_RS03685 at 237.858 kb on - strand, within EX31_RS03685 at 237.905 kb on - strand, within EX31_RS03685 at 237.913 kb on - strand, within EX31_RS03685 at 237.962 kb on + strand, within EX31_RS03685 at 237.963 kb on - strand, within EX31_RS03685 at 237.963 kb on - strand, within EX31_RS03685 at 237.997 kb on + strand at 237.997 kb on + strand at 237.997 kb on + strand at 237.998 kb on - strand at 237.998 kb on - strand at 237.998 kb on - strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.071 kb on + strand at 238.072 kb on - strand at 238.072 kb on - strand at 238.072 kb on - strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.115 kb on + strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.116 kb on - strand at 238.148 kb on + strand at 238.149 kb on - strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.150 kb on + strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.151 kb on - strand at 238.239 kb on + strand, within EX31_RS03690 at 238.281 kb on + strand, within EX31_RS03690 at 238.282 kb on - strand, within EX31_RS03690 at 238.282 kb on - strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.366 kb on + strand, within EX31_RS03690 at 238.367 kb on - strand, within EX31_RS03690 at 238.367 kb on - strand, within EX31_RS03690 at 238.385 kb on - strand, within EX31_RS03690 at 238.563 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.620 kb on + strand, within EX31_RS03690 at 238.621 kb on - strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.722 kb on + strand, within EX31_RS03690 at 238.723 kb on - strand, within EX31_RS03690 at 238.723 kb on - strand, within EX31_RS03690 at 238.789 kb on - strand, within EX31_RS03690 at 238.789 kb on - strand, within EX31_RS03690 at 238.906 kb on - strand, within EX31_RS03690 at 238.906 kb on - strand, within EX31_RS03690 at 238.947 kb on + strand, within EX31_RS03690 at 238.948 kb on - strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.047 kb on + strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.048 kb on - strand, within EX31_RS03690 at 239.070 kb on + strand, within EX31_RS03690
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 235,983 + EX31_RS03680 0.37 -2.7 236,025 + EX31_RS03680 0.40 -1.9 236,043 - EX31_RS03680 0.41 -1.2 236,149 + EX31_RS03680 0.48 -1.2 236,150 - EX31_RS03680 0.48 -4.0 236,150 - EX31_RS03680 0.48 -2.5 236,173 - EX31_RS03680 0.49 -2.5 236,173 - EX31_RS03680 0.49 -3.5 236,173 - EX31_RS03680 0.49 -2.9 236,189 - EX31_RS03680 0.50 -1.8 236,440 + EX31_RS03680 0.66 -3.9 236,440 + EX31_RS03680 0.66 -1.4 236,440 + EX31_RS03680 0.66 -3.1 236,440 + EX31_RS03680 0.66 -3.0 236,441 - EX31_RS03680 0.66 -3.6 236,441 - EX31_RS03680 0.66 -3.6 236,441 - EX31_RS03680 0.66 -2.6 236,458 + EX31_RS03680 0.67 -1.8 236,458 + EX31_RS03680 0.67 -2.4 236,458 + EX31_RS03680 0.67 -1.1 236,458 + EX31_RS03680 0.67 -3.0 236,459 - EX31_RS03680 0.67 -1.2 236,459 - EX31_RS03680 0.67 -1.5 236,507 - EX31_RS03680 0.70 -2.6 236,507 - EX31_RS03680 0.70 -4.1 236,560 + EX31_RS03680 0.74 -0.3 236,695 - EX31_RS03680 0.82 -2.3 236,734 + EX31_RS03680 0.85 -1.2 236,735 - EX31_RS03680 0.85 -1.2 236,735 - EX31_RS03680 0.85 -2.8 236,735 - EX31_RS03680 0.85 -2.5 236,867 + -0.4 236,867 + -0.9 236,867 + -0.6 236,867 + -0.2 236,868 - -3.1 236,868 - -3.6 236,868 - -1.4 236,868 - -3.0 236,873 + -1.5 236,873 + +0.4 236,873 + -0.6 236,874 - -3.5 236,880 + -0.0 236,880 + -0.5 236,880 + -1.1 236,881 - -4.9 236,961 + -1.5 236,999 + -2.3 236,999 + -1.4 237,000 - +0.2 237,051 + -2.7 237,051 + -3.1 237,052 - -3.4 237,052 - -2.7 237,052 - -2.2 237,052 - -1.9 237,070 + -4.0 237,070 + -4.1 237,071 - -1.8 237,266 - EX31_RS03685 0.26 -3.4 237,296 + EX31_RS03685 0.29 -3.7 237,296 + EX31_RS03685 0.29 -1.2 237,299 + EX31_RS03685 0.29 -2.5 237,299 + EX31_RS03685 0.29 -2.9 237,299 + EX31_RS03685 0.29 -2.9 237,322 - EX31_RS03685 0.31 -2.7 237,410 + EX31_RS03685 0.39 -3.4 237,414 + EX31_RS03685 0.40 -2.4 237,414 + EX31_RS03685 0.40 -4.5 237,414 + EX31_RS03685 0.40 -1.9 237,414 + EX31_RS03685 0.40 -1.9 237,414 + EX31_RS03685 0.40 -4.3 237,415 - EX31_RS03685 0.40 -2.8 237,415 - EX31_RS03685 0.40 -1.6 237,415 - EX31_RS03685 0.40 -2.6 237,415 - EX31_RS03685 0.40 -2.9 237,415 - EX31_RS03685 0.40 +0.2 237,415 - EX31_RS03685 0.40 -1.2 237,480 + EX31_RS03685 0.46 -3.9 237,480 + EX31_RS03685 0.46 -2.9 237,480 + EX31_RS03685 0.46 -2.4 237,480 + EX31_RS03685 0.46 -2.5 237,480 + EX31_RS03685 0.46 -1.8 237,481 - EX31_RS03685 0.46 +0.2 237,481 - EX31_RS03685 0.46 -3.2 237,481 - EX31_RS03685 0.46 -4.1 237,484 - EX31_RS03685 0.46 -1.4 237,590 + EX31_RS03685 0.56 -3.0 237,590 + EX31_RS03685 0.56 -1.6 237,590 + EX31_RS03685 0.56 -3.9 237,590 + EX31_RS03685 0.56 -2.5 237,591 - EX31_RS03685 0.56 -3.6 237,591 - EX31_RS03685 0.56 -2.3 237,591 - EX31_RS03685 0.56 -1.7 237,591 - EX31_RS03685 0.56 -2.9 237,591 - EX31_RS03685 0.56 -1.9 237,654 + EX31_RS03685 0.62 -4.0 237,654 + EX31_RS03685 0.62 -3.0 237,654 + EX31_RS03685 0.62 -2.6 237,654 + EX31_RS03685 0.62 -3.6 237,654 + EX31_RS03685 0.62 -2.5 237,654 + EX31_RS03685 0.62 -1.9 237,654 + EX31_RS03685 0.62 -4.4 237,654 + EX31_RS03685 0.62 -3.8 237,654 + EX31_RS03685 0.62 -2.6 237,654 + EX31_RS03685 0.62 -3.0 237,654 + EX31_RS03685 0.62 -3.1 237,654 + EX31_RS03685 0.62 -3.1 237,654 + EX31_RS03685 0.62 -2.9 237,654 + EX31_RS03685 0.62 -2.0 237,654 + EX31_RS03685 0.62 -1.2 237,654 + EX31_RS03685 0.62 -3.5 237,654 + EX31_RS03685 0.62 -1.3 237,654 + EX31_RS03685 0.62 -2.3 237,655 - EX31_RS03685 0.62 -1.9 237,655 - EX31_RS03685 0.62 -4.2 237,655 - EX31_RS03685 0.62 -1.6 237,655 - EX31_RS03685 0.62 +2.2 237,655 - EX31_RS03685 0.62 -1.4 237,655 - EX31_RS03685 0.62 -2.0 237,655 - EX31_RS03685 