Experiment: Ammonium chloride (N); ShewMM media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01495 and EX31_RS01500 are separated by 103 nucleotides EX31_RS01500 and EX31_RS01505 are separated by 211 nucleotides EX31_RS01505 and EX31_RS01510 are separated by 108 nucleotides EX31_RS01510 and EX31_RS01515 are separated by 49 nucleotides
EX31_RS01495: EX31_RS01495 - multidrug efflux RND transporter permease subunit, at 335,883 to 339,035
_RS01495
EX31_RS01500: EX31_RS01500 - transcriptional regulator, at 339,139 to 339,624
_RS01500
EX31_RS01505: EX31_RS01505 - porin, at 339,836 to 340,438
_RS01505
EX31_RS01510: EX31_RS01510 - DinI-like family protein, at 340,547 to 340,750
_RS01510
EX31_RS01515: EX31_RS01515 - DinI-like family protein, at 340,800 to 341,039
_RS01515
Position (kb) 339
340
341 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 339.002 kb on + strand at 339.033 kb on + strand at 339.092 kb on + strand at 339.092 kb on + strand at 339.094 kb on + strand at 339.095 kb on - strand at 339.095 kb on - strand at 339.130 kb on - strand at 339.198 kb on - strand, within EX31_RS01500 at 339.198 kb on - strand, within EX31_RS01500 at 339.198 kb on - strand, within EX31_RS01500 at 339.300 kb on - strand, within EX31_RS01500 at 339.367 kb on - strand, within EX31_RS01500 at 339.367 kb on - strand, within EX31_RS01500 at 339.387 kb on - strand, within EX31_RS01500 at 339.387 kb on - strand, within EX31_RS01500 at 339.533 kb on - strand, within EX31_RS01500 at 339.598 kb on - strand at 339.598 kb on - strand at 339.598 kb on - strand at 339.598 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.651 kb on - strand at 339.651 kb on - strand at 339.684 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.702 kb on - strand at 339.705 kb on - strand at 339.705 kb on - strand at 339.707 kb on - strand at 339.745 kb on - strand at 339.767 kb on + strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.838 kb on - strand at 339.846 kb on + strand at 339.846 kb on + strand at 339.847 kb on - strand at 339.851 kb on + strand at 339.852 kb on - strand at 339.852 kb on - strand at 339.852 kb on - strand at 339.925 kb on + strand, within EX31_RS01505 at 339.934 kb on - strand, within EX31_RS01505 at 339.942 kb on + strand, within EX31_RS01505 at 339.943 kb on - strand, within EX31_RS01505 at 339.952 kb on + strand, within EX31_RS01505 at 339.967 kb on - strand, within EX31_RS01505 at 339.967 kb on - strand, within EX31_RS01505 at 339.970 kb on - strand, within EX31_RS01505 at 339.990 kb on + strand, within EX31_RS01505 at 339.990 kb on + strand, within EX31_RS01505 at 339.991 kb on - strand, within EX31_RS01505 at 339.991 kb on - strand, within EX31_RS01505 at 339.994 kb on + strand, within EX31_RS01505 at 340.024 kb on + strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.057 kb on + strand, within EX31_RS01505 at 340.058 kb on - strand, within EX31_RS01505 at 340.058 kb on - strand, within EX31_RS01505 at 340.069 kb on - strand, within EX31_RS01505 at 340.069 kb on - strand, within EX31_RS01505 at 340.115 kb on + strand, within EX31_RS01505 at 340.185 kb on + strand, within EX31_RS01505 at 340.186 kb on - strand, within EX31_RS01505 at 340.186 kb on - strand, within EX31_RS01505 at 340.200 kb on + strand, within EX31_RS01505 at 340.201 kb on - strand, within EX31_RS01505 at 340.218 kb on + strand, within EX31_RS01505 at 340.219 kb on - strand, within EX31_RS01505 at 340.297 kb on + strand, within EX31_RS01505 at 340.297 kb on + strand, within EX31_RS01505 at 340.298 kb on - strand, within EX31_RS01505 at 340.314 kb on + strand, within EX31_RS01505 at 340.315 kb on - strand, within EX31_RS01505 at 340.373 kb on + strand, within EX31_RS01505 at 340.393 kb on + strand at 340.423 kb on - strand at 340.460 kb on - strand at 340.460 kb on - strand at 340.510 kb on + strand at 340.546 kb on + strand at 340.546 kb on + strand at 340.556 kb on - strand at 340.635 kb on + strand, within EX31_RS01510 at 340.636 kb on - strand, within EX31_RS01510 at 340.639 kb on + strand, within EX31_RS01510 at 340.639 kb on + strand, within EX31_RS01510 at 340.640 kb on - strand, within EX31_RS01510 at 340.668 kb on + strand, within EX31_RS01510 at 340.669 kb on - strand, within EX31_RS01510 at 340.727 kb on - strand, within EX31_RS01510 at 340.749 kb on - strand at 340.749 kb on - strand at 340.749 kb on - strand at 340.768 kb on + strand at 340.769 kb on - strand at 340.814 kb on + strand at 340.820 kb on - strand at 340.823 kb on + strand at 340.823 kb on + strand at 340.853 kb on - strand, within EX31_RS01515 at 340.988 kb on - strand, within EX31_RS01515 at 340.988 kb on - strand, within EX31_RS01515 at 340.988 kb on - strand, within EX31_RS01515 at 340.988 kb on - strand, within EX31_RS01515 at 340.990 kb on + strand, within EX31_RS01515 at 340.990 kb on + strand, within EX31_RS01515 at 340.990 kb on + strand, within EX31_RS01515 at 340.991 kb on - strand, within EX31_RS01515 at 341.012 kb on + strand, within EX31_RS01515 at 341.012 kb on + strand, within EX31_RS01515 at 341.013 kb on - strand, within EX31_RS01515 at 341.082 kb on - strand at 341.147 kb on + strand at 341.147 kb on + strand at 341.164 kb on + strand at 341.179 kb on + strand at 341.179 kb on + strand at 341.184 kb on + strand at 341.200 kb on + strand at 341.200 kb on + strand at 341.200 kb on + strand at 341.200 kb on + strand at 341.201 kb on - strand at 341.209 kb on - strand at 341.211 kb on + strand at 341.222 kb on + strand at 341.225 kb on + strand at 341.257 kb on + strand at 341.257 kb on + strand at 341.257 kb on + strand at 341.257 kb on + strand at 341.257 kb on + strand at 341.258 kb on - strand at 341.258 kb on - strand at 341.277 kb on + strand at 341.277 kb on + strand at 341.277 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.302 kb on + strand at 341.311 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.314 kb on + strand at 341.320 kb on + strand at 341.320 kb on + strand at 341.320 kb on + strand at 341.320 kb on + strand at 341.320 kb on + strand at 341.338 kb on + strand at 341.338 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.359 kb on - strand at 341.359 kb on - strand at 341.367 kb on - strand at 341.416 kb on + strand at 341.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; shaking remove 339,002 + -0.4 339,033 + -0.2 339,092 + +1.6 339,092 + -0.2 339,094 + -0.4 339,095 - +0.8 339,095 - -0.1 339,130 - +0.4 339,198 - EX31_RS01500 0.12 -0.1 339,198 - EX31_RS01500 0.12 -0.8 339,198 - EX31_RS01500 0.12 +1.5 339,300 - EX31_RS01500 0.33 +0.6 339,367 - EX31_RS01500 0.47 +0.1 339,367 - EX31_RS01500 0.47 +0.8 339,387 - EX31_RS01500 0.51 -0.5 339,387 - EX31_RS01500 0.51 -1.8 339,533 - EX31_RS01500 0.81 -2.9 339,598 - -2.9 339,598 - -2.9 339,598 - -1.7 339,598 - -0.4 339,620 - -0.6 339,620 - -0.8 339,620 - +0.3 339,620 - +0.5 339,651 - +1.6 339,651 - -1.8 339,684 - +1.1 339,686 - -0.8 339,686 - -1.2 339,686 - +1.0 339,686 - +0.1 339,686 - -0.2 339,686 - -0.1 339,686 - -0.8 