Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12735 and CA265_RS12740 overlap by 11 nucleotides CA265_RS12740 and CA265_RS12745 are separated by 134 nucleotides CA265_RS12745 and CA265_RS12750 are separated by 435 nucleotides
CA265_RS12735: CA265_RS12735 - RNA polymerase subunit sigma-70, at 3,173,940 to 3,174,443
_RS12735
CA265_RS12740: CA265_RS12740 - hypothetical protein, at 3,174,433 to 3,175,038
_RS12740
CA265_RS12745: CA265_RS12745 - NUDIX hydrolase, at 3,175,173 to 3,175,784
_RS12745
CA265_RS12750: CA265_RS12750 - hypothetical protein, at 3,176,220 to 3,177,086
_RS12750
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3174.182 kb on + strand, within CA265_RS12735 at 3174.182 kb on + strand, within CA265_RS12735 at 3174.183 kb on - strand, within CA265_RS12735 at 3174.211 kb on + strand, within CA265_RS12735 at 3174.255 kb on + strand, within CA265_RS12735 at 3174.256 kb on - strand, within CA265_RS12735 at 3174.261 kb on + strand, within CA265_RS12735 at 3174.304 kb on + strand, within CA265_RS12735 at 3174.418 kb on + strand at 3174.634 kb on - strand, within CA265_RS12740 at 3174.811 kb on + strand, within CA265_RS12740 at 3174.813 kb on + strand, within CA265_RS12740 at 3174.911 kb on - strand, within CA265_RS12740 at 3174.918 kb on - strand, within CA265_RS12740 at 3174.954 kb on - strand, within CA265_RS12740 at 3174.978 kb on - strand at 3175.060 kb on - strand at 3175.071 kb on + strand at 3175.072 kb on - strand at 3175.072 kb on - strand at 3175.099 kb on - strand at 3175.119 kb on + strand at 3175.174 kb on + strand at 3175.175 kb on - strand at 3175.175 kb on - strand at 3175.175 kb on - strand at 3175.176 kb on + strand at 3175.177 kb on - strand at 3175.205 kb on + strand at 3175.205 kb on + strand at 3175.206 kb on - strand at 3175.206 kb on - strand at 3175.206 kb on - strand at 3175.226 kb on + strand at 3175.227 kb on - strand at 3175.356 kb on - strand, within CA265_RS12745 at 3175.407 kb on - strand, within CA265_RS12745 at 3175.517 kb on + strand, within CA265_RS12745 at 3175.525 kb on + strand, within CA265_RS12745 at 3175.526 kb on - strand, within CA265_RS12745 at 3175.568 kb on + strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.629 kb on - strand, within CA265_RS12745 at 3175.762 kb on + strand at 3175.802 kb on - strand at 3175.831 kb on + strand at 3175.832 kb on - strand at 3175.875 kb on + strand at 3175.876 kb on - strand at 3175.876 kb on - strand at 3175.877 kb on + strand at 3175.879 kb on + strand at 3175.879 kb on + strand at 3175.901 kb on - strand at 3175.921 kb on + strand at 3175.922 kb on - strand at 3175.927 kb on + strand at 3175.931 kb on - strand at 3175.950 kb on + strand at 3175.958 kb on + strand at 3175.964 kb on + strand at 3175.968 kb on + strand at 3176.035 kb on - strand at 3176.083 kb on + strand at 3176.108 kb on - strand at 3176.131 kb on - strand at 3176.259 kb on + strand at 