0.62 -3.6 237,655 - EX31_RS03685 0.62 -0.6 237,655 - EX31_RS03685 0.62 -0.6 237,655 - EX31_RS03685 0.62 -2.9 237,655 - EX31_RS03685 0.62 -1.6 237,655 - EX31_RS03685 0.62 -3.9 237,655 - EX31_RS03685 0.62 -1.2 237,655 - EX31_RS03685 0.62 -1.9 237,655 - EX31_RS03685 0.62 +0.2 237,683 + EX31_RS03685 0.64 -2.6 237,684 - EX31_RS03685 0.64 -3.4 237,858 - EX31_RS03685 0.80 -2.5 237,905 - EX31_RS03685 0.85 -3.4 237,913 - EX31_RS03685 0.85 -1.8 237,962 + EX31_RS03685 0.90 -2.2 237,963 - EX31_RS03685 0.90 -2.3 237,963 - EX31_RS03685 0.90 -3.4 237,997 + -1.6 237,997 + -3.2 237,997 + -3.9 237,998 - -2.8 237,998 - -2.4 237,998 - -2.8 238,071 + -3.4 238,071 + -2.0 238,071 + -2.8 238,071 + -3.7 238,072 - -2.9 238,072 - -3.7 238,072 - -3.1 238,115 + -2.9 238,115 + -2.3 238,115 + -2.4 238,115 + -3.0 238,115 + -4.2 238,115 + -2.0 238,115 + -2.2 238,115 + -0.8 238,115 + -2.0 238,116 - -2.4 238,116 - -2.7 238,116 - -3.3 238,116 - -3.4 238,116 - -0.4 238,116 - -1.9 238,148 + -2.5 238,149 - -0.6 238,150 + -4.3 238,150 + -2.6 238,150 + -2.9 238,150 + -3.5 238,150 + -1.9 238,150 + -3.0 238,150 + -2.8 238,150 + -3.7 238,150 + -2.4 238,150 + -1.5 238,150 + -2.1 238,150 + -1.5 238,151 - -2.3 238,151 - -2.7 238,151 - -3.1 238,151 - -1.4 238,151 - -3.5 238,151 - -3.0 238,151 - -1.2 238,151 - -3.2 238,151 - -1.6 238,239 + EX31_RS03690 0.12 -2.1 238,281 + EX31_RS03690 0.15 -4.2 238,282 - EX31_RS03690 0.15 -3.6 238,282 - EX31_RS03690 0.15 -1.7 238,366 + EX31_RS03690 0.21 -3.0 238,366 + EX31_RS03690 0.21 -3.1 238,366 + EX31_RS03690 0.21 -2.8 238,367 - EX31_RS03690 0.21 -2.0 238,367 - EX31_RS03690 0.21 -3.6 238,385 - EX31_RS03690 0.22 -1.9 238,563 + EX31_RS03690 0.35 -3.1 238,620 + EX31_RS03690 0.39 -2.5 238,620 + EX31_RS03690 0.39 -2.5 238,620 + EX31_RS03690 0.39 -0.6 238,620 + EX31_RS03690 0.39 -2.4 238,621 - EX31_RS03690 0.39 -2.7 238,722 + EX31_RS03690 0.46 -0.9 238,722 + EX31_RS03690 0.46 -2.4 238,722 + EX31_RS03690 0.46 -0.7 238,723 - EX31_RS03690 0.46 -2.2 238,723 - EX31_RS03690 0.46 -0.9 238,789 - EX31_RS03690 0.51 -2.2 238,789 - EX31_RS03690 0.51 -0.4 238,906 - EX31_RS03690 0.59 -3.7 238,906 - EX31_RS03690 0.59 -2.3 238,947 + EX31_RS03690 0.62 -3.1 238,948 - EX31_RS03690 0.62 -2.2 239,047 + EX31_RS03690 0.69 -2.9 239,047 + EX31_RS03690 0.69 -3.4 239,047 + EX31_RS03690 0.69 -4.0 239,047 + EX31_RS03690 0.69 -1.8 239,047 + EX31_RS03690 0.69 -1.1 239,047 + EX31_RS03690 0.69 -3.3 239,047 + EX31_RS03690 0.69 -2.2 239,048 - EX31_RS03690 0.69 -1.6 239,048 - EX31_RS03690 0.69 -3.9 239,048 - EX31_RS03690 0.69 -3.3 239,048 - EX31_RS03690 0.69 -1.9 239,048 - EX31_RS03690 0.69 -2.2 239,048 - EX31_RS03690 0.69 -2.8 239,048 - EX31_RS03690 0.69 -2.1 239,048 - EX31_RS03690 0.69 -1.2 239,048 - EX31_RS03690 0.69 -3.1 239,048 - EX31_RS03690 0.69 -2.3 239,070 + EX31_RS03690 0.71 -0.9
Or see this region's nucleotide sequence