339,686 - +1.2 339,686 - -0.6 339,686 - +0.8 339,686 - +1.0 339,686 - -0.2 339,686 - +1.1 339,702 - +0.1 339,705 - +0.1 339,705 - -0.9 339,707 - +0.4 339,745 - -0.3 339,767 + -0.0 339,768 - +0.6 339,768 - -0.2 339,768 - -0.4 339,768 - +0.5 339,838 - +0.3 339,846 + -0.6 339,846 + +1.7 339,847 - -0.3 339,851 + +1.1 339,852 - -0.7 339,852 - -0.5 339,852 - +1.0 339,925 + EX31_RS01505 0.15 +0.1 339,934 - EX31_RS01505 0.16 -3.2 339,942 + EX31_RS01505 0.18 -1.0 339,943 - EX31_RS01505 0.18 +0.5 339,952 + EX31_RS01505 0.19 -0.7 339,967 - EX31_RS01505 0.22 -0.5 339,967 - EX31_RS01505 0.22 -0.8 339,970 - EX31_RS01505 0.22 -0.3 339,990 + EX31_RS01505 0.26 +0.3 339,990 + EX31_RS01505 0.26 +0.6 339,991 - EX31_RS01505 0.26 -0.9 339,991 - EX31_RS01505 0.26 +0.7 339,994 + EX31_RS01505 0.26 -0.5 340,024 + EX31_RS01505 0.31 -0.7 340,025 - EX31_RS01505 0.31 +0.9 340,025 - EX31_RS01505 0.31 -1.0 340,025 - EX31_RS01505 0.31 -0.4 340,025 - EX31_RS01505 0.31 -0.5 340,057 + EX31_RS01505 0.37 +3.1 340,058 - EX31_RS01505 0.37 +1.1 340,058 - EX31_RS01505 0.37 +0.5 340,069 - EX31_RS01505 0.39 -1.1 340,069 - EX31_RS01505 0.39 +0.2 340,115 + EX31_RS01505 0.46 -0.9 340,185 + EX31_RS01505 0.58 +0.7 340,186 - EX31_RS01505 0.58 +1.6 340,186 - EX31_RS01505 0.58 -0.7 340,200 + EX31_RS01505 0.60 -1.0 340,201 - EX31_RS01505 0.61 -0.9 340,218 + EX31_RS01505 0.63 -0.8 340,219 - EX31_RS01505 0.64 -1.4 340,297 + EX31_RS01505 0.76 +1.3 340,297 + EX31_RS01505 0.76 +1.2 340,298 - EX31_RS01505 0.77 +1.6 340,314 + EX31_RS01505 0.79 +0.4 340,315 - EX31_RS01505 0.79 -0.2 340,373 + EX31_RS01505 0.89 +0.7 340,393 + +0.7 340,423 - +2.7 340,460 - +1.1 340,460 - -0.7 340,510 + -0.7 340,546 + -1.8 340,546 + -0.6 340,556 - +0.8 340,635 + EX31_RS01510 0.43 -1.2 340,636 - EX31_RS01510 0.44 +2.1 340,639 + EX31_RS01510 0.45 -0.4 340,639 + EX31_RS01510 0.45 +0.5 340,640 - EX31_RS01510 0.46 -0.3 340,668 + EX31_RS01510 0.59 -0.2 340,669 - EX31_RS01510 0.60 -0.5 340,727 - EX31_RS01510 0.88 -0.2 340,749 - +1.7 340,749 - +0.3 340,749 - +1.1 340,768 + +0.8 340,769 - +0.2 340,814 + +1.5 340,820 - -2.3 340,823 + +0.7 340,823 + -0.7 340,853 - EX31_RS01515 0.22 +1.1 340,988 - EX31_RS01515 0.78 +0.2 340,988 - EX31_RS01515 0.78 +0.6 340,988 - EX31_RS01515 0.78 +0.1 340,988 - EX31_RS01515 0.78 -1.2 340,990 + EX31_RS01515 0.79 +0.7 340,990 + EX31_RS01515 0.79 -0.4 340,990 + EX31_RS01515 0.79 +1.1 340,991 - EX31_RS01515 0.80 -0.7 341,012 + EX31_RS01515 0.88 +1.1 341,012 + EX31_RS01515 0.88 +0.7 341,013 - EX31_RS01515 0.89 -0.2 341,082 - +0.1 341,147 + -0.5 341,147 + -0.7 341,164 + -0.7 341,179 + -0.7 341,179 + +0.4 341,184 + -1.5 341,200 + +0.5 341,200 + +1.3 341,200 + +0.2 341,200 + -1.1 341,201 - -0.2 341,209 - +1.1 341,211 + +0.7 341,222 + +0.8 341,225 + +0.1 341,257 + +0.5 341,257 + +0.9 341,257 + +0.2 341,257 + +0.5 341,257 + -1.2 341,258 - +0.5 341,258 - +1.0 341,277 + -0.6 341,277 + +0.5 341,277 + +0.8 341,302 + +0.9 341,302 + +1.2 341,302 + +1.1 341,302 + -0.6 341,302 + +0.2 341,302 + +0.3 341,302 + -0.0 341,311 + +0.7 341,314 + +0.3 341,314 + -0.1 341,314 + +0.3 341,314 + +2.7 341,314 + +1.8 341,314 + +1.0 341,314 + +0.9 341,314 + +0.7 341,314 + -0.2 341,314 + -0.2 341,320 + -0.6 341,320 + -0.3 341,320 + -0.1 341,320 + +0.2 341,320 + -0.2 341,338 + +0.5 341,338 + -1.7 341,358 + +0.9 341,358 + +1.0 341,358 + +0.1 341,358 + -0.7 341,358 + -0.2 341,358 + +1.0 341,358 + +0.7 341,358 + +0.4 341,359 - +0.1 341,359 - +1.6 341,367 - -1.0 341,416 + -0.2 341,417 - +0.8
Or see this region's nucleotide sequence