Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22715 and CA265_RS22720 are separated by 75 nucleotides CA265_RS22720 and CA265_RS22725 are separated by 18 nucleotides CA265_RS22725 and CA265_RS22730 are separated by 121 nucleotides
CA265_RS22715: CA265_RS22715 - acyl-CoA dehydrogenase, at 5,560,184 to 5,561,323
_RS22715
CA265_RS22720: CA265_RS22720 - hypothetical protein, at 5,561,399 to 5,561,743
_RS22720
CA265_RS22725: CA265_RS22725 - hypothetical protein, at 5,561,762 to 5,562,193
_RS22725
CA265_RS22730: CA265_RS22730 - ribosome assembly cofactor RimP, at 5,562,315 to 5,562,779
_RS22730
Position (kb) 5561
5562 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5560.454 kb on - strand, within CA265_RS22715 at 5560.515 kb on + strand, within CA265_RS22715 at 5560.586 kb on - strand, within CA265_RS22715 at 5560.622 kb on + strand, within CA265_RS22715 at 5560.625 kb on + strand, within CA265_RS22715 at 5560.626 kb on - strand, within CA265_RS22715 at 5560.632 kb on + strand, within CA265_RS22715 at 5560.718 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.726 kb on + strand, within CA265_RS22715 at 5560.735 kb on + strand, within CA265_RS22715 at 5560.818 kb on + strand, within CA265_RS22715 at 5560.826 kb on - strand, within CA265_RS22715 at 5560.940 kb on - strand, within CA265_RS22715 at 5560.973 kb on + strand, within CA265_RS22715 at 5560.974 kb on - strand, within CA265_RS22715 at 5560.979 kb on - strand, within CA265_RS22715 at 5561.119 kb on + strand, within CA265_RS22715 at 5561.120 kb on - strand, within CA265_RS22715 at 5561.120 kb on - strand, within CA265_RS22715 at 5561.147 kb on + strand, within CA265_RS22715 at 5561.193 kb on + strand, within CA265_RS22715 at 5561.223 kb on - strand at 5561.238 kb on + strand at 5561.276 kb on - strand at 5561.317 kb on - strand at 5561.322 kb on - strand at 5561.322 kb on - strand at 5561.373 kb on + strand at 5561.400 kb on + strand at 5561.448 kb on - strand, within CA265_RS22720 at 5561.448 kb on - strand, within CA265_RS22720 at 5561.520 kb on - strand, within CA265_RS22720 at 5561.542 kb on + strand, within CA265_RS22720 at 5561.542 kb on + strand, within CA265_RS22720 at 5561.588 kb on + strand, within CA265_RS22720 at 5561.734 kb on + strand at 5561.734 kb on + strand at 5561.760 kb on - strand at 5561.809 kb on - strand, within CA265_RS22725 at 5561.831 kb on - strand, within CA265_RS22725 at 5561.945 kb on - strand, within CA265_RS22725 at 5561.995 kb on - strand, within CA265_RS22725 at 5561.995 kb on - strand, within CA265_RS22725 at 5562.012 kb on + strand, within CA265_RS22725 at 5562.019 kb on + strand, within CA265_RS22725 at 5562.025 kb on + strand, within CA265_RS22725 at 5562.031 kb on - strand, within CA265_RS22725 at 5562.048 kb on + strand, within CA265_RS22725 at 5562.049 kb on - strand, within CA265_RS22725 at 5562.102 kb on - strand, within CA265_RS22725 at 5562.185 kb on - strand at 5562.206 kb on + strand at 5562.207 kb on - strand at 5562.391 kb on + strand, within CA265_RS22730 at 5562.391 kb on + strand, within CA265_RS22730 at 5562.415 kb on + strand, within CA265_RS22730 at 5562.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,560,454 - CA265_RS22715 0.24 +0.4 5,560,515 + CA265_RS22715 0.29 -0.1 5,560,586 - CA265_RS22715 0.35 +1.7 5,560,622 + CA265_RS22715 0.38 -0.7 5,560,625 + CA265_RS22715 0.39 -0.5 5,560,626 - CA265_RS22715 0.39 -0.6 5,560,632 + CA265_RS22715 0.39 +0.4 5,560,718 - CA265_RS22715 0.47 -0.4 5,560,724 - CA265_RS22715 0.47 -0.2 5,560,724 - CA265_RS22715 0.47 -0.5 5,560,724 - CA265_RS22715 0.47 +0.4 5,560,726 + CA265_RS22715 0.48 +1.2 5,560,735 + CA265_RS22715 0.48 -0.3 5,560,818 + CA265_RS22715 0.56 -1.6 5,560,826 - CA265_RS22715 0.56 +0.4 5,560,940 - CA265_RS22715 0.66 +0.6 5,560,973 + CA265_RS22715 0.69 -0.8 5,560,974 - CA265_RS22715 0.69 -0.2 5,560,979 - CA265_RS22715 0.70 +1.3 5,561,119 + CA265_RS22715 0.82 +0.7 5,561,120 - CA265_RS22715 0.82 +0.6 5,561,120 - CA265_RS22715 0.82 -0.1 5,561,147 + CA265_RS22715 0.84 +0.5 5,561,193 + CA265_RS22715 0.89 -0.4 5,561,223 - +0.2 5,561,238 + +0.2 5,561,276 - -0.4 5,561,317 - +2.3 5,561,322 - +0.7 5,561,322 - -0.1 5,561,373 + -0.1 5,561,400 + +1.1 5,561,448 - CA265_RS22720 0.14 -0.4 5,561,448 - CA265_RS22720 0.14 +0.6 5,561,520 - CA265_RS22720 0.35 +0.5 5,561,542 + CA265_RS22720 0.41 +0.8 5,561,542 + CA265_RS22720 0.41 -0.1 5,561,588 + CA265_RS22720 0.55 +0.4 5,561,734 + +0.1 5,561,734 + +0.3 5,561,760 - -1.1 5,561,809 - CA265_RS22725 0.11 +1.2 5,561,831 - CA265_RS22725 0.16 +0.1 5,561,945 - CA265_RS22725 0.42 -0.3 5,561,995 - CA265_RS22725 0.54 -0.4 5,561,995 - CA265_RS22725 0.54 -0.7 5,562,012 + CA265_RS22725 0.58 -2.7 5,562,019 + CA265_RS22725 0.59 +0.8 5,562,025 + CA265_RS22725 0.61 -0.3 5,562,031 - CA265_RS22725 0.62 +0.3 5,562,048 + CA265_RS22725 0.66 +1.3 5,562,049 - CA265_RS22725 0.66 -0.0 5,562,102 - CA265_RS22725 0.79 -1.1 5,562,185 - -0.5 5,562,206 + +0.2 5,562,207 - +0.0 5,562,391 + CA265_RS22730 0.16 -0.0 5,562,391 + CA265_RS22730 0.16 -0.8 5,562,415 + CA265_RS22730 0.22 +1.1 5,562,743 + -1.6
Or see this region's nucleotide sequence