3176.353 kb on + strand, within CA265_RS12750 at 3176.354 kb on - strand, within CA265_RS12750 at 3176.403 kb on + strand, within CA265_RS12750 at 3176.424 kb on + strand, within CA265_RS12750 at 3176.424 kb on + strand, within CA265_RS12750 at 3176.429 kb on + strand, within CA265_RS12750 at 3176.447 kb on + strand, within CA265_RS12750 at 3176.500 kb on + strand, within CA265_RS12750 at 3176.543 kb on + strand, within CA265_RS12750 at 3176.543 kb on + strand, within CA265_RS12750 at 3176.544 kb on - strand, within CA265_RS12750 at 3176.609 kb on - strand, within CA265_RS12750 at 3176.627 kb on - strand, within CA265_RS12750 at 3176.630 kb on + strand, within CA265_RS12750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 3,174,182 + CA265_RS12735 0.48 -0.6 3,174,182 + CA265_RS12735 0.48 +0.1 3,174,183 - CA265_RS12735 0.48 -0.1 3,174,211 + CA265_RS12735 0.54 +0.6 3,174,255 + CA265_RS12735 0.62 +0.4 3,174,256 - CA265_RS12735 0.63 +0.5 3,174,261 + CA265_RS12735 0.64 -0.4 3,174,304 + CA265_RS12735 0.72 -0.1 3,174,418 + +0.1 3,174,634 - CA265_RS12740 0.33 -1.0 3,174,811 + CA265_RS12740 0.62 -0.6 3,174,813 + CA265_RS12740 0.63 -0.6 3,174,911 - CA265_RS12740 0.79 -1.9 3,174,918 - CA265_RS12740 0.80 -2.3 3,174,954 - CA265_RS12740 0.86 -0.9 3,174,978 - -0.6 3,175,060 - -0.1 3,175,071 + +0.4 3,175,072 - +0.3 3,175,072 - +0.3 3,175,099 - -0.2 3,175,119 + +0.5 3,175,174 + -1.6 3,175,175 - +0.2 3,175,175 - +0.0 3,175,175 - -0.2 3,175,176 + -0.5 3,175,177 - +0.0 3,175,205 + +0.1 3,175,205 + -0.1 3,175,206 - -0.7 3,175,206 - -1.1 3,175,206 - +0.7 3,175,226 + -0.1 3,175,227 - -0.1 3,175,356 - CA265_RS12745 0.30 +0.4 3,175,407 - CA265_RS12745 0.38 -0.0 3,175,517 + CA265_RS12745 0.56 +0.9 3,175,525 + CA265_RS12745 0.58 +0.1 3,175,526 - CA265_RS12745 0.58 +0.4 3,175,568 + CA265_RS12745 0.65 -0.1 3,175,575 - CA265_RS12745 0.66 -2.8 3,175,575 - CA265_RS12745 0.66 +0.8 3,175,575 - CA265_RS12745 0.66 +0.2 3,175,629 - CA265_RS12745 0.75 +0.9 3,175,762 + +0.2 3,175,802 - -0.5 3,175,831 + -1.0 3,175,832 - -0.9 3,175,875 + -0.5 3,175,876 - +0.4 3,175,876 - -0.4 3,175,877 + -0.6 3,175,879 + -1.0 3,175,879 + +0.2 3,175,901 - +0.3 3,175,921 + -0.9 3,175,922 - -0.2 3,175,927 + +0.4 3,175,931 - -0.0 3,175,950 + +0.2 3,175,958 + -0.2 3,175,964 + +0.1 3,175,968 + -0.9 3,176,035 - -0.1 3,176,083 + -0.5 3,176,108 - +0.6 3,176,131 - +0.1 3,176,259 + -0.4 3,176,353 + CA265_RS12750 0.15 +1.4 3,176,354 - CA265_RS12750 0.15 +0.3 3,176,403 + CA265_RS12750 0.21 +0.3 3,176,424 + CA265_RS12750 0.24 +0.5 3,176,424 + CA265_RS12750 0.24 +0.3 3,176,429 + CA265_RS12750 0.24 -0.2 3,176,447 + CA265_RS12750 0.26 -0.1 3,176,500 + CA265_RS12750 0.32 +1.0 3,176,543 + CA265_RS12750 0.37 +0.6 3,176,543 + CA265_RS12750 0.37 -0.1 3,176,544 - CA265_RS12750 0.37 +0.4 3,176,609 - CA265_RS12750 0.45 +0.6 3,176,627 - CA265_RS12750 0.47 -0.1 3,176,630 + CA265_RS12750 0.47 +0.4
Or see this region's nucleotide